Abstract:
:Single-molecule fluorescence methods, particularly single-molecule FRET (fluorescence resonance energy transfer), have provided novel insights into the structure, interactions and dynamics of biological systems. ALEX (alternating-laser excitation) spectroscopy is a new method that extends single-molecule FRET by providing simultaneous information about structure and stoichiometry; this new information allows the detection of interactions in the absence of FRET and extends the dynamic range of distance measurements that are accessible through FRET. In the present article, we discuss combinations of ALEX with confocal microscopy for studying in-solution and in-gel molecules; we also discuss combining ALEX with TIRF (total internal reflection fluorescence) for studying surface-immobilized molecules. We also highlight applications of ALEX to the study of protein-nucleic acid interactions.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Santoso Y,Hwang LC,Le Reste L,Kapanidis ANdoi
10.1042/BST0360738subject
Has Abstractpub_date
2008-08-01 00:00:00pages
738-44issue
Pt 4eissn
0300-5127issn
1470-8752pii
BST0360738journal_volume
36pub_type
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