Abstract:
:HDX-MS has emerged as a powerful tool to interrogate the structure and dynamics of proteins and their complexes. Recent advances in the methodology and instrumentation have enabled the application of HDX-MS to membrane proteins. Such targets are challenging to investigate with conventional strategies. Developing new tools are therefore pertinent for improving our fundamental knowledge of how membrane proteins function in the cell. Importantly, investigating this central class of biomolecules within their native lipid environment remains a challenge but also a key goal ahead. In this short review, we outline recent progresses in dissecting the conformational mechanisms of membrane proteins using HDX-MS. We further describe how the use of computational strategies can aid the interpretation of experimental data and enable visualisation of otherwise intractable membrane protein states. This unique integration of experiments with computations holds significant potential for future applications.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Lau AM,Jia R,Bradshaw RT,Politis Adoi
10.1042/BST20190880subject
Has Abstractpub_date
2020-06-29 00:00:00eissn
0300-5127issn
1470-8752pii
225552pub_type
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