Abstract:
:Secretory immunoglobulin A (SIgA) antibodies directed against the O antigen of lipopolysaccharide (LPS) are the primary determinants of mucosal immunity to gram-negative enteric pathogens. However, the underlying mechanisms by which these antibodies interfere with bacterial colonization and invasion of intestinal epithelial cells are not well understood. In this study, we report that Sal4, a protective, anti-O5-specific monoclonal IgA, is a potent inhibitor of Salmonella enterica serovar Typhimurium flagellum-based motility. Using video light microscopy, we observed that Sal4 completely and virtually instantaneously "paralyzed" laboratory and clinical strains of serovar Typhimurium. Sal4-mediated motility arrest preceded and occurred independently of agglutination. Polyclonal anti-LPS IgG antibodies and F(ab)(2) fragments were as potent as was Sal4 at impeding bacterial motility, whereas monovalent Fab fragments were 5- to 10-fold less effective. To determine whether motility arrest can fully account for Sal4's protective capacity in vitro, we performed epithelial cell infection assays in which the requirement for flagellar motility in adherence and invasion was bypassed by centrifugation. Under these conditions, Sal4-treated serovar Typhimurium cells remained noninvasive, revealing that the monoclonal IgA, in addition to interfering with motility, has an effect on bacterial uptake into epithelial cells. Sal4 did not, however, inhibit bacterial uptake into mouse macrophages, indicating that the antibody interferes specifically with Salmonella pathogenicity island 1 (SPI-1)-dependent, but not SPI-1-independent, entry into host cells. These results reveal a previously unrecognized capacity of SIgA to "disarm" microbial pathogens on mucosal surfaces and prevent colonization and invasion of the intestinal epithelium.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Forbes SJ,Eschmann M,Mantis NJdoi
10.1128/IAI.00416-08subject
Has Abstractpub_date
2008-09-01 00:00:00pages
4137-44issue
9eissn
0019-9567issn
1098-5522pii
IAI.00416-08journal_volume
76pub_type
杂志文章abstract::Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. Protein profiles of the C. pneumoniae isolates appeared to be the same and were distinct from the other Chlamydia spec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.93-97.1990
更新日期:1990-01-01 00:00:00
abstract::Clostridium perfringens enterotoxin caused a 26 to 41% reduction in the rate of oxygen consumption by rat liver mitochondria when various tricarboxylic acid cycle intermediates were used as substrate. However, P/O ratios were unaltered. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.999-1001.1977
更新日期:1977-03-01 00:00:00
abstract::An enterotoxic factor isolated from cultures of Salmonella yielded reproducible results in the suckling mouse model in contrast to other animal models. The enterotoxin appears to possess properties similar to both the heat-stable and heat-labile enterotoxins of Escherichia coli. Preliminary results indicate that the t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.14-22.1975
更新日期:1975-01-01 00:00:00
abstract::By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi geno...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2047-2053.1988
更新日期:1988-08-01 00:00:00
abstract::The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.1.91-97.1972
更新日期:1972-01-01 00:00:00
abstract::No effective therapy exists for Cryptosporidium parvum, a coccidial protozoan parasite that causes severe diarrhea in patients with AIDS. The role of microtubules in parasite invasion of host cells was investigated by incubating 10(7) oocysts with a HT 29.74 cell line for 24 h in the presence of microtubule-disrupting...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4888-4890.1993
更新日期:1993-11-01 00:00:00
abstract::Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2115-2120.2006
更新日期:2006-04-01 00:00:00
abstract::Intravenous injection of rat anti-mouse gamma interferon (IFN-gamma) monoclonal antibody as well as rabbit anti-mouse tumor necrosis factor (TNF) antibody into mice which had received a sublethal infection with Listeria monocytogenes cells resulted in acceleration of listeriosis. Endogenous IFN-gamma seemed to be prod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3331-3337.1989
更新日期:1989-11-01 00:00:00
abstract::We previously reported that infection by Mycobacterium tuberculosis, the causative agent of tuberculosis, leads to secretion of alpha/beta interferon (IFN-alpha/beta). While IFN-alpha/beta ordinarily stimulates formation of signal transducer and stimulator of transcription-1 (STAT-1) homodimers and IFN-stimulated gene...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2487-2497.2003
更新日期:2003-05-01 00:00:00
abstract::The immune response of mice to live, heat-killed, or autoclaved Mycobacterium leprae was investigated. After sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. A selected number of these mice were infected with 5 X 10(3) M. leprae, and the re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.79-83.1984
更新日期:1984-01-01 00:00:00
abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2262-2272.2005
更新日期:2005-04-01 00:00:00
abstract::The immunopathology of Chagas' disease was studied in the experimental model of chronic infection in C57BL/10JT or mice. Sublethal infection with Trypanosoma cruzi, Y strain, induced specific antibodies and a delayed hypersensitivity response to parasite antigens. Mice developed chronic chagasic myocarditis but not sk...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3588-3593.1990
更新日期:1990-11-01 00:00:00
abstract::IL12RB1 is a human gene that is important for resistance to Mycobacterium tuberculosis infection. IL12RB1 is expressed by multiple leukocyte lineages, and encodes a type I transmembrane protein (IL12Rβ1) that associates with IL12p40 and promotes the development of host-protective T(H)1 cells. Recently, we observed tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01230-13
更新日期:2015-02-01 00:00:00
abstract::Escherichia coli 83972 is a clinical asymptomatia bacteriuric isolate that is able to colonize the human urinary bladder without inducing an immune response. Here we demonstrate that one of the mechanisms by which this strain has become attenuated is through the mutation of its genes encoding type 1 and P fimbriae. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.781-785.2006
更新日期:2006-01-01 00:00:00
abstract::Sixty-one patients with a variety of different illnesses were studied with respect to skin test anergy and the presence of serum chemotactic inhibitors. In initial testing, 55% of the patient tests demonstrated negative skin test responses to all six test antigens. Sera from 65% of these anergic patients were capable ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1003-1009.1974
更新日期:1974-11-01 00:00:00
abstract::Transposon Tn916 was used to insertionally inactivate a coaggregation-relevant locus of Streptococcus gordonii DL1 (Challis). One mutant (F11) was isolated that lost the ability to coaggregate with the streptococcal partners of DL1 but retained the ability to coaggregate with partners belonging to other genera. A prob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.10.4137-4142.1996
更新日期:1996-10-01 00:00:00
abstract::Human granulocytic anaplasmosis (HGA) is caused by the obligate intracellular bacterium Anaplasma phagocytophilum. The critical role of gamma interferon (IFN-gamma) for induction of severe inflammatory histopathology, even in the absence of a significant bacterial load, was previously demonstrated in a murine model of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00098-07
更新日期:2007-06-01 00:00:00
abstract::Because of the association of the group A streptococcal pyrogenic exotoxins (SPEs) with erythrogenic toxin used in the classical Dick test, the involvement of the SPEs in production of erythematous skin reactions was assessed. Unless they had been presensitized, young adult rabbits failed to show skin reactions after ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.467-472.1979
更新日期:1979-11-01 00:00:00
abstract::With a purified beta-propiolactone-inactivated rabies virus, a significant increase in footpad swelling was elicited in normal or in BCG-pretreated mice after immunization with varying doses of rabies vaccine. These footpad reactions were shown to peak at 24 h and to be associated with an infiltration of newly formed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.931-939.1978
更新日期:1978-09-01 00:00:00
abstract::Campylobacter rectus is a putative periodontopathogen which expresses a proteinaceous surface layer (S-layer) external to the outer membrane. S-layers are considered to play a protective role for the microorganism in hostile environments. The S-layer proteins from six different C. rectus strains (five human isolates a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.478-483.1997
更新日期:1997-02-01 00:00:00
abstract::Cell wall proteins with sialidase activity are involved in carbohydrate assimilation, adhesion to mucosal surfaces, and biofilm formation. Gardnerella spp. inhabit the human vaginal microbiome and encode up to three sialidase enzymes, two of which are suspected to be cell wall associated. Here we demonstrate that the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00583-20
更新日期:2020-12-23 00:00:00
abstract::Dendritic cells (DC) suffer a maturation defect following interaction with erythrocytes infected with malaria parasites and become unable to induce protective malaria liver-stage immunity. Here we show that, by contrast, maturation-arrested DC in vitro are capable of the successful induction of antigen-specific gamma ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5331-5339.2004
更新日期:2004-09-01 00:00:00
abstract::The antihistoplasma activity of recombinant murine gamma interferon (rMuIFN-gamma)-treated macrophages of the RAW 264.7 cell line depends on the generation of nitric oxide (NO.) from L-arginine. Macrophages of the P388D1 cell line treated with rMuIFN-gamma do not produce NO. or inhibit the intracellular growth of Hist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1478-1479.1994
更新日期:1994-04-01 00:00:00
abstract::Acute pyelonephritis, a complication of Escherichia coli bacteriuria, must represent a bacterial invasion through the kidney epithelium. To study this process, we overlaid bacterial suspensions onto monolayers of cultured human kidney proximal tubular epithelial cells and measured cytotoxicity by release of lactate de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1281-1289.1990
更新日期:1990-05-01 00:00:00
abstract::In previous work, we identified xanthine oxidase (XO) as an important enzyme in the interaction between the host and enteropathogenic Escherichia coli(EPEC) and Shiga-toxigenic E. coli(STEC). Many of the biological effects of XO were due to the hydrogen peroxide produced by the enzyme. We wondered, however, if uric ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01389-15
更新日期:2016-03-24 00:00:00
abstract::Ehrlichia chaffeensis, a tick-borne rickettsial organism, causes the disease human monocytic ehrlichiosis. The pathogen also causes disease in several other vertebrates, including dogs and deer. In this study, we assessed two clonally purified E. chaffeensis mutants with insertions within the genes Ech_0379 and Ech_06...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00487-15
更新日期:2015-07-01 00:00:00
abstract::Invasive aspergillosis is a major threat for patients suffering from chronic granulomatous disease (CGD). Although Aspergillus fumigatus is the most commonly encountered Aspergillus species, the presence of A. nidulans appears to be disproportionately high in CGD patients. The purpose of this study was to investigate ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00921-10
更新日期:2011-02-01 00:00:00
abstract::Antibodies induced in sera and saliva of rats and rabbits immunized with ribosomal preparations from Streptococcus mutans 6715 inhibited transport of glucose by the phosphotransferase system by greater than 60%, acid production from sucrose by greater than 95%, and growth of the homologous S. mutans by greater than 59...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.286-289.1984
更新日期:1984-07-01 00:00:00
abstract::Differences in susceptibility to persistent cryptosporidial infection between two strains of adult athymic nude mice prompted us to investigate the immune mechanism(s) that may control resistance to infection in these T-cell-deficient mice. We studied fecal oocyst shedding, serum and fecal parasite-specific antibody r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.3947-3951.1999
更新日期:1999-08-01 00:00:00
abstract::C57BL/6 mice infected with Leishmania mexicana showed depression of the in vitro immunoglobulin M-plaque-forming cell response to sheep erythrocytes. Immunodepression was present 3 weeks after inoculation and was maximal at 11 weeks. Thereafter, there was a gradual return to normal immunoresponsiveness correlated with...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.415-419.1981
更新日期:1981-05-01 00:00:00