Abstract:
:The immune response of mice to live, heat-killed, or autoclaved Mycobacterium leprae was investigated. After sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. A selected number of these mice were infected with 5 X 10(3) M. leprae, and the remaining animals were sacrificed at scheduled intervals for evidence of cell-mediated immunity to the M. leprae cell extract. Data from these and the bacteriological assays showed that all three materials induce cell-mediated immunity and also extend protection against the M. leprae challenge but not against a Listeria monocytogenes challenge. Adoptive immunity against M. leprae was expressed equally effectively in both non-irradiated animals and those sublethally (500 R) irradiated. This study reveals that, after adoptive transfer of immunity, a bacillary restriction occurs with concomitant onset of delayed hypersensitivity and that the protection observed could be specifically directed against an M. leprae challenge.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Graham L Jr,Navalkar RGdoi
10.1128/IAI.43.1.79-83.1984subject
Has Abstractpub_date
1984-01-01 00:00:00pages
79-83issue
1eissn
0019-9567issn
1098-5522journal_volume
43pub_type
杂志文章abstract::A major problem in the study of chlamydial genital infections in animal models has been the use of varied doses of chlamydiae for infection in different laboratories. It is clearly desirable to use a dose which approximates that of natural sexual infection, but that dose to date has not been determined because of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6148-6154.2003
更新日期:2003-11-01 00:00:00
abstract::The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.1.91-97.1972
更新日期:1972-01-01 00:00:00
abstract::Human and rat C-reactive proteins, major acute-phase reactants, bind to sporozoites and inhibit their in vitro development in hepatocytes (A. Nussler, S. Pied, M. Pontet, F. Miltgen, L. Renia, M. Gentilini, and D. Mazier, Exp. Parasitol. 72:1-7, 1991, and S. Pied, A. Nussler, M. Pontet, F. Miltgen, H. Matile, P.-H. La...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.348-349.1993
更新日期:1993-01-01 00:00:00
abstract::Infection with highly pathogenic Streptococcus suis can cause septic shock, which is characterized by high levels of inflammatory cytokines and a high mortality rate. Our previous study indicated that TREM-1 (triggering receptor expressed on myeloid cells 1) was upregulated in swine spleen cells in response to S. suis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00440-15
更新日期:2015-08-01 00:00:00
abstract::The gonococcal pilus is a primary virulence factor, providing the initial attachment of the bacterial cell to human mucosal tissues. Pilin, the major subunit of the pilus, can carry a wide spectrum of primary amino acid sequences which are generated by the action of a complex antigenic variation system. Changes in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1918-1927.1998
更新日期:1998-05-01 00:00:00
abstract::We developed a porcine dermal explant model to determine the extent to which Staphylococcus aureus biofilm communities deplete oxygen, change pH, and produce damage in underlying tissue. Microelectrode measurements demonstrated that dissolved oxygen (DO) in biofilm-free dermal tissue was 4.45 ± 1.17 mg/liter, while DO...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.03075-14
更新日期:2015-06-01 00:00:00
abstract::Immunity to pneumococcal colonization in mice by exposure to live or killed pneumococci has been shown to be antibody independent but dependent on CD4+ T cells. Here we show that intranasal immunization with pneumococcal proteins (pneumococcal surface protein C, adhesin A, and a pneumolysoid) can elicit a similar mech...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00773-07
更新日期:2007-11-01 00:00:00
abstract::Previous studies have suggested that interleukin-4 (IL-4) has a protective effect in host defense to Borrelia burgdorferi infection, both in limiting the severity of arthritis and in controlling spirochete numbers in tissues, and a mapping study revealed suggestive linkage to a cluster of genes on mouse chromosome 11,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5603-5609.2000
更新日期:2000-10-01 00:00:00
abstract::Cryptococcus neoformans is surrounded by a capsular polysaccharide. There are at least four known serotypes of the polysaccharide. The objective of this study was to produce monoclonal antibodies (MAbs) that could be used to study the distribution of epitopes among the serotypes of C. neoformans. BALB/c mice were immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1895-1899.1987
更新日期:1987-08-01 00:00:00
abstract::The genes of the lic1 operon (lic1A to lic1D) are responsible for incorporation of phosphocholine (PCho) into the lipopolysaccharide (LPS) of Haemophilus influenzae. PCho plays a multifaceted role in the commensal and pathogenic lifestyles of a range of mucosal pathogens, including H. influenzae. Structural studies of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00748-07
更新日期:2008-02-01 00:00:00
abstract::Human systemic antibody levels to oral members of the Bacteroides genus were assessed with an enzyme-linked immunosorbent assay. Antibody levels to B. gingivalis, two homology groups of B. intermedius, B. melaninogenicus, B. denticola, B. loescheii, B. corporis, B. oralis, B. buccae, and B. gracilis were determined in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.2.507-513.1986
更新日期:1986-02-01 00:00:00
abstract::The Salmonella dublin virulence plasmid mediates systemic infection in mice and cattle. Here, we analyze the interaction between wild-type and plasmid-cured Salmonella strains with phagocytes in vitro and in vivo. The intracellular recovery of S. dublin from murine peritoneal and bovine alveolar macrophages cultured i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3385-3393.1996
更新日期:1996-08-01 00:00:00
abstract::We have demonstrated that possession of the gene for thermostable direct hemolysin-related hemolysin (trh) coincides with the presence of the urease gene among clinical Vibrio parahaemolyticus strains and that the location of the two genes are in close proximity on the chromosome. Here, we cloned and sequenced the 15,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5742-5748.2000
更新日期:2000-10-01 00:00:00
abstract::C4BP (C4b-binding protein) is a high-molecular-weight plasma protein that inhibits the classical pathway of complement activation. Recent experiments have demonstrated that C4BP binds to many strains of the gram-positive bacterium Streptococcus pyogenes, a major respiratory tract pathogen. Binding to S. pyogenes was s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3638-3643.1997
更新日期:1997-09-01 00:00:00
abstract::A plaque assay system for pathogenic rickettsiae, which utilizes primary chick embryo tissue cultures, is described. It proved to be a highly reproducible measure of infectiousness for Rickettsia rickettsi and R. typhi, which were employed in most studies; as well as for R. canada, R. prowazeki, R. sibirica, R. akari,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.5.715-722.1972
更新日期:1972-05-01 00:00:00
abstract::The interaction of the first component of complement with two serum-sensitive strains of Escherichia coli and Klebsiella pneumoniae was studied. It could be demonstrated that highly purified C1, free of immunoglobulin G and immunoglobulin M, binds to E. coli or K. pneumoniae. C1 binding was also found with specificall...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.1.5-9.1978
更新日期:1978-10-01 00:00:00
abstract::Capsular types 5 and 8, which account for about 70% of Staphylococcus aureus strains isolated from the blood of patients, resisted in vitro phagocytosis by human polymorphonuclear leukocytes (PMN). Antisera and monoclonal antibody to type 5 and 8 capsular polysaccharides (CPS) induced type-specific in vitro phagocytos...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1090-1095.1988
更新日期:1988-05-01 00:00:00
abstract::Legionella pneumophila is a bacterial pathogen that thrives in alveolar macrophages, causing a severe pneumonia. The virulence of L. pneumophila depends on its Dot/Icm type IV secretion system (T4SS), which delivers more than 300 effector proteins into the host, where they rewire cellular signaling to establish a repl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00785-15
更新日期:2015-10-01 00:00:00
abstract::We previously reported that two surface-exposed high-molecular-weight proteins, HMW1 and HMW2, expressed by a prototypic strain of nontypeable Haemophilus influenzae (NTHI) mediate attachment to human epithelial cells. These proteins are members of a family of highly immunogenic proteins common to most nontypeable Hae...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3032-3037.1996
更新日期:1996-08-01 00:00:00
abstract::The properties of two candidate Salmonella typhi-based live oral typhoid vaccine strains, BRD691 (S. typhi Ty2 harboring mutations in aroA and aroC) and BRD1116 (S. typhi Ty2 harboring mutations in aroA, aroC, and htrA), were compared in a number of in vitro and in vivo assays. BRD1116 exhibited an increased susceptib...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.700-707.1999
更新日期:1999-02-01 00:00:00
abstract::FcRgamma and interleukin-10 (IL-10) are both required for chronic disease in C57BL/6 mice with Leishmania mexicana parasite infection. FcRgamma is a component of several different FcRs and may be a component of some T-cell receptors. The initial antibody response to L. mexicana is an immunoglobulin G1 (IgG1) response,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-07
更新日期:2008-02-01 00:00:00
abstract::Ehrlichia chaffeensis, an obligatory intracellular bacterium of monocytes or macrophages, is the etiologic agent of human monocytic ehrlichiosis. Our previous study showed that gamma interferon (IFN-gamma) added prior to or at early stage of infection inhibited infection of human monocytes with E. chaffeensis; however...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2514-2520.1998
更新日期:1998-06-01 00:00:00
abstract::Helicobacter pylori vacuolating cytotoxin (VacA) is a secreted protein that induces vacuolation of epithelial cells. To study VacA structure and function, we immunized mice with purified type s1-m1 VacA from H. pylori strain 60190 and generated a panel of 10 immunoglobulin G1kappa anti-VacA monoclonal antibodies. All ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4329-4336.2001
更新日期:2001-07-01 00:00:00
abstract::The skin microenvironment at the site of infection plays a role in the early events that determine protective T helper 1/type 1 immune responses during cutaneous leishmaniasis (CL) infection. During CL in nonhealing BALB/c mice, early interleukin-4 (IL-4) can instruct dendritic cells for protective Th1 immunity. Addit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00710-18
更新日期:2018-11-20 00:00:00
abstract::The Lyme disease spirochete Borrelia burgdorferi reduces the expression of outer surface protein C (OspC) in response to the development of an anti-OspC humoral response, leading to the hypothesis that the ability to repress OspC expression is critical for the pathogen to proceed to chronic infection. B. burgdorferi w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00713-06
更新日期:2006-09-01 00:00:00
abstract::The proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha) induces lethal hepatitis when injected into D-(+)-galactosamine-sensitized mice on the one hand or systemic inflammatory response syndrome (SIRS) in normal mice on the other hand. We studied whether serum amyloid P component (SAP), the major acute-ph...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5026-5029.2000
更新日期:2000-09-01 00:00:00
abstract::We wished to find a simple, biologically relevant method to evaluate the virulence of dengue viruses for human beings. Since cells of mononuclear phagocyte lineage may be important sites of dengue infection in primates, we evaluated the permissiveness of these cells to dengue virus as a correlate of virus virulence. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.102-106.1981
更新日期:1981-01-01 00:00:00
abstract::Merozoite surface protein 2 (MSP2) is an abundant glycosylphosphatidylinositol (GPI)-anchored protein of Plasmodium falciparum, which is a potential component of a malaria vaccine. As all forms of MSP2 can be categorized into two allelic families, a vaccine containing two representative forms of MSP2 may overcome the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00665-12
更新日期:2012-12-01 00:00:00
abstract::Pneumonic plague, caused by inhalation of Yersinia pestis, represents a major bioterrorism threat for which no vaccine is available. Based on the knowledge that genetic delivery of monoclonal antibodies (MAbs) with adenovirus (Ad) gene transfer vectors results in rapid, high-level antibody expression, we evaluated the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00856-08
更新日期:2009-04-01 00:00:00
abstract::Fimbriae are colonization factors of the human pathogen Haemophilus influenzae in that they mediate bacterial adherence to human eukaryotic cells. The contribution of the major (HifA) and putative minor (HifD and HifE) subunits of H. influenzae fimbriae to fimbria-specific adherence was studied by using mutants that w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4883-4889.1995
更新日期:1995-12-01 00:00:00