Abstract:
:The properties of two candidate Salmonella typhi-based live oral typhoid vaccine strains, BRD691 (S. typhi Ty2 harboring mutations in aroA and aroC) and BRD1116 (S. typhi Ty2 harboring mutations in aroA, aroC, and htrA), were compared in a number of in vitro and in vivo assays. BRD1116 exhibited an increased susceptibility to oxidative stress compared with BRD691, but both strains were equally resistant to heat shock. Both strains showed a similar ability to invade Caco-2 and HT-29 epithelial cells and U937 macrophage-like cells, but BRD1116 was less efficient at surviving in epithelial cells than BRD691. BRD1116 and BRD691 were equally susceptible to intracellular killing within U937 cells. Similar findings were demonstrated in vivo, with BRD1116 being less able to survive and translocate to secondary sites of infection when inoculated into the lumen of human intestinal xenografts in SCID mice. However, translocation of BRD1116 to spleens and livers in SCID mice occurred as efficiently as that of BRD691 when inoculated intraperitonally. The ability of BRD1116 to increase the secretion of interleukin-8 following infection of HT-29 epithelial cells was comparable to that of BRD691. Therefore, loss of the HtrA protease in S. typhi does not seem to alter its ability to invade epithelial cells or macrophages or to induce proinflammatory cytokines such as IL-8 but significantly reduces intracellular survival in human intestinal epithelial cells in vitro and in vivo.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lowe DC,Savidge TC,Pickard D,Eckmann L,Kagnoff MF,Dougan G,Chatfield SNdoi
10.1128/IAI.67.2.700-707.1999subject
Has Abstractpub_date
1999-02-01 00:00:00pages
700-7issue
2eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::In experimentally infected human volunteers, the cutaneous immune response to Haemophilus ducreyi is orchestrated by serum, polymorphonuclear leukocytes, macrophages, T cells, and myeloid dendritic cells (DC). This response either leads to spontaneous resolution of infection or progresses to pustule formation, which i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00777-07
更新日期:2007-12-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa is a major cause of sepsis in severely burned patients. If it is not eradicated from the wound, it translocates to the bloodstream, causing sepsis, multiorgan failure, and death. We recently described the P. aeruginosa heparinase-encoding gene, hepP, whose expression w...
journal_title:Infection and immunity
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doi:10.1128/IAI.00755-17
更新日期:2017-12-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2767-2773.1991
更新日期:1991-08-01 00:00:00
abstract::Toxigenic Vibrio cholerae strains are lysogens of CTXPhi, a filamentous phage which encodes cholera toxin. The receptor for CTXPhi for invading V. cholerae cells is the toxin-coregulated pilus (TCP), the genes for which reside in a larger genetic element, the TCP pathogenicity island. We analyzed 146 CTX-negative stra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5819-5825.1998
更新日期:1998-12-01 00:00:00
abstract::Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of delivery, and epithelial cells were dissociated from the amniotic membranes by trypsinization and grown in Rose chambers. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2042-2047.1990
更新日期:1990-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00850-16
更新日期:2017-03-23 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2012-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.776-781.1985
更新日期:1985-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00232-08
更新日期:2008-07-01 00:00:00
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更新日期:2009-10-01 00:00:00
abstract::Bovine septicemic Escherichia coli 31A agglutinates bovine, rabbit, and human erythrocytes and adheres in vitro to the brush border of bovine or ovine intestinal epithelial cells and to the human colon carcinoma Caco-2 cell line. The adhesion and hemagglutination of E. coli 31A are mediated by a chromosome-encoded fim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.332-342.1996
更新日期:1996-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.181-187.1979
更新日期:1979-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.392-400.1992
更新日期:1992-02-01 00:00:00
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doi:10.1128/IAI.61.6.2698-2702.1993
更新日期:1993-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.54-58.1984
更新日期:1984-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2002-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1989-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.7.6.937-940.1973
更新日期:1973-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4005-4014.1994
更新日期:1994-09-01 00:00:00
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更新日期:2000-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2564-2569.1995
更新日期:1995-07-01 00:00:00
abstract::While several diseases associated with Chlamydia psittaci infection have been reported in Phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. In this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strai...
journal_title:Infection and immunity
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更新日期:1988-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5231-5242.1999
更新日期:1999-10-01 00:00:00
abstract::Previously, we isolated a high-molecular-weight immunogenic polysaccharide (designated PS) from Pseudomonas aeruginosa immunotype 1 (IT-1). The method which we used was modified to permit the isolation of a similar PS from P. aeruginosa IT-2. This antigen was composed primarily of carbohydrate, had a complex monosacch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.461-468.1981
更新日期:1981-11-01 00:00:00
abstract::The gene encoding Clostridium sordellii phospholipase C (Csp) was cloned and expressed as a histidine-tagged (His-tag) protein, and the protein was purified to compare its enzymatic and biological activities with those of Clostridium perfringens phospholipase C (Cpa) and Clostridium bifermentans phospholipase C (Cbp)....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.641-646.2003
更新日期:2003-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-19
更新日期:2019-08-21 00:00:00
abstract::Pasteurella multocida is the causative agent of a number of diseases in animals, including fowl cholera. P. multocida strains simultaneously express two lipopolysaccharide (LPS) glycoforms (glycoforms A and B) that differ only in their inner core structure. Glycoform A contains a single 3-deoxy-d-manno-octulosonic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00457-10
更新日期:2010-09-01 00:00:00
abstract::The protective immunity conferred by T-cell subsets against infection with Treponema pallidum subsp. pertenue was studied. We demonstrated that hamster T cells can be separated into two subsets by monoclonal antibody (MAb) GK 1.5 (anti-L3T4) and MAb 38. Eighty-five percent of hamster thymocytes were L3T4+ and 87% were...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.2.529-536.1991
更新日期:1991-02-01 00:00:00