Abstract:
:Incubation of large granular lymphocytes (LGL) with glutaraldehyde-fixed bacteria stimulated in the supernatant the production of interferon (IFN), which proved to be mainly IFN-gamma. Even though IFN-gamma was produced upon exposure of LGL to bacteria, anti-IFN-gamma antibodies failed to interfere with induction of cytotoxicity by bacterial contact. Anti-IFN-gamma receptor antibodies had no effect on the induction of activated killing by bacterial contact either. We also tested the effect of anti-IFN-alpha antibody, but it failed to interfere with induction of cytotoxicity by bacterial contact. No interleukin-2 (IL-2) was detected in the culture supernatant of bacterially activated LGL by the mouse HT2 cell assay, nor did we detect any IL-2 mRNA in bacterially activated LGL by Northern RNA blot assay. Neutralizing anti-IL-2 antiserum had no effect on the induction of activated killing by bacterial contact, and recombinant IL-4 did not interfere with the induction of activated killing. We then studied the membrane structures involved in bacterially activated killing. Anti-CD18 monoclonal antibody did not interfere with the induction phase of bacterially activated killing. However, both anti-CD18 and anti-CD2 antibodies inhibited the effector phase of bacterially activated killing. The effector pathways utilized by activated LGL depended on the mode of activation in that even though bacterially activated LGL were sometimes blocked by anti-CD2 monoclonal antibody, recombinant-IL-2-stimulated LGL were not. In conclusion, our present results suggest that there may be mediators other than exogenously secreted IFNs and IL-2 which are responsible for the induction of activated killing after bacterial contact. CD18 and CD2 structures were shown to be involved in the effector phase of bacterially activated killing.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tarkkanen J,Saksela Edoi
10.1128/IAI.59.8.2767-2773.1991subject
Has Abstractpub_date
1991-08-01 00:00:00pages
2767-73issue
8eissn
0019-9567issn
1098-5522journal_volume
59pub_type
杂志文章abstract::Nasopharyngeal colonization is a necessary first step in the pathogenesis of Streptococcus pneumoniae. Using isolates containing defined mutations in the S. pneumoniae capsule locus, we found that expression of the capsular polysaccharide is essential for colonization by the type 2 strain D39 and the type 3 strains A6...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3755-3761.2001
更新日期:2001-06-01 00:00:00
abstract::Peritoneal exudate T lymphocytes from Listeria monocytogenes-immune mice in the presence of the homologous antigen (heat-killed L. monocytogenes) and normal macrophages showed L. monocytogenes-specific proliferative responses. Proliferation was inhibited by macrophages from L. monocytogenes- or Corynebacterium parvum-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.907-913.1982
更新日期:1982-12-01 00:00:00
abstract::Slime production is not a generally recognized feature of Staphylococcus epidermidis. In a recent outbreak of S. epidermidis intravascular catheter-associated sepsis, we noted that 63% of clinically implicated strains grew as a slimy film coating the culture tube walls when propagated in tryptic soy broth. Only 37% of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.318-326.1982
更新日期:1982-07-01 00:00:00
abstract::Most Helicobacter pylori strains express the BabA adhesin, which binds to ABO/Leb blood group antigens on gastric mucin and epithelial cells and is found more commonly in strains that cause peptic ulcers or gastric cancer, rather than asymptomatic infection. We and others have previously reported that in mice, gerbils...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00094-17
更新日期:2017-05-23 00:00:00
abstract::Helicobacter pylori maintains colonization in its human host using a limited set of taxis sensors. TlpD is a proposed energy taxis sensor of H. pylori and dominant under environmental conditions of low bacterial energy yield. We studied the impact of H. pylori TlpD on colonization in vivo using a gerbil infection mode...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00750-13
更新日期:2013-10-01 00:00:00
abstract::Human colostrum, parotid saliva, and serum were assayed for the presence of naturally occurring antibodies to five serotypes of Streptococcus mutans. Appreciable levels of agglutinins to strains AHT, BHT, 10449, 6715, and LM-7 (groups a leads to e, respectively) were detected in normal colostrum and saliva, whereas re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.355-362.1976
更新日期:1976-08-01 00:00:00
abstract::Since melanin is considered a virulence factor in Cryptococcus neoformans, its suppression at 37 degrees C has been perplexing. We now show an opposite thermal regulation of superoxide dismutase (SOD), consistent with a compensatory mechanism. Moreover, we demonstrate normal SOD and catalase levels in albino, oxygen-s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4085-4086.1994
更新日期:1994-09-01 00:00:00
abstract::Vibrio cholerae causes human infection through ingestion of contaminated food and water, leading to the devastating diarrheal disease cholera. V. cholerae forms matrix-encased aggregates, known as biofilms, in the native aquatic environment. While the formation of V. cholerae biofilms has been well studied, little is ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02617-14
更新日期:2015-01-01 00:00:00
abstract::The ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine WEHI 231 cells, splenic B cells, and human RAJI cells was examined. The culture filtrate of Porphyromonas gingivalis, Prevotella loescheii, and Fusobacterium nucleatum, which contains high a percentag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2587-2594.1998
更新日期:1998-06-01 00:00:00
abstract::BCG vaccines are a family of closely related daughter strains of an attenuated isolate of Mycobacterium bovis derived by in vitro passage from 1908 to 1921. During subsequent laboratory propagation of the vaccine strain until its lyophilization in 1961, BCG Pasteur underwent at least seven further genomic mutations. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2803-2809.2004
更新日期:2004-05-01 00:00:00
abstract::Mycoplasma penetrans adhered to cultured human cells, forming clusters that localized to specific areas of the host cell surface. Adherence and cluster formation were inhibited by anti-M. penetrans antibodies, suggesting the involvement of specific adhesin-receptor interactions. Ultrastructural studies showed that aft...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.197-208.1996
更新日期:1996-01-01 00:00:00
abstract::In the absence of antiserum, rabbit polymorphonuclear leukocytes (PMNs) released lysosomal enzymes in response to Actinomyces viscosus (19246) but not to Streptococcus mutans (6715). Antibodies had a marked modulating influence on these reactions. PMN hydrolase release was significantly enhanced to both organisms when...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.6.1315-1321.1976
更新日期:1976-12-01 00:00:00
abstract::Isolates of Thermoplasma acidophilum were divided into serological groups based on immunodiffusion studies of solubilized-membrane preparations. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.280-281.1974
更新日期:1974-07-01 00:00:00
abstract::Recent reports have demonstrated that Bordetella pertussis has invasive behavior in vivo and in vitro. In this study, we investigated the ability of a virulent strain, avirulent mutants, and mutants deficient in specific virulence factors to enter and survive intracellularly in human macrophages in vitro. Uptake of vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4578-4585.1992
更新日期:1992-11-01 00:00:00
abstract::Woodchuck sera were tested for antigens and antibodies with tests which detect human hepatitis virus antigens and antibodies. Data on 264 woodchuck sera are presented. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.752-757.1982
更新日期:1982-02-01 00:00:00
abstract::Immunization of BALB/c mice with a plasmid containing the gene for Trypanosoma cruzi trans-sialidase (TS) induced antibodies that inhibited TS enzymatic activity, CD4+ Th1 and CD8+ Tc1 cells, and protective immunity against infection. We used this model to obtain basic information on the requirement of CD4 or CD8 or B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5477-5486.2001
更新日期:2001-09-01 00:00:00
abstract::In the murine model of urinary tract infections (UTI), cystitis by uropathogenic Escherichia coli (UPEC) occurs through an intimate relationship with the bladder superficial umbrella cell entailing cycles of adherence, invasion, intracellular bacterial community (IBC) formation, and dispersal (fluxing) from the intrac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7657-7668.2005
更新日期:2005-11-01 00:00:00
abstract::Agglutinogen 1 of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen 1 migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.243-248.1971
更新日期:1971-02-01 00:00:00
abstract::Burkholderia pseudomallei is a CDC tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common, and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02785-14
更新日期:2015-05-01 00:00:00
abstract::Group A streptococcal pyrogenic exotoxins types A, B, and C and staphylococcal pyrogenic exotoxin were shown to be potent nonspecific T-lymphocyte mitogens. Adherent cell populations did not significantly affect the nonspecific mitogenicity. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.1075-1077.1979
更新日期:1979-09-01 00:00:00
abstract::Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01010-07
更新日期:2008-02-01 00:00:00
abstract::We developed an in vitro culture method to characterize the expression of bacterial genes under conditions mimicking the colonic environment. Our culture system (the intestinal simulator) comprised a continuous-flow anaerobic culture which was inoculated with fecal samples from healthy volunteers. As a test organism, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.3793-3802.2004
更新日期:2004-07-01 00:00:00
abstract::Porphyromonas gingivalis is a major etiologic agent of periodontitis, a chronic inflammatory disease that ultimately results in the loss of the supporting tissues of the teeth. Previous work has demonstrated the usefulness of avirulent Salmonella enterica serovar Typhimurium strains as antigen delivery systems for pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.732-739.2000
更新日期:2000-02-01 00:00:00
abstract::We have cloned chromosomal genes mediating the aerobactin iron transport system from the enteroinvasive strain Escherichia coli 978-77. The physical map of the region spanning the siderophore biosynthesis genes and the upstream portion of the receptor gene in strain 978-77-derived clones was identical to the correspon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.357-364.1991
更新日期:1991-01-01 00:00:00
abstract::Spleen cells from BALB/c mice infected with 2 X 10(7) L. major promastigotes and developing progressive disease produced significantly lower levels of interleukin-2 (IL-2) in response to concanavalin A stimulation than did spleen cells from uninfected mice. In contrast, spleen cells from sublethally irradiated and inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.386-394.1986
更新日期:1986-11-01 00:00:00
abstract::Antibody levels in sera and respiratory secretions and resistance to respiratory infections were examined in mice given live infuenza virus in small-particle (2 mum) aerosols, large-particle (10 mum) aerosols, intraperitoneally, and subcutaneously. After parenteral administration antibody was found primarily in the se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.696-703.1976
更新日期:1976-03-01 00:00:00
abstract::Host control of Coxiella burnetii infections is believed to be mediated primarily by activated monocytes/macrophages. The activation of macrophages by cytokines leads to the production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) that have potent antimicrobial activities. The contri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6666-6675.2004
更新日期:2004-11-01 00:00:00
abstract::Infection of L cells with Chlamydia psittaci inhibited the burst of deoxyribonucleic acid synthesis that occurs when the host cells are released from a double thymidine block. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.363-364.1971
更新日期:1971-02-01 00:00:00
abstract::Schistosoma mansoni-infected wild-type (WT) mice develop a Th2 response and chronic disease. In contrast, infected interleukin-4 double-deficient (IL-4(-/-)) mice develop a Th1-like response and an acute, lethal syndrome. Disease severity in these animals correlates with excessive and prolonged production of nitric ox...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.177-184.2002
更新日期:2002-01-01 00:00:00
abstract::Otitis media developed in 67% of chinchillas inoculated intranasally with type 7 Streptococcus pneumoniae and influenza A virus. Only 4% of chinchillas inoculated with influenza alone and 21% of chinchillas inoculated with S. pneumoniae alone developed otitis media. Among the chinchillas that developed otitis media af...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00