Abstract:
:Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identity. In this study we investigated the role of PapB and FocB in regulation of the F1C fimbriae. By using gel mobility shift assays, we showed that FocB binds to sequences in both the pap and foc operons in a somewhat different manner than PapB. The results of both in vitro cross-linking and in vivo oligomerization tests indicated that FocB could function in an oligomeric fashion. Furthermore, our results suggest that PapB and FocB can form heterodimers and that these complexes can repress expression of the foc operon. The effect of FocB on expression of type 1 fimbriae was also tested. Taken together, the results that we present expand our knowledge about a regulatory network for different adhesin gene systems in uropathogenic E. coli and suggest a hierarchy for expression of the fimbrial adhesins.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lindberg S,Xia Y,Sondén B,Göransson M,Hacker J,Uhlin BEdoi
10.1128/IAI.01010-07subject
Has Abstractpub_date
2008-02-01 00:00:00pages
771-80issue
2eissn
0019-9567issn
1098-5522pii
IAI.01010-07journal_volume
76pub_type
杂志文章abstract::The hallmarks of enteropathogenic Escherichia coli (EPEC) infection are formation of attaching and effacing (A/E) lesions on mucosal surfaces and actin-rich pedestals on cultured cells, both of which are dependent on the type III secretion system effector Tir. Following translocation into cultured cells and clustering...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-15
更新日期:2015-09-01 00:00:00
abstract::Mice were infected with 170 Trichinella spiralis larvae, and their blood clearance of carbon particles was studied 4, 7, 13, 32, and 135 days later and compared with that of noninfected control mice. Clearance in mice with a 4-day-old infection was comparable to that in the controls; clearances in mice with 7- and 13-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Phenethyl alcohol, at the maximum concentration which did not inhibit growth (0.3% [vol/vol]), inhibited the production of alpha-lysin and exoproteases but not that of delta-lysin in Staphylococcus aureus Wood 46. The inhibition of alpha-lysin was reversible, and transient accumulation of cell-associated alpha-lysin o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.112-117.1985
更新日期:1985-01-01 00:00:00
abstract::We previously identified a cell wall-associated protein from Fusobacterium nucleatum, a Gram-negative bacterium of the oral cavity, that induces human beta defensin 2 (hBD-2) in primary human oral epithelial cells (HOECs) and designated it FAD-I (Fusobacterium-associated defensin inducer). Here, we report differential...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01311-15
更新日期:2016-04-22 00:00:00
abstract::Rabbit-specific enteropathogenic Escherichia coli (REPEC) is an attaching and effacing pathogen of young rabbits. Using signature-tagged mutagenesis, we identified several known colonization factors of REPEC as well as a gene predicted to encode a novel autotransporter protein. This novel gene was termed rpeA for REPE...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00972-06
更新日期:2007-09-01 00:00:00
abstract::Legionella pneumophila organisms are able to infect and multiply within the ciliated protozoan Tetrahymena pyriformis. This ability may be associated with virulence, because an attenuated strain of L. pneumophila fails to multiply within this protozoan, whereas a virulent strain increases 10,000-fold in number when co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.3.553-559.1986
更新日期:1986-09-01 00:00:00
abstract::Lipid X, a precursor in the biosynthesis of lipid A, has been claimed to possess most of the immunostimulatory activity but none of the toxicity of endotoxin. However, recent work shows that synthetic lipid X can be contaminated with small amounts of N,O-acylated disaccharide-1-phosphate (H. Aschauer, A. Grob, J. Hild...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2351-2358.1991
更新日期:1991-07-01 00:00:00
abstract::Protamines (salmine prepared from sperm DNA of salmon and clupeine from herring sperm), which are basic peptides rich in arginine, were found to inhibit the proteolytic activity of arginine-specific cysteine protease (RC-protease) from Porphyromonas gingivalis. Lineweaver-Burk plot analysis revealed that the protamine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4917-4920.1999
更新日期:1999-09-01 00:00:00
abstract::Staphylococcus aureus is an important opportunistic pathogen and is the etiological agent of many hospital- and community-acquired infections. The golden pigment, staphyloxanthin, of S. aureus colonies distinguishes it from other staphylococci and related Gram-positive cocci. Staphyloxanthin is the product of a series...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00838-16
更新日期:2017-01-26 00:00:00
abstract::Comparative studies on the virulence of two strains of Pseudomonas aeruginosa isolated from otic pus and mink pneumonic lesion, respectively, were made by means of intranasal inoculation in mink. The minimal lethal dose of the former (strain NC-5, highly virulent for mice) was 10(10), and that of the latter (strain No...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.16-20.1974
更新日期:1974-07-01 00:00:00
abstract::Our previous studies demonstrated that Mycobacterium bovis bacillus Calmette-Guérin (BCG) can directly interact with human NK cells and induce the proliferation, gamma interferon production, and cytotoxic activity of such cells without the need for accessory cells. Thus, the aim of the present study was to identify th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00870-07
更新日期:2008-04-01 00:00:00
abstract::We investigated the role of bacterial adherence and hemolysin production from Escherichia coli parent and genetically cloned strains as to their effects on histamine release from rat mast cells and leukotriene generation from human polymorphonuclear granulocytes. These mediators were involved in the induction of infla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.271-278.1985
更新日期:1985-10-01 00:00:00
abstract::We have previously shown that Bacillus anthracis lethal toxin represses glucocorticoid receptor (GR) transactivation. We now report that repression of GR activity also occurs with the large clostridial toxins produced by Clostridium sordellii and C. difficile. This was demonstrated using a transient transfection assay...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-07
更新日期:2007-08-01 00:00:00
abstract::Gamma interferon (IFN-gamma) is produced in response to circulating lipopolysaccharide (LPS) and contributes to the lethality of endotoxic shock. To address the cellular source of IFN-gamma production in vivo, T cells and B cells were magnetically purified from C57BL/6 mouse spleens 5 h following endotoxin injection. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4244-4249.1994
更新日期:1994-10-01 00:00:00
abstract::Representative strains of serogroup A Neisseria meningitidis were chosen from all major meningitis epidemics worldwide since 1960 and subjected to analysis for the electrophoretic variation of 15 cytoplasmic allozymes and four outer membrane proteins. The 290 strains defined 84 unique electrophoretic types which were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5267-5282.1992
更新日期:1992-12-01 00:00:00
abstract::Staphylococcal enterotoxins A through D (SEA through SED) and toxic shock syndrome toxin-1 display superantigen properties, i.e., they stimulate a great fraction of T cells expressing certain T-cell receptor V beta sequences. Using a newly established rat model of septic Staphylococcus aureus arthritis, we have recent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.4185-4187.1995
更新日期:1995-10-01 00:00:00
abstract::The ability of hemagglutinating and poorly hemagglutinating strains of the gastroduodenal pathogen Helicobacter pylori to bind 125I-radiolabelled laminin was quantitated in a liquid phase assay. Although all strains bound laminin, some hemagglutinating strains were good binders of laminin (maximum of 31% binding), whe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3640-3648.1994
更新日期:1994-09-01 00:00:00
abstract::Streptolysin-O (SLO) is a thiol-activated, membrane-damaging protein toxin of Mr 69,000 that is produced by most strains of beta-hemolytic group A streptococci. Native, primarily water-soluble toxin molecules bind to cholesterol-containing target membranes to assemble into supramolecular curved rod structures (25 to 1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.52-60.1985
更新日期:1985-01-01 00:00:00
abstract::Diffusely adhering Escherichia coli (DAEC) strains expressing F1845 fimbrial adhesin or Dr hemagglutinin belonging to the Afa/Dr family of adhesins infect cultured polarized human intestinal cells through recognition of the brush border-associated decay-accelerating factor (DAF; CD55) as a receptor. The wild-type Afa/...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5979-5990.2000
更新日期:2000-10-01 00:00:00
abstract::The intravenous administration of hydrogen peroxide has been reported to benefit patients with pneumonia and to reduce Plasmodium parasitemia in experimentally infected mice. We assessed the antibacterial activity of intravenously infused hydrogen peroxide against hydrogen peroxide-susceptible Escherichia coli (MBC of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.607-610.1985
更新日期:1985-06-01 00:00:00
abstract::An analysis of which component of lipopolysaccharide, the lipid or the polysaccharide, is mitogenic for mouse B-lymphocytes has been performed. A purified glycolipid derived from a rough mutant of Salmonella minnesota (R595) that does not contain any o-polysaccharide at all is more mitogenic than an intact lipopolysac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.3.406-411.1973
更新日期:1973-09-01 00:00:00
abstract::Several early nonspecific host defense mechanisms were examined in resistant (BALB/c) and susceptible (C3H/He) mice after intraperitoneal inoculation with Rickettsia tsutsugamushi strain Gilliam. Inflammatory exudates were formed in both mouse strains in response to rickettsial inoculation, but the inflammatory respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1239-1250.1981
更新日期:1981-03-01 00:00:00
abstract::Two distinct and complementary pathways, one mediated by perforin and the other dependent upon CD95 (Fas), effect cell-mediated cytotoxicity. We examined the relative roles of these pathways in host defenses against the intracellular bacterial pathogen Listeria monocytogenes by using murine listeriosis as a model syst...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Production of the Kanagawa hemolysin by patient strains of Vibrio parahaemolyticus was found to respond to the pH rather than to the type of carbohydrate present in the growth medium. Regardless of the carbohydrate present, hemolysin production in peptone broth cultures occurred only when the pH was between 6.5 and 5....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.115-119.1981
更新日期:1981-10-01 00:00:00
abstract::In the lungs of mice vaccinated with Mycobacterium bovis BCG, there was an accumulation of CD4 cells expressing the activated effector phenotype CD44hi CD62 ligandlo) (CD62Llo) which were capable of secreting gamma interferon. Upon cell transfer, however, cells expressing a resting/naïve phenotype (CD44lo CD62Lhi) wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7759-7764.2005
更新日期:2005-11-01 00:00:00
abstract::There is an emerging paradigm shift from coronary heart disease having a purely hereditary and nutritional causation to possibly having an infectious etiology. Recent epidemiological studies have shown a correlation between periodontal disease and coronary heart disease. However, to date, there is minimal information ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5792-5798.1999
更新日期:1999-11-01 00:00:00
abstract::Candida albicans is a ubiquitous mucosal commensal that is normally prevented from causing acute or chronic invasive disease. Neutrophils contribute to protection in oral infection but exacerbate vulvovaginal candidiasis. To dissect the role of neutrophils during mucosal candidiasis, we took advantage of a new, transp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00276-17
更新日期:2017-08-18 00:00:00
abstract::Mutation of purR was previously shown to enhance the virulence of Staphylococcus aureus in a murine sepsis model, and this cannot be fully explained by increased expression of genes within the purine biosynthesis pathway. Rather, the increased production of specific S. aureus virulence factors, including alpha toxin a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00718-20
更新日期:2021-01-19 00:00:00
abstract::Subtilase cytotoxin (SubAB) is a recently identified AB5 subunit toxin produced by Shiga-toxigenic Escherichia coli. The A subunit is thought to be a subtilase-like, serine protease, whereas the B subunit binds to the toxin receptor on the cell surface. We cloned the genes from a clinical isolate; the toxin was produc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01336-06
更新日期:2007-01-01 00:00:00
abstract::Mice were vaccinated with plasmid DNA (pDNA) encoding antigen 85A (Ag85A), Ag85B, or PstS-3 from Mycobacterium tuberculosis either in saline or formulated for intramuscular injections in VC1052:DPyPE (aminopropyl-dimethyl-myristoleyloxy-propanaminium bromide-diphytanoylphosphatidyl-ethanolamine) (Vaxfectin; Vical, Inc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3681-3688.2002
更新日期:2002-07-01 00:00:00