Abstract:
:We have previously shown that Bacillus anthracis lethal toxin represses glucocorticoid receptor (GR) transactivation. We now report that repression of GR activity also occurs with the large clostridial toxins produced by Clostridium sordellii and C. difficile. This was demonstrated using a transient transfection assay system for GR transactivation. We also report that C. sordellii lethal toxin inhibited GR function in an ex vivo assay, where toxin reduced the dexamethasone suppression of the proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha). Furthermore, the glucocorticoid antagonist RU-486 in combination with C. sordellii lethal toxin additively prevented glucocorticoid suppression of TNF-alpha. These findings corroborate the fact that GR is a target for the toxin and suggest a physiological role for toxin-associated GR repression in inflammation. Finally, we show that this repression is associated with toxins that inactivate p38 mitogen-activated protein kinase (MAPK).
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tait AS,Dalton M,Geny B,D'Agnillo F,Popoff MR,Sternberg EMdoi
10.1128/IAI.00291-07subject
Has Abstractpub_date
2007-08-01 00:00:00pages
3935-40issue
8eissn
0019-9567issn
1098-5522pii
IAI.00291-07journal_volume
75pub_type
杂志文章abstract::Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.945-952.2002
更新日期:2002-02-01 00:00:00
abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal pneumonia, sepsis, and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes 35 proteins containing an LPXTG motif which are thought to be covalently linked to the peptidoglycan by an enzyme called sorta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3342-3350.2005
更新日期:2005-06-01 00:00:00
abstract::Alveolar bone resorption can be induced in specific-pathogen-free mice by oral infection with Porphyromonas gingivalis (P. J. Baker, R. T. Evans, and D. C. Roopenian, Arch. Oral Biol. 39:1035-1040, 1994). Here we used a mouse strain, C57BL/6J, which is relatively resistant to P. gingivalis-induced bone loss to examine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3103-3107.2000
更新日期:2000-06-01 00:00:00
abstract::We isolated a monoclonal antibody directed against the ferric complex of aerobactin purified from Escherichia coli KH576. This antibody, which we designated MAb AERO1, was identified as an immunoglobulin G, subtype 2. A competitive enzyme-linked immunosorbent assay with MAb AERO1 had a limit of 10 nM for the detection...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.768-772.1992
更新日期:1992-03-01 00:00:00
abstract::We examined the effect of host age and health status on the adherence of mannose-sensitive piliated Escherichia coli K1 to human oral epithelial cells. Mannose-sensitive piliated bacteria adhered in comparable numbers to newborn, older infant, and adult cells (125 +/- 61, 198 +/- 54, and 139 +/- 69 bacteria per cell, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.299-301.1984
更新日期:1984-07-01 00:00:00
abstract::The antiinflammatory cytokine response during urosepsis was determined by measurement of concentrations of soluble tumor necrosis factor receptor (sTNFR) types I and II, interleukin 1 receptor antagonist (IL-1ra), soluble IL-1 receptor type II (sIL-1RII), and interleukin 10 in sera and urine of 30 patients with cultur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3527-3534.1998
更新日期:1998-08-01 00:00:00
abstract::Listeria monocytogenes colonized the gastrointestinal tract of adult germfree rats (10(10) to 10(11)/g, dry weight) within 24 h after oral exposure. Between 3 and 14 days after monoassociation, L. monocytogenes caused a self-limiting pseudomembranous colitis, bacteremia, and infection of the spleen and liver. Monoasso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.323-331.1981
更新日期:1981-04-01 00:00:00
abstract::The acute phase of Chagas' disease is accompanied by immunosuppression. To explore the underlying mechanism(s), we used an in vitro culture system in which the capacities of activated human peripheral blood mononuclear cells to express interleukin-2 receptors (IL-2R) and proliferate are markedly inhibited in the prese...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.8.2301-2305.1989
更新日期:1989-08-01 00:00:00
abstract::Binding of Escherichia coli strain 431 heat-stable enterotoxin (STa) and activation of intestinal particulate guanylate cyclase by E. coli STa were studied with rat intestinal epithelial cells and brush border membranes (BBMs). The rates of guanylate cyclase stimulation by 431 STa in cells and BBMs were rapid, with ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.537-543.1984
更新日期:1984-11-01 00:00:00
abstract::Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B. anthracis strain was constructed by integrating into the chromosome a translational fusion harboring the DNA fragments ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4847-4850.1999
更新日期:1999-09-01 00:00:00
abstract::Infection of mice with the murine sarcoma virus (Moloney) markedly suppressed the humoral antibody response to sheep erythrocyte antigen injected 10 days after infection, when tumor size was maximal, and on day 26, when primary tumors had partially regressed. Humoral antibody response was also inhibited when antigen w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-03-01 00:00:00
abstract::Attenuated Yersinia pestis pgm strains, such as KIM5, lack the siderophore yersiniabactin. Strain KIM5 does not induce significant pneumonia when delivered intranasally. In this study, mice were found to develop pneumonia after intranasal challenge with strain KIM5 when they were injected intraperitoneally with iron d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00382-10
更新日期:2010-08-01 00:00:00
abstract::Nonimmune binding of immunoglobulin to whole bacteria was quantitated for North American isolates of Haemophilus somnus recovered from cattle with pneumonia, reproductive failure (abortion), or thromboembolic meningoencephalitis or from the vagina or prepuce of carrier cattle. Quantitative binding activity covered a w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.639-642.1989
更新日期:1989-02-01 00:00:00
abstract::Nasopharyngeal colonization is a necessary first step in the pathogenesis of Streptococcus pneumoniae. Using isolates containing defined mutations in the S. pneumoniae capsule locus, we found that expression of the capsular polysaccharide is essential for colonization by the type 2 strain D39 and the type 3 strains A6...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3755-3761.2001
更新日期:2001-06-01 00:00:00
abstract::Recent reports have suggested that oral vaccination of mice against Helicobacter pylori is dependent on a Th1-mediated immune response. However, oral vaccination in mice neither induces sterilizing immunity nor leads to complete protection from disease. Therefore, in this study we investigated whether a systemic subcu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.1029-1035.2004
更新日期:2004-02-01 00:00:00
abstract::Periodontitis is a disease of polymicrobial etiology characterized by inflammation, degradation of host tissue, and bone that irreversibly destroys the supporting apparatus of teeth. Porphyromonas gingivalis contains lipid A with structural heterogeneity that has been postulated to contribute to the initiation of dysb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01136-13
更新日期:2014-02-01 00:00:00
abstract::Human sera were examined by an enzyme-linked immunosorbent assay for immunoglobulin G (IgG) and IgM antibodies to purified type III polysaccharide of group B streptococci. The antigen-binding capacity of a reference human serum was determined by a radioimmunoassay, and the total antibody content was determined by quan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.98-104.1984
更新日期:1984-10-01 00:00:00
abstract::Adhesion of Trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. However, the specific receptors on the host cell have not yet been identified. In this work, the ability of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4358-4360.2000
更新日期:2000-07-01 00:00:00
abstract::Eperythrozoon coccoides is a common blood parasite of rodents and the etiological agent of a chronic infection present in many mouse colonies. After primary infection, mice develop a parasitemia and anemia followed by a chronic, latent infection. During the acute phase of infection, mice manifest a striking suppressio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.4.425-430.1971
更新日期:1971-10-01 00:00:00
abstract::Acute pyelonephritis, a complication of Escherichia coli bacteriuria, must represent a bacterial invasion through the kidney epithelium. To study this process, we overlaid bacterial suspensions onto monolayers of cultured human kidney proximal tubular epithelial cells and measured cytotoxicity by release of lactate de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1281-1289.1990
更新日期:1990-05-01 00:00:00
abstract::Ocular exposure of mice to live elementary bodies of Chlamydia trachomatis serovar A results in immunological sensitization of the mice. This reactivity is manifested by the development of early (5 h) and delayed-type (24 h) dermal reactivity and serovar-specific antibody formation against either live or irradiated (1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.9-12.1986
更新日期:1986-10-01 00:00:00
abstract::The influences of Neisseria gonorrhoeae protein IB content and bilayer composition of liposomes and protein content of iscoms on immunogenicity were investigated. Changes in the protein content of liposomes did not influence the immunoglobulin G response, whereas the response was lowered when the amount of protein in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1661-1664.1988
更新日期:1988-06-01 00:00:00
abstract::We investigated the effects of peripheral blood mononuclear cells expanded with irrelevant control and mycobacterial antigens on the intracellular growth of Mycobacterium bovis bacillus Calmette-Guérin (BCG) in human macrophages. More than 90% of the cells present after 1 week of in vitro expansion were CD3(+). T cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.1763-1773.2003
更新日期:2003-04-01 00:00:00
abstract::Sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of proteins and externally exposed labeled surface constituents were analyzed in promastigotes of three etiological agents of kala azar (Leishmania donovani, HS70 strain from India; L. chagasi, Imperatriz strain from Brazil; L. infantum, ITMPA K263 str...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.136-141.1985
更新日期:1985-10-01 00:00:00
abstract::Changes in the structural components of the Streptococcus pyogenes membrane between exponential and early stationary phases of growth are reported. The overall protein composition ranged from 70 to 73% of the dry weight of the membranes, irrespective of the phase of growth from which they were isolated. Amino acid ana...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.883-891.1979
更新日期:1979-12-01 00:00:00
abstract::The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera from adult volunteers injected with polyvalent pneumococcal vaccines containing either 50 mug of type 6A (U.S. designatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1116-1122.1979
更新日期:1979-12-01 00:00:00
abstract::The effects of infection with Rowson-Parr virus (RPV), an associated virus present in Friend virus (FV) preparations, on the splenic plaque-forming cell response to sheep red cells in adult mice and on the plaque-forming cell response of peritoneal cells in vitro have been investigated. The results support the hypothe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.2.160-166.1973
更新日期:1973-02-01 00:00:00
abstract::The immune response of mice to live, heat-killed, or autoclaved Mycobacterium leprae was investigated. After sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. A selected number of these mice were infected with 5 X 10(3) M. leprae, and the re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.79-83.1984
更新日期:1984-01-01 00:00:00
abstract::An immunomodulatory role of arthropod saliva has been well documented, but evidence for an effect on Plasmodium sp. infectiousness remains controversial. Mosquito saliva may orient the immune response toward a Th2 profile, thereby priming a Th2 response against subsequent antigens, including Plasmodium. Orientation to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06414-11
更新日期:2012-06-01 00:00:00
abstract::Only 20 to 40% of human blood monocytes were capable of responding to chemotaxins in vitro. This limit is not due to restrictions of the in vitro system, but is due to the existence of a migrating subpopulation. Over a wide range, the number of cells migrating toward a given concentration of chemotaxin was directly pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00