Abstract:
:Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal secretory and inflammatory responses to STEC in the bovine intestine, serotypes that are associated with human disease (O103:H2 and O157:H7) were introduced into ligated mid-ileal loops in gnotobiotic and conventional calves, and fluid accumulation and recruitment of radiolabeled neutrophils were measured after 12 h. STEC serotype O103:H2, but not serotype O157:H7, elicited strong enteropathogenic responses. To determine if the inflammatory response to STEC O103:H2 in calves requires Shiga toxin 1 or intimate bacterial attachment to the intestinal epithelium, defined mutations were made in the stx1, eae, and tir genes. Our data indicate that some STEC induce intestinal inflammatory responses in calves by a mechanism that is independent of A/E-lesion formation, intimin, or Shiga toxin 1. This may have implications for strategies to reduce STEC carriage in cattle.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Stevens MP,Marchès O,Campbell J,Huter V,Frankel G,Phillips AD,Oswald E,Wallis TSdoi
10.1128/iai.70.2.945-952.2002keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
945-52issue
2eissn
0019-9567issn
1098-5522journal_volume
70pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2403-2408.1995
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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更新日期:1994-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.3.593-597.1990
更新日期:1990-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1976-06-01 00:00:00