Solubilization and partial characterization of the intestinal receptor for Escherichia coli heat-stable enterotoxin.

abstract：:An enzyme from Treponema denticola that hydrolyzes a synthetic trypsin substrate, N-alpha-benzoyl-L-arginine-p-nitroanilide (BAPNA), was purified to near homogeneity, as judged by gel electrophoresis. The molecular weight of the enzyme was estimated to be ca. 69,000 by sodium dodecyl sulfate-polyacrylamide gel electro...

journal_title：Infection and immunity

authors： Ohta K,Makinen KK,Loesche WJ

更新日期：1986-07-01 00:00:00

abstract：:Intracellular bacterial infections affect all vertebrates. Cultured fish are particularly vulnerable because no effective protection measures have been established since such infections emerged approximately 50 years ago. As in other vertebrates, the induction of cell-mediated immunity (CMI) plays an important role in...

journal_title：Infection and immunity

authors： Matsumoto M,Kubota T,Fujita S,Shiozaki K,Kishida S,Yamamoto A

更新日期：2019-10-18 00:00:00

abstract：:We have constructed an improved recombination-based in vivo expression technology (RIVET) and used it as a screening method to identify Vibrio cholerae genes that are transcriptionally induced during infection of infant mice. The improvements include the introduction of modified substrate cassettes for resolvase that ...

journal_title：Infection and immunity

authors： Osorio CG,Crawford JA,Michalski J,Martinez-Wilson H,Kaper JB,Camilli A

更新日期：2005-02-01 00:00:00

abstract：:Although the suckling mouse assay is widely used for the detection of heat-stable Escherichia coli enterotoxin (ST), few data have been published concerning the reproducibility, optimal growth, and test conditions of this assay. Four strains of toxigenic E. coli known to elaborate both heat-labile enterotoxin and ST o...

journal_title：Infection and immunity

authors： Giannella RA

更新日期：1976-07-01 00:00:00

abstract：:We previously showed the requirement of both T cells and gamma interferon (IFN-gamma)-producing non-T cells for the genetic resistance of BALB/c mice to the development of toxoplasmic encephalitis (TE). In order to define the role of IFN-gamma production and the perforin-mediated cytotoxicity of T cells in this resist...

journal_title：Infection and immunity

authors： Wang X,Kang H,Kikuchi T,Suzuki Y

更新日期：2004-08-01 00:00:00

abstract：:We explored the role of urokinase and tissue-type plasminogen activators (uPA and tPA), as well as the uPA receptor (uPAR; CD87) in mouse severe malaria (SM), using genetically deficient (-/-) mice. The mortality resulting from Plasmodium berghei ANKA infection was delayed in uPA(-/-) and uPAR(-/-) mice but was simila...

journal_title：Infection and immunity

authors： Piguet PF,Da Laperrousaz C,Vesin C,Tacchini-Cottier F,Senaldi G,Grau GE

更新日期：2000-07-01 00:00:00

abstract：:Using microarray technology, we studied the early differential expression of 3,528 genes in human meningothelial cells in response to meningococcal challenge. Thirty-two genes were up-regulated, and four were down-regulated. Those up-regulated included the tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-8 (b...

journal_title：Infection and immunity

authors： Wells DB,Tighe PJ,Wooldridge KG,Robinson K,Ala' Aldeen DA

更新日期：2001-04-01 00:00:00

abstract：:Penetration of the intestinal mucosa at areas of Peyer's patches is an important first step for Salmonella typhimurium to produce lethal systemic disease in mice. However, mutations in genes that are important for intestinal invasion result in only moderately decreased virulence of S. typhimurium for mice. Here we rep...

journal_title：Infection and immunity

authors： Bäumler AJ,Tsolis RM,Valentine PJ,Ficht TA,Heffron F

更新日期：1997-06-01 00:00:00

abstract：:Hot phenol-water extraction of smooth Brucella abortus and B. melitensis cells yielded a toxic fraction which was recovered from the phenol phase (fraction 5). Chemically, fractions 5 from both Brucella species were lipid-carbohydrate-protein-2 keto-3-deoxyoctulosonic acid complexes which were stable to heat and resis...

journal_title：Infection and immunity

authors： Leong D,Diaz R,Milner K,Rudbach J,Wilson JB

更新日期：1970-02-01 00:00:00

abstract：:Help from CD4 T cells is often important for the establishment of primary and memory CD8 T-cell responses. However, it has yet to be determined whether T helper polarization affects the delivery of help and/or whether responding CD8 T cells helped by Th1 or Th2 cells express distinct effector properties. To address th...

journal_title：Infection and immunity

authors： Ekkens MJ,Shedlock DJ,Jung E,Troy A,Pearce EL,Shen H,Pearce EJ

更新日期：2007-05-01 00:00:00

abstract：:Ferrets which had been orally dosed with 5 mg of Staphylococcal enterotoxin B (SEB) responded with an increase in subcutaneous temperature. At 75 min, the subcutaneous temperature was significantly higher (+ 0.9 degrees C +/- 0.38 degrees C, P < 0.007) than in control animals. Animals dosed with 1 or 2 mg of SEB respo...

journal_title：Infection and immunity

authors： Wright A,Andrews PL,Titball RW

更新日期：2000-04-01 00:00:00

abstract：:Previous studies have noted that the prevalence of certain gonococcal colony types is influenced by the physiological state of the human host. Consequently eight different gonococcal strains were passed on clear typing medium, and opaque (Op) and transparent (Tr) variants of heavily pilated (P++) bacteria were selecte...

journal_title：Infection and immunity

authors： Salit IE,Gotschlich EC

更新日期：1978-11-01 00:00:00

abstract：:Cell surface protein SSP-5 in the oral bacterium Streptococcus gordonii M5 binds human salivary agglutinin in a Ca(2+)-dependent reaction (D.R. Demuth, E.E. Golub, and D. Malamud, J. Biol. Chem. 265:7120-7126, 1990). The region of the gene encoding an N-terminal segment of a related polypeptide (SspA) in S. gordonii D...

journal_title：Infection and immunity

authors： Jenkinson HF,Terry SD,McNab R,Tannock GW

更新日期：1993-08-01 00:00:00

abstract：:Borrelia burgdorferi, a tick-borne bacterial pathogen, causes a disseminated infection involving multiple organs known as Lyme disease. Surface proteins can directly participate in microbial virulence by facilitating pathogen dissemination via interaction with host factors. We show here that a fraction of the B. burgd...

journal_title：Infection and immunity

authors： Nogueira SV,Smith AA,Qin JH,Pal U

更新日期：2012-01-01 00:00:00

abstract：:The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is involved in the utilization of heme as an iron source. Transcription of hmuO is activated by heme or hemoglobin and repressed by iron and DtxR. Previous studies with Escherichia coli showed that heme-dependent transcriptional activation of an h...

journal_title：Infection and immunity

authors： Bibb LA,King ND,Kunkle CA,Schmitt MP

更新日期：2005-11-01 00:00:00

abstract：:Subpopulations of spleen cells from scrapie virus-infected mice were used to determine the average virus content of infected cells in vivo at a time when virus was rapidly increasing in titer in lymphoreticular tissues. Comparison of the mean lethal doses of lysed to intact cells indicated averages of 2 to 6 infectiou...

journal_title：Infection and immunity

authors： Lavelle GC

更新日期：1973-06-01 00:00:00

abstract：:Three parameters of rotavirus infection, i.e., clinical disease, viral antigen in infected intestines, and infectious virus in feces, were assessed in infant mice nursed by mothers with or without preexisting rotavirus antibody. Diarrhea was the only consistent sign of clinical disease, and its course followed that of...

journal_title：Infection and immunity

authors： Little LM,Shadduck JA

更新日期：1982-11-01 00:00:00

abstract：:Larvae of Toxocara canis, a nematode parasite of dogs, infect humans, causing visceral and ocular larva migrans. In noncanid hosts, larvae neither grow nor differentiate but endure in a state of arrested development. Reasoning that parasite protein production is orientated to immune evasion, we undertook a random sequ...

journal_title：Infection and immunity

authors： Tetteh KK,Loukas A,Tripp C,Maizels RM

更新日期：1999-09-01 00:00:00

abstract：:Glucose has previously been shown to increase the in vitro phagocytosis of unopsonized Pseudomonas aeruginosa by freshly explanted murine peritoneal macrophages (PM) and cultivated alveolar macrophages (AM). This study examined the effect of glucose on the same phagocytosis process in human AM in order to determine wh...

journal_title：Infection and immunity

authors： Wong SY,Guerdoud LM,Cantin A,Speert DP

更新日期：1999-01-01 00:00:00

abstract：:Binding of radiolabeled human fibrinogen and aggregated beta-microglobulin was measured in 60 strains of beta-hemolytic group B streptococci. Positive fibrinogen binding was detected in seven of the strains. Six of the group B strains showed an uptake of aggregated beta 2-microglobulin. Four individual strains carried...

journal_title：Infection and immunity

authors： Schönbeck C,Björck L,Kronvall G

更新日期：1981-03-01 00:00:00

abstract：:The adherence of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin-4-sulfate (C4S). Although IRBC binding to C4S has been unequivocally established, the adherence characteristics of IRBCs at different stages of parasite development and through successive parasite g...

journal_title：Infection and immunity

authors： Madhunapantula SV,Achur RN,Gowda DC

更新日期：2007-09-01 00:00:00

abstract：:Infections caused by organisms of the Mycobacterium avium complex occur in approximately 50 to 60% of patients with AIDS. M. avium is an intracellular pathogen that survives and multiplies within mononuclear phagocytes. In this study, we investigated the uptake of M. avium grown within macrophages (intracellular growt...

journal_title：Infection and immunity

authors： Bermudez LE,Parker A,Goodman JR

更新日期：1997-05-01 00:00:00

abstract：:Chlamydia trachomatis is an obligately intracellular bacterial parasite of eucaryotic cells that undergoes a biphasic life cycle within a parasitophorous vacuole (PV) called an inclusion. The parasitophorous vacuolar membrane (PVM) constitutes a barrier between the replicating bacteria and the nutrient-rich environmen...

journal_title：Infection and immunity

authors： Heinzen RA,Hackstadt T

更新日期：1997-03-01 00:00:00

abstract：:The pneumocandins are potent antifungal agents of the echinocandin class which are under development for use as broad-spectrum antimycotic therapy. One important consideration for any new therapeutic class for treating serious fungal infections is the potential for drug resistance development. In this study we have is...

journal_title：Infection and immunity

authors： Kurtz MB,Abruzzo G,Flattery A,Bartizal K,Marrinan JA,Li W,Milligan J,Nollstadt K,Douglas CM

更新日期：1996-08-01 00:00:00

abstract：:Toxic protein(s) neutralized with anti-C2 toxic serum was extracted from Clostridium botulinum type C spores treated with an alkaline mercaptoethanol solution. C2 toxicity in the spores was located in the spore coat fraction and was more heat stable than that found in culture fluid. ...

journal_title：Infection and immunity

authors： Yamakawa K,Nishida S,Nakamura S

更新日期：1983-08-01 00:00:00

abstract：:We used an in vitro opsonophagocytic killing assay to measure the functional activity of antibody directed at the mucoid exopolysaccharide (MEP) antigen expressed by Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Rabbit antibodies raised to purified MEP were able to mediate phagocytic killing i...

journal_title：Infection and immunity

authors： Ames P,DesJardins D,Pier GB

更新日期：1985-08-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen with a low infectious dose that colonizes the colon in humans and can cause severe clinical manifestations such as hemolytic-uremic syndrome. The urease enzyme, encoded in the STEC chromosome, has been demonstrated to act as a virulence factor in o...

journal_title：Infection and immunity

authors： Steyert SR,Kaper JB

更新日期：2012-08-01 00:00:00

abstract：:Pools of rat antiserum to Plasmodium berghei had different levels of protective activity as assessed by a passive transfer test. Preincubation of parasite inocula with an effective pool before injection did not significantly enhance protective activity. Removal of the antiserum from preincubated parasite inocula aboli...

journal_title：Infection and immunity

authors： Brown IN,Phillips RS

更新日期：1974-12-01 00:00:00

abstract：:Protection against cholera has been correlated with the level of serum vibriocidal antibodies. The specificity of these vibriocidal antibodies was mostly to the lipopolysaccharide (LPS). We synthesized conjugates of detoxified LPS with cholera toxin (CT) and other proteins in order to elicit serum LPS antibodies with ...

journal_title：Infection and immunity

authors： Gupta RK,Szu SC,Finkelstein RA,Robbins JB

更新日期：1992-08-01 00:00:00

abstract：:Mice immunized intravenously 10 days earlier (but not those immunized 2 months earlier) with an attenuated Salmonella typhimurium SL3261 aroA live vaccine and tested for delayed-type hypersensitivity by injection of crude Salmonella extracts in the footpad can die within 24 to 48 h of an unexplained allergic reaction....

journal_title：Infection and immunity

authors： Mastroeni P,Villarreal-Ramos B,Harrison JA,Demarco de Hormaeche R,Hormaeche CE

更新日期：1994-06-01 00:00:00