Abstract:
:We have constructed an improved recombination-based in vivo expression technology (RIVET) and used it as a screening method to identify Vibrio cholerae genes that are transcriptionally induced during infection of infant mice. The improvements include the introduction of modified substrate cassettes for resolvase that can be positively and negatively selected for, allowing selection of resolved strains from intestinal homogenates, and three different tnpR alleles that cover a range of translation initiation efficiencies, allowing identification of infection-induced genes that have low-to-moderate basal levels of transcription during growth in vitro. A transcriptional fusion library of 8,734 isolates of a V. cholerae El Tor strain that remain unresolved when the vibrios are grown in vitro was passed through infant mice, and 40 infection-induced genes were identified. Nine of these genes were inactivated by in-frame deletions, and their roles in growth in vitro and fitness during infection were measured by competition assays. Four mutant strains were attenuated >10-fold in vivo compared with the parental strain, demonstrating that infection-induced genes are enriched in genes essential for virulence.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Osorio CG,Crawford JA,Michalski J,Martinez-Wilson H,Kaper JB,Camilli Adoi
10.1128/IAI.73.2.972-980.2005keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
972-80issue
2eissn
0019-9567issn
1098-5522pii
73/2/972journal_volume
73pub_type
杂志文章abstract::The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelia...
journal_title:Infection and immunity
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doi:10.1128/iai.68.12.6744-6749.2000
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journal_title:Infection and immunity
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doi:10.1128/IAI.60.2.406-415.1992
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.320-331.1990
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journal_title:Infection and immunity
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doi:10.1128/IAI.64.4.1140-1145.1996
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journal_title:Infection and immunity
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doi:10.1128/IAI.57.5.1517-1523.1989
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journal_title:Infection and immunity
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doi:10.1128/IAI.00424-17
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.423-428.1977
更新日期:1977-02-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1984-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.61.3.793-799.1993
更新日期:1993-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2660-2665.1989
更新日期:1989-09-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.59.6.1991-1996.1991
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更新日期:2001-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.136-141.1985
更新日期:1985-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.2.676-684.1997
更新日期:1997-02-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.122-131.1983
更新日期:1983-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.469-477.1981
更新日期:1981-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3620-3629.2000
更新日期:2000-06-01 00:00:00
abstract::To assess the relationship between the within-host diversity of malaria infections and the susceptibility of the host to subsequent infection, we genotyped 60 children's successive infections from birth through 3 years of life. MSP-1 Block2 genotypes were used to estimate the complexity of infection (COI). Malaria tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.12.7783-7792.2001
更新日期:2001-12-01 00:00:00
abstract::Of 202 captive hares studied, many of which had lesions on their external genital organs or testicular atrophy or both, 27% had positive serological tests for syphilis although dark-field examination of extracts of atrophic testes was negative. A total of 12 hares that were nonreactive for the serological test for syp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.1.231-234.1980
更新日期:1980-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.3876-3882.2004
更新日期:2004-07-01 00:00:00
abstract::We have determined the amounts of specific anti-class 1 outer membrane protein antibodies in sera from 25 patients during the course of systemic meningococcal disease, using purified class 1 protein as the sensitizing antigen in an enzyme-linked immunosorbent assay. The class 1 protein was obtained from a variant of s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1437-1443.1994
更新日期:1994-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7213-7223.2001
更新日期:2001-12-01 00:00:00
abstract::The Lyme disease spirochete, Borrelia burgdorferi, exists in a zoonotic cycle involving an arthropod tick and mammalian host. Dissemination of the organism within and between these hosts depends upon the spirochete's ability to traverse through complex tissues. Additionally, the spirochete outruns the host immune cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01228-12
更新日期:2013-06-01 00:00:00