Abstract:
:The epitope specificities of 13 hybridomas secreting monoclonal antibodies (MAbs) specific for pertussis toxin (PT) is described. Hybridoma lines were derived by the fusion of spleen cells from mice immunized with native PT, Formalin-detoxified PT, or isolated PT subunits (S1 to S5) with the myeloma line X63-Ag8.653. Five MAbs showed a toxin-neutralizing ability, which was demonstrated by use of a Chinese hamster ovary cell assay system and by a NAD glycohydrolase assay. All five toxin-neutralizing MAbs demonstrated high specificities for and reactivities with native PT but were unable to bind to denatured PT. One MAb was able to neutralize the enzymatic activity of PT. The other four neutralizing MAbs inhibited the binding of PT or PT subunits to the surface of Chinese hamster ovary cells, as shown by an immunofluorescence assay. All neutralizing MAbs reacted with purified S2-S4 or S3-S4 dimers but not with S4 alone. Three MAbs which recognized a common epitope shared by S2 and S3 (which are about 70% homologous at the DNA level) and one MAb which recognized S4 were not neutralizing. Isolated S2-S4 and S3-S4 dimers bound to Chinese hamster ovary cells. These results indicate that the majority of critical epitopes which elicit neutralizing antibody are conformation dependent.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lang AB,Ganss MT,Cryz SJ Jrdoi
10.1128/IAI.57.9.2660-2665.1989subject
Has Abstractpub_date
1989-09-01 00:00:00pages
2660-5issue
9eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::Chlamydia trachomatis is an obligate intracellular pathogen that causes sexually transmitted disease. In women, chlamydial infections may cause pelvic inflammatory disease (PID), ectopic pregnancy, and infertility. The role of antibodies in protection against a primary Chlamydia infection is unclear and was a focus of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00413-20
更新日期:2020-12-15 00:00:00
abstract::Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. Protein profiles of the C. pneumoniae isolates appeared to be the same and were distinct from the other Chlamydia spec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.93-97.1990
更新日期:1990-01-01 00:00:00
abstract::Members of our group reported recently that neisseria infection of human epithelial cells results in accelerated degradation of the major lysosomal integral membrane protein LAMP1 and that this is due to hydrolysis of this glycoprotein at its immunoglobulin A1 (IgA1)-like hinge by the neisseria type 2 IgA1 protease (L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.10.5001-5007.1998
更新日期:1998-10-01 00:00:00
abstract::The interaction of plasma fibronectin with C1q of the complement system has been demonstrated in the past several years. In addition, the antibody-independent binding of C1q to bacteria, as well as the binding of plasma fibronectin to bacteria, is well documented. This study examines whether the binding of C1q to bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.664-669.1985
更新日期:1985-09-01 00:00:00
abstract::We have reported that P388D1 cell line murine macrophages stimulated with lipopolysaccharide (LPS) from Actinobacillus actinomycetemcomitans release interleukin-1 (IL-1) inhibitor. The IL-1 inhibitor was purified from conditioned media of P388D1 cells stimulated with A. actinomycetemcomitans LPS for 72 h to homogeneit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.390-397.1994
更新日期:1994-02-01 00:00:00
abstract::Pasteurella multocida is the causative agent of fowl cholera in birds. In a previous study using signature-tagged mutagenesis, we identified a mutant, AL251, which was attenuated for virulence in mice and in the natural chicken host. Sequence analysis indicated that AL251 had an insertional inactivation of the gene wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3436-3443.2004
更新日期:2004-06-01 00:00:00
abstract::Ocular toxoplasmosis is a potentially blinding intraocular inflammation. The intent of this study was to investigate the role of Fas-FasL interaction in a murine model of acquired ocular toxoplasmosis induced by intracameral inoculation of Toxoplasma gondii. Intraocular inflammation, Fas and FasL expression on lymphoc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.928-935.1999
更新日期:1999-02-01 00:00:00
abstract::Brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. Smooth organisms present O polysaccharides (OPS) on their surface. These OPS help the bacteria avoid the bactericidal action of serum. The wboA gene, coding for the enzyme glycosyltransferase, is e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4407-4416.2001
更新日期:2001-07-01 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in controlling several aspects of immune responses, including the activation and differentiation of specific T cell subsets and antigen-presenting cells, thought to be relevant in the context of experimental Trypanosoma cruzi infec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00575-16
更新日期:2016-09-19 00:00:00
abstract::Immunologically competent mice and mice with defined immunological deficiencies were infected with Plasmodium yoelii. Splenomegaly, enhanced phagocytosis, and anemia were most marked in infected mice having intact thymic tissue. Whereas the spleens of infected nude mice increased minimally in size, the relative blood ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.728-731.1978
更新日期:1978-06-01 00:00:00
abstract::Immunoprecipitation of radiolabeled antigens with bovine antisera indicated that many Babesia bigemina and Babesia bovis merozoite proteins contain isolate-common epitopes, while at least 16 B. bigemina and 8 B. bovis proteins contain species-cross-reactive epitopes. One immunogenic, isolate-common, and species-specif...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1658-1660.1988
更新日期:1988-06-01 00:00:00
abstract::It was found that cells of Nocardia asteroides GUH-2 (virulent) were approximately 10 times more virulent than cells of N. asteroides 14759 (intermediate) and greater that 500 times more virulent than N. asteroides 10905 (avirulent) cells when early-stationary-phase cultures suspended in saline were injected intraven...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.995-1004.1977
更新日期:1977-06-01 00:00:00
abstract::Hot phenol-water extraction of smooth Brucella abortus and B. melitensis cells yielded a toxic fraction which was recovered from the phenol phase (fraction 5). Chemically, fractions 5 from both Brucella species were lipid-carbohydrate-protein-2 keto-3-deoxyoctulosonic acid complexes which were stable to heat and resis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-02-01 00:00:00
abstract::Monoclonal antibodies (MAbs) specific for Plasmodium falciparum rhoptry-associated protein 1 (RAP-1) were generated and tested for inhibition of parasite growth in vitro. The majority of indirect immunofluorescence assay (IFA)-positive MAbs raised against recombinant RAP-1 positions 23 to 711 (rRAP-1(23-711)) recogniz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2558-2568.2001
更新日期:2001-04-01 00:00:00
abstract::Legionnaires' disease is clinically manifested as severe pneumonia caused by Legionella pneumophila. However, the dendritic cell (DC)-centered immunological framework of the host defense against L. pneumophila has not been fully delineated. For this study, we focused on a potent chemoattractant for lymphocytes, fracta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5350-5357.2005
更新日期:2005-09-01 00:00:00
abstract::In vitro, Clostridium perfringens enterotoxin (CPE) binds to human ileal epithelium and induces morphological damage concurrently with reduced short-circuit current, transepithelial resistance, and net water absorption. CPE also binds to the human colon in vitro but causes only slight morphological and transport chang...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8407-8410.2005
更新日期:2005-12-01 00:00:00
abstract::Genetic diversity and relationships among 109 isolates of Neisseria meningitidis obtained from throat cultures of healthy individuals in Norway in 1984 were assessed by analyzing electrophoretically demonstrable allelic variation at 15 enzyme-encoding chromosomal genes. Seventy-eight distinctive electrophoretic types ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2060-2068.1988
更新日期:1988-08-01 00:00:00
abstract::Since 1984, it has been known that spotted fever group rickettsiosis exists in Japan. We isolated three strains of the causative rickettsiae, designated Katayama, Misaka, and Abe, from patients with the disease and studied the characteristics of the isolates. Nude mice and cyclophosphamide-treated mice died after infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.887-892.1990
更新日期:1990-04-01 00:00:00
abstract::Human opsonins directed against specific meningococcal outer membrane structures in sera obtained during meningococcal disease were quantified with a recently developed antigen-specific, opsonin-dependent phagocytosis and oxidative burst assay. Outer membrane vesicles (OMVs) and PorA (class 1) and PorB (class 3) prote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2552-2560.1999
更新日期:1999-05-01 00:00:00
abstract::The acute phase of Chagas' disease is accompanied by immunosuppression. To explore the underlying mechanism(s), we used an in vitro culture system in which the capacities of activated human peripheral blood mononuclear cells to express interleukin-2 receptors (IL-2R) and proliferate are markedly inhibited in the prese...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.8.2301-2305.1989
更新日期:1989-08-01 00:00:00
abstract::Alpha-2,3-sialyltransferase (Lst) is expressed on the outer membrane of Neisseria gonorrhoeae and Neisseria meningitidis and sialylates surface lipooligosaccharide (LOS), facilitating resistance to complement-mediated killing. The enzyme is constitutively expressed from a single gene (lst) and does not undergo antigen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2637-2650.2006
更新日期:2006-05-01 00:00:00
abstract::Escherichia coli strains produce at least two heat-stable enterotoxins, STa and STb. STa is well known to be important in the pathogenesis of human diarrheal disease; the role of STb has not been defined. Fifty-two E. coli strains recovered from human diarrheal illness in northeast Brazil or Bangladesh were examined i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.323-325.1986
更新日期:1986-04-01 00:00:00
abstract::When tested for their activity in suppressing the acquisition of immunity during acute Besnoitia infection of hamsters, antilymphocyte serum (ALS), aminopterin, cyclophosphamide, cortisol, and whole-body irradiation were the most active agents and effectively blocked the development of immunity during 4 to 12 days of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.6.756-761.1971
更新日期:1971-06-01 00:00:00
abstract::Monoclonal antibodies were produced against the HAR-13 strain (A) of Chlamydia trachomatis. By an indirect immunofluorescence technique, three clones (1-7, 2-8, 9F) were found to produce antibody that reacted only with serotype A strains. Clone 3-5 produced antibody that cross-reacted with serotypes A, C, H, I, and J....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.1273-1278.1982
更新日期:1982-12-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa is a major cause of sepsis in severely burned patients. If it is not eradicated from the wound, it translocates to the bloodstream, causing sepsis, multiorgan failure, and death. We recently described the P. aeruginosa heparinase-encoding gene, hepP, whose expression w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00755-17
更新日期:2017-12-19 00:00:00
abstract::The innate immune response to Pneumocystis infection is not well understood. In this study, normal C57BL/6 mouse alveolar macrophages were found to respond to Pneumocystis murina organisms through Toll-like receptor 2 (TLR2), leading to the nuclear translocation of NF-kappaB and the production of proinflammatory cytok...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1857-1864.2006
更新日期:2006-03-01 00:00:00
abstract::Development of an effective vaccine against Leishmania infection is a priority of tropical disease research. We have recently demonstrated protection against Leishmania major in the murine and nonhuman primate models with individual or combinations of purified leishmanial recombinant antigens delivered as plasmid DNA ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4215-4225.2002
更新日期:2002-08-01 00:00:00
abstract::Serological tests used in current brucellosis eradication schemes, such as bacterial tube agglutination, do not readily distinguish between infected animals and those immunized with strain 19 or 45/20 Brucella abortus vaccines. In this study, sera from naturally infected cattle were used to identify serologically impo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Chronic lung infections with Pseudomonas aeruginosa biofilms are associated with refractory and fatal pneumonia in cystic fibrosis (CF). In this study, a group of genomically diverse P. aeruginosa isolates were compared with the reference strain PAO1 to assess the roles of motility, twitching, growth rate, and overpro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.133-144.2004
更新日期:2004-01-01 00:00:00
abstract::Salmonellae possess the ability to adhere to and invade macrophages and in so doing trigger a number of intracellular events that are associated with cellular activation. As an initial approach to defining the mechanisms by which invasive salmonellae alter macrophage function, we have explored the impact of Salmonella...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1820-1826.1995
更新日期:1995-05-01 00:00:00