Abstract:
:The innate immune response to Pneumocystis infection is not well understood. In this study, normal C57BL/6 mouse alveolar macrophages were found to respond to Pneumocystis murina organisms through Toll-like receptor 2 (TLR2), leading to the nuclear translocation of NF-kappaB and the production of proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha) and chemokine macrophage inflammatory protein 2 (MIP-2). P. murina stimulation of normal alveolar macrophages from C57BL/6 mice resulted in increased TLR2 transcription but not increased TLR4 transcription. In gain-of-function studies with HEK293 cells expressing TLR2 or TLR4, only TLR2 was found to stimulate an NF-kappaB response to P. murina. TNF-alpha and MIP-2 production in response to P. murina by mouse alveolar macrophages was inhibited by a monoclonal antibody that specifically blocked the ligand-binding ability of TLR2. Alveolar macrophages from TLR2 knockout (TLR2-/-) mice showed little increase in TNF-alpha and MIP-2 mRNA levels upon P. murina stimulation. An in vivo study showed that TLR2-/- mice challenged with P. murina had reduced cytokine responses. These results indicate that TLR2 plays a major role in the innate immune response to P. murina.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Zhang C,Wang SH,Lasbury ME,Tschang D,Liao CP,Durant PJ,Lee CHdoi
10.1128/IAI.74.3.1857-1864.2006keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1857-64issue
3eissn
0019-9567issn
1098-5522pii
74/3/1857journal_volume
74pub_type
杂志文章abstract::Immunocompromised patients are more susceptible to recurrent nontyphoidal Salmonella (NTS) bacteremia. A key manifestation of HIV infection is the loss of CD4 T cells, which are crucial for immunity to Salmonella infection. We characterized the consequences of CD4 T cell depletion in mice where virulent Salmonella est...
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.60.11.4542-4548.1992
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journal_title:Infection and immunity
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doi:10.1128/IAI.56.1.128-134.1988
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.9.2336-2342.1988
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.2.580-586.1991
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journal_title:Infection and immunity
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doi:10.1128/IAI.60.9.3916-3917.1992
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.62.7.2800-2805.1994
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.355-362.1976
更新日期:1976-08-01 00:00:00
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doi:10.1128/IAI.00605-10
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journal_title:Infection and immunity
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doi:10.1128/IAI.64.5.1559-1564.1996
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journal_title:Infection and immunity
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doi:10.1128/IAI.00439-09
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1813-1819.1992
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.913-918.1996
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.740-745.1978
更新日期:1978-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.323-325.1986
更新日期:1986-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.444-452.1977
更新日期:1977-02-01 00:00:00
abstract::Yersinia pestis KIM5 was found to be cytotoxic for the IC21 and P388D1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from C57BL/6 mice. Affected cells phagocytosed KIM5 inefficiently, became spherical, detached readily from culture dishes, and retained 51Cr poorly. The cytotoxic effect wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.788-794.1986
更新日期:1986-03-01 00:00:00
abstract::Bordetella bronchiseptica is a Gram-negative bacterium that infects and causes disease in a wide variety of animals. B. bronchiseptica also infects humans, thereby demonstrating zoonotic transmission. An extensive characterization of human B. bronchiseptica isolates is needed to better understand the distinct genetic ...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
abstract::A superoxide dismutase (SOD) gene from the obligate intracellular bacterium Coxiella burnetii has been cloned, and its DNA sequence has been determined and expressed in Escherichia coli. The gene was identified on pSJR50, a pHC79-derived genomic clone, by using the polymerase chain reaction with degenerate oligonucleo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3814-3823.1992
更新日期:1992-09-01 00:00:00