Abstract:
:We have reported that P388D1 cell line murine macrophages stimulated with lipopolysaccharide (LPS) from Actinobacillus actinomycetemcomitans release interleukin-1 (IL-1) inhibitor. The IL-1 inhibitor was purified from conditioned media of P388D1 cells stimulated with A. actinomycetemcomitans LPS for 72 h to homogeneity by a four-step procedure: acetic acid extraction from conditioned media; Bio-Gel P-60 gel filtration chromatography; DEAE-Sepharose CL-6B column chromatography; and reverse-phase high-performance liquid chromatography on a C18 hydrophobic support. The purified IL-1 inhibitor gave a single band of protein with a molecular mass of 26 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified IL-1 inhibitor was a heat- and acid-stable protein that was inactivated by digestion with trypsin and reduction with dithiothreitol. This inhibitory factor suppressed the proliferation of C3H/HeJ mouse thymocytes and the proliferation of IL-1-dependent cell lines, D10.G4.1 and RPMI 1788, induced by IL-1. However, this inhibitor did not affect the proliferation of IL-2-dependent CTLL-2 cells induced by IL-2, the proliferation of C3H/HeJ mouse thymocytes stimulated with a mitogenic dose of concanavalin A, and the proliferation of IL-6-dependent B9 cells induced by IL-6. Furthermore, the IL-1 inhibitor significantly blocked stimulation of bone resorption in organ cultures of newborn mouse calvaria and inhibited the osteoclast-like cell formation in mouse marrow cultures. A monoclonal antibody prepared against the purified IL-1 inhibitor reacted with mouse recombinant IL-1 receptor antagonist (rIL-1ra), and a polyclonal antibody to mouse rIL-1ra reacted with the IL-1 inhibitor by Western blot (immunoblot) analysis. These results indicate that the IL-1 inhibitor is an identical molecule to rIL-1ra, suggesting that the IL-1 inhibitor (IL-1ra) released by macrophages stimulated with LPS from A. actinomycetemcomitans may play an important mediative role in the development of periodontal disease.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nishihara T,Ohsaki Y,Ueda N,Saito N,Mundy GRdoi
10.1128/IAI.62.2.390-397.1994subject
Has Abstractpub_date
1994-02-01 00:00:00pages
390-7issue
2eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Salmonella is a widespread zoonotic enteropathogen that causes gastroenteritis and fatal typhoidal disease in mammals. During systemic infection of mice, Salmonella enterica serovar Typhimurium resides and replicates in macrophages within the "Salmonella-containing vacuole" (SCV). It is surprising that the substrates ...
journal_title:Infection and immunity
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doi:10.1128/IAI.00093-09
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journal_title:Infection and immunity
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doi:10.1128/IAI.59.6.2097-2104.1991
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journal_title:Infection and immunity
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doi:10.1128/IAI.58.1.205-213.1990
更新日期:1990-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.419-429.1974
更新日期:1974-02-01 00:00:00
abstract::Candida albicans is a ubiquitous mucosal commensal that is normally prevented from causing acute or chronic invasive disease. Neutrophils contribute to protection in oral infection but exacerbate vulvovaginal candidiasis. To dissect the role of neutrophils during mucosal candidiasis, we took advantage of a new, transp...
journal_title:Infection and immunity
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abstract::The production of interleukin-6 (IL-6) and its possible relationship to host resistance and inflammatory response to Pneumocystis carinii infection were examined in mice with severe combined immunodeficiency (SCID mice). IL-6 activity was detected in the serum and lungs of P. carinii-infected mice but not in mice free...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.97-102.1993
更新日期:1993-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2545-2552.1993
更新日期:1993-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2327-2331.1987
更新日期:1987-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.4069-4075.1990
更新日期:1990-12-01 00:00:00
abstract::When phagocytosis of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) takes place on the surface of cultured human endothelial cells, the endothelial monolayers are damaged by lysosomal enzymes that are released by the PMN. Because PMN can phagocytose opsonized as well as unopsonized staphylococci on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1455-1460.1987
更新日期:1987-06-01 00:00:00
abstract::Most Escherichia coli isolates can express type 1 fimbriae with mannose-specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type 1 fimbriae. The abs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2794-2798.1996
更新日期:1996-07-01 00:00:00
abstract::In general there is a poor correlation between serum lipopolysaccharide (LPS; the biologically active constituent of endotoxin) levels and mortality in septic patients. The objective of this study was to determine if chemical, structural, or biological differences among LPS from different clinical isolates of gram-neg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.1899-1904.2000
更新日期:2000-04-01 00:00:00
abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2262-2272.2005
更新日期:2005-04-01 00:00:00
abstract::Legionella pneumophila is ingested by both human macrophages and amoebae, and it multiplies within similar endocytic compartments in both eukaryotic species. Inhibitors of eukaryotic protein synthesis, such as cycloheximide and emetine, had no effect on the uptake of L. pneumophila by macrophages but completely abolis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1860-1866.1994
更新日期:1994-05-01 00:00:00
abstract::Platelets aggregate in response to an adhesin and the platelet aggregation-associated protein (PAAP) expressed on the cell surfaces of certain strains of Streptococcus sanguis. We sought to identify the corresponding PAAP receptor and accessory adhesin binding sites on platelets. Since the adhesion(s) of S. sanguis fo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3628-3633.1995
更新日期:1995-09-01 00:00:00
abstract::When immunocompetent mice are inoculated with Borrelia burgdorferi, they develop acute arthritis and carditis that undergo spontaneous regression despite the persistence of infection. Specific T- and/or B-cell immunity appears to be necessary for resolution of disease manifestations. Humoral immune responses to B. bur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2104-2107.1993
更新日期:1993-05-01 00:00:00
abstract::The interaction of Borrelia burgdorferi, the causative agent of Lyme borreliosis, with phagocytic cells induces the activation of NF-kappaB and the expression of proinflammatory cytokines including tumor necrosis factor alpha (TNF-alpha). B. burgdorferi-induced TNF-alpha production is also dependent on the activation ...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
abstract::Cytomegalovirus (CMV) infection is common after renal transplantation, and cytomegaloviruria occurs in about two-thirds of the recipients. These observations suggest that the allografts may be a site of latent infection with CMV and its reactivation may be the source of viruria. To investigate this possibility, 130 ki...
journal_title:Infection and immunity
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doi:10.1128/IAI.19.2.699-703.1978
更新日期:1978-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
abstract::The blood-stage development of malaria parasites is initiated by the invasion of merozoites into susceptible erythrocytes. Specific receptor-ligand interactions must occur for the merozoites to first attach to and then invade erythrocytes. Because the invasion process is essential for the parasite's survival and the m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5581-5586.1994
更新日期:1994-12-01 00:00:00
abstract::Francisella tularensis is a facultative intracellular bacterial pathogen and the causative agent of tularemia. After infection of macrophages, the organism escapes from its phagosome and replicates to high density in the cytosol, but the bacterial factors required for these aspects of virulence are incompletely define...
journal_title:Infection and immunity
pub_type: 杂志文章
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abstract::Ehrlichia chaffeensis is an obligately intracellular gram-negative bacterium and is the etiologic agent of human monocytic ehrlichiosis (HME). Although E. chaffeensis induces the generation of several cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglycan. Bioinfomatic analys...
journal_title:Infection and immunity
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更新日期:2008-08-01 00:00:00
abstract::An improved enterocyte adhesion assay has been used to examine a collection of 44 strains of enterotoxigenic Escherichia coli (ETEC) for their ability to adhere to the brush border of isolated human duodenal enterocytes. Fourteen strains showed good adhesion; in each case the ability to adhere correlated with the prod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.824-831.1985
更新日期:1985-06-01 00:00:00
abstract::Tuberculosis continues to ravage humanity, killing 2 million people yearly. Most cases occur in areas of the world to which the disease is endemic, where almost everyone is vaccinated early in life with Mycobacterium bovis BCG, the currently available vaccine against tuberculosis. Thus, while more-potent vaccines are ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4676-4683.2005
更新日期:2005-08-01 00:00:00
abstract::A purified A-antigen preparation of Blastomyces dermatitidis was determined to be composed of five major glycoprotein bands, visible with Coomassie blue and periodic acid-Schiff staining of polyacrylamide gels. At least 20 additional protein bands were detected by using a silver stain, which was 100 times more sensiti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.171-177.1981
更新日期:1981-07-01 00:00:00
abstract::The major outer membrane protein (P2) of Haemophilus influenzae type b (Hib) with an apparent molecular weight of 37,000 to 40,000 has been previously shown to function as a porin and also as a target for antibodies protective against experimental Hib disease. The gene encoding the Hib P2 protein was cloned by using a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2709-2716.1988
更新日期:1988-10-01 00:00:00
abstract::Bactericidal/permeability-increasing protein (BPI), a human neutrophil granule protein, has been shown to bind lipopolysaccharide (LPS) and neutralize LPS-mediated cytokine production in adherent monocytes and the whole-blood system. In this study we investigated the ability of recombinant human BPI (rBPI) to inhibit ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3930-3936.1994
更新日期:1994-09-01 00:00:00
abstract::Among a number of laboratory strains of Listeria monocytogenes used in experimental infection, strain LO28 is highly capable of inducing robust beta interferon (IFN-β) production in infected macrophages. In this study, we investigated the molecular mechanism of the IFN-β-inducing ability of LO28 by comparing it with t...
journal_title:Infection and immunity
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doi:10.1128/IAI.06162-11
更新日期:2012-07-01 00:00:00