Abstract:
:Salmonella is a widespread zoonotic enteropathogen that causes gastroenteritis and fatal typhoidal disease in mammals. During systemic infection of mice, Salmonella enterica serovar Typhimurium resides and replicates in macrophages within the "Salmonella-containing vacuole" (SCV). It is surprising that the substrates and metabolic pathways necessary for growth of S. Typhimurium within the SCV of macrophages have not been identified yet. To determine whether S. Typhimurium utilized sugars within the SCV, we constructed a series of S. Typhimurium mutants that lacked genes involved in sugar transport and catabolism and tested them for replication in mice and macrophages. These mutants included a mutant with a mutation in the pfkAB-encoded phosphofructokinase, which catalyzes a key committing step in glycolysis. We discovered that a pfkAB mutant is severely attenuated for replication and survival within RAW 264.7 macrophages. We also show that disruption of the phosphoenolpyruvate:carbohydrate phosphotransferase system by deletion of the ptsHI and crr genes reduces S. Typhimurium replication within RAW 264.7 macrophages. We discovered that mutants unable to catabolize glucose due to deletion of ptsHI, crr, and glk or deletion of ptsG, manXYZ, and glk showed reduced replication within RAW 264.7 macrophages. This study proves that S. Typhimurium requires glycolysis for infection of mice and macrophages and that transport of glucose is required for replication within macrophages.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Bowden SD,Rowley G,Hinton JC,Thompson Adoi
10.1128/IAI.00093-09subject
Has Abstractpub_date
2009-07-01 00:00:00pages
3117-26issue
7eissn
0019-9567issn
1098-5522pii
IAI.00093-09journal_volume
77pub_type
杂志文章abstract::A total of 32 patients with Plasmodium falciparum malaria were studied. Of these, 23 had benign infections, and 9 had typical cerebral malaria. Cryoglobulins, circulating immune complexes detected by a C1q-binding assay, and hypocomplementemia were found in eight of nine patients with cerebral malaria. Raised levels o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.530-535.1981
更新日期:1981-02-01 00:00:00
abstract::Whole blood and plasma from animals in the acute stage of leptospirosis contained a toxic factor which produced a cytopathic effect on fibroblastic L cell monolayers. Firm adsorption of cytotoxic factor (CTF) to L cells occurred within 1 h. The highest titer of CTF in plasma was reached at 24 h and declined after 48 h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.3.401-405.1973
更新日期:1973-09-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis is a major cause of food-borne diseases associated with consumption of shell eggs. Clinical isolates of S. enterica serovar Enteritidis exhibit a wide spectrum of virulence in mice. A highly virulent isolate (SE2472) was previously shown to be more resistant in vitro than other ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.451-461.2002
更新日期:2002-02-01 00:00:00
abstract::The endothelium is a specific target for Bartonella henselae, and endothelial cell infection represents an important step in the pathogenesis of cat scratch disease and bacillary angiomatosis. Mechanisms of Bartonella-endothelial cell interaction as well as signaling pathways involved in target cell activation were an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5088-5097.2001
更新日期:2001-08-01 00:00:00
abstract::We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5alpha and ChBac5, to detailed analysis. OaBac5alpha and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4106-4111.1999
更新日期:1999-08-01 00:00:00
abstract::Invasive fungal infections represent an increasing problem associated with high mortality. The present study was undertaken to identify leukocyte subsets that are activated by hyphal fragments in a whole-human-blood model, as well as to examine the involvement of CD14 and Toll-like receptors (TLRs) in activation of mo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2402-2406.2001
更新日期:2001-04-01 00:00:00
abstract::DNA microarrays provide an opportunity to combine the principles of signature-tagged mutagenesis (STM) with microarray technology to identify potentially important bacterial virulence genes. The scope of DNA microarrays allows for less laborious screening on a much larger scale than possible by STM alone. We have adap...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5438-5449.2005
更新日期:2005-09-01 00:00:00
abstract::The microfilament inhibitors cytochalasins B and D have been traditionally used to indirectly evaluate the requirement for actin in the uptake of invasive bacterial pathogens by nonprofessional phagocytes. Through their effects on microfilaments, both cytochalasins also impart profound alterations in cellular morpholo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3187-3193.1990
更新日期:1990-10-01 00:00:00
abstract::Outer surface protein A (OspA) of the Lyme disease spirochete is primarily produced in the tick vector. OspA, which is a receptor for attaching spirochetes to the tick gut, is down regulated as the spirochetes leave the tick and enter the mammalian host. Although OspA is not a major antigen produced in the mammal, the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01307-06
更新日期:2007-06-01 00:00:00
abstract::The oral streptococcal group (mitis phylogenetic group) currently consists of nine recognized species, although the group has been traditionally difficult to classify, with frequent changes in nomenclature over the years. The pneumococcus (Streptococcus pneumoniae), an important human pathogen, is traditionally distin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1374-1382.2000
更新日期:2000-03-01 00:00:00
abstract::In the development of vaccines capable of providing immunity against brucellosis, Cu-Zn superoxide dismutase (SOD) has been demonstrated to be one of the protective immunogens of Brucella abortus. In an earlier study, we provided strong evidence that intramuscular injection with a plasmid DNA carrying the SOD gene (pc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2081-2087.2004
更新日期:2004-04-01 00:00:00
abstract::Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58...
journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
doi:10.1128/IAI.66.1.356-360.1998
更新日期:1998-01-01 00:00:00
abstract::Previous passive antibody transfer experiments have indicated that immunity to a 46-kilodalton membrane glycoprotein (M-2) of Leishmania amazonensis may protect against infection with this parasite. In the studies described in this paper, we investigated the ability of the purified M-2 molecule to elicit a protective ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3272-3279.1988
更新日期:1988-12-01 00:00:00
abstract::Treponema denticola is a cultivable oral spirochete which perturbs the cytoskeleton in cultured cells of oral origin, but intracellular signalling pathways by which it affects actin assembly are largely unknown. As the outer membrane (OM) of Treponema denticola disrupts actin-dependent processes that normally require ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.703-709.1998
更新日期:1998-02-01 00:00:00
abstract::A large-scale DNA vaccination trial was performed with sheep to investigate whether an antigen targeted by CTLA-4 enhanced and accelerated the humoral immune response. Vaccination with genetically detoxified phospholipase D (DeltaPLD) has been shown to be effective, at least partially, against Corynebacterium pseudotu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::The selection of influenza B virus recombinants from plaques in bovine kidney cell monolayers is described. Two sets of recombinants were each derived from parents of high and low virulence for humans, respectively. Recombination frequency was apparently high, and reassortment of genes made it possible to obtain atten...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.347-353.1977
更新日期:1977-02-01 00:00:00
abstract::Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of bacterial lipopolysaccharide (LPS) and lipoprotein, respectively, were compared for their mitogenic activities on splenocytes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1382-1384.1988
更新日期:1988-05-01 00:00:00
abstract::The gene encoding the 35-kDa immunogenic Treponema pallidium subsp. pallidum (T. pallidum) membrane protein C, TmpC, was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence carries on N-terminal signal sequence with a four-amino-acid motif, which is characteristic for bacterial lipopr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3536-3546.1991
更新日期:1991-10-01 00:00:00
abstract::The 34-kilodalton (kDa) antigen of Treponema pallidum subsp. pallidum (T. pallidum) is a pathogen-specific integral membrane protein. DNA sequence analysis of the cloned gene revealed an open reading frame encoding a primary product of 204 residues with a molecular mass of 22,087 daltons. Sequences that correspond to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3314-3323.1989
更新日期:1989-11-01 00:00:00
abstract::We identified nonhemolytic mutants of Listeria monocytogenes that were severely deficient in their ability to invade mammalian nonprofessional phagocytes. These mutants were generated spontaneously or by means of transposon Tn916 mutagenesis. In terms of their extracellular proteins, the noninvasive mutants were defic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.3988-3995.1990
更新日期:1990-12-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a common cause of otitis media and respiratory tract infections. Outer membrane proteins (OMP) and lipooligosaccharide (LOS) are major surface antigens of NTHi and potential vaccine candidates. De-O-acylated LOS (dLOS) or oligosaccharide (OS) was coupled to total OMP to for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5508-5513.1999
更新日期:1999-10-01 00:00:00
abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth, and these molecules induce an inflammatory response in the human host. The proinflammatory molecules include peptidoglycan monomers, peptidoglycan dimers, and free peptides. These molecules can be released by the actions of lytic transglycosylases o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00833-18
更新日期:2019-01-24 00:00:00
abstract::The surface antigen makeup of metacyclic trypomastigote forms of strain G of Trypanosoma cruzi, which produce a subpatent infection in mice, differed from those of the virulent strains Y and CL. A 100,000-molecular-weight protein, barely detectable on the Y or CL cell surface, appeared as the main surface antigen of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.836-839.1983
更新日期:1983-05-01 00:00:00
abstract::Infection with Histoplasma capsulatum results in a subclinical infection in immunocompetent hosts due to an effective cellular immune response. By contrast, immunodeficient individuals can have a severe disseminated and potentially fatal disease. In a previous study, it was demonstrated that normal mice infected intra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.936-942.1997
更新日期:1997-03-01 00:00:00
abstract::Helicobacter pylori infection of the stomach causes an active immune response that includes stimulation of inducible nitric oxide (NO) synthase (iNOS) expression. Although NO can kill H. pylori, the bacterium persists indefinitely, suggesting that NO production is inadequate. We determined if the NO derived from iNOS ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00578-07
更新日期:2007-09-01 00:00:00
abstract::An apparent inhibition of induction of mouse hepatic tryptophan oxygenase by endotoxin has been reported previously, as evidenced by low catalytic activity. This could be due either to decreased tryptophan oxygenase levels or to inactivation of existing enzyme molecules. To resolve this question, the enzyme was quanti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.534-539.1973
更新日期:1973-10-01 00:00:00
abstract::Tick saliva has potent immunomodulatory properties. In arthropod-borne diseases, this effect is largely used by microorganisms to increase their pathogenicity and to evade host immune responses. We show that in Lyme borreliosis, tick salivary gland extract and a tick saliva protein, Salp15, inhibit in vitro keratinocy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00482-10
更新日期:2011-02-01 00:00:00
abstract::The aroC gene of the facultative intracellular pathogen Brucella suis was cloned and sequenced. The cloned aroC gene complements Escherichia coli and Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.547-550.2001
更新日期:2001-01-01 00:00:00
abstract::The antiphagocytic effect of the Staphylococcus aureus capsule is known to be related to its ability to interfere with opsonization by normal human serum. In this study, evidence is presented with isolated cell surface components which indicates that the capsule hinders opsonization by masking cell wall peptidoglycan....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.502-508.1979
更新日期:1979-02-01 00:00:00
abstract::The microbicidal myeloperoxidase (MPO)-H2O2-chloride system strongly inhibits Escherichia coli DNA synthesis. Also, cell envelopes from MPO-treated E. coli cells lose their ability to interact with hemimethylated DNA sequences of oriC, the chromosomal origin of replication, raising the prospect that suppression of DNA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2655-2659.1998
更新日期:1998-06-01 00:00:00