Targeting improves the efficacy of a DNA vaccine against Corynebacterium pseudotuberculosis in sheep.

Abstract:

:A large-scale DNA vaccination trial was performed with sheep to investigate whether an antigen targeted by CTLA-4 enhanced and accelerated the humoral immune response. Vaccination with genetically detoxified phospholipase D (DeltaPLD) has been shown to be effective, at least partially, against Corynebacterium pseudotuberculosis, the causal agent of caseous lymphadenitis in sheep. CTLA-4 binds to B7 on antigen-presenting cells and thus was used to direct the fusion antigens to sites of immune induction. Here we demonstrated that targeting DeltaPLD as a CTLA-4 fusion protein significantly enhanced the speed, magnitude, and longevity of the antibody response compared to that obtained with DNA encoding DeltaPLD. While all groups of sheep vaccinated with DNA encoding DeltaPLD were afforded better protection against an experimental challenge with C. pseudotuberculosis than those immunized with an irrelevant plasmid or those left unimmunized, the best protection was provided by the targeted DNA vaccine. We propose that targeting antigens to antigen-presenting cells offers a generic strategy for enhancing the efficacy of DNA vaccines.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Chaplin PJ,De Rose R,Boyle JS,McWaters P,Kelly J,Tennent JM,Lew AM,Scheerlinck JP

keywords:

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

6434-8

issue

12

eissn

0019-9567

issn

1098-5522

journal_volume

67

pub_type

杂志文章
  • Lipopolysaccharide structures in Enterobacteriaceae, Pseudomonas aeruginosa, and Vibrio cholerae are immunologically related to Campylobacter spp.

    abstract::To determine whether lipopolysaccharide (LPS) structures of Campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the LPS of these strains with immune rabbit serum raised against six Campylobacter jejuni strains and two Campylobacter f...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.51.1.204-208.1986

    authors: Perez-Perez GI,Hopkins JA,Blaser MJ

    更新日期:1986-01-01 00:00:00

  • Experimental cholera in germfree suckling mice.

    abstract::Vibrio cholerae V86 El Tor Inaba caused experimental cholera in germfree ICR suckling mice. Sucklings born of gnotobiotic mothers monoassociated with the same strain were completely protected against live oral homologous challenge. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.34.1.296-298.1981

    authors: Shimamura T,Tazume S,Hashimoto K,Sasaki S

    更新日期:1981-10-01 00:00:00

  • Mixed periodontal Th1-Th2 cytokine profile in Actinobacillus actinomycetemcomitans-specific osteoprotegerin ligand (or RANK-L)- mediated alveolar bone destruction in vivo.

    abstract::The Th1/Th2 cytokines involved in human periodontitis remain unclear; therefore, we established a humanized mouse model to investigate this issue in Actinobacillus actinomycetemcomitans-mediated periodontal infection. Quantitative-PCR analysis clearly demonstrates a predominantly mixed Th1 and Th2 expression profile a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.9.5269-5273.2002

    authors: Teng YT

    更新日期:2002-09-01 00:00:00

  • Elimination of Leishmania donovani amastigotes by activated macrophages.

    abstract::Tissue macrophages are the obligatory host cells for Leishmania donovani, the causative agent of visceral leishmaniasis. In this study we sought to determine whether activated macrophages, as defined by the functional criterion of tumor cell cytotoxicity, were also able to exert a microbicidal effect on ingested L. do...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.33.3.918-926.1981

    authors: Haidaris CG,Bonventre PF

    更新日期:1981-09-01 00:00:00

  • Influence of cadmium on the phagocytic and microbicidal activity of murine peritoneal macrophages, pulmonary alveolar macrophages, and polymorphonuclear neutrophils.

    abstract::A significant depression in the phagocytic capacity of elicited peritoneal macrophages, pulmonary alveolar macrophages, and elicited peritoneal polymorphonucleated neutrophils was manifested when the cells were incubated in medium containing cadmium chloride. With the exception of the neutrophils, a similar influence ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.22.2.378-381.1978

    authors: Loose LD,Silkworth JB,Simpson DW

    更新日期:1978-11-01 00:00:00

  • Functional roles for C5a and C5aR but not C5L2 in the pathogenesis of human and experimental cerebral malaria.

    abstract::The host immune response plays an important role in the onset and progression of cerebral malaria (CM). The complement system is an essential component of the innate immune response to malaria, and its activation generates the anaphylatoxin C5a. To test the hypothesis that C5a signaling contributes to the pathogenesis...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01246-13

    authors: Kim H,Erdman LK,Lu Z,Serghides L,Zhong K,Dhabangi A,Musoke C,Gerard C,Cserti-Gazdewich C,Liles WC,Kain KC

    更新日期:2014-01-01 00:00:00

  • Induction of gamma interferon and nitric oxide by truncated pneumolysin that lacks pore-forming activity.

    abstract::Pneumolysin (PLY), an important virulence factor of Streptococcus pneumoniae, is known to exert various effects on the host immune cells, including cytokine induction, in addition to its known cytolytic activity as a member of the thiol-activated cytolysins. It is of interest to determine whether cytolytic activity is...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.1.107-113.2002

    authors: Baba H,Kawamura I,Kohda C,Nomura T,Ito Y,Kimoto T,Watanabe I,Ichiyama S,Mitsuyama M

    更新日期:2002-01-01 00:00:00

  • Three-dimensional reconstruction of Coxiella burnetii-infected L929 cells by high-voltage electron microscopy.

    abstract::Previous examination of thin sections of L929 cells heavily infected with the Q fever Priscilla isolate by conventional transmission electron microscopy indicated that the rickettsiae resided within multiple vacuoles. The present study using high-voltage electron microscopy and three-dimensional reconstruction reveale...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.10.4485-4488.1993

    authors: Hechemy KE,McKee M,Marko M,Samsonoff WA,Roman M,Baca O

    更新日期:1993-10-01 00:00:00

  • Complement receptor 1 expression on mouse erythrocytes mediates clearance of Streptococcus pneumoniae by immune adherence.

    abstract::Complement-containing immune complexes can be presented to phagocytes by human erythrocytes bearing complement receptor 1 (CR1). Although this has long been assumed to be a mechanism by which humans are able to protect themselves from "extracellular" bacteria such as pneumococci, there is little direct evidence. In th...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01263-09

    authors: Li J,Wang JP,Ghiran I,Cerny A,Szalai AJ,Briles DE,Finberg RW

    更新日期:2010-07-01 00:00:00

  • Role of Vibrio cholerae neuraminidase in the function of cholera toxin.

    abstract::Vibrio cholerae neuraminidase (NANase) is hypothesized to act synergistically with cholera toxin (CT) and increase the severity of a secretory response by increasing the binding and penetration of CT to enterocytes. To test this hypothesis, the NANase gene (nanH) from V. cholerae Ogawa 395 was first cloned and sequenc...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.2.406-415.1992

    authors: Galen JE,Ketley JM,Fasano A,Richardson SH,Wasserman SS,Kaper JB

    更新日期:1992-02-01 00:00:00

  • Comparison of irradiated-cercaria schistosome vaccine models that use 15- and 50-kilorad doses: the 15-kilorad dose gives greater protection, smaller liver sizes, and higher gamma interferon levels after challenge.

    abstract::The protection and immune response to infection caused by the parasite Schistosoma mansoni were studied by comparison of two murine irradiated-vaccine models. Mice were exposed from 1 to 4 times to infective-stage cercariae attenuated with either a moderate dose (15 kilorads) or a high dose (50 kilorads) of radiation....

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.1.90-94.1992

    authors: Reynolds SR,Harn DA

    更新日期:1992-01-01 00:00:00

  • The MIC3 gene of Toxoplasma gondii is a novel potent vaccine candidate against toxoplasmosis.

    abstract::Infection with the intracellular protozoan parasite Toxoplasma gondii causes serious public health problems and is of great economic importance worldwide. The micronemal protein MIC3, which is a potent adhesin of T. gondii, could be a significant candidate vaccine against toxoplasmosis. In this study, all CBA/J mice i...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.11.6222-6228.2003

    authors: Ismael AB,Sekkai D,Collin C,Bout D,Mévélec MN

    更新日期:2003-11-01 00:00:00

  • A novel component different from endotoxin extracted from Prevotella intermedia ATCC 25611 activates lymphoid cells from C3H/HeJ mice and gingival fibroblasts from humans.

    abstract::A novel immunobiologically active fraction was prepared from a phenol-water extract of Prevotella intermedia ATCC 25611 by Sephadex G-100 column chromatography. The fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. The fraction showed high-molecular-weight bands (10,000 to 12,000) on ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.11.4531-4538.1997

    authors: Iki K,Kawahara K,Sawamura S,Arakaki R,Sakuta T,Sugiyama A,Tamura H,Sueda T,Hamada S,Takada H

    更新日期:1997-11-01 00:00:00

  • Morphology, Physiology, and Virulence of Some Mutants of Candida albicans.

    abstract::Five mutagens were used to induce mutants of Candida albicans. Ultraviolet light, N-nitroso-N'-methyl-N-nitrosoguanidine, and N-nitroso-N-methylurethane were effective mutagens which induced stable auxotrophs. N-nitroso-N-methylurethane produced the largest number and variety of mutants. Nitrous acid and hydroxylamine...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.3.1.141-148.1971

    authors: Savage N,Balish E

    更新日期:1971-01-01 00:00:00

  • Surface-expressed mig protein protects Streptococcus dysgalactiae against phagocytosis by bovine neutrophils.

    abstract::The mig gene of Streptococcus dysgalactiae, a major bovine mastitis pathogen, encodes two plasma protein-binding receptors, alpha2-macroglobulin (alpha2-M) and immunoglobulin G (IgG). In this study, the mig gene from one S. dysgalactiae isolate was cloned and expressed in Escherichia coli. The IgG receptor region enco...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.10.6030-6037.2001

    authors: Song XM,Perez-Casal J,Bolton A,Potter AA

    更新日期:2001-10-01 00:00:00

  • Chlamydial pneumonitis induced in newborn guinea pigs.

    abstract::One- to three-day-old guinea pigs were inoculated intranasally with the chlamydial agent of guinea pig inclusion conjunctivitis. Physical signs of infection included a marked increase in respiration rate on days 5 to 10 of infection and radiographic evidence of pneumonia on day 6. When animals were killed at various t...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.1.153-158.1985

    authors: Rank RG,Hough AJ Jr,Jacobs RF,Cohen C,Barron AL

    更新日期:1985-04-01 00:00:00

  • Comparable endotoxic properties of lipopolysaccharides are manifest in diverse clinical isolates of gram-negative bacteria.

    abstract::In general there is a poor correlation between serum lipopolysaccharide (LPS; the biologically active constituent of endotoxin) levels and mortality in septic patients. The objective of this study was to determine if chemical, structural, or biological differences among LPS from different clinical isolates of gram-neg...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.4.1899-1904.2000

    authors: Luchi M,Morrison DC

    更新日期:2000-04-01 00:00:00

  • Competitive science: is competition ruining science?

    abstract::Science has always been a competitive undertaking. Despite recognition of the benefits of cooperation and team science, reduced availability of funding and jobs has made science more competitive than ever. Here we consider the benefits of competition in providing incentives to scientists and the adverse effects of com...

    journal_title:Infection and immunity

    pub_type: 社论

    doi:10.1128/IAI.02939-14

    authors: Fang FC,Casadevall A

    更新日期:2015-04-01 00:00:00

  • Cell-mediated immunity to varicella-zoster virus demonstrated by viral inactivation with human leukocytes.

    abstract::Cell-mediated immunity to varicella-zoster (V-Z) virus in persons immune to varicella has been demonstrated, using a tissue culture technique. Cell-mediated immunity was reflected by the ability of peripheral leukocytes (lymphocytes and monocytes) from human donors to inactivate V-Z virus. Leukocytes were stimulated b...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.13.6.1549-1553.1976

    authors: Gershon AA,Steinberg S,Smith M

    更新日期:1976-06-01 00:00:00

  • Isoprinosine augmentation of phytohemagglutinin-induced lymphocyte proliferation.

    abstract::The need for agents designed to modify immune response in the treatment of patients with viral infection, immunodeficiency, or cancer prompted the present study on the mechanisms of action of isoprinosine, a compound developed for antiviral use and whose therapeutic activity may involve the immune system. The effect o...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.13.2.382-387.1976

    authors: Hadden JW,Hadden EM,Coffey RG

    更新日期:1976-02-01 00:00:00

  • Analysis of a heme-dependent signal transduction system in Corynebacterium diphtheriae: deletion of the chrAS genes results in heme sensitivity and diminished heme-dependent activation of the hmuO promoter.

    abstract::The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is involved in the utilization of heme as an iron source. Transcription of hmuO is activated by heme or hemoglobin and repressed by iron and DtxR. Previous studies with Escherichia coli showed that heme-dependent transcriptional activation of an h...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.11.7406-7412.2005

    authors: Bibb LA,King ND,Kunkle CA,Schmitt MP

    更新日期:2005-11-01 00:00:00

  • The life cycle stages of Pneumocystis murina have opposing effects on the immune response to this opportunistic, fungal pathogen.

    abstract::The cyst cell wall β-glucans of Pneumocystis have been shown to stimulate immune responses in lung epithelial cells, dendritic cells, and alveolar macrophages. Little is known about how the trophic life forms, which do not have a fungal cell wall, interact with these innate immune cells. Here, we report differences in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00519-16

    authors: Evans HM,Bryant GL 3rd,Garvy BA

    更新日期:2016-11-01 00:00:00

  • Comparative immunogenicity of group 6 pneumococcal type 6A(6) and type 6B(26) capsular polysaccharides.

    abstract::The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera from adult volunteers injected with polyvalent pneumococcal vaccines containing either 50 mug of type 6A (U.S. designatio...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.26.3.1116-1122.1979

    authors: Robbins JB,Lee CJ,Rastogi SC,Schiffman G,Henrichsen J

    更新日期:1979-12-01 00:00:00

  • Neutralization of lymphocyte immortalization by different strains of Epstein-Barr virus with a murine monoclonal antibody.

    abstract::A murine monoclonal antibody was raised against the B95-8 strain of Epstein-Barr virus (EBV), which was isolated from a case of mononucleosis after blood transfusion (Hoffman et al. Proc. Natl. Acad. Sci. U.S.A. 77:2979, 1980). We provide evidence that neutralization of immortalization by this monoclonal antibody is v...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.3.1028-1031.1982

    authors: Miller G,Heston L,Hoffman G

    更新日期:1982-09-01 00:00:00

  • Group B streptococci and other gram-positive cocci bind to cytokeratin 8.

    abstract::Group B streptococci (GBS) adhere to surface receptors present on epithelial cells; these receptors include fibronectin and laminin. To identify other possible receptors, plasma membranes from A549 cells, a respiratory tract epithelial cell line, were prepared. These plasma membranes were tested in a protein blot anal...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.4.2129-2134.2000

    authors: Tamura GS,Nittayajarn A

    更新日期:2000-04-01 00:00:00

  • Use of auxotyping for epidemiological studies of Campylobacter jejuni and Campylobacter coli infections.

    abstract::A chemically defined medium developed for Neisseria gonorrhoeae was modified to support the growth of Campylobacter jejuni and Campylobacter coli. A total of 76 isolates of C. jejuni and 14 isolates of C. coli were tested on this medium, which was designated Campylobacter defined medium (CDM), over a 3-month period. A...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.2.384-388.1985

    authors: Tenover FC,Knapp JS,Patton C,Plorde JJ

    更新日期:1985-05-01 00:00:00

  • Iron limitation and the gamma interferon-mediated antihistoplasma state of murine macrophages.

    abstract::The zoopathogenic fungus Histoplasma capsulatum requires iron for growth. Intracellular growth of the fungus within mouse peritoneal macrophages is inhibited by recombinant murine gamma interferon (IFN-gamma). Such treatment of mouse peritoneal macrophages induces a marked downshift in transferrin receptors. We tested...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.7.2274-2278.1991

    authors: Lane TE,Wu-Hsieh BA,Howard DH

    更新日期:1991-07-01 00:00:00

  • Novel adjuvant action of lipopolysaccharides that possess mannose homopolysaccharides as O-specific polysaccharides on immune responses to nonimmunogenic autoantigens in mice.

    abstract::The adjuvant action of various lipopolysaccharides on immune responses to syngeneic tissue extract in mice was examined. Only lipopolysaccharides possessing the linear mannose homopolysaccharides as O-specific polysaccharides exhibited definite adjuvant action on immune responses to the autoantigens. The intensity of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.11.4953-4956.1992

    authors: Yokochi T,Fukada M,Kawai M,Zhang YH,Jiang GZ,Takahashi K

    更新日期:1992-11-01 00:00:00

  • Humanized mice, a new model to study the influence of drug treatment on neonatal sepsis.

    abstract::Bacterial infection with group B Streptococcus (GBS) represents a prominent threat to neonates and fetuses in the Western world, causing severe organ damage and even death. To improve current therapeutic strategies and to investigate new approaches, an appropriate in vivo model to study the immune response of a human ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01235-12

    authors: Ernst W,Zimara N,Hanses F,Männel DN,Seelbach-Göbel B,Wege AK

    更新日期:2013-05-01 00:00:00

  • Discordant Brucella melitensis antigens yield cognate CD8+ T cells in vivo.

    abstract::Brucella spp. are intracellular bacteria that cause the most frequent zoonosis in the world. Although recent work has advanced the field of Brucella vaccine development, there remains no safe human vaccine. In order to produce a safe and effective human vaccine, the immune response to Brucella spp. requires greater un...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00994-09

    authors: Durward MA,Harms J,Magnani DM,Eskra L,Splitter GA

    更新日期:2010-01-01 00:00:00