Abstract:
:We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5alpha and ChBac5, to detailed analysis. OaBac5alpha and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. While the peptides remained active against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Listeria monocytogenes in 100 mM NaCl, they lost activity against Staphylococcus aureus and Candida albicans under these conditions. ChBac5 was shown to bind lipopolysaccharide, a property that could enhance its ability to kill gram-negative bacteria. Proline-rich Bac5 peptides are highly conserved in ruminants and may contribute significantly to their innate host defense mechanisms.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Shamova O,Brogden KA,Zhao C,Nguyen T,Kokryakov VN,Lehrer RIdoi
10.1128/IAI.67.8.4106-4111.1999keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
4106-11issue
8eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from hors...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2776-2782.1999
更新日期:1999-06-01 00:00:00
abstract::Leishmaniae are protozoans which, depending upon both the host and parasite species, can cause either a healing or nonhealing infection. While C57BL/10 mice are able to heal following infection with Leishmania major, they fail to heal following infection with Leishmania amazonensis. In order to address the role of Th1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.7.2952-2959.1993
更新日期:1993-07-01 00:00:00
abstract::We examined the association of Herpesvirus saimiri with lymphocyte subsets and the functional integrity and distribution of these populations in owl monkeys with chronic, disease-free infections, in uninoculated, control animals, and in one monkey with H. saimiri-induced lymphoma. The lymphocyte subpopulations examine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.698-706.1981
更新日期:1981-05-01 00:00:00
abstract::Borrelia burgdorferi, the causative agent of Lyme disease, has a complex genome consisting of a linear chromosome and up to 21 linear and circular plasmids. These plasmids encode numerous proteins critical to the spirochete's infectious cycle and many hypothetical proteins whose functions and requirements are unknown....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01059-09
更新日期:2010-06-01 00:00:00
abstract::Cryptococcus neoformans is surrounded by an antiphagocytic polysaccharide capsule whose primary constituent is glucuronoxylomannan (GXM). Three prominent structural features of GXM are single xylosyl and glucuronosyl side chains and O acetylation of the mannose backbone. Isogenic pairs of O-acetyl-positive and O-acety...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2868-2875.2003
更新日期:2003-05-01 00:00:00
abstract::Molecular approaches to the rapid analysis of the serotyping antigens of Neisseria meningitidis, the class 2 and 3 outer membrane proteins (OMPs), were developed, evaluated, and used to study 12 antigenic variants of these proteins. A primer set for the polymerase chain reaction (PCR) amplification of the genes encodi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3620-3629.1992
更新日期:1992-09-01 00:00:00
abstract::The immunogenicity of the two major group 19 pneumococcal polysaccharides, types 19F and 19A, was studied in human volunteers. Antibody responses after vaccination with either 50 micrograms of type 19F polysaccharide, 50 micrograms of type 19A polysaccharide, or 25 micrograms of both polysaccharides premixed in a sing...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
doi:10.1128/IAI.36.3.1261-1262.1982
更新日期:1982-06-01 00:00:00
abstract::Although protective immunity in C57BL/6 mice induced by a single dose of the radiation-attenuated schistosome vaccine is believed to be mediated by Th1-type immune responses, we here report that in BALB/c mice protection can also depend upon signaling via the interleukin-4 (IL-4) receptor which conventionally governs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.228-236.2001
更新日期:2001-01-01 00:00:00
abstract::Human gamma delta T cells are known to respond at high frequencies to pathogenic mycobacteria. Here we show that opportunistic strains of mycobacteria share with pathogenic mycobacteria the ability to trigger at high frequencies human V gamma 9V delta 2 T-cell-receptor-positive T lymphocytes. Stimulating ligands were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2753-2757.1992
更新日期:1992-07-01 00:00:00
abstract::Specific stimulation of T cells by phytohemagglutinin and Lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (CFPE) derived from bacterial cultures of Mycobacterium tuberculosis H37Ra (avirulent) and H37Rv (virulent). The induct...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.309-313.1986
更新日期:1986-04-01 00:00:00
abstract::Outer membrane protein F (porin) was purified by extraction from polyacrylamide gels of cell envelope proteins of the Pseudomonas aeruginosa PAO1 strain. Rats were immunized intramuscularly with 25 micrograms of protein F on days 1 and 14 and then challenged on day 28 via intratracheal inoculation of bacterium-contain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1017-1022.1988
更新日期:1988-05-01 00:00:00
abstract::The lipooligosaccharide (LOS) present in the outer membrane of Haemophilus ducreyi is likely a virulence factor for this sexually transmitted pathogen. An open reading frame in H. ducreyi 35000 was found to encode a predicted protein that had 87% identity with the protein product of the gmhA (isn) gene of Haemophilus ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Aeromonas salmonicida causes furunculosis in salmonids and is a threat to Atlantic salmon aquaculture. The epithelial surfaces that the pathogen colonizes are covered by a mucus layer predominantly comprised of secreted mucins. By using mass spectrometry to identify mucin glycan structures with and without enzymatic r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00189-17
更新日期:2017-07-19 00:00:00
abstract::The adenylate cyclase-hemolysin of Bordetella pertussis consists of a cell-invasive N-terminal adenylate cyclase domain linked to a C-terminal RTX hemolysin containing extensive glycine-rich repeats. The toxin is an essential virulence factor required in the initial stages of infection. Adenylate cyclase-hemolysin was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3309-3315.1995
更新日期:1995-09-01 00:00:00
abstract::The Leishmania pifanoi amastigote antigen P-8 has been previously shown to induce protective immunity in a murine model of cutaneous leishmaniasis (L. Soong, S. M. Duboise, P. Kima, and D. McMahon-Pratt, Infect. Immun. 63:3559-3566, 1995). As this antigen is of interest for further vaccine studies, the biochemical cha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.6776-6784.2001
更新日期:2001-11-01 00:00:00
abstract::The effects of heat and chemical inactivation on the antigenicity and immunogenicity of Vibrio cholerae 1418 in rabbits were studied. V. cholerae 1418 was inactivated with heat and chemical inactivants (phenol or Formalin) alone or in combination. Enzyme-linked immunoassay systems employing whole cells of V. cholerae ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.21-26.1982
更新日期:1982-10-01 00:00:00
abstract::Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with acrylamide copolymerized with gelatin (substrate-SDS-PAGE) were combined to evaluate the proteinases of both long-term-grown and fresh isolates of Trichomonas vaginalis. This two-dimensional substrate-SDS-PAGE resolved a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.157-162.1990
更新日期:1990-01-01 00:00:00
abstract::Evidence obtained from gene knockout studies supports the role of Toll-like receptor 4 (TLR4) in intestinal inflammation and microbiota recognition. Increased epithelial TLR4 expression is observed in patients with inflammatory bowel disease. However, little is known of the effect of increased TLR4 signaling on intest...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01374-15
更新日期:2016-01-11 00:00:00
abstract::Thirty-one Kanagawa-positive and -negative Vibrio parahaemolyticus isolates from patient stools and seafood were examined for plasmid deoxyribonucleic acid content, culture characteristics, and indices of pathogenicity. No significant correlation was found between plasmid deoxyribonucleic acid contents and indices of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.322-325.1981
更新日期:1981-07-01 00:00:00
abstract::Antibody responses against proteins located on the surface or in the apical organelles of merozoites are presumed to be important components of naturally acquired protective immune responses against the malaria parasite Plasmodium falciparum. However, many merozoite antigens are highly polymorphic, and antibodies indu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.422-430.2005
更新日期:2005-01-01 00:00:00
abstract::In contrast to infection of superficial tissues, Streptococcus pyogenes infection of deeper tissue can be associated with a significantly diminished inflammatory response, suggesting that this bacterium has the ability to both promote and suppress inflammation. To examine this, we analyzed the behavior of an S. pyogen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00420-09
更新日期:2009-11-01 00:00:00
abstract::Thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in HeLa 229 cells pretreated with diethylaminoethyl-dextran to determine the relative sensitivity of the two culture systems for detection of the infection. The strains tested were both yolk sac- and HeLa cell-esta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.5.1078-1082.1975
更新日期:1975-11-01 00:00:00
abstract::The membrane-damaging properties on human diploid embryonic lung fibroblasts of streptolysin O (from Streptococcus pyogenes) and theta-toxin (from Clostridium perfringens) were compared. The results are consistent with the suggested mechanism for hemolysis by streptolysin O involving one fixation site and one lytic si...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::Existing evidence suggests that oxidative insults and antioxidant defense mechanisms play a critical role in the host cell response during infection of endothelial cells by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever. Heme oxygenase (HO), a rate-limiting enzyme in the pathway for heme ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4045-4052.2002
更新日期:2002-08-01 00:00:00
abstract::Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.751-755.1988
更新日期:1988-04-01 00:00:00
abstract::To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.297-301.1980
更新日期:1980-02-01 00:00:00
abstract::Escherichia coli isolates from patients with bacteriuria of pregnancy were compared by PCR with isolates from patients with community-acquired cystitis for the presence of established virulence determinants. The strains from patients with bacteriuria of pregnancy were less likely to carry genes for P-family, S-family,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.794-799.2001
更新日期:2001-02-01 00:00:00
abstract::Cytomegalovirus (CMV) infection is common after renal transplantation, and cytomegaloviruria occurs in about two-thirds of the recipients. These observations suggest that the allografts may be a site of latent infection with CMV and its reactivation may be the source of viruria. To investigate this possibility, 130 ki...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.699-703.1978
更新日期:1978-02-01 00:00:00
abstract::The interaction of purified human fibrinogen with Aspergillus fumigatus conidia was investigated by immunofluorescence and electron microscopy and binding assays with radiolabeled proteins. We described the localization of the binding sites on the A. fumigatus conidia and on the fibrinogen molecule and determined the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1747-1755.1992
更新日期:1992-05-01 00:00:00
abstract::Legionella pneumophila is ingested by both human macrophages and amoebae, and it multiplies within similar endocytic compartments in both eukaryotic species. Inhibitors of eukaryotic protein synthesis, such as cycloheximide and emetine, had no effect on the uptake of L. pneumophila by macrophages but completely abolis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1860-1866.1994
更新日期:1994-05-01 00:00:00