Abstract:
:The interaction of purified human fibrinogen with Aspergillus fumigatus conidia was investigated by immunofluorescence and electron microscopy and binding assays with radiolabeled proteins. We described the localization of the binding sites on the A. fumigatus conidia and on the fibrinogen molecule and determined the binding characteristics. Immunofluorescence revealed that the fixation of purified fibrinogen was selectively associated with conidia and suggested a role for the D domains of the fibrinogen molecule. Binding assays performed with 125I-radiolabeled proteins confirmed that binding sites were located specifically in the D domains. No reaction could be detected with fragment E. The binding of 125I-fragment D to conidia was time dependent, saturable, and specific. Scatchard analysis of the data revealed an average of 1,200 binding sites per conidium, and an apparent dissociation constant (Kd) of 2.2 x 10(-9) M was estimated. Pretreatment of the cells with proteolytic enzymes or heat abolished binding, demonstrating the protein nature of the binding sites. Ultrastructural localization of the fungal receptors was determined by transmission electron microscopy. Labeling appeared to be associated with the outer electron-dense layer of the conidial wall and progressively decreased during the germination process. Labeling of thin sections with fragment D and an antifibrinogen immune serum revealed that binding sites also lay in the inner part of the wall and in vacuoles. These results indicate the presence at the conidial surface of specific receptors for fibrinogen which could act as mediators of conidial adherence to host tissues.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Annaix V,Bouchara JP,Larcher G,Chabasse D,Tronchin Gdoi
10.1128/IAI.60.5.1747-1755.1992keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
1747-55issue
5eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::The objective of this research was to determine whether gram-negative bacteria frequently isolated from periodontally diseased sites contained polyclonal B-cell activators. Polyclonal B-cell activation, which results in nonspecific activation of multiple B-cell clones was analyzed by a hemolysis-in-gel assay designed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.43-49.1981
更新日期:1981-10-01 00:00:00
abstract::The lymphogranuloma venereum (LGV) and trachoma biovars of Chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. To identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (OMPs) by means of isoelectric focusing and none...
journal_title:Infection and immunity
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doi:10.1128/IAI.50.2.488-494.1985
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.647-653.1985
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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doi:10.1128/IAI.57.11.3364-3371.1989
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.1273-1278.1982
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.297-301.1980
更新日期:1980-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1098-1108.2003
更新日期:2003-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.6.1315-1321.1976
更新日期:1976-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2010-09-01 00:00:00
abstract::Trypanosome-activated mouse peritoneal macrophages phagocytized and digested Trypanosoma brucei in vitro and in vivo, but in the absence of specific antiserum and complement the degree of phagocytosis was minimal. Ultrastructurally, the parasites attached to the macrophage by their flagella, and ingestion proceeded fl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.972-982.1978
更新日期:1978-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00699-12
更新日期:2012-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5669-5676.1998
更新日期:1998-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.155-165.2005
更新日期:2005-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00700-09
更新日期:2009-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.716-722.1981
更新日期:1981-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.56.8.1897-1900.1988
更新日期:1988-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.90-94.1992
更新日期:1992-01-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.629-632.1983
更新日期:1983-05-01 00:00:00
abstract::Both rat alveolar macrophages and a human macrophages cell line with characteristics of human tissue (e.g., alveolar) macrophages (THP-1) were found to inhibit the germination of Rhizopus spores. However, the conditions under which fungistatic activity occurs are different for these two cell types. The inhibition of R...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4489-4494.1995
更新日期:1995-11-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular parasite that resides in the cytoplasm of its host in a unique membrane-bound vacuole known as the parasitophorous vacuole (PV). The membrane surrounding the parasite is remodeled by the dense granules, secretory organelles that release an array of proteins into the vacuo...
journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5329-5336.1998
更新日期:1998-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2766-2777.2005
更新日期:2005-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-08-01 00:00:00