Abstract:
:The selection of influenza B virus recombinants from plaques in bovine kidney cell monolayers is described. Two sets of recombinants were each derived from parents of high and low virulence for humans, respectively. Recombination frequency was apparently high, and reassortment of genes made it possible to obtain attenuated recombinants containing the surface antigens of the virulent parents. Attenuation and immunogenicity were demonstrated in a series of volunteer trials. However, technique proved less satisfactory than for influenza A viruses which periodically undergo antigenic shift and for which there is a wide choice of parent viruses with distinctive surface antigens. In our two influenza B recombinant series there was appreciable antigenic overlap in the neuraminidases of the parents, even though in both cases these were chronologically widely separated. Another marker used was comparative titer at 35 and 38 degrees C. In practice, technical problems might sometimes make it difficult to ensure rapid production of live influenza B vaccines by recombination.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Beare AS,Sherwood JE,Callow KA,Craig JWdoi
10.1128/IAI.15.2.347-353.1977subject
Has Abstractpub_date
1977-02-01 00:00:00pages
347-53issue
2eissn
0019-9567issn
1098-5522journal_volume
15pub_type
杂志文章abstract::Bile aspirated from chicken gall bladders was found to contain substances neutralizing Newcastle disease virus (NDV). Nonspecific factors were present in the bile, probably the bile acids, which caused a reduction in the infectivity of the virus. Specific anti-NDV activity was found in the bile of birds that had been ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.692-697.1975
更新日期:1975-04-01 00:00:00
abstract::We developed an efficient electrotransformation system for the pathogenic fungus Histoplasma capsulatum and used it to examine the effects of features of the transforming DNA on transformation efficiency and fate of the transforming DNA and to demonstrate fungal expression of two recombinant Escherichia coli genes, hp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1697-1707.1998
更新日期:1998-04-01 00:00:00
abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal pneumonia, sepsis, and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes 35 proteins containing an LPXTG motif which are thought to be covalently linked to the peptidoglycan by an enzyme called sorta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3342-3350.2005
更新日期:2005-06-01 00:00:00
abstract::We tested the hypothesis that the intensity of specific antichlamydial T cell-mediated immunity in the genital tract of female guinea pigs infected intravaginally with the chlamydial agent of guinea pig inclusion conjunctivitis would determine the resistance or susceptibility to reinfection after a primary chlamydial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1346-1351.1991
更新日期:1991-04-01 00:00:00
abstract::The role of CD4+ and CD8+ T cells in the response to intranasal infection with a Mycobacterium avium complex isolate (MAC) was investigated. Depletion of CD4+ T cells by injected antibody exacerbated infection in the lung, spleen, and liver. There were decreased numbers of inflammatory cells in the lungs of CD4-deplet...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.6.2282-2287.1995
更新日期:1995-06-01 00:00:00
abstract::Hydrogen peroxide (H2O2) is a by-product of glycerol metabolism in mycoplasmas and has been shown to cause cytotoxicity for cocultured eukaryotic cells. There appears to be selective pressure for mycoplasmas to retain the genes needed for glycerol metabolism. This has generated interest and speculation as to their fun...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02208-14
更新日期:2014-12-01 00:00:00
abstract::Staphylococcus aureus fibronectin-binding proteins (FnBPs) play a critical role in S. aureus pathogenesis. FnBPs mediate adhesion to fibronectin and invasion of mammalian cells, including epithelial, endothelial, and fibroblastic cells, by fibronectin bridging to the host cell fibronectin receptor integrin (alpha(5))b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7155-7163.2004
更新日期:2004-12-01 00:00:00
abstract::Chronic lung infections with Pseudomonas aeruginosa biofilms are associated with refractory and fatal pneumonia in cystic fibrosis (CF). In this study, a group of genomically diverse P. aeruginosa isolates were compared with the reference strain PAO1 to assess the roles of motility, twitching, growth rate, and overpro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.133-144.2004
更新日期:2004-01-01 00:00:00
abstract::In vitro studies have shown that enterotoxigenic Escherichia coli (ETEC) strains are capable of invading cultured epithelial cells derived from the human ileum and colon. Two separate invasion loci (tia and tib) have previously been isolated from the classical ETEC strain H10407. The tia locus has been shown to direct...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6595-6601.2000
更新日期:2000-12-01 00:00:00
abstract::Bordetella bronchiseptica secreted a hydroxamate siderophore when grown in Fe-depleted medium. A Tn5lac insertion mutant of B. bronchiseptica, DBB22, did not produce this hydroxamate siderophore and was incapable of using lactoferrin as an Fe source. Our data suggest that B. bronchiseptica uses a siderophore for remov...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2698-2702.1993
更新日期:1993-06-01 00:00:00
abstract::Chronic infections play a significant role in the morbidity and mortality of patients with chronic airflow limitation. By stimulating airway inflammation, persistent infection has the potential to cause airway fibrosis. However, in patient this condition is most typically found in lungs damaged by other factors, such ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2936-2942.1992
更新日期:1992-07-01 00:00:00
abstract::The in vivo role of endogenous interleukin 12 (IL-12) in modulating intrapulmonary growth of Legionella pneumophila was assessed by using a murine model of replicative L. pneumophila lung infection. Intratracheal inoculation of A/J mice with virulent bacteria (10(6) L. pneumophila cells per mouse) resulted in inductio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.65-69.1998
更新日期:1998-01-01 00:00:00
abstract::The fibronectin-binding proteins (FnBPs) of Staphylococcus aureus are believed to be implicated in the pathogen's adherence to and colonization of bovine mammary glands, thus leading to infectious mastitis. In vitro studies have shown that FnBPs help the adhesion of the pathogen to bovine mammary epithelial cells. How...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2292-2295.2003
更新日期:2003-04-01 00:00:00
abstract::A receptor moiety and blood group substance recognized by the O75X adhesin was studied. Well-defined erythrocytes representing different blood group systems and bacterial derivatives carrying plasmid pBJN406 encoding the adhesin were used in a direct hemagglutination assay. We showed that Dr blood group antigen, a com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1057-1060.1988
更新日期:1988-05-01 00:00:00
abstract::Monolayer cultures of LLC-MK2 rhesus monkey kidney cells become persistently infected with simian virus 40 after infection at input multiplicities of 100, 10, or 1 plaque-forming unit per cell. After 3 weeks, all cells of the cultures infected at a multiplicity of 1 plaque-forming unit per cell produced the simian vir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.868-871.1977
更新日期:1977-12-01 00:00:00
abstract::Previous reports have demonstrated that heat-stable (A-type) capsule on piliated enterotoxigenic Escherichia coli enhances colonization of enterotoxigenic E. coli in the small intestine and enhances virulence of enterotoxigenic E. coli. In this report, four encapsulated enterotoxigenic E. coli strains and one encapsul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.737-740.1984
更新日期:1984-09-01 00:00:00
abstract::An enzyme from Treponema denticola that hydrolyzes a synthetic trypsin substrate, N-alpha-benzoyl-L-arginine-p-nitroanilide (BAPNA), was purified to near homogeneity, as judged by gel electrophoresis. The molecular weight of the enzyme was estimated to be ca. 69,000 by sodium dodecyl sulfate-polyacrylamide gel electro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.213-220.1986
更新日期:1986-07-01 00:00:00
abstract::We reexamined the binding specificity of the Shiga-like toxin variant associated with porcine edema disease, SLT2e, which is reported to be more cytotoxic for Vero cells than for HeLa cells, by using receptor-deficient cells and a liposomal insertion system for purified glycolipids. We found that SLT2e preferentially ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.1138-1141.1995
更新日期:1995-03-01 00:00:00
abstract::We investigated the cytokine/chemokine secretions and alteration of protein expression from peripheral blood mononuclear cells (PBMCs) cocultured with adult liver flukes (Opisthorchis viverrini) for 6 to 24 h. PBMC-derived proteins were identified by two-dimensional electrophoresis and mass spectrometry, and the cytok...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00901-13
更新日期:2014-05-01 00:00:00
abstract::Protective immunity against Babesia bigemina is hypothesized to involve antibodies directed against merozoite surface-exposed epitopes. Levels of antibody against a rhoptry-associated protein 1 (RAP-1) B-lymphocyte epitope, defined by surface-reactive and inhibitory monoclonal antibodies, in immune cattle sera were de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5698-5701.1994
更新日期:1994-12-01 00:00:00
abstract::We used inhibitors of receptor-mediated endocytosis to study the mechanisms of infectivity, especially the uptake mechanism, of Chlamydia trachomatis for cultured cells. The effect of methylamine and monodansylcadaverine on the different stages of the chlamydial growth cycle in McCoy cells was examined. There was a do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.534-541.1983
更新日期:1983-05-01 00:00:00
abstract::In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 microg of Vi and induced a greater-than-eightfold rise in immunoglobulin G (IgG) anti-Vi in all of the vaccinees tested. In this investigati...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.72.11.6586-6588.2004
更新日期:2004-11-01 00:00:00
abstract::The capacity of the intracellular pathogen Listeria monocytogenes to activate the alternative pathway of human complement was examined. Incubation of L. monocytogenes with human serum in optimal conditions (20% Mg2+EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid]-chelated serum) consumed (3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5134-5139.1993
更新日期:1993-12-01 00:00:00
abstract::Campylobacter jejuni is the leading cause of infectious gastroenteritis in industrialized nations. Its ability to enter and survive within nonphagocytic cells is thought to be very important for pathogenesis. However, little is known about the C. jejuni determinants that mediate these processes. Through an extensive t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00109-10
更新日期:2010-08-01 00:00:00
abstract::One aspect in a broad spectrum of possible mechanisms of cariostatic reactions of fluoride is its interaction with the metabolism of oral bacteria. Information on the mechanisms and kinetics of fluoride inhibition of essential enzymes of the glycolytic pathway of the relevant bacteria is lacking. In this work, the iso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1043-1047.1990
更新日期:1990-04-01 00:00:00
abstract::Glucose metabolism and respiration of Candida albicans were compared under conditions which permitted either maximal filamentous or maximal yeast growth. Changes in metabolism were monitored by comparing the quantities of ethanol produced, CO2 evolved, and oxygen consumed. Filamenting cultures produced more ethanol an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.1.119-127.1975
更新日期:1975-07-01 00:00:00
abstract::Twitching motility is a form of surface-associated bacterial movement mediated by type IV pili of Pseudomonas aeruginosa. Others have shown that pilT and pilU mutants, which are piliated but defective in twitching motility, display reduced cytotoxic capacity towards epithelial cells in vitro. Although these mutants ef...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5389-5393.2003
更新日期:2003-09-01 00:00:00
abstract::A recombinant 23-kDa protein (rBPI23) derived from human bactericidal/permeability-increasing protein (BPI) possesses potent endotoxin-neutralizing abilities in vitro and in vivo. Binding of rBPI23 to those endotoxins (lipopolysaccharides [LPSs]) encountered clinically would be a prerequisite for efficacy in decreasin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3564-3567.1994
更新日期:1994-08-01 00:00:00
abstract::Chlamydia trachomatis is a strict human pathogen producing infections that cause medically important chronic inflammatory diseases, such as blinding trachoma and tubal factor infertility. Isolates exist as serotypes that fall into distinct biologic and pathological groups corresponding to differences in infection tiss...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7063-7072.2004
更新日期:2004-12-01 00:00:00
abstract::The phenotypic and functional changes of glycolipid presented by CD1d(glycolipid/CD1d) specific Valpha14+ T cells in the liver of mice at early stages of bacterial infection were investigated. After Listeria monocytogenes infection or interleukin-12 (IL-12) treatment, alpha-galactosylceramide/CD1d tetramer-reactive (a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00311-06
更新日期:2006-10-01 00:00:00