Abstract:
:Chlamydia trachomatis is a strict human pathogen producing infections that cause medically important chronic inflammatory diseases, such as blinding trachoma and tubal factor infertility. Isolates exist as serotypes that fall into distinct biologic and pathological groups corresponding to differences in infection tissue tropism and invasion properties. Paradoxically, genome sequencing of several diverse strains has revealed a remarkable level of genomic synteny, suggesting that minor genetic differences determine the pathogen host- and tissue-specific infection characteristics. To better understand the genetic basis of chlamydial pathobiologic diversity, we performed comparative DNA-DNA microarray genomic hybridizations with all 15 C. trachomatis serovariants. We found there are few major genetic differences among the 15 serovars. An exception was the cytotoxin locus located in the plasticity zone, a region that exhibited significant polymorphisms among serovars. We therefore sequenced this region from all 15 serovars. The cytotoxin gene was interrupted by extensive mutations and deletions among the different serovars; however, three basic open reading frame motifs were discovered that correlated with noninvasive oculotropic, urogenitotropic, and invasive serovars. Of interest, only noninvasive genitotropic serovars possessed an intact N-terminal portion of the putative toxin gene. This region contains the UDP-glucose binding domain and the glycosyltransferase domain required for enzymatic activity of the clostridial toxin homologs, suggesting a role in urogenital infection or pathogenesis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Carlson JH,Hughes S,Hogan D,Cieplak G,Sturdevant DE,McClarty G,Caldwell HD,Belland RJdoi
10.1128/IAI.72.12.7063-7072.2004keywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
7063-72issue
12eissn
0019-9567issn
1098-5522pii
72/12/7063journal_volume
72pub_type
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journal_title:Infection and immunity
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doi:10.1128/IAI.22.3.908-918.1978
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.458-467.1989
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.869-878.1979
更新日期:1979-06-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1983-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.3.645-651.1976
更新日期:1976-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.267-272.1982
更新日期:1982-10-01 00:00:00
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更新日期:1981-03-01 00:00:00
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更新日期:2000-03-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1978-01-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1993-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1984-05-01 00:00:00
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