Abstract:
:Cells of Candida albicans strain WO-1 and related strains switch frequently and reversibly between a white-colony-forming unit (white phase) and a gray-colony-forming unit (opaque phase). Cells in the budding white phase exhibit the usual smooth round phenotype observed in other C. albicans strains, but cells in the budding opaque phase exhibit a unique elongate shape with surface pimples or protrusions. In this study, it was demonstrated that opaque cells formed hyphae at low to negligible levels in suspension cultures but could be induced to form hyphae at high levels when anchored to the chamber wall of a perfusion chamber or to a monolayer of human skin epithelial cells. Variability in the proportion of hyphae formed between experiments appeared to be due to variability between individual opaque clones. The hyphae formed by opaque cells were morphologically identical to hyphae formed by white cells (i.e., they were devoid of pimples or protrusions and exhibited the same shape and septal locations). They also did not stain with an opaque-specific antiserum which differentially stained opaque budding cells in a punctate fashion. However, when stimulated to form buds, opaque hyphae formed opaque-shaped daughter buds, demonstrating that although they are morphologically similar to hyphae formed by white cells, they are genetically opaque.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Anderson J,Cundiff L,Schnars B,Gao MX,Mackenzie I,Soll DRdoi
10.1128/IAI.57.2.458-467.1989subject
Has Abstractpub_date
1989-02-01 00:00:00pages
458-67issue
2eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::Pulmonary washings from rabbits were freed of cells and added to the monolayers of homologous alveolar macrophages (AM). At 1 h after incubation with the pulmonary washings, many more cells adhered to glass, spread out, and showed enhanced Nitro Blue Tetrazolium reduction. The maximal effect of the pulmonary washings ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.476-484.1978
更新日期:1978-05-01 00:00:00
abstract::Pneumonic tularemia is a life-threatening disease caused by inhalation of the highly infectious intracellular bacterium Francisella tularensis. The most serious form of the disease associated with the type A strains can be prevented in experimental animals through vaccination with the attenuated live vaccine strain (L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00605-10
更新日期:2011-04-01 00:00:00
abstract::Bordetella pertussis, the etiological agent of whooping cough, has the ability to modulate its phenotype in response to environmental conditions by using the BvgAS sensory transduction system which is encoded by the vir locus (now known as bvg). The BvgAS system is part of a large family of two-component sensory trans...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3780-3789.1995
更新日期:1995-10-01 00:00:00
abstract::Repeated immunizations of CF1 mice with irradiated noninfectious Trypanosoma cruzi trypomastigotes resulted in partial protection against infection with live parasites. It also induced a limited number of antibody species that were reactive in Western blots with trypomastigote but not with epimastigote or amastigote p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.826-830.1984
更新日期:1984-12-01 00:00:00
abstract::Sera from adult periodontitis patients were analyzed for antibody activity against surface antigens of Bacteroides gingivalis by immunoblotting analysis. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.832-834.1987
更新日期:1987-03-01 00:00:00
abstract::Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1436-1441.2006
更新日期:2006-02-01 00:00:00
abstract::In this work, apolipoprotein A-I (ApoA-I) was purified from pig sera. The responses of this protein after sterile inflammation and in animals infected with Actinobacillus pleuropneumoniae or Streptococcus suis were investigated. Decreases in the concentrations of ApoA-I, two to five times lower than the initial values...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.3184-3187.2005
更新日期:2005-05-01 00:00:00
abstract::The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is involved in the utilization of heme as an iron source. Transcription of hmuO is activated by heme or hemoglobin and repressed by iron and DtxR. Previous studies with Escherichia coli showed that heme-dependent transcriptional activation of an h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7406-7412.2005
更新日期:2005-11-01 00:00:00
abstract::Detailed structural studies were undertaken on five isolates of bovine rotavirus which showed variability in the migration patterns of their genome segments on electrophoresis in polyacrylamide gels. The individual genome segments of each isolate were characterized by partial digestion of terminally radiolabeled RNA w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.2.492-497.1982
更新日期:1982-05-01 00:00:00
abstract::Saccharomyces boulardii is a nonpathogenic yeast used in the treatment of Clostridium difficile diarrhea and colitis. We have reported that S. boulardii inhibits C. difficile toxin A enteritis in rats by releasing a 54-kDa protease which digests the toxin A molecule and its brush border membrane (BBM) receptor (I. Cas...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.302-307.1999
更新日期:1999-01-01 00:00:00
abstract::The 260-kDa galactose-inhibitable lectin of Entamoeba histolytica mediates binding of amebic trophozoites to purified colonic mucins and intestinal epithelial cells. Parenteral immunization of Lewis rats with immuno-affinity-purified lectin with Freund's adjuvant and then intra-Peyer's patch inoculation of lectin with...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.686-689.1995
更新日期:1995-02-01 00:00:00
abstract::Pasteurella multocida is the causative agent of fowl cholera in birds. In a previous study using signature-tagged mutagenesis, we identified a mutant, AL251, which was attenuated for virulence in mice and in the natural chicken host. Sequence analysis indicated that AL251 had an insertional inactivation of the gene wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3436-3443.2004
更新日期:2004-06-01 00:00:00
abstract::Mycoplasma arthritidis induces an acute to chronic arthritis in rodents. Arthritis induced in mice histologically resembles human rheumatoid arthritis and can be associated with lethal toxicity following systemic injection. The M. arthritidis mitogen (MAM) superantigen has long been implicated as having a role in path...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00667-08
更新日期:2008-11-01 00:00:00
abstract::Pili have been demonstrated to be the adhesins of Pseudomonas aeruginosa for mouse epidermal cells. The mechanisms of adhesion of P. aeruginosa to mouse epidermal cells was studied by using four mutants derived from a single strain: flagellated and piliated (F+P+), flagellated and nonpiliated (F+P-), nonflagellated an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1774-1778.1987
更新日期:1987-08-01 00:00:00
abstract::The adherence of Trichomonas vaginalis NYH 286 to host cells was evaluated by using monolayer cultures of HeLa and HEp-2 epithelial cells and human fibroblast cell lines. Saturation of sites on HeLa cells was achieved, yielding a maximal T. vaginalis NYH 286-to-cell ratio of two. The ability of radiolabeled NYH 286 to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.701-708.1985
更新日期:1985-12-01 00:00:00
abstract::Conventionally raised suckling mice were injected intragastrically with 10(5) spores of a Clostridium botulinum type A culture. Botulism was not observed, but 80% or more of mice challenged when 8 to 11 days old had botulinum toxin in the large intestine 3 days later. Mice younger than 7 days or older than 15 days wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.59-63.1978
更新日期:1978-07-01 00:00:00
abstract::The pathogenicities of three proteases from Serratia marcescens, two proteases from Pseudomonas aeruginosa, and one thermolysin from Bacillus stearothermophilus were examined. All proteases tested caused acute liquefactive necrosis of the cornea and descemetocele formation in guinea pig eyes after intrastromal injecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2509-2517.1987
更新日期:1987-10-01 00:00:00
abstract::An alkali-soluble water-soluble extract of Blastomyces dermatitidis yeast-phase cell walls was tested for its ability to elicit a response in lymphocytes isolated from the peripheral blood of Blastomyces-infected guinea pigs. Sequential preparations of the antigen were reproducible and specific in the in vitro lymphoc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.411-415.1978
更新日期:1978-02-01 00:00:00
abstract::T cells are important in systemic anticryptococcal defenses, but a role in controlling an initial pulmonary infection has not been demonstrated. A murine model with intratracheal inoculation was developed to study the acquisition and expression of pulmonary T cell-mediated immunity against Cryptococcus neoformans. Inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1423-1433.1991
更新日期:1991-04-01 00:00:00
abstract::The mechanism of complement-mediated killing of seven serum-sensitive Pseudomonas aeruginosa strains was examined. All seven strains were sensitive to the bactericidal activity of 20% pooled normal human serum (PNHS) containing magnesium EGTA, which blocks the classical complement pathway (CCP), or 20% PNHS preheated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.3.632-639.1988
更新日期:1988-03-01 00:00:00
abstract::Free-living amoebae of the genus Acanthamoeba can cause severe and chronic infections in humans, mainly localized in immune privileged sites, such as the brain and the eye. Monocytes/macrophages are thought to be involved in Acanthamoeba infections, but little is known about how these facultative parasites influence t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00345-16
更新日期:2016-09-19 00:00:00
abstract::Onchocerca volvulus, a human parasitic nematode, is the third leading cause of preventable blindness worldwide. This study describes the molecular cloning of a novel superoxide dismutase (SOD) from the parasite. This putative O. volvulus extracellular SOD (OvEcSOD) is 628 nucleotides (nt) long, including a 22-nt 5' sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.713-716.1994
更新日期:1994-02-01 00:00:00
abstract::Five mutagens were used to induce mutants of Candida albicans. Ultraviolet light, N-nitroso-N'-methyl-N-nitrosoguanidine, and N-nitroso-N-methylurethane were effective mutagens which induced stable auxotrophs. N-nitroso-N-methylurethane produced the largest number and variety of mutants. Nitrous acid and hydroxylamine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.1.141-148.1971
更新日期:1971-01-01 00:00:00
abstract::Peptide 9 (TDAYNQKLSERRAN) and peptide 10 (NATAEGRAINRRVE) represent surface-exposed epitopes of outer membrane protein F of Pseudomonas aeruginosa. Rats immunized with four intramuscular inoculations on days 0, 14, 28, and 42 with either peptide 9 or peptide 10 conjugated to keyhole limpet hemocyanin were afforded pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.6.2347-2351.1995
更新日期:1995-06-01 00:00:00
abstract::We developed an efficient electrotransformation system for the pathogenic fungus Histoplasma capsulatum and used it to examine the effects of features of the transforming DNA on transformation efficiency and fate of the transforming DNA and to demonstrate fungal expression of two recombinant Escherichia coli genes, hp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1697-1707.1998
更新日期:1998-04-01 00:00:00
abstract::The population structure of the Bacillus cereus group (52 strains of B. anthracis, B. cereus, and B. thuringiensis) was investigated by sequencing seven gene fragments (rpoB, gyrB, pycA, mdh, mbl, mutS, and plcR). Most of the strains were classifiable into two large subgroups in six housekeeping gene trees but not in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5253-5261.2004
更新日期:2004-09-01 00:00:00
abstract::Three antigenic determinants were revealed in H3 hemagglutinin of influenza A viruses isolated from 1968 to 1975. One of them was common for all viruses, and two others specified differences between the viruses possessing H3 hemagglutinin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.804-807.1979
更新日期:1979-06-01 00:00:00
abstract::Type II secretion (T2S) is one means by which Gram-negative pathogens secrete proteins into the extracellular milieu and/or host organisms. Based upon recent genome sequencing, it is clear that T2S is largely restricted to the Proteobacteria, occurring in many, but not all, genera in the Alphaproteobacteria, Betaprote...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00014-17
更新日期:2017-04-21 00:00:00
abstract::The rationale for the present study was to determine how different species of bacteria interact with cells of the human meninges in order to gain information that would have broad relevance to understanding aspects of the innate immune response in the brain. Neisseria lactamica is an occasional cause of meningitis in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00644-06
更新日期:2006-11-01 00:00:00
abstract::Although recent evidence suggests that fibronectin may be involved in the attachment of treponemes to mammalian cells, its possible role in promoting phagocytosis of Treponema pallidum has not been investigated. In the present study, we examined the antibody-independent interactions of fibronectin, C1q, and human poly...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.456-464.1986
更新日期:1986-11-01 00:00:00