Abstract:
:Five mutagens were used to induce mutants of Candida albicans. Ultraviolet light, N-nitroso-N'-methyl-N-nitrosoguanidine, and N-nitroso-N-methylurethane were effective mutagens which induced stable auxotrophs. N-nitroso-N-methylurethane produced the largest number and variety of mutants. Nitrous acid and hydroxylamine were ineffective as mutagens although they killed C. albicans. All mutagenic agents employed induced colonial variants, especially small colony forms. The morphology, physiology, and virulence of one methionine and two adenine auxotrophs was compared to that of the prototroph. The auxotrophs exhibited yeast-like morphology in complex media and had sugar fermentation patterns typical of C. albicans, and all were agglutinated by C. albicans antiserum. Chlamydospore production was absent in the nonpigmented adenine mutant, and the chlamydospores produced by the methionine auxotroph were distorted. Germ tubes were formed in human serum by the auxotrophs and prototroph. Virulence for mice was retained by all auxotrophs but generally at a reduced level. The methionine auxotroph, only slightly less virulent than the prototroph, was more virulent than a pigmented adenine mutant and a practically avirulent nonpigmented adenine auxotroph.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Savage N,Balish Edoi
10.1128/IAI.3.1.141-148.1971keywords:
subject
Has Abstractpub_date
1971-01-01 00:00:00pages
141-8issue
1eissn
0019-9567issn
1098-5522journal_volume
3pub_type
杂志文章abstract::It is proposed that binding of oral streptococci to saliva-coated hydroxylapatite (SHA) surfaces is a multifactorial process involving both specific and nonspecific receptors. In this context, specific binding is described as a high-affinity, saturable interaction between the cell and binding surface. Conversely, nons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.653-659.1984
更新日期:1984-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.2.621-627.1982
更新日期:1982-05-01 00:00:00
abstract::In this study, we report on a novel approach based on modification of the intestinal surface to prevent diarrhea caused by enterotoxigenic Escherichia coli (ETEC). The removable intestinal tie adult rabbit diarrhea (RITARD) model was used to test the efficacy of an enteric-coated protease preparation (Detach; Enzacor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3708-3714.1991
更新日期:1991-10-01 00:00:00
abstract::A comparison of viral-induced unresponsiveness of phytohemagglutinin (PHA)-induced deoxyribonucleic acid (DNA) synthesis of mouse lymphocytes was made by culturing the cells under identical conditions in the presence of HEPES buffer in a humid-air atmosphere. The degree of PHA-induced DNA synthesis was found to vary, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.3.438-444.1973
更新日期:1973-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01133-06
更新日期:2007-01-01 00:00:00
abstract::Cryptococcal meningoencephalitis develops as a result of hematogenous dissemination of inhaled Cryptococcus neoformans from the lung to the brain. The mechanism(s) by which C. neoformans crosses the blood-brain barrier (BBB) is a key unresolved issue in cryptococcosis. We used both an in vivo mouse model and an in vit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.4985-4995.2004
更新日期:2004-09-01 00:00:00
abstract::Current evidence indicates that Mycobacterium avium infection in patients with AIDS is acquired mostly through the gastrointestinal (GI) tract and that M. avium binds to and invades GI mucosal cells in vitro. Since M. avium is exposed to specific environmental conditions in the GI tract such as changes in pH, low oxyg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3768-3773.1997
更新日期:1997-09-01 00:00:00
abstract::The complement system plays an important role in the host defense against infection, and the formation of the terminal complement complex on the bacterial surface has been shown to be particularly important in killing of gram-negative bacteria. The gram-negative periodontal pathogen Porphyromonas gingivalis is resista...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00304-06
更新日期:2006-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4953-4956.1992
更新日期:1992-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3021-3030.2001
更新日期:2001-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3536-3546.1991
更新日期:1991-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3930-3936.1994
更新日期:1994-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5889-5896.1998
更新日期:1998-12-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-borne rickettsial organism, causes the disease human monocytic ehrlichiosis. The pathogen also causes disease in several other vertebrates, including dogs and deer. In this study, we assessed two clonally purified E. chaffeensis mutants with insertions within the genes Ech_0379 and Ech_06...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00487-15
更新日期:2015-07-01 00:00:00
abstract::Candida albicans is a ubiquitous mucosal commensal that is normally prevented from causing acute or chronic invasive disease. Neutrophils contribute to protection in oral infection but exacerbate vulvovaginal candidiasis. To dissect the role of neutrophils during mucosal candidiasis, we took advantage of a new, transp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00276-17
更新日期:2017-08-18 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6639-6646.2005
更新日期:2005-10-01 00:00:00
abstract::Purified beta-toxin from Clostridium perfringens type C was found to be a single polypeptide chain protein with a molecular weight of approximately 30,000. The toxin was heat labile, with 75% of its activity being inactivated by incubation at 50 degrees C for 5 min. Biological activity of the purified toxin was comple...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.678-680.1978
更新日期:1978-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4648-4654.1996
更新日期:1996-11-01 00:00:00
abstract::Vibrio cholerae causes human infection through ingestion of contaminated food and water, leading to the devastating diarrheal disease cholera. V. cholerae forms matrix-encased aggregates, known as biofilms, in the native aquatic environment. While the formation of V. cholerae biofilms has been well studied, little is ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02617-14
更新日期:2015-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.767-773.1997
更新日期:1997-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.3.1715-1724.2004
更新日期:2004-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00009-07
更新日期:2007-10-01 00:00:00
abstract::Staphylococcus aureus is an important opportunistic pathogen and is the etiological agent of many hospital- and community-acquired infections. The golden pigment, staphyloxanthin, of S. aureus colonies distinguishes it from other staphylococci and related Gram-positive cocci. Staphyloxanthin is the product of a series...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00838-16
更新日期:2017-01-26 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.262-264.1986
更新日期:1986-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00161-08
更新日期:2008-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4485-4488.1993
更新日期:1993-10-01 00:00:00
abstract::Altogether 171 serum specimens from 58 patients with heterophil antibody-positive infectious monomucleosis were studied for antibody response to Epstein-Barr virus (EBV). The sera were tested for fluorescent immunoglobulin G (IgG) and IgM gel-precipitating (GP) and complement-fixing (CF) antibodies to EBV. All 58 pati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.42-51.1975
更新日期:1975-01-01 00:00:00
abstract::Epitope mapping of outer surface protein C (OspC) by using sera from patients with neuroborreliosis led to the identification of one single major immunodominant epitope within the C-terminal 10 amino acid residues. Peptide binding studies and alanine replacement scanning of the C-terminal decapeptide, PVVAESPKKP, reve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Helicobacter pylori is an important human pathogen that causes gastritis and is strongly associated with gastric ulcers, gastric adenocarcinomas, and mucosa-associated lymphoid tissue lymphomas. In response to H. pylori, interleukin-8 (IL-8) is secreted from host cells to attract components of the innate and adaptive ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1523-1531.2005
更新日期:2005-03-01 00:00:00
abstract::A system is described for studying adoptive immunity to tuberculosis in syngeneic mice. Donor mice were immunized with 10(4) BCG intravenously, and lymphoid cells were harvested 28 days later. Adoptive immunity was measured in recipient mice in terms of the inhibition of growth of BCG in the liver and spleen following...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1174-1181.1975
更新日期:1975-06-01 00:00:00