Abstract:
:The bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is an important virulence factor. We examined the role of divergent alleles of bfpA encoding bundlin, the BFP pilin protein, in pilus biogenesis, pilus interactions, and immune responses. We found that the BFP biogenesis machine from an EPEC strain that expresses one bundlin type is capable of assembling all other bundlin types. Furthermore, we found that EPEC strains expressing divergent bundlin types are capable of forming mixed autoaggregates, suggesting that different pilin types can intertwine. However, we found that there was a marked difference between alleles in immunogenicity in both rabbits and mice of a peptide derived from a region of bundlin undergoing apparent diversifying selection. In addition, despite a high degree of cross-reactivity between divergent bundlin proteins, in both mice and rabbits responses appeared to be stronger against the homologous pilin protein than against the heterologous protein. This result was verified using sera from a volunteer study, which demonstrated that the human antibody responses after an initial challenge with live EPEC were stronger against the homologous bundlin protein than against a divergent bundlin protein. However, a repeat challenge induced equivalent responses. These results are consistent with the hypothesis that human immune responses against bundlin exert selective pressure on bfpA sequence divergence.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Fernandes PJ,Guo Q,Donnenberg MSdoi
10.1128/IAI.00009-07subject
Has Abstractpub_date
2007-10-01 00:00:00pages
4687-96issue
10eissn
0019-9567issn
1098-5522pii
IAI.00009-07journal_volume
75pub_type
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doi:10.1128/IAI.01617-14
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journal_title:Infection and immunity
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更新日期:2002-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3930-3936.1994
更新日期:1994-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00364-09
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1739-1744.1995
更新日期:1995-05-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.72.6.3187-3194.2004
更新日期:2004-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00780-09
更新日期:2010-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.00588-19
更新日期:2019-10-18 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00080-06
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2000-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.636-644.1998
更新日期:1998-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2727-2736.1991
更新日期:1991-08-01 00:00:00
abstract::Assembly of adherent biofilms is the key mechanism involved in Staphylococcus epidermidis virulence during device-associated infections. Aside from polysaccharide intercellular adhesin (PIA), the accumulation-associated protein Aap and the extracellular matrix binding protein Embp act as intercellular adhesins, mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01142-10
更新日期:2011-06-01 00:00:00
abstract::B-cell-deficient C57B1/6J mice (suppressed from birth with goat anti-mu) and controls (treated from birth with normal goat serum) were infected with Trypanosoma rhodesiense. There was a significant (P less than 0.01) decrease in duration of survival of the mu-suppressed mice compared with that of controls. Whereas bot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.434-438.1977
更新日期:1977-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.46.2.372-376.1984
更新日期:1984-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1531-1538.1976
更新日期:1976-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.523-528.1990
更新日期:1990-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.92-94.1974
更新日期:1974-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2000-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1663-1667.1989
更新日期:1989-06-01 00:00:00
abstract::Normal ICR mice were infected intravenously, intraperitoneally, or aerogenically with Pasteurella multocida strains isolated from a turkey (S68), calf (V90), or rabbit (J20) lung. Both the turkey and calf isolates were highly virulent for mice and multiplied logarithmically in the lungs, liver, and spleen, resulting i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.779-784.1983
更新日期:1983-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-19
更新日期:2019-08-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2837-2845.2002
更新日期:2002-06-01 00:00:00
abstract::Biological properties of two strains of simian virus 40 (SV40) from brains of two patients with progressive multifocal leukoencephalopathy (PML) have been compared to those of a standard laboratory strain of SV40. Infectivity of both SV40-PML viruses was resistant to treatment with chloroform, low pH, and 50 C for 120...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.173-179.1974
更新日期:1974-07-01 00:00:00
abstract::The P2 porin protein is the major outer membrane protein of nontypeable Haemophilus influenzae and is a potential target of a protective immune response. Nine monoclonal antibodies (MAbs) to P2 were developed by immunizing mice with nontypeable H. influenzae whole organisms. Each MAb reacted exclusively with the homol...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3712-3722.1994
更新日期:1994-09-01 00:00:00
abstract::In this analysis we have examined in detail the effects of low concentrations of zinc on the growth and dimorphism of Candida albicans. Evidence is presented that micromolar concentrations of zinc added to growth cultures grown at 25 degrees C (i) cause a twofold increase in the final concentration of spheres at satio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.348-354.1979
更新日期:1979-10-01 00:00:00