Identification of the Pseudomonas aeruginosa 1244 pilin glycosylation site.


:Previous work (P. Castric, F. J. Cassels, and R. W. Carlson, J. Biol. Chem. 276:26479-26485, 2001) has shown the Pseudomonas aeruginosa 1244 pilin glycan to be covalently bound to a serine residue. N-terminal sequencing of pilin fragments produced from endopeptidase treatment and identified by reaction with a glycan-specific monoclonal antibody indicated that the glycan was present between residue 75 and the pilin carboxy terminus. Further sequencing of these peptides revealed that serine residues 75, 81, 84, 105, 106, and 108 were not modified. Conversion of serine 148, but not serine 118, to alanine by site-directed mutagenesis, resulted in loss of the ability to carry out pilin glycosylation when tested in an in vivo system. These results showed the pilin glycan to be attached to residue 148, the carboxy-terminal amino acid. The carboxy-proximal portion of the pilin disulfide loop, which is adjacent to the pilin glycan, was found to be a major linear B-cell epitope, as determined by peptide epitope mapping analysis. Immunization of mice with pure pili produced antibodies that recognized the pilin glycan. These sera also reacted with P. aeruginosa 1244 lipopolysaccharide as measured by Western blotting and enzyme-linked immunosorbent assay.


Infect Immun


Infection and immunity


Comer JE,Marshall MA,Blanch VJ,Deal CD,Castric P





Has Abstract


2002-06-01 00:00:00












  • A bivalent typhoid live vector vaccine expressing both chromosome- and plasmid-encoded Yersinia pestis antigens fully protects against murine lethal pulmonary plague infection.

    abstract::Live attenuated bacteria hold great promise as multivalent mucosal vaccines against a variety of pathogens. A major challenge of this approach has been the successful delivery of sufficient amounts of vaccine antigens to adequately prime the immune system without overattenuating the live vaccine. Here we used a live a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Galen JE,Wang JY,Carrasco JA,Lloyd SA,Mellado-Sanchez G,Diaz-McNair J,Franco O,Buskirk AD,Nataro JP,Pasetti MF

    更新日期:2015-01-01 00:00:00

  • The psa locus is responsible for thermoinducible binding of Yersinia pseudotuberculosis to cultured cells.

    abstract::Yersinia pseudotuberculosis inv mutant strains cured of the virulence plasmid exhibit thermoinducible adhesion to cultured mammalian cells. To identify the genes responsible for this phenotype, Y. pseudotuberculosis homologs of the Y. enterocolitica ail and the Y. pestis psa loci were identified. Mutations in the Y. p...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yang Y,Merriam JJ,Mueller JP,Isberg RR

    更新日期:1996-07-01 00:00:00

  • Host defense against cholera toxin is strongly CD4+ T cell dependent.

    abstract::This study investigates the role of CD4+ T cells in host defense against cholera enterotoxin-induced diarrhea. Antitoxin immunoglobulin A formation and gut protection against cholera toxin (CT) following oral immunizations with CT were evaluated in normal mice and mice that had been depleted of CD4+ T cells by in vivo...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hörnqvist E,Goldschmidt TJ,Holmdahl R,Lycke N

    更新日期:1991-10-01 00:00:00

  • Monoclonal antibodies against Neisseria gonorrhoeae: production of antibodies directed against a strain-specific cell surface antigen.

    abstract::Hybridomas secreting monoclonal antibodies against an apparent strain-specific cell surface antigen of Neisseria gonorrhoeae were produced. Spleen cells from BALB/c mice immunized with whole gonococci were fused with mouse myeloma cell line Sp2/0, and hybrid cells were selected in culture. One hybridoma that secreted ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nachamkin I,Cannon JG,Mittler RS

    更新日期:1981-05-01 00:00:00

  • Host and tissue specificity of Trichomonas vaginalis is not mediated by its known adhesion proteins.

    abstract::Adhesion of Trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. However, the specific receptors on the host cell have not yet been identified. In this work, the ability of the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Addis MF,Rappelli P,Fiori PL

    更新日期:2000-07-01 00:00:00

  • Effect of oxygen-dependent antimicrobial systems on Legionella pneumophila.

    abstract::Legionella pneumophila was susceptible to the antimicrobial action of oxygen metabolites generated by both the myeloperoxidase-H(2)O(2)-halide and the xanthine oxidase systems. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lochner JE,Friedman RL,Bigley RH,Iglewski BH

    更新日期:1983-01-01 00:00:00

  • Resistance of calves to Cryptosporidium parvum: effects of age and previous exposure.

    abstract::Cryptosporidium parvum is a coccidian parasite that causes diarrheal disease in many vertebrate species, including young (less than or equal to 1 month old) calves. Older calves and adult cattle are resistant to infection. In this study, newborn calves were raised in isolation from C. parvum for 1 week to 3 months bef...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Harp JA,Woodmansee DB,Moon HW

    更新日期:1990-07-01 00:00:00

  • Endotoxin-induced tumor necrosis factor alpha synthesis in murine embryo fibroblasts.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


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    更新日期:1993-05-01 00:00:00

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Simpson CL,Giffard PM,Jacques NA

    更新日期:1995-02-01 00:00:00

  • Characterization of mutations that inactivate the diphtheria toxin repressor gene (dtxR).

    abstract::The diphtheria toxin repressor (DtxR) is an iron-dependent regulator of diphtheria toxin production and iron uptake in Corynebacterium diphtheriae. It is activated in vitro by divalent metal ions including Fe2+, Cd2+, Co2+, Mn2+, Ni2+, and Zn2+. We characterized 20 different mutations in dtxR induced by bisulfite muta...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wang Z,Schmitt MP,Holmes RK

    更新日期:1994-05-01 00:00:00

  • Virulence of Pseudomonas aeruginosa and spontaneous spread of pseudomonas pneumonia in a mink ranch.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shimizu T,Homma JY,Aoyama T,Onodera T,Noda H

    更新日期:1974-07-01 00:00:00

  • Bordetella pertussis risA, but not risS, is required for maximal expression of Bvg-repressed genes.

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    pub_type: 杂志文章


    authors: Stenson TH,Allen AG,Al-Meer JA,Maskell D,Peppler MS

    更新日期:2005-09-01 00:00:00

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Soderberg LS,Tewari RP,Solotorovsky M

    更新日期:1976-06-01 00:00:00

  • Serum amyloid P component bound to gram-negative bacteria prevents lipopolysaccharide-mediated classical pathway complement activation.

    abstract::Although serum amyloid P component (SAP) is known to bind many ligands, its biological function is not yet clear. Recently, it was demonstrated that SAP binds to lipopolysaccharide (LPS). In the present study, SAP was shown to bind to gram-negative bacteria expressing short types of LPS or lipo-oligosaccharide (LOS), ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: de Haas CJ,van Leeuwen EM,van Bommel T,Verhoef J,van Kessel KP,van Strijp JA

    更新日期:2000-04-01 00:00:00

  • Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Giannella RA

    更新日期:1976-07-01 00:00:00

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mattsson E,Rollof J,Verhoef J,Van Dijk H,Fleer A

    更新日期:1994-09-01 00:00:00

  • Molecular characterization of glycoprotein antigens on surface of Treponema pallidum: comparison with nonpathogenic Treponema phagedenis biotype Reiter.

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    pub_type: 杂志文章


    authors: Moskophidis M,Müller F

    更新日期:1984-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Adamou JE,Heinrichs JH,Erwin AL,Walsh W,Gayle T,Dormitzer M,Dagan R,Brewah YA,Barren P,Lathigra R,Langermann S,Koenig S,Johnson S

    更新日期:2001-02-01 00:00:00

  • Effect of antimacrophage serum on dermal tuberculin sensitivity and allergic pulmonary granuloma formation in rabbits.

    abstract::Anti-rabbit alveolar macrophage goat serum (AMS), adsorbed of hemolysins and hemagglutinins, exhibited complement-dependent cytotoxicity for the following rabbit target cells: alveolar macrophages, thymocytes, and kidney (RK-13) cells. The intravenous injection of AMS, sufficient to reduce markedly the dermal tubercul...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Moore VL,Myrvik QN

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  • Opsonic activity of cerebrospinal fluid in experimental cryptococcal meningitis.

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    pub_type: 杂志文章


    authors: Hobbs MM,Perfect JR,Granger DL,Durack DT

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  • Loss of allergen 1 confers a hypervirulent phenotype that resembles mucoid switch variants of Cryptococcus neoformans.

    abstract::Microbial survival in a host is usually dependent on the ability of a pathogen to undergo changes that promote escape from host defense mechanisms. The human-pathogenic fungus Cryptococcus neoformans undergoes phenotypic switching in vivo that promotes persistence in tissue. By microarray and real-time PCR analyses, t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jain N,Li L,Hsueh YP,Guerrero A,Heitman J,Goldman DL,Fries BC

    更新日期:2009-01-01 00:00:00

  • The Yersiniabactin-Associated ATP Binding Cassette Proteins YbtP and YbtQ Enhance Escherichia coli Fitness during High-Titer Cystitis.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Koh EI,Hung CS,Henderson JP

    更新日期:2016-04-22 00:00:00

  • Impaired control of Brucella melitensis infection in Rag1-deficient mice.

    abstract::After intranasal inoculation, Brucella melitensis chronically infects the mononuclear phagocyte system in BALB/c mice, but it causes no apparent illness. Adaptive immunity, which can be transferred by either T cells or antibody from immune to naive animals, confers resistance to challenge infection. The role of innate...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Izadjoo MJ,Polotsky Y,Mense MG,Bhattacharjee AK,Paranavitana CM,Hadfield TL,Hoover DL

    更新日期:2000-09-01 00:00:00

  • Altered Toll-like receptor 9 signaling in Mycobacterium avium subsp. paratuberculosis-infected bovine monocytes reveals potential therapeutic targets.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Arsenault RJ,Li Y,Maattanen P,Scruten E,Doig K,Potter A,Griebel P,Kusalik A,Napper S

    更新日期:2013-01-01 00:00:00

  • Impaired bone resorption by lipopolysaccharide in vivo in mice deficient in the prostaglandin E receptor EP4 subtype.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sakuma Y,Tanaka K,Suda M,Komatsu Y,Yasoda A,Miura M,Ozasa A,Narumiya S,Sugimoto Y,Ichikawa A,Ushikubi F,Nakao K

    更新日期:2000-12-01 00:00:00

  • Influence of cadmium on the phagocytic and microbicidal activity of murine peritoneal macrophages, pulmonary alveolar macrophages, and polymorphonuclear neutrophils.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Loose LD,Silkworth JB,Simpson DW

    更新日期:1978-11-01 00:00:00

  • Susceptibility to reinfection after a primary chlamydial genital infection is associated with a decrease of antigen-specific T cells in the genital tract.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Igietseme JU,Rank RG

    更新日期:1991-04-01 00:00:00

  • In vitro sensitivity of oral, gram-negative, facultative bacteria to the bactericidal activity of human neutrophil defensins.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Miyasaki KT,Bodeau AL,Ganz T,Selsted ME,Lehrer RI

    更新日期:1990-12-01 00:00:00

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ladefoged SA,Birkelund S,Hauge S,Brock B,Jensen LT,Christiansen G

    更新日期:1995-01-01 00:00:00

  • Candida albicans White-Opaque Switching Influences Virulence but Not Mating during Oropharyngeal Candidiasis.

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    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Solis NV,Park YN,Swidergall M,Daniels KJ,Filler SG,Soll DR

    更新日期:2018-05-22 00:00:00