Abstract:
:Purified and concentrated Venezuelan equine encephalitis (VEE) virus derived from tissue cultures, rendered noninfectious by ionizing radiation with retention of in vitro serological activity, also retained a high level of immunogenicity. In mice, fluid vaccines afforded excellent protection against lethal challenge with homologous Trinidad strain VEE virus. A direct relationship was observed between concentration of vaccine or number of injections and survival. One intraperitoneal inoculation of undiluted vaccine protected essentially all mice challenged 21 days later with 100,000 mouse intraperitoneal LD(50) of virus. Similarly, mice receiving three injections of vaccines diluted 1:100 were completely protected. Noninfectious VEE virus preparations combined with adjuvant 65, a nontoxic metabolizable vehicle, were likewise very effective in protecting mice immunized intraperitoneally or subcutaneously against lethal challenge. Guinea pigs immunized subcutaneously with adjuvant-combined vaccine survived lethal challenge of 1,000,000 guinea pig intraperitoneal LD(50).
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Gruber Jdoi
10.1128/IAI.3.4.574-579.1971keywords:
subject
Has Abstractpub_date
1971-04-01 00:00:00pages
574-9issue
4eissn
0019-9567issn
1098-5522journal_volume
3pub_type
杂志文章abstract::Rhodococcus equi is a facultative intracellular pathogen of macrophages, relying on the presence of a conjugative virulence plasmid harboring a 21-kb pathogenicity island (PAI) for growth in host macrophages. The PAI encodes a family of 6 virulence-associated proteins (Vaps) in addition to 20 other proteins. The contr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00230-15
更新日期:2015-08-01 00:00:00
abstract::Previous examination of thin sections of L929 cells heavily infected with the Q fever Priscilla isolate by conventional transmission electron microscopy indicated that the rickettsiae resided within multiple vacuoles. The present study using high-voltage electron microscopy and three-dimensional reconstruction reveale...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4485-4488.1993
更新日期:1993-10-01 00:00:00
abstract::The Lyme disease spirochete Borrelia burgdorferi reduces the expression of outer surface protein C (OspC) in response to the development of an anti-OspC humoral response, leading to the hypothesis that the ability to repress OspC expression is critical for the pathogen to proceed to chronic infection. B. burgdorferi w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00713-06
更新日期:2006-09-01 00:00:00
abstract::Fibrosis is a significant complication of intestinal disorders associated with microbial dysbiosis and pathobiont expansion, notably Crohn's disease (CD). Mechanisms that favor fibrosis are not well understood, and therapeutic strategies are limited. Here we demonstrate that colitis-susceptible Il10-deficient mice dev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00587-19
更新日期:2019-10-18 00:00:00
abstract::Human plasma was bactericidal for small numbers of Klebsiella pneumoniae, but larger numbers grew slowly over a period of 24 h. Human polymorphs in a clot of autologous plasma had a bacteriostatic effect on relatively large numbers of bacteria for up to 24 h and were much more effective than plasma alone. The bacteric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.723-730.1986
更新日期:1986-03-01 00:00:00
abstract::We have developed experimental murine Campylobacter infection models which demonstrate efficient establishment and reproducible, high-level colonization. Following oral inoculation, wild-type C3H mice with normal enteric flora were colonized inconsistently and inefficiently by C. jejuni strain 81-176. However, C3H mic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01094-05
更新日期:2006-09-01 00:00:00
abstract::Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05851-11
更新日期:2012-02-01 00:00:00
abstract::In this study we tested the in vitro and in vivo anti-Cryptococcus neoformans activity of an antilaminarin (anti-beta-glucan) monoclonal antibody (MAb 2G8) (immunoglobulin G2b) which was previously shown to inhibit the growth of beta-glucan-exposing Candida albicans cells. Here we show that MAb 2G8 binds to the cell w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00278-07
更新日期:2007-11-01 00:00:00
abstract::Previous studies in our laboratory have indicated that naturally resistant, inbred DBA/2J mice mount a greater serum antibody response to Pseudomonas aeruginosa 19660 than susceptible C57BL/6J mice. However, the specificity of the antibody produced was not known. The present study examines the specificity and kinetics...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1984-1990.1991
更新日期:1991-06-01 00:00:00
abstract::Escherichia coli strains expressing the K1 capsule are a major cause of sepsis and meningitis in human neonates. The development of these diseases is dependent on the expression of a range of virulence factors, many of which remain uncharacterized. Here, we show that all but 1 of 34 E. coli K1 neonatal isolates carrie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00716-15
更新日期:2015-09-01 00:00:00
abstract::Analysis of the specificity of bactericidal antibodies in normal, convalescent, and postvaccination human sera is important in understanding human immunity to meningococcal infections and can aid in the design of an effective group B vaccine. A collection of human sera, including group C and group B convalescent-phase...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05125-11
更新日期:2011-10-01 00:00:00
abstract::Streptococcus suis has received increasing attention for its involvement in severe human infections worldwide as well as in multidrug resistance. Two-component signaling systems (TCSSs) play important roles in bacterial adaptation to various environmental stimuli. In this study, we identified a novel TCSS located in S...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00096-18
更新日期:2018-06-21 00:00:00
abstract::A major goal of current vaccine development is the induction of strong immune responses against protective antigens delivered by mucosal routes. One of the most promising approaches in that respect relies on the use of live recombinant vaccine carriers. In this study, Mycobacterium bovis BCG was engineered to produce ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5669-5676.1998
更新日期:1998-12-01 00:00:00
abstract::Mice were infected with Chlamydia trachomatis (L2/434) and at various intervals after infection their lymphoid cells were tested directly for cytotoxic activity against Chlamydia-infected L929 cells. In a modified cell-mediated lympholysis assay, splenic, lymph node, and peritoneal exudate cells did not significantly ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1271-1274.1983
更新日期:1983-03-01 00:00:00
abstract::Acute lethality was induced in rabbits by the sequential injection of Propionibacterium acnes and lipopolysaccharide (LPS). P. acnes induced the infiltration of inflammatory cells into the liver lobules during the early phase, and LPS in the late phase caused death in association with pathological changes mimicking he...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4812-4817.1995
更新日期:1995-12-01 00:00:00
abstract::The complement system plays an important role in the host defense against infection, and the formation of the terminal complement complex on the bacterial surface has been shown to be particularly important in killing of gram-negative bacteria. The gram-negative periodontal pathogen Porphyromonas gingivalis is resista...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00304-06
更新日期:2006-09-01 00:00:00
abstract::Inactivated Marek's disease virus-infected chicken kidney cells and inactivated MSB-1 lymphoblastoid Marek's disease tumor cells were used to immunize chickens as virus- and tumor-associated antigens, respectively. Immune and nonimmune birds were then challenged by exposure to live virulent Marek's disease virus. Both...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.547-553.1979
更新日期:1979-11-01 00:00:00
abstract::Athymic BALB/c nude mice and euthymic BALB/c mice were infected with Rickettsia akari by the intraperitoneal route. The rickettsialpox infection was terminated in euthymic mice with only two intraperitoneal injections of the antibiotic oxytetracycline, whereas prolonged treatment was necessary to terminate the infecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::The serum opacity factor (SOF) of Streptococcus pyogenes is a serotyping tool and pathogenesis factor. Using SOF-coated latex beads in cell adherence assays and antiserum directed against SOF in S. pyogenes-HEp-2 cell adherence inhibition experiments, we demonstrate SOF involvement in the fibronectin-mediated adherenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4302-4308.2004
更新日期:2004-07-01 00:00:00
abstract::Hydrosalpinx induction in mice by Chlamydia muridarum infection, a model that has been used to study C. trachomatis pathogenesis in women, is known to depend on the cryptic plasmid that encodes eight genes designated pgp1 to pgp8. To identify the plasmid-encoded pathogenic determinants, we evaluated C. muridarum trans...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02576-14
更新日期:2014-12-01 00:00:00
abstract::Monoclonal antibody MASC1-MR9 of isotype immunoglobulin M was generated in LOU/C rats by immunization with heat-killed Salmonella thompson (serogroup C1, O-antigen 6,7 lipopolysaccharide) bacteria which had been further enriched for O-antigen by coating with homologous lipopolysaccharide. Eight monoclonal antibodies w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.229-233.1991
更新日期:1991-01-01 00:00:00
abstract::In gram-negative sepsis, the activation of polymorphonuclear leukocytes (PMN) by lipopolysaccharide (LPS) and the resulting production of superoxide and other oxygen radicals may be an important cause of tissue damage. A suppression of the PMN response to LPS stimulation would be therapeutically beneficial. The aim of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4320-4324.1994
更新日期:1994-10-01 00:00:00
abstract::The polysaccharide antigen preparations from serotype a and serotype d strains of Streptococcus mutans contained both a serotype-specific antigenic determinant and a common a-d antigenic determinant, as demonstrated by agar gel diffusion studies and a quantitative cross-precipitin assay. The chromatographically purifi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.791-798.1975
更新日期:1975-10-01 00:00:00
abstract::A complex of trehalose-6, 6'-dimycolate (cord factor) and methylated bovine serum albumin was prepared, and its effect on the toxicity of cord factor in mice was studied. Either the active immunization with cord factor-methylated bovine serum albumin complex or the passive transfer of sera of rabbits vaccinated with t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.2.203-212.1972
更新日期:1972-02-01 00:00:00
abstract::Numerous studies have reported that asialo-GM(1), gangliotetraosylceramide, or moieties serve as epithelial cell receptors for Pseudomonas aeruginosa. Usually this interaction is confirmed with antibodies to asialo-GM(1). However, few, if any, of these reports have evaluated the binding of fresh clinical isolates of P...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.719-729.2001
更新日期:2001-02-01 00:00:00
abstract::An exoprotein of Staphylococcus aureus 100 that elicited a positive ileal loop response in the rabbit model was investigated in this study. The protein, as it occurred in the culture supernatant fluid, could be detected initially in the late log phase of growth under aerobic and anaerobic conditions. It was stable und...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.298-303.1977
更新日期:1977-11-01 00:00:00
abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal pneumonia, sepsis, and meningitis. An in silico genome analysis indicated that GBS strain NEM316 encodes 35 proteins containing an LPXTG motif which are thought to be covalently linked to the peptidoglycan by an enzyme called sorta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3342-3350.2005
更新日期:2005-06-01 00:00:00
abstract::Cytomegalovirus (CMV) infection is common after renal transplantation, and cytomegaloviruria occurs in about two-thirds of the recipients. These observations suggest that the allografts may be a site of latent infection with CMV and its reactivation may be the source of viruria. To investigate this possibility, 130 ki...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.699-703.1978
更新日期:1978-02-01 00:00:00
abstract::Listeria monocytogenes colonized the gastrointestinal tract of adult germfree rats (10(10) to 10(11)/g, dry weight) within 24 h after oral exposure. Between 3 and 14 days after monoassociation, L. monocytogenes caused a self-limiting pseudomembranous colitis, bacteremia, and infection of the spleen and liver. Monoasso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.323-331.1981
更新日期:1981-04-01 00:00:00
abstract::We report the cloning of the gene encoding the 32-kDa lipoprotein, designated LipL32, the most prominent protein in the leptospiral protein profile. We obtained the N-terminal amino acid sequence of a staphylococcal V8 proteolytic-digest fragment to design an oligonucleotide probe. A Lambda-Zap II library containing E...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2276-2285.2000
更新日期:2000-04-01 00:00:00