Abstract:
:The Re mutant of Salmonella minnesota adheres in much greater numbers than the wild type to endothelial cells derived from the bovine pulmonary artery. Since the Re mutant is distinguished from wild-type S. minnesota by its ability to bind C1q and since endothelial cells possess receptors for C1q, we examined the role of C1q in the phagocytosis of the S. minnesota Re mutant. First, preincubating endothelial cells with C1q-enriched medium resulted in increased adherence of the Re mutant (17.9 x 10(4) versus 6.6 x 10(4]. Second, preincubating the Re mutant with C1q-enriched medium resulted in increased numbers of adherent bacteria (62.1 x 10(4) versus 6.6 x 10(4]. Preincubation of both endothelial cells and bacteria with C1q-enriched medium resulted in increased adherence above control levels but less adherence than when either cells or bacteria were preincubated separately in C1q-enriched medium. If serum depleted of C1q was used for preincubation of endothelial cells or bacteria, adherence was reduced below control levels. Thus, C1q plays an important role in the initial steps (recognition, binding, and ingestion) of phagocytosis. Next, the role of C1q was investigated in the respiratory burst response. Levels of superoxide anion released from endothelial cells 15 min after phagocytosis of the Re mutant (100 bacteria per endothelial cell) were assayed by measurement of the superoxide dismutase-inhibitable reduction of ferricytochrome c. Superoxide anion release was increased during phagocytosis of the Re mutant (35 nmol of O2- per 3 x 10(6) endothelial cells) and was also elevated above control values by incubation with soluble C1q (10 nmol of O2- per 3 x 10(6) endothelial cells). These results indicate a role for C1q in both the ingestion and the response of endothelial cells to the S. minnesota Re mutant.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Ryan US,Schultz DR,Goodwin JD,Vann JM,Selvaraj MP,Hart MAdoi
10.1128/IAI.57.5.1356-1362.1989subject
Has Abstractpub_date
1989-05-01 00:00:00pages
1356-62issue
5eissn
0019-9567issn
1098-5522journal_volume
57pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.40.1.265-272.1983
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doi:10.1128/IAI.63.10.3994-4002.1995
更新日期:1995-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.57.5.1409-1412.1989
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