Abstract:
:Several studies have demonstrated roles for eosinophils during innate and adaptive immune responses to helminth infections. However, evidence that eosinophils are capable of initiating an immune response to parasite antigens is lacking. The goal of the present in vitro study was to investigate the potential of eosinophils to serve as antigen-presenting cells (APC) and initiate an immune response to parasite antigens. Purified eosinophils were exposed to soluble Strongyloides stercoralis antigens, and the expression of various surface markers involved in cell activation was examined. Antigen-exposed eosinophils showed a sixfold increase in expression levels of CD69 and major histocompatibility complex (MHC) class II, a fourfold increase in levels of T-cell costimulatory molecule CD86, and a twofold decrease in levels of CD62L compared to eosinophils cultured in medium containing granulocyte-macrophage colony-stimulating factor. The ability of eosinophils to present antigen to T cells was determined by culturing them with T cells in vitro. Eosinophils pulsed with antigen stimulated antigen-specific primed T cells and CD4+ T cells to increase interleukin-5 (IL-5) production. The blocking of MHC class II expression on eosinophils inhibited their ability to induce IL-5 production by CD4+ T cells in culture. Antigen-pulsed eosinophils were able to prime naïve T cells and CD4+ T cells in culture and polarized them into Th2 cells producing IL-5 similar to that induced by antigen-loaded dendritic cells. These results demonstrate that eosinophils are capable of activating antigen-specific Th2 cells inducing the release of cytokines and assist in the priming of naïve T cells to initiate Th2 responses against infection. This study highlights the potential of eosinophils to actively induce immune responses against infection by amplifying antigen-specific Th2-cell responses.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Padigel UM,Lee JJ,Nolan TJ,Schad GA,Abraham Ddoi
10.1128/IAI.02067-05subject
Has Abstractpub_date
2006-06-01 00:00:00pages
3232-8issue
6eissn
0019-9567issn
1098-5522pii
74/6/3232journal_volume
74pub_type
杂志文章abstract::The lack of specific T-cell help in immune responses to thymus-independent antigens results in weak, predominantly immunoglobulin M-mediated immunity with little or no memory. In the work presented here we show how the exogenous stimulation of CD40 by monoclonal antibodies can mimic T-cell help, resulting in enhanced ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3637-3640.1999
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journal_title:Infection and immunity
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doi:10.1128/IAI.63.2.381-388.1995
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1760-1765.1988
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.669-673.1973
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.3116-3121.1990
更新日期:1990-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.3.544-549.1975
更新日期:1975-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.876-886.2006
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2276-2285.2000
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4146-4151.1997
更新日期:1997-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2800-2805.1994
更新日期:1994-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.159-165.1992
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.721-728.1978
更新日期:1978-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2767-2773.1991
更新日期:1991-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.1.124-129.1984
更新日期:1984-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.253-259.1979
更新日期:1979-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3122-3128.1994
更新日期:1994-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.756-761.1988
更新日期:1988-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2535-2541.1990
更新日期:1990-08-01 00:00:00
abstract::Bacterial adherence to mucosal surfaces is an important virulence trait of pathogenic bacteria. Adhesion of enterotoxigenic Escherichia coli (ETEC) to the intestine is mediated by a number of antigenically distinct colonization factors (CFs). One of the most common CFs is CFA/I. This has a fimbrial structure composed ...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00