Abstract:
:Neutrophil collagenase (matrix metalloproteinase 8 [MMP-8]) is an important mediator of tissue destruction in inflammatory diseases. Studies of anaerobic periodontal infections have shown that active MMP-8 in gingival crevicular fluid is associated with the degradation of periodontal tissues in progressive periodontitis whereas the latent enzyme is predominant in gingivitis. Since the activation of MMP-8 appears to be a crucial step in periodontitis, we have examined the activation of MMP-8 in gingival crevicular fluid samples by using a soluble biotinylated collagen substrate. Analysis of gingival crevicular fluid in periodontitis, gingivitis, and controls revealed sixfold (P < 0.001)-higher levels of active collagenase in periodontitis (n = 12) samples compared to gingivitis (n = 17) samples, which exhibited low levels of activity, while controls (n = 25) showed no activity. After gingival crevicular fluid was collected, no further activation of latent collagenase occurred in vitro. Although both MMP-1 and MMP-8, but not MMP-13, could be detected by immunoblots, blocking antibodies to MMP-1 showed that collagenase activity was largely contributed by MMP-8, which was localized to the matrix of diseased tissues. The MMP-8 in gingival crevicular fluid migrated primarily as a 60-kDa form with smaller amounts of a 78-kDa species, whereas MMP-8 isolated from peripheral neutrophils migrated at 70 and 89 kDa, corresponding to active and latent forms of the enzyme, respectively. Most of the MMP-8 in the 60- and 70-kDa bands selectively bound to tissue inhibitor of metalloproteinase 2 and collagen, indicating that most, but not all, of the enzyme in these bands was in an activated form. However, the amounts of the 78- and 60-kDa forms from gingival crevicular fluid in different samples did not correlate (r2 = 0.028) with the latent and active enzyme measured by collagenase assay. Collectively, these studies have identified distinct forms of latent and active MMP-8 in gingival crevicular fluid that appear to result from a unique activation mechanism that occurs in periodontitis. The complexity of MMP-8 activation is further indicated by the presence of latent, activated, and superactivated forms of MMP-8 in the 60- and 70-kDa bands obtained from gingival crevicular fluid and neutrophil samples, respectively.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Romanelli R,Mancini S,Laschinger C,Overall CM,Sodek J,McCulloch CAdoi
10.1128/IAI.67.5.2319-2326.1999keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
2319-26issue
5eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::Oral treponemes are considered to be important in the development and progression of periodontal diseases. We investigated the mechanisms of recognition and activation of human gingival epithelial cells (HGEC) with the oral treponemes Treponema denticola, Treponema vincentii, and Treponema medium and their outer membr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.717-725.2003
更新日期:2003-02-01 00:00:00
abstract::Newly recognized Fic family virulence proteins may be important in many bacterial pathogens. To relate cellular mechanisms to pathogenesis and immune protection, we studied the cytotoxicity of the Histophilus somni immunoglobulin-binding protein A (IbpA) direct repeat 2 Fic domain (DR2/Fic) for natural host target cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01277-09
更新日期:2010-05-01 00:00:00
abstract::In the present study, the immunodepressive effects of canine distemper virus (CDV) infection of dogs on two parameters of lymphocyte function, namely phytomitogen-induced cellular proliferation and skin allograft rejection, were investigated. Infection of susceptible gnotobiotic dogs with virulent R252-CDV resulted in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1069-1078.1975
更新日期:1975-05-01 00:00:00
abstract::The adenylate cyclase-hemolysin of Bordetella pertussis consists of a cell-invasive N-terminal adenylate cyclase domain linked to a C-terminal RTX hemolysin containing extensive glycine-rich repeats. The toxin is an essential virulence factor required in the initial stages of infection. Adenylate cyclase-hemolysin was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3309-3315.1995
更新日期:1995-09-01 00:00:00
abstract::Mannheimia haemolytica, a commensal organism of the upper respiratory tract in cattle, is the principal bacterial pathogen associated with the bovine respiratory disease complex. Adherence to the respiratory mucosa is a crucial event in its pathogenesis. However, the bacterial components that contribute to this proces...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00312-08
更新日期:2009-01-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major etiologic agent of nontyphoid salmonellosis in the United States. S. Enteritidis persistently and silently colonizes the intestinal and reproductive tract of laying hens, resulting in contaminated poultry products. The consumption of contaminated poul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02387-14
更新日期:2014-12-01 00:00:00
abstract::Deoxyribonucleic acid (DNA) base composition, as measured by guanine plus cytosine (G + C) content, was determined in 17 strains of Sporothrix schenckii and Ceratocystis species. The average G + C content for S. schenckii was 54.7 +/- 0.2 mol%. No strain of Ceratocystis, with the exception of C. minor, gave values for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.5.934-938.1974
更新日期:1974-05-01 00:00:00
abstract::Knowledge of the in vivo physiology and metabolism of Streptococcus pneumoniae is limited, even though pneumococci rely on efficient acquisition and metabolism of the host nutrients for growth and survival. Because the nutrient-limited, hypoxic host tissues favor mixed-acid fermentation, we studied the role of the pne...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00178-09
更新日期:2009-12-01 00:00:00
abstract::The pili of Serratia marcescens US5 isolated from a patient with urinary tract infection were purified and characterized. During the aeration culture, the pili were detached from the bacteria and were precipitated by the addition of ammonium sulfate. The purification of the pili was carried out by ion-exchange chromat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.295-300.1984
更新日期:1984-11-01 00:00:00
abstract::Expression of virulence determinants by Bordetella pertussis, the primary etiological agent of whooping cough, is regulated by the BvgAS two-component regulatory system. The role of a second two-component regulatory system, encoded by risAS, in this process is not defined. Here, we show that mutation of B. pertussis r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5995-6004.2005
更新日期:2005-09-01 00:00:00
abstract::Clostridium difficile is a spore-forming bacterium that can reside in animals and humans. C. difficile infection causes a variety of clinical symptoms, ranging from diarrhea to fulminant colitis. Disease is mediated by TcdA and TcdB, two large enterotoxins released by C. difficile during colonization of the gut. In th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01341-12
更新日期:2013-08-01 00:00:00
abstract::Bacillus anthracis, the etiological agent of anthrax disease, is a proven weapon of bioterrorism. Currently, the only licensed vaccine against anthrax in the United States is AVA Biothrax, which, although efficacious, suffers from several limitations. This vaccine requires six injectable doses over 18 months to stimul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00828-08
更新日期:2009-04-01 00:00:00
abstract::Previous reports have demonstrated that heat-stable (A-type) capsule on piliated enterotoxigenic Escherichia coli enhances colonization of enterotoxigenic E. coli in the small intestine and enhances virulence of enterotoxigenic E. coli. In this report, four encapsulated enterotoxigenic E. coli strains and one encapsul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.737-740.1984
更新日期:1984-09-01 00:00:00
abstract::Streptococcus pneumoniae (the pneumococcus), a leading cause of bacterial disease, is most commonly carried in the human nasopharynx. Colonization induces inflammation that promotes the organism's growth and transmission. This inflammatory response is dependent on intracellular sensing of bacterial components that acc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-15
更新日期:2015-08-01 00:00:00
abstract::The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.1.91-97.1972
更新日期:1972-01-01 00:00:00
abstract::Protective immunity against Toxoplasma gondii is known to be mediated mainly by T lymphocytes and gamma interferon (IFN-gamma). The contribution of CD4(+) and CD8(+) T-lymphocyte subsets to protective immune responses against T. gondii infection, triggered by a GRA1 (p24) DNA vaccine, was assessed in this study. In vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.309-316.2003
更新日期:2003-01-01 00:00:00
abstract::We investigated a possible cause-and-effect relationship between sensitization against Actinomyces viscosus Nyl and destructive periodontal disease in RIC-Sprague-Dawley rats. Germfree rats (66) were either immunized with A. viscosus Nyl (day 20) or orally infected with A. viscosus Nyl (days 38 and 39) or both. We mea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.971-977.1981
更新日期:1981-03-01 00:00:00
abstract::Minicells are small, anucleate cells resulting from aberrant cell divisions at the polar ends of bacilli. We have isolated minicell-producing mutant strains of Shigella flexneri 2a (MC-I) and Shigella dysenteriae 1 (MC-V) after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Microscopically, broth cultures of M...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.297-302.1980
更新日期:1980-10-01 00:00:00
abstract::Some species of the genus Mycoplasma code for the arginine deiminase pathway (ADI), which enables these bacteria to produce ATP from arginine by the successive reaction of three enzymes: arginine deiminase (ArcA), ornithine carbamoyltransferase (ArcB), and carbamate kinase (ArcC). It so far appears that independently ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00441-13
更新日期:2013-10-01 00:00:00
abstract::Escherichia coli strains expressing the K1 capsule are a major cause of sepsis and meningitis in human neonates. The development of these diseases is dependent on the expression of a range of virulence factors, many of which remain uncharacterized. Here, we show that all but 1 of 34 E. coli K1 neonatal isolates carrie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00716-15
更新日期:2015-09-01 00:00:00
abstract::The type III capsular polysaccharide of group B streptococci (GBS) consists of a linear backbone with short side chains ending in residues of N-acetylneuraminic acid, or sialic acid. The presence of sialic acid on the surface of the organism inhibits activation of the alternative pathway of complement and is thought t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.392-400.1992
更新日期:1992-02-01 00:00:00
abstract::A simple and convenient method was developed for the preparation of Streptococcus pneumoniae type 14 polysaccharide (Pn14PS)-tetanus toxoid (TT) conjugate vaccines, using terminally linked Pn14PS fragments of different lengths. Native Pn14PS was simultaneously depolymerized and activated for conjugation by partial N-d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2441-2446.1998
更新日期:1998-06-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections. These bacteria undertake a multistage infection cycle involving invasion of and proliferation within urinary tract epithelial cells, leading to the rupture of the host cell and dispersal of the bacteria, some of which have a highly...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00884-19
更新日期:2020-08-19 00:00:00
abstract::The data presented indicate that in experimental Pseudomonas aeruginosa infection of mice, protease enhances the virulence of the organism. Anesthetized CBA/Lü mice were subjected to a 15-s flame burn and infected with a wild-type protease-producing strain and two of its protease-deficient mutants. The average bacteri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.181-187.1979
更新日期:1979-04-01 00:00:00
abstract::Pseudomonas aeruginosa is a gram-negative bacillus that is a major cause of morbidity and mortality in immunosuppressed patients, burn patients, and patients with cystic fibrosis. Although immunity to these bacteria has been associated with serum antibody, more recent evidence suggests that T-cell-mediated immunity ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.670-674.1983
更新日期:1983-05-01 00:00:00
abstract::MtsABC is a Streptococcus pyogenes ABC transporter which was previously shown to be involved in iron and zinc accumulation. In this study, we showed that an mtsABC mutant has impaired growth, particularly in a metal-depleted medium and an aerobic environment. In metal-depleted medium, growth was restored by the additi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2656-2664.2003
更新日期:2003-05-01 00:00:00
abstract::Bacteria have evolved strategies to resist killing by antimicrobial peptides (APs), important effectors of innate immunity. The sap (sensitivity to antimicrobial peptides) operon confers resistance to AP-mediated killing of Salmonella. We have recently shown that sapA gene expression is upregulated in the middle ear i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.599-608.2005
更新日期:2005-01-01 00:00:00
abstract::We previously reported that infection by Mycobacterium tuberculosis, the causative agent of tuberculosis, leads to secretion of alpha/beta interferon (IFN-alpha/beta). While IFN-alpha/beta ordinarily stimulates formation of signal transducer and stimulator of transcription-1 (STAT-1) homodimers and IFN-stimulated gene...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2487-2497.2003
更新日期:2003-05-01 00:00:00
abstract::The affinity of uropathogenic Escherichia coli to kidneys and bladders of experimentally infected mice was shown to be determined in part by the adhesive properties of the infecting bacteria. Mice were infected with various pairwise combinations of two homogeneic sets of bacteria: (i) mutants derived from a human pyel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.265-272.1983
更新日期:1983-04-01 00:00:00
abstract::Brazilian purpuric fever (BPF)-associated Haemophilus influenzae biogroup aegyptius strain F3031 contains two identical copies of a five gene cluster (hifA to hifE) encoding pili similar to well-characterized Hif fimbriae of H. influenzae type b. HifE, the putative pilus tip adhesin of F3031, shares only 40% amino aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1622-1631.1998
更新日期:1998-04-01 00:00:00