Abstract:
:Expression of virulence determinants by Bordetella pertussis, the primary etiological agent of whooping cough, is regulated by the BvgAS two-component regulatory system. The role of a second two-component regulatory system, encoded by risAS, in this process is not defined. Here, we show that mutation of B. pertussis risA does not affect Bvg-activated genes or proteins. However, mutation of risA resulted in greatly diminished expression of Bvg-repressed antigens and decreased transcription of Bvg-repressed genes. In contrast, mutation of risS had no effect on the expression of Bvg-regulated molecules. Mutation of risA also resulted in decreased bacterial invasion in a HeLa cell model. However, decreased invasion could not be attributed to the decreased expression of Bvg-repressed products, suggesting that mutation of risA may affect the expression of a variety of genes. Unlike the risAS operons in B. parapertussis and B. bronchiseptica, B. pertussis risS is a pseudogene that encodes a truncated RisS sensor. Deletion of the intact part of the B. pertussis risS gene does not affect the expression of risA-dependent, Bvg-repressed genes. These observations suggest that RisA activation occurs through cross-regulation by a heterologous system.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Stenson TH,Allen AG,Al-Meer JA,Maskell D,Peppler MSdoi
10.1128/IAI.73.9.5995-6004.2005keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
5995-6004issue
9eissn
0019-9567issn
1098-5522pii
73/9/5995journal_volume
73pub_type
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journal_title:Infection and immunity
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