Abstract:
:Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major etiologic agent of nontyphoid salmonellosis in the United States. S. Enteritidis persistently and silently colonizes the intestinal and reproductive tract of laying hens, resulting in contaminated poultry products. The consumption of contaminated poultry products has been identified as a significant risk factor for human salmonellosis. To understand the mechanisms S. Enteritidis utilizes to colonize and persist in laying hens, we used selective capture of transcribed sequences to identify genes overexpressed in the HD11 chicken macrophage cell line and in primary chicken oviduct epithelial cells. From the 15 genes found to be overexpressed in both cell types, we characterized the antimicrobial peptide resistance (AMPR) genes, virK and ybjX, in vitro and in vivo. In vitro, AMPR genes were required for natural morphology, motility, secretion, defense against detergents such as EDTA and bile salts, and resistance to antimicrobial peptides polymyxin B and avian β-defensins. From this, we inferred the AMPR genes play a role in outer membrane stability and/or modulation. In the intestinal tract, AMPR genes were involved in early intestinal colonization and fecal shedding. In the reproductive tract, virK was required in early colonization whereas a deletion of ybjX caused prolonged ovary colonization and egg deposition. Data from the present study indicate that AMPR genes are differentially utilized in various host environments, which may ultimately assist S. Enteritidis in persistent and silent colonization of chickens.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
McKelvey JA,Yang M,Jiang Y,Zhang Sdoi
10.1128/IAI.02387-14subject
Has Abstractpub_date
2014-12-01 00:00:00pages
5185-202issue
12eissn
0019-9567issn
1098-5522pii
IAI.02387-14journal_volume
82pub_type
杂志文章abstract::Mannheimia haemolytica, a commensal organism of the upper respiratory tract in cattle, is the principal bacterial pathogen associated with the bovine respiratory disease complex. Adherence to the respiratory mucosa is a crucial event in its pathogenesis. However, the bacterial components that contribute to this proces...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00312-08
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abstract::The lungs of conventional rats with chronic pneumonia contained Streptobacillus moniliformis and Mycoplasma pulmonis. These organisms singly and in combination failed to produce lung disease when inoculated into specific pathogen-free rats. On the other hand, diseased lung homogenate not containing cultivable organism...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.1.83-91.1972
更新日期:1972-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00775-15
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.3.1715-1724.2004
更新日期:2004-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.6.976-981.1972
更新日期:1972-06-01 00:00:00
abstract::Chitinases of pathogens have been proposed as potential targets of vaccines or specific inhibitors. We studied the genomic organization, transcript levels, developmental expression, and biological function of chitinases in the rodent filarial nematode Acanthocheilonema viteae, a model organism for human-pathogenic fil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00701-07
更新日期:2008-01-01 00:00:00
abstract::Lipid X, a precursor in the biosynthesis of lipid A, has been claimed to possess most of the immunostimulatory activity but none of the toxicity of endotoxin. However, recent work shows that synthetic lipid X can be contaminated with small amounts of N,O-acylated disaccharide-1-phosphate (H. Aschauer, A. Grob, J. Hild...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02810-14
更新日期:2015-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.3793-3799.1999
更新日期:1999-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.14-22.1975
更新日期:1975-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.4.1110-1116.1976
更新日期:1976-04-01 00:00:00
abstract::Coagulation and inflammation are interconnected, suggesting that coagulation plays a key role in the inflammatory response to pathogens. A phenome-wide association study (PheWAS) was used to identify clinical phenotypes of patients with a polymorphism in coagulation factor X. Patients with this single nucleotide polym...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00031-19
更新日期:2019-04-23 00:00:00
abstract::Twenty-six Pseudomonas aeruginosa strains from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum, whereas 15 strains were agglutinated with more than one serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.170-177.1983
更新日期:1983-10-01 00:00:00
abstract::The guinea pig migration inhibition technique, an accepted in vitro correlate of delayed hypersensitivity, has been adapted to a murine system. Peritoneal exudate cells from CF-1 mice vaccinated with viable cells of the H37Ra strain of Mycobacterium tuberculosis were inhibited in vitro by purified protein derivative (...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.2.190-195.1973
更新日期:1973-02-01 00:00:00
abstract::Previously, we reported that mutants of Legionella pneumophila lacking a type II secretion (T2S) system elicit higher levels of cytokines (e.g., interleukin-6 [IL-6]) following infection of U937 cells, a human macrophage-like cell line. We now show that this effect of T2S is also manifest upon infection of human THP-1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00897-16
更新日期:2017-03-23 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.254-261.1979
更新日期:1979-10-01 00:00:00
abstract::Livers of normal mice trapped over 80% of intravenously injected 51Cr-labeled lipopolysaccharide after 1 h. Liver fractionation studies showed that nearly 45% of the labeled endotoxin was associated with cell nuclei, 20% with the mitochondrial-lysosomal fraction, and approximately 30% with the cell sap. Analysis of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.100-105.1976
更新日期:1976-07-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) initiates infection by colonizing the upper respiratory tract and is a common cause of localized respiratory tract disease. Previous work has established that the NTHi HMW1 and HMW2 proteins are potent adhesins that mediate efficient in vitro adherence to cultured human respir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00153-16
更新日期:2016-09-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Gamma interferon (IFN-gamma), a pleiotropic cytokine, is now known to be produced by macrophages as well as by NK cells, gammadelta cells, and activated T cells. The autocrine biological functions of IFN-gamma on the macrophage include the upregulation of major histocompatibility complex MHC class II and the activatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3605-3610.2001
更新日期:2001-06-01 00:00:00
abstract::Murine hybridoma cell lines were developed which synthesized monoclonal antibodies against Actinobacillus actinomycetemcomitans-associated antigens. Monoclonal antibodies specific for an antigen(s) common to all A. actinomycetemcomitans isolates tested but not detected on other gram-negative oral plaque microorganisms...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1394-1398.1988
更新日期:1988-05-01 00:00:00
abstract::Cathepsin G (CatG) is a serine protease found in the azurophilic granules of monocytes that is known to have antimicrobial properties, but its role in Mycobacterium tuberculosis infection is unknown. We found that M. tuberculosis infection of human THP-1 monocytic cells induced the down-regulation of CatG mRNA express...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5712-5721.2004
更新日期:2004-10-01 00:00:00
abstract::Groups of female C57BL/6 and C3H/St mice were inoculated intraperitoneally (i.p.) with 10(9), 10(7), and 10(5) bacilli and into the right hind footpad with 10(7) and 10(5) bacilli of Mycobacterium lepraemurium. The incidence of death from leprosy and the mean survival time of leprous mice were recorded. In addition, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::We developed an efficient electrotransformation system for the pathogenic fungus Histoplasma capsulatum and used it to examine the effects of features of the transforming DNA on transformation efficiency and fate of the transforming DNA and to demonstrate fungal expression of two recombinant Escherichia coli genes, hp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1697-1707.1998
更新日期:1998-04-01 00:00:00
abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth, and these molecules induce an inflammatory response in the human host. The proinflammatory molecules include peptidoglycan monomers, peptidoglycan dimers, and free peptides. These molecules can be released by the actions of lytic transglycosylases o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00833-18
更新日期:2019-01-24 00:00:00
abstract::Microsporidia are obligate intracellular parasites that are ubiquitous in nature and have been recognized as causing an important emerging disease among immunocompromised individuals. Limited knowledge exists about the immune response against these organisms, and virtually nothing is known about the receptors involved...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00733-08
更新日期:2008-10-01 00:00:00
abstract::The attachment of radioisotope-labeled Mycoplasma pneumoniae to hamster tracheal rings in organ culture was examined by radioautography and liquid scintillation counting. Radioautographs of individual rings exposed for 8 h to (3H) thymidine-labeled virulent M. pneumoniae revealed a dense extracellular collection of em...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.959-966.1976
更新日期:1976-03-01 00:00:00
abstract::The effect of infection with low-virulence, tissue culture-propagated strains of reticuloendotheliosis virus (REV) on protective vaccinal immunity against Marek's disease (MD) lymphomas was investigated. Vaccinated chickens inoculated at hatching with greater than 10(4) focus-forming units of REV and challenged with M...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.90-98.1979
更新日期:1979-10-01 00:00:00
abstract::Arthritis is a frequent and major complication of infection with Borrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.658-663.2000
更新日期:2000-02-01 00:00:00
abstract::Relapsing fever is a rapidly progressive and severe septic disease caused by certain Borrelia spirochetes. The disease is divided into two forms, i.e., epidemic relapsing fever, caused by Borrelia recurrentis and transmitted by lice, and the endemic form, caused by several Borrelia species, such as B. duttonii, and tr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00007-06
更新日期:2006-07-01 00:00:00