The HMW1 and HMW2 Adhesins Enhance the Ability of Nontypeable Haemophilus influenzae To Colonize the Upper Respiratory Tract of Rhesus Macaques.

Abstract:

:Nontypeable Haemophilus influenzae (NTHi) initiates infection by colonizing the upper respiratory tract and is a common cause of localized respiratory tract disease. Previous work has established that the NTHi HMW1 and HMW2 proteins are potent adhesins that mediate efficient in vitro adherence to cultured human respiratory epithelial cells. In this study, we used a rhesus macaque model to assess the contributions of HMW1 and HMW2 to in vivo colonization. In experiments involving inoculation of individual isogenic derivatives of NTHi strain 12, the parent strain expressing both HMW1 and HMW2 and the mutant strains expressing either HMW1 or HMW2 were able to colonize more frequently than the double mutant strain lacking HMW1 and HMW2. In competition experiments, the parent strain efficiently outcompeted the double mutant lacking HMW1 and HMW2. Colonization with strains expressing HMW2 resulted in development of antibody against HMW2 in a number of the animals, demonstrating that colonization can stimulate an antibody response. In conclusion, we have established that the HMW1 and HMW2 adhesins play a major role in facilitating colonization of the upper respiratory tract of rhesus macaques, in some cases associated with stimulation of an immune response.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Rempe KA,Porsch EA,Wilson JM,St Geme JW 3rd

doi

10.1128/IAI.00153-16

subject

Has Abstract

pub_date

2016-09-19 00:00:00

pages

2771-8

issue

10

eissn

0019-9567

issn

1098-5522

pii

IAI.00153-16

journal_volume

84

pub_type

杂志文章
  • Lipopolysaccharide hyperreactivity of animals infected with Trypanosoma lewisi or Trypanosoma musculi.

    abstract::Rats and mice infected with Trypanosoma lewisi and Trypanosoma musculi, respectively, showed hyperreactivity to lipopolysaccharide (LPS) from gram-negative bacteria. Fatal shock could be precipitated with a dose of LPS 100 to 1,000 times less in infected compared with noninfected animals. In trypanosome-infected rats ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.46.2.501-506.1984

    authors: Ferrante A,Carter RF,Ferluga J,Allison AC

    更新日期:1984-11-01 00:00:00

  • Relationship of virulence factor expression to evolved virulence in mouse-passaged Cryptococcus neoformans lines.

    abstract::Serial passage of Cryptococcus neoformans in mice increases virulence relative to the nonpassaged line. Postpassaged lines showed no difference in the expression of most known virulence factors, with the exception that the more virulent lines had smaller capsules in vitro. These data imply that other mechanisms of vir...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.10.7047-7050.2005

    authors: McClelland EE,Perrine WT,Potts WK,Casadevall A

    更新日期:2005-10-01 00:00:00

  • Immunity to Escherichia coli in pigs: adhesion of enteropathogenic Escherichia coli to isolated intestinal epithelial cells.

    abstract::A method was developed to test for the ability of Escherichia coli to adhere to isolated intestinal epithelial cells. Of the E. coli tested, those having either K88ac or K88ab antigens adhered to the cells, and those which did not have these antigens did not. Since some enteropathogenic E. coli did not have the abilit...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.10.4.776-782.1974

    authors: Wilson MR,Hohmann AW

    更新日期:1974-10-01 00:00:00

  • Biosynthesis and functions of melanin in Sporothrix schenckii.

    abstract::Sporothrix schenckii is a human pathogen that causes sporotrichosis, an important cutaneous mycosis with a worldwide distribution. It produces dark-brown conidia, which infect the host. We found that S. schenckii synthesizes melanin via the 1,8-dihydroxynaphthalene pentaketide pathway. Melanin biosynthesis in the wild...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.6.3696-3703.2000

    authors: Romero-Martinez R,Wheeler M,Guerrero-Plata A,Rico G,Torres-Guerrero H

    更新日期:2000-06-01 00:00:00

  • Variation in chemical properties and antigenic determinants among type II heat-labile enterotoxins of Escherichia coli.

    abstract::Type II heat-labile enterotoxin (LT-II) from Escherichia coli 41 was purified and compared with prototype LT-II encoded by genes from E. coli SA53. Both toxins were oligomeric proteins consisting of polypeptides A (Mr, 28,000) and B (Mr, 11,800). The A polypeptides were cleaved by trypsin into fragments A1 (Mr, 21,000...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.54.2.529-536.1986

    authors: Guth BE,Twiddy EM,Trabulsi LR,Holmes RK

    更新日期:1986-11-01 00:00:00

  • Identification of a genetic locus essential for capsule sialylation in type III group B streptococci.

    abstract::The type III capsular polysaccharide of group B streptococci (GBS) consists of a linear backbone with short side chains ending in residues of N-acetylneuraminic acid, or sialic acid. The presence of sialic acid on the surface of the organism inhibits activation of the alternative pathway of complement and is thought t...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.2.392-400.1992

    authors: Wessels MR,Haft RF,Heggen LM,Rubens CE

    更新日期:1992-02-01 00:00:00

  • Differential Gamma Interferon- and Tumor Necrosis Factor Alpha-Driven Cytokine Response Distinguishes Acute Infection of a Metatherian Host with Toxoplasma gondii and Neospora caninum.

    abstract::Toxoplasma gondii and Neospora caninum (both Apicomplexa) are closely related cyst-forming coccidian parasites that differ significantly in their host ranges and ability to cause disease. Unlike eutherian mammals, Australian marsupials (metatherian mammals) have long been thought to be highly susceptible to toxoplasmo...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00173-17

    authors: Donahoe SL,Phalen DN,McAllan BM,O'Meally D,McAllister MM,Ellis J,Šlapeta J

    更新日期:2017-05-23 00:00:00

  • Susceptibility to reinfection after a primary chlamydial genital infection is associated with a decrease of antigen-specific T cells in the genital tract.

    abstract::We tested the hypothesis that the intensity of specific antichlamydial T cell-mediated immunity in the genital tract of female guinea pigs infected intravaginally with the chlamydial agent of guinea pig inclusion conjunctivitis would determine the resistance or susceptibility to reinfection after a primary chlamydial ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.4.1346-1351.1991

    authors: Igietseme JU,Rank RG

    更新日期:1991-04-01 00:00:00

  • Production and characterization of murine monoclonal antibodies to Histoplasma capsulatum yeast cell antigens.

    abstract::Four monoclonal antibodies (MAbs) were produced by immunizing mice with a disrupted yeast cell homogenate of Histoplasma capsulatum. MAbs 1 and 2 reacted only with the yeast cell antigens of H. capsulatum and Blastomyces dermatitidis, whereas MAbs 3 and 4 showed broader cross-reactivity. MAb 3 cross-reacted with B. de...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.3.896-901.1989

    authors: Kamel SM,Wheat LJ,Garten ML,Bartlett MS,Tansey MR,Tewari RP

    更新日期:1989-03-01 00:00:00

  • Role of mycobactin in the growth and virulence of tubercle bacilli.

    abstract::Tubercle bacilli failed to grow in iron-void media enriched with solutions of iron-containing transferrin (Tr) or ferritin (F) because these substances do not provide the bacilli with iron, which is essential for their growth. Animal serum and macrophages possessed no iron carrier with an ability to satisfy the need o...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.9.1.34-40.1974

    authors: Golden CA,Kochan I,Spriggs DR

    更新日期:1974-01-01 00:00:00

  • Both inducible nitric oxide synthase and NADPH oxidase contribute to the control of virulent phase I Coxiella burnetii infections.

    abstract::Host control of Coxiella burnetii infections is believed to be mediated primarily by activated monocytes/macrophages. The activation of macrophages by cytokines leads to the production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) that have potent antimicrobial activities. The contri...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.11.6666-6675.2004

    authors: Brennan RE,Russell K,Zhang G,Samuel JE

    更新日期:2004-11-01 00:00:00

  • Immunosuppressive agents in intracellular infection: besnoitiosis in hamsters.

    abstract::When tested for their activity in suppressing the acquisition of immunity during acute Besnoitia infection of hamsters, antilymphocyte serum (ALS), aminopterin, cyclophosphamide, cortisol, and whole-body irradiation were the most active agents and effectively blocked the development of immunity during 4 to 12 days of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.3.6.756-761.1971

    authors: Wilson HR,Frenkel JK

    更新日期:1971-06-01 00:00:00

  • A surface enolase participates in Borrelia burgdorferi-plasminogen interaction and contributes to pathogen survival within feeding ticks.

    abstract::Borrelia burgdorferi, a tick-borne bacterial pathogen, causes a disseminated infection involving multiple organs known as Lyme disease. Surface proteins can directly participate in microbial virulence by facilitating pathogen dissemination via interaction with host factors. We show here that a fraction of the B. burgd...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.05671-11

    authors: Nogueira SV,Smith AA,Qin JH,Pal U

    更新日期:2012-01-01 00:00:00

  • Bordetella pertussis binds the human complement regulator C4BP: role of filamentous hemagglutinin.

    abstract::C4BP (C4b-binding protein) is a high-molecular-weight plasma protein that inhibits the classical pathway of complement activation. Recent experiments have demonstrated that C4BP binds to many strains of the gram-positive bacterium Streptococcus pyogenes, a major respiratory tract pathogen. Binding to S. pyogenes was s...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.9.3638-3643.1997

    authors: Berggård K,Johnsson E,Mooi FR,Lindahl G

    更新日期:1997-09-01 00:00:00

  • Heparin inhibits phagocytosis by polymorphonuclear leukocytes.

    abstract::Phagocytosis of unopsonized Salmonella typhimurium 395, MR-10, opsonized Salmonella typhimurium 395 MS, and Staphylococcus epidermidis by rabbit polymorphonuclear leukocytes was inhibited by heparin at concentrations as low as 0.5 U/ml. Inhibition was dose dependent and nearly complete at 20 U/ml. Provided that hepari...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.32.1.295-299.1981

    authors: Victor M,Weiss J,Elsbach P

    更新日期:1981-04-01 00:00:00

  • Early events in phagosome establishment are required for intracellular survival of Legionella pneumophila.

    abstract::During infection, the Legionnaires' disease bacterium, Legionella pneumophila, survives and multiplies within a specialized phagosome that is near neutral pH and does not fuse with host lysosomes. In order to understand the molecular basis of this organism's ability to control its intracellular fate, we have isolated ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:

    authors: Wiater LA,Dunn K,Maxfield FR,Shuman HA

    更新日期:1998-09-01 00:00:00

  • Use of Moraxella catarrhalis lipooligosaccharide mutants to identify specific oligosaccharide epitopes recognized by human serum antibodies.

    abstract::Moraxella catarrhalis is a causative agent of otitis media in children and lower respiratory tract infections in adults suffering from chronic obstructive pulmonary disease (COPD). This strict human pathogen continues to be a significant cause of disease in this broad spectrum of patients because there is no available...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00294-09

    authors: Schwingel JM,Edwards KJ,Cox AD,Masoud H,Richards JC,St Michael F,Tekwe CD,Sethi S,Murphy TF,Campagnari AA

    更新日期:2009-10-01 00:00:00

  • Functional activities and epitope specificity of human and murine antibodies against the class 4 outer membrane protein (Rmp) of Neisseria meningitidis.

    abstract::Antibodies against the class 4 outer membrane protein (OMP) from Neisseria meningitidis have been purified from sera from vaccinees immunized with the Norwegian meningococcal group B outer membrane vesicle vaccine. The human sera and purified antibodies reacted strongly with the class 4 OMP in immunoblots, whereas exp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.3.1267-1276.1999

    authors: Rosenqvist E,Musacchio A,Aase A,Høiby EA,Namork E,Kolberg J,Wedege E,Delvig A,Dalseg R,Michaelsen TE,Tommassen J

    更新日期:1999-03-01 00:00:00

  • Localized expression of mRNA for phagocyte-specific chemotactic cytokines in human periodontal infections.

    abstract::In bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.9.4005-4014.1994

    authors: Tonetti MS,Imboden MA,Gerber L,Lang NP,Laissue J,Mueller C

    更新日期:1994-09-01 00:00:00

  • Genetic analysis of virulence plasmid from a serogroup 9 Yersinia enterocolitica strain: role of outer membrane protein P1 in resistance to human serum and autoagglutination.

    abstract::Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (70 kilobases) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of some outer membrane proteins (OMPs). To map the genes enco...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.3.782-786.1985

    authors: Balligand G,Laroche Y,Cornelis G

    更新日期:1985-06-01 00:00:00

  • Analysis of the human antibody response to thrombospondin-related anonymous protein of Plasmodium falciparum.

    abstract::Thrombospondin-related anonymous protein (TRAP) of the malaria parasite Plasmodium falciparum shares two sequence motifs with other proteins which possess adhesive properties. Recently, findings indicate that TRAP is an antigen which contributes to antisporozoite immunity. We have cloned and expressed the TRAP coding ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.8.3490-3495.1993

    authors: Scarselli E,Tolle R,Koita O,Diallo M,Müller HM,Früh K,Doumbo O,Crisanti A,Bujard H

    更新日期:1993-08-01 00:00:00

  • Intradermal infection model for pathogenesis and vaccine studies of murine visceral leishmaniasis.

    abstract::The levels of protection found in vaccine studies of murine visceral leishmaniasis are significantly lower than for cutaneous leishmaniasis; whether this is due to the high-challenge murine model employed and/or is a consequence of differences required in tissue-specific local immune responses is not understood. Conse...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.1.401-410.2003

    authors: Ahmed S,Colmenares M,Soong L,Goldsmith-Pestana K,Munstermann L,Molina R,McMahon-Pratt D

    更新日期:2003-01-01 00:00:00

  • Antagonistic effect of extremely oxygen-sensitive clostridia from the microflora of conventional mice and of Escherichia coli against Shigella flexneri in the digestive tract of gnotobiotic mice.

    abstract::Two extremely oxygen-sensitive strains of Clostridium sp., designated Clostridium E and P, were obtained from digestive microflora of conventional mice and found to constitute a barrier against Shigella flexneri SF-2 when associated in vivo with Escherichia coli K-12. These and other simplified fractions of the conven...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.17.2.415-424.1977

    authors: Ducluzeau R,Ladire M,Callut C,Raibaud P,Abrams GD

    更新日期:1977-08-01 00:00:00

  • Cryptosporidium parvum at different developmental stages modulates host cell apoptosis in vitro.

    abstract::We studied apoptosis in a human ileocecal adenocarcinoma tumor cell line (HCT-8) infected with Cryptosporidium parvum, from 2 to 72 h postinfection (h.p.i.). At 2 h.p.i., the percentage of annexin V-positive cells in the cell culture had increased to 10% compared to 2.5% in noninfected control culture; sorted infected...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.10.6061-6067.2004

    authors: Mele R,Gomez Morales MA,Tosini F,Pozio E

    更新日期:2004-10-01 00:00:00

  • Transfer of adoptive immunity to tuberculosis in mice.

    abstract::A system is described for studying adoptive immunity to tuberculosis in syngeneic mice. Donor mice were immunized with 10(4) BCG intravenously, and lymphoid cells were harvested 28 days later. Adoptive immunity was measured in recipient mice in terms of the inhibition of growth of BCG in the liver and spleen following...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.11.6.1174-1181.1975

    authors: Lefford MJ

    更新日期:1975-06-01 00:00:00

  • The major acute-phase protein, serum amyloid P component, in mice is not involved in endogenous resistance against tumor necrosis factor alpha-induced lethal hepatitis, shock, and skin necrosis.

    abstract::The proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha) induces lethal hepatitis when injected into D-(+)-galactosamine-sensitized mice on the one hand or systemic inflammatory response syndrome (SIRS) in normal mice on the other hand. We studied whether serum amyloid P component (SAP), the major acute-ph...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.9.5026-5029.2000

    authors: Van Molle W,Hochepied T,Brouckaert P,Libert C

    更新日期:2000-09-01 00:00:00

  • Electron microscope study of experimental enteric adenovirus infection in mice.

    abstract::Using fluorescent antibody techniques (FA) and light microscopy (LM) and electron microscopy (EM), this paper describes the morphological features of the ileum in the DK1 mouse orally challenged with adenovirus K87. At the peak of infection, virus is easily identified by FA in the epithelium of the villi and crypts of...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.13.2.569-580.1976

    authors: Takeuchi A,Hashimoto K

    更新日期:1976-02-01 00:00:00

  • Murine aerosol challenge model of anthrax.

    abstract::The availability of relevant and useful animal models is critical for progress in the development of effective vaccines and therapeutics. The infection of rabbits and non-human primates with fully virulent Bacillus anthracis spores provides two excellent models of anthrax disease. However, the high cost of procuring a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01875-06

    authors: Loving CL,Kennett M,Lee GM,Grippe VK,Merkel TJ

    更新日期:2007-06-01 00:00:00

  • Haemophilus influenzae resides and multiplies intracellularly in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay.

    abstract::The DNA oligomer 5'-d(TGCGGCCTCTCAGTCCCGCACTTTCATCTTCC)-3' specifically recognizes Haemophilus influenzae 16S rRNA. We report here the use of this oligonucleotide, with a fluorescein label tagged on its 5' end, as a probe for the in situ detection of nonencapsulated nontypeable H. influenzae in sections of adenoid tis...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.2.673-679.1994

    authors: Forsgren J,Samuelson A,Ahlin A,Jonasson J,Rynnel-Dagöö B,Lindberg A

    更新日期:1994-02-01 00:00:00

  • Inactivation of the srtA gene affects localization of surface proteins and decreases adhesion of Streptococcus pneumoniae to human pharyngeal cells in vitro.

    abstract::Inactivation of sortase gene srtA in Streptococcus pneumoniae strain R6 caused the release of beta-galactosidase and neuraminidase A (NanA) from the cell wall into the surrounding medium. Both of these surface proteins contain the LPXTG motif in the C-terminal domain. Complementation with plasmid-borne srtA reversed p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.5.2758-2765.2003

    authors: Kharat AS,Tomasz A

    更新日期:2003-05-01 00:00:00