Abstract:
:The guinea pig migration inhibition technique, an accepted in vitro correlate of delayed hypersensitivity, has been adapted to a murine system. Peritoneal exudate cells from CF-1 mice vaccinated with viable cells of the H37Ra strain of Mycobacterium tuberculosis were inhibited in vitro by purified protein derivative (PPD) or whole H37Ra microorganisms. Peritoneal exudate cells from the inbred C57Bl/6 mice immunized with H37Ra cells also were inhibited in vitro by PPD or whole H37Ra microorganisms. Migration inhibitory factor (MIF) was produced by splenic lymphocytes from the H37Ra-immunized C57Bl/6 mice when incubated with either antigen. Intravenous injection of PPD or viable H37Ra organisms into H37Ra mice resulted in MIF production in vitro by splenic lymphocytes without further antigenic stimulation. Peritoneal exudate cells from nonimmunized C57Bl/6 mice and supernatant fluids from cultures of lymphocytes from nonimmunized C57Bl/6 mice were not inhibited in the presence of antigen. The production of MIF by splenic lymphocytes from immunized C57Bl/6 mice depended upon the conditions under which the lymphocytes were cultured, the time of exposure to antigen (3 days), the use of a higher concentration of PPD for stimulation of lymphocytes than that required for guinea pig cells, and also the use of cells from a highly inbred mouse strain.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Neiburger RG,Youmans GPdoi
10.1128/IAI.7.2.190-195.1973subject
Has Abstractpub_date
1973-02-01 00:00:00pages
190-5issue
2eissn
0019-9567issn
1098-5522journal_volume
7pub_type
杂志文章abstract::Reverse transcriptase PCR analyses have demonstrated that open reading frames (ORFs) PM0298, PM0299, and PM0300 of the animal pathogen Pasteurella multocida constitute a single transcriptional unit. By cloning and overexpression studies in Escherichia coli cells, the product of ORF PM0300 was shown to bind hemoglobin ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.5955-5964.2002
更新日期:2002-11-01 00:00:00
abstract::Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1420-1425.1996
更新日期:1996-04-01 00:00:00
abstract::Enteropathogenic Escherichia coli (EPEC) interacts with intestinal epithelial cells, activating host signaling pathways leading to cytoskeletal rearrangements and ultimately diarrhea. In this study, we demonstrate that EPEC interacts with the macrophage-like cell line J774A.1 to inhibit phagocytosis by these cells. An...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.490-495.1999
更新日期:1999-02-01 00:00:00
abstract::Experimental infections of mice with the African trypanosome Trypanosoma brucei lead to a profound state of T-cell unresponsiveness in the lymph node cell (LNC) compartment. This suppression is mediated by macrophage-like cells which inhibit interleukin 2 (IL-2) secretion and down-regulate IL-2 receptor expression (M....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.1937-1943.1996
更新日期:1996-06-01 00:00:00
abstract::Although excystation is crucial to the initiation of infection by Giardia lamblia, little is known about the regulation of this important process. We have been able to reliably induce excystation in vitro by mimicking cyst passage through the stomach and upper small intestine by the exposure of in vitro-derived cysts ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2151-2157.1996
更新日期:1996-06-01 00:00:00
abstract::Purified Escherichia coli type 1 fimbriae have been shown previously to stimulate T-cell-independent proliferation of human B lymphocytes. The response is mediated by the mannose-specific, lectin-like adhesin protein FimH. Here we show that type 1 fimbriae also stimulate immunoglobulin (Ig) secretion by B cells. The r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5197-5203.1992
更新日期:1992-12-01 00:00:00
abstract::Organisms of the Mycobacterium avium complex are associated with disseminated infection in patients with AIDS. The mechanisms that account for the survival of the intracellular bacteria are unknown. We document here that infection of C57BL/6 black mice with M. avium 101 triggered interleukin-10 (IL-10) production. The...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.7.3093-3097.1993
更新日期:1993-07-01 00:00:00
abstract::Hamster (Mesocricetus auratus) neutrophil granules contain at least four microbicidal peptides belonging to the defensin family. These compounds were purified from granule acid extracts by reverse-phase chromatography and termed HaNP-1 to -4 (hamster neutrophil peptide). HaNP-1 and HaNP-3 revealed the most bactericida...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4444-4449.1996
更新日期:1996-11-01 00:00:00
abstract::We have developed a rapid, continuous method for real-time monitoring of biofilms, both in vitro and in a mouse infection model, through noninvasive imaging of bioluminescent bacteria colonized on Teflon catheters. Two important biofilm-forming bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.882-890.2003
更新日期:2003-02-01 00:00:00
abstract::The primary effector for Shigella invasion of epithelial cells is IpaC, which is secreted via a type III secretion system. We recently reported that the IpaC N terminus is required for type III secretion and possibly other functions. In this study, mutagenesis was used to identify an N-terminal secretion signal and to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1255-1264.2003
更新日期:2003-03-01 00:00:00
abstract::Gamma interferon (IFN-gamma) is produced in response to circulating lipopolysaccharide (LPS) and contributes to the lethality of endotoxic shock. To address the cellular source of IFN-gamma production in vivo, T cells and B cells were magnetically purified from C57BL/6 mouse spleens 5 h following endotoxin injection. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4244-4249.1994
更新日期:1994-10-01 00:00:00
abstract::The effect of human-specific antibody on surface membrane antigens of trypomastigotes of Trypanosoma cruzi was studied in vitro by using immunofluorescence methods. Immune sera induced aggregation of surface antigens in trypomastigotes to form polar cell caps. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.567-569.1978
更新日期:1978-05-01 00:00:00
abstract::Attachment and ingestion of Mycobacterium avium subsp. paratuberculosis by two epithelial cell lines were enhanced by soluble fibronectin (FN). Peptide blocking of the FN attachment protein (FAP-P) inhibited the internalization of M. avium subsp. paratuberculosis. Disruption of FAP-P expression significantly reduced a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2670-2675.2002
更新日期:2002-05-01 00:00:00
abstract::Enteroaggregative Escherichia coli (EAEC) is an emerging cause of diarrheal illness. Clinical data suggest that diarrhea caused by EAEC is predominantly secretory in nature, but the responsible enterotoxin has not been described. Work from our laboratories has implicated a ca. 108-kDa protein as a heat-labile enteroto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3155-3163.1998
更新日期:1998-07-01 00:00:00
abstract::Mice orally immunized with Salmonella dublin EL23, a nonreverting, aromatic-dependent, histidine-requiring mutant transformed with a plasmid which carries a gene that codes for production of the B subunit of the heat-labile toxin (LT-B) of enterotoxigenic Escherichia coli, or with purified LT-B alone were compared for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.1076-1083.1995
更新日期:1995-03-01 00:00:00
abstract::Macrophages can undergo apoptosis after infection with Mycobacterium tuberculosis. This macrophage response deprives the bacillus of its niche cell and supports the host response through better antigen presentation. The intracellular pathways of apoptosis that elaborate this macrophage response are not well understood...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01107-06
更新日期:2007-04-01 00:00:00
abstract::The antigenically conserved proteins of Treponema pallidum subsp. pallidum and four nonpathogenic cultivatable treponemes were investigated by phase partitioning with the nonionic detergent Triton X-114 and immunoblot analysis. None of the T. pallidum integral membrane proteins identified by phase partitioning (deterg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1825-1828.1988
更新日期:1988-07-01 00:00:00
abstract::Immunoglobulin A (IgA) was prepared from samples of parotid saliva collected from two individuals over a 150-day period. Standardized solutions of the IgA were tested for agglutinating antibody activity against strains of streptococcus salivarius, S. sanguis, S. mitis, and S. mutans that had been isolated from a homol...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.603-606.1975
更新日期:1975-03-01 00:00:00
abstract::Suppression of the host defense is one of the hallmarks of Yersinia enterocolitica infection. This enteric pathogen resists phagocytosis and interferes with macrophage functions from an extracellular localization (oxidative-burst generation and tumor necrosis factor alpha production). In this study, we investigated th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4813-4821.1997
更新日期:1997-11-01 00:00:00
abstract::Human immunodeficiency virus (HIV)-infected individuals are predisposed to recurrent oral candidiasis, and, although it has been assumed that this is because of deficient mucosal immune responses, this has not been properly established. The present study aimed to compare the concentrations and secretion rates of immun...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.892-896.1994
更新日期:1994-03-01 00:00:00
abstract::The short-term kinetics of fluoride uptake by cells from 20- to 22-h cultures of Streptococcus mutans strain 6715 were studied using rapid filtration and centrifugation techniques. Saline-suspended organisms were diluted with fluoride-containing solutions buffered at four different pH values (2.0, 4.0, 5.5, and 8.2). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.680-687.1977
更新日期:1977-12-01 00:00:00
abstract::The 70-kilobase pYV plasmid of Yersinia enterocolitica encodes a set of proteins called Yops that are produced during infection. To use Y. enterocolitica as a live carrier to present the cholera toxin B (CT-B) subunit to the immune system, we constructed an operon fusion between ctxB and the yop51 gene. This operon fu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2420-2428.1990
更新日期:1990-08-01 00:00:00
abstract::Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high proportion of intravenously immunized mice survived virulent challenge, but subcutaneous vaccination was less effective. Vac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.798-803.1978
更新日期:1978-12-01 00:00:00
abstract::Accurate diagnosis and early treatment of tuberculosis (TB) and latent TB infection (LTBI) are vital to prevent and control TB. The lack of specific biomarkers hinders these efforts. This study's purpose was to screen immunological markers that discriminate Mycobacterium tuberculosis infection outcomes in a setting wh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00759-17
更新日期:2018-03-22 00:00:00
abstract::Since we previously observed a remarkable decrease in the numbers of T cells in the Peyer's patches of the small intestines in C57BL/6 mice following peroral infection with Toxoplasma gondii, we performed studies to examine the mechanism(s) whereby this decrease in numbers of the T cells occurs. We found that apoptoti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4682-4689.1997
更新日期:1997-11-01 00:00:00
abstract::After uptake and retrograde transport pertussis toxin acts by ADP-ribosylating alpha-Gi proteins. We show that uptake via many different receptor proteins followed by retrograde transport and intoxication is not restricted to a particular cell type. The efficiency of cellular intoxication, however, was found to be cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.433-435.1999
更新日期:1999-01-01 00:00:00
abstract::The antigenic cross-reactivity between Treponema pallidum and several pathogenic members of the family Spirochaetaceae was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques. Blots of T. pallidum antigens were incubated with antiserum from rabbits infected or immunize...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.116-121.1984
更新日期:1984-10-01 00:00:00
abstract::It was shown that a strain of Serratia liquefaciens harbors a conjugative R-plasmid responsible for reistance to the following 14 antibiotics: ampicillin, carbenicillin, cephalothin, butirosin, neomycin, paramomycin, kanamycin, lividomycin, gentamicin, tobramycin, streptomycin, tetracycline, sulfonamide, and chloramph...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Mice infected with Trypanosoma musculi developed hyperplasia of the spleen, lymph nodes, and liver; in contrast, their thymuses displayed transient involution. All organs returned to normal in a month or less. There was modest anemia, lasting until the parasites were cleared from the bloodstream, followed by a rapid i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.1008-1017.1981
更新日期:1981-12-01 00:00:00
abstract::Cryptococcus neoformans NIH 409 was cultured in a defined medium containing D-[1-13C]xylose (Xyl), D-[1-13C]mannose (Man), or D-[1-13C]mannitol as the sole carbon source. The distribution of 13C in the Man, Xyl, glucuronic acid (GlcA), and O-acetyl constituents of native and de-O-acetylated glucuronoxylomannan (GXM) w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2996-2998.1998
更新日期:1998-06-01 00:00:00