Burkholderia (basonym Pseudomonas) cepacia binding to lipid receptors.


:Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was also identified on cells and appeared to serve as the major cell receptor for poorly piliated or nonpiliated isolates. In the present study in vitro approaches were used to identify putative lipid receptors for B. cepacia and to explore the nature of the binding interaction. As judged by thin-layer chromatography overlay assays, the best receptors were digalactosylceramide and globotriosylceramide (Gb(3)). Both contain and unsubstituted terminal Gal alpha 1-4Gal sequence. B cepacia also bound moderately to galactosylceramide, gangliotriosylceramide, and gangliotetraosylceramide. Binding to glycolipids was not affected by tetramethylurea, a hydrophobic-bond-breaking adhesin for GB(3). Binding to glycolipids was not affected by tetramethylurea, a hydrophobic-bond-breaking agent. Binding was influenced by the structure of the ceramide, which probably affects the presentation of the agent. Binding was influenced by the structure of the ceramide, which probably affects the presentation of the carbohydrate epitope to the bacteria. Gb(3) was also the major receptor in lipid extracts of human erythrocytes, human buccal epithelial cells and HEp-2 laryngeal epithelial cells. In a receptor-based enzyme-linked immunosorbent assay, binding to Gb(3) within a phospholipid-cholesterol mixture (a membrane-like environment) increased and then approached saturation as a direct function of increasing bacterial concentration. The calculated value of K(a) (3.06 X 10(-8) ml/CFU), the affinity constant, was almost identical to the K(a) calculated earlier for B. cepacia binding to a set of lipid receptors in buccal epithelial cells (1.5 X 10(-8) to 2.0 X 10(-8) ml/CFU). Our findings suggest that within cell membranes, galactose-containing glycolipids, particularly Gb(3) are good candidates for receptors for B. cepacia, particularly for isolates in which cable pili are poorly expressed.


Infect Immun


Infection and immunity


Sylvester FA,Sajjan US,Forstner JF




Has Abstract


1996-04-01 00:00:00












  • Characterization of an 18,000-molecular-weight outer membrane protein of Haemophilus ducreyi that contains a conserved surface-exposed epitope.

    abstract::Identification of antigenically conserved surface components of Haemophilus ducreyi may facilitate the development of reagents to diagnose and prevent chancroid. A hybridoma derived from a mouse immunized with nontypeable Haemophilus influenzae produced a monoclonal antibody (MAb), designated 3B9, that bound to 35 of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Spinola SM,Griffiths GE,Bogdan J,Menegus MA

    更新日期:1992-02-01 00:00:00

  • Antibodies produced by rabbits immunized with visna virus.

    abstract::Immunization of New Zealand White rabbits with purified visna virus elicited antibody activity demonstrated by passive hemagglutination (PHA), complement fixation (CF), and indirect immunofluorescent tests. The antibody activities of hyperimmune sera and Sephadex G-200 fractions of the sera were studied. It was found ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Karl SC,Thormar H

    更新日期:1971-12-01 00:00:00

  • Experimental model of congenital syphilis.

    abstract::Female LSH hamsters infected with Treponema pallidum subsp, endemicum before pregnancy or during early pregnancy transmit a form of syphilis to the fetus that is similar to human congenital syphilis. The offspring develops rhinitis, skin rash, failure to thrive, and hepatosplenomegaly. T. pallidum is detectable in the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kajdacsy-Balla A,Howeedy A,Bagasra O

    更新日期:1993-08-01 00:00:00

  • Proteophosphoglycan, a major secreted product of intracellular Leishmania mexicana amastigotes, is a poor B-cell antigen and does not elicit a specific conventional CD4+ T-cell response.

    abstract::Secreted and surface-exposed antigens of intracellular pathogens are thought to provide target structures for detection by the host immune system. The major secreted product of intracellular Leishmania mexicana amastigotes, a proteophosphoglycan (aPPG), is known to contribute to the establishment of the parasitophorou...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Aebischer T,Harbecke D,Ilg T

    更新日期:1999-10-01 00:00:00

  • Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice.

    abstract::This study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion DNA vaccine encoding both the Brucella abortus L7/L12 protein (ribosomal protein) and Omp16 protein (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection of this divalent DNA vaccin...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Luo D,Ni B,Li P,Shi W,Zhang S,Han Y,Mao L,He Y,Wu Y,Wang X

    更新日期:2006-05-01 00:00:00

  • Detection of coronavirus strain 692 by immune electron microscopy.

    abstract::Utilization of the technique of immune electron microscopy has enabled the detection of a coronavirus in organ culture harvests derived from a washing from an adult with an acute upper respiratory tract illness; convalescent serum was the source of specific antibody. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kapikian AZ,James HD,Kelly SJ,Vaughn AL

    更新日期:1973-01-01 00:00:00

  • FlhA, a component of the flagellum assembly apparatus of Pseudomonas aeruginosa, plays a role in internalization by corneal epithelial cells.

    abstract::Pseudomonas aeruginosa invades various epithelial cell types in vitro and in vivo. The P. aeruginosa genome possesses a gene (flhA) which encodes a protein that is believed to be part of the export apparatus for flagellum assembly and which is homologous to invA of Salmonella spp. Because invA is required for invasion...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fleiszig SM,Arora SK,Van R,Ramphal R

    更新日期:2001-08-01 00:00:00

  • Nonfunctional variant 3 factor H binding proteins as meningococcal vaccine candidates.

    abstract::Neisseria meningitidis is a human-specific pathogen and leading cause of meningitis and septicemia. Factor H binding protein (fHbp), a virulence factor which protects N. meningitidis from innate immunity by binding the human complement regulator factor H (fH) with high affinity, is also a key antigen in vaccines being...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: van der Veen S,Johnson S,Jongerius I,Malik T,Genovese A,Santini L,Staunton D,Ufret-Vincenty RL,Pickering MC,Lea SM,Tang CM

    更新日期:2014-03-01 00:00:00

  • Epidermal Growth Factor Receptor and Transforming Growth Factor β Signaling Pathways Cooperate To Mediate Chlamydia Pathogenesis.

    abstract::Human genital Chlamydia infection is a major public health concern due to the serious reproductive system complications. Chlamydia binds several receptor tyrosine kinases (RTKs) on host cells, including the epidermal growth factor receptor (EGFR), and activates cellular signaling cascades for host invasion, cytoskelet...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Igietseme JU,Partin J,George Z,Omosun Y,Goldstein J,Joseph K,Ellerson D,Eko FO,Pohl J,Bandea C,Black CM

    更新日期:2020-03-23 00:00:00

  • Localization of immunogenic regions on the flagellin proteins of Campylobacter jejuni 81116.

    abstract::The purpose of this study was to localize antigenic regions on the flagellin protein of Campylobacter jejuni 81116. This strain has two flagellin genes, flaA and flaB, which are 95% identical; only flaA seems to be expressed in motile C. jejuni 81116 cells. Fragments of flaA and flaB were cloned in the bacterial expre...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nuijten PJ,van der Zeijst BA,Newell DG

    更新日期:1991-03-01 00:00:00

  • Antibody-mediated protection against infection with Helicobacter pylori in a suckling mouse model of passive immunity.

    abstract::Studies of active immunization against Helicobacter pylori indicate that antibodies play a minor role in immunity. There is also evidence, however, that the translocation of antibodies in the stomach may be insufficient to achieve functional antibody levels in the gastric lumen. We have used a suckling mouse model of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gorrell RJ,Robins-Browne RM

    更新日期:2009-11-01 00:00:00

  • Comparison of candidacidal and candidastatic activities of human neutrophils.

    abstract::Disruption of neutrophils causes the release of a cytoplasmic protein which can inhibit the growth of Candida albicans without killing the organisms. The present study was undertaken with a combined system in which candidacidal and candidastatic activities of human neutrophils could be compared. In this system it was ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sohnle PG,Collins-Lech C

    更新日期:1990-08-01 00:00:00

  • Capsular polysaccharide of Cryptococcus neoformans induces proinflammatory cytokine release by human neutrophils.

    abstract::Human polymorphonuclear leukocytes from normal subjects produced proinflammatory cytokines in response to stimulation with Cryptococcus neoformans yeast cells. The cytokines released after stimulation of neutrophils included interleukin-1beta (IL-1beta), IL-6, IL-8 and tumor necrosis factor alpha. The magnitude of the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Retini C,Vecchiarelli A,Monari C,Tascini C,Bistoni F,Kozel TR

    更新日期:1996-08-01 00:00:00

  • Ehrlichia chaffeensis and Anaplasma phagocytophilum lack genes for lipid A biosynthesis and incorporate cholesterol for their survival.

    abstract::Ehrlichia chaffeensis and Anaplasma phagocytophilum are agents of human monocytic and granulocytic ehrlichioses, respectively. They are extremely sensitive to mechanical stress and are pleomorphic gram-negative bacteria. Membrane incorporation of cholesterol from the eukaryotic host is known to be essential for other ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lin M,Rikihisa Y

    更新日期:2003-09-01 00:00:00

  • Eosinophils can function as antigen-presenting cells to induce primary and secondary immune responses to Strongyloides stercoralis.

    abstract::Several studies have demonstrated roles for eosinophils during innate and adaptive immune responses to helminth infections. However, evidence that eosinophils are capable of initiating an immune response to parasite antigens is lacking. The goal of the present in vitro study was to investigate the potential of eosinop...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Padigel UM,Lee JJ,Nolan TJ,Schad GA,Abraham D

    更新日期:2006-06-01 00:00:00

  • Passively released heme from hemoglobin and myoglobin is a potential source of nutrient iron for Bordetella bronchiseptica.

    abstract::Colonization by Bordetella bronchiseptica results in a variety of inflammatory respiratory infections, including canine kennel cough, porcine atrophic rhinitis, and a whooping cough-like disease in humans. For successful colonization, B. bronchiseptica must acquire iron (Fe) from the infected host. A vast amount of Fe...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mocny JC,Olson JS,Connell TD

    更新日期:2007-10-01 00:00:00

  • Stx2-specific human monoclonal antibodies protect mice against lethal infection with Escherichia coli expressing Stx2 variants.

    abstract::Shiga toxin-producing Escherichia coli (STEC) strains are responsible for causing hemolytic-uremic syndrome (HUS), and systemic administration of Shiga toxin (Stx)-specific human monoclonal antibodies (HuMAbs) is considered a promising approach for prevention or treatment of the disease in children. The goal of the pr...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sheoran AS,Chapman S,Singh P,Donohue-Rolfe A,Tzipori S

    更新日期:2003-06-01 00:00:00

  • Reconstitution of an active arginine deiminase pathway in Mycoplasma pneumoniae M129.

    abstract::Some species of the genus Mycoplasma code for the arginine deiminase pathway (ADI), which enables these bacteria to produce ATP from arginine by the successive reaction of three enzymes: arginine deiminase (ArcA), ornithine carbamoyltransferase (ArcB), and carbamate kinase (ArcC). It so far appears that independently ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rechnitzer H,Rottem S,Herrmann R

    更新日期:2013-10-01 00:00:00

  • Regulation of mycobacterial growth by the hypothalamus-pituitary-adrenal axis: differential responses of Mycobacterium bovis BCG-resistant and -susceptible mice.

    abstract::The role of the hypothalamus-pituitary-adrenal (HPA) axis in regulating the growth of Mycobacterium avium in Mycobacterium bovis BCG-resistant and -susceptible congenic mice was evaluated. Restraint was used to activate the HPA axis, which resulted in an increase in the level of corticosterone in the plasma. Activatio...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Brown DH,Sheridan J,Pearl D,Zwilling BS

    更新日期:1993-11-01 00:00:00

  • Early events in phagosome establishment are required for intracellular survival of Legionella pneumophila.

    abstract::During infection, the Legionnaires' disease bacterium, Legionella pneumophila, survives and multiplies within a specialized phagosome that is near neutral pH and does not fuse with host lysosomes. In order to understand the molecular basis of this organism's ability to control its intracellular fate, we have isolated ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wiater LA,Dunn K,Maxfield FR,Shuman HA

    更新日期:1998-09-01 00:00:00

  • Plasmid-cured Salmonella enteritidis AL1192 as a candidate for a live vaccine.

    abstract::We report the immunizing capacity of Salmonella enteritidis AL1192, a strain that has been cured of a 36-megadalton plasmid, to protect ddY mice against subsequent challenge with virulent salmonellas. This strain, which was given subcutaneously at a dose of 10(6) organisms, provided significant protection against oral...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nakamura M,Sato S,Ohya T,Suzuki S,Ikeda S,Koeda T

    更新日期:1985-11-01 00:00:00

  • Borrelia burgdorferi spirochetes induce mast cell activation and cytokine release.

    abstract::The Lyme disease spirochete, Borrelia burgdorferi, is introduced into human hosts via tick bites. Among the cell types present in the skin which may initially contact spirochetes are mast cells. Since spirochetes are known to activate a variety of cell types in vitro, we tested whether B. burgdorferi spirochetes could...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Talkington J,Nickell SP

    更新日期:1999-03-01 00:00:00

  • Adhesion of Streptococcus pneumoniae to human pharyngeal epithelial cells in vitro: differences in adhesive capacity among strains isolated from subjects with otitis media, septicemia, or meningitis or from healthy carriers.

    abstract::A method was developed to study the adhesion of Streptococcus pneumoniae to human pharyngeal epithelial cells. Epithelial cells from healthy persons, pneumococcal strains from patients with otitis media, meningitis, or septicemia, and pneumococcal cells from the nasopharynx of healthy carriers were used. Adhesion was ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Andersson B,Eriksson B,Falsen E,Fogh A,Hanson LA,Nylén O,Peterson H,Svanborg Edén C

    更新日期:1981-04-01 00:00:00

  • Characterization of murine monoclonal and murine, rabbit, and human polyclonal antibodies against chlamydial lipopolysaccharide.

    abstract::Murine monoclonal and rabbit, murine, and human polyclonal antibodies against chlamydial lipopolysaccharide (LPS) were characterized by the passive hemolysis and passive hemolysis inhibition assays and by absorption experiments with LPSs of Chlamydia psittaci, Chlamydia trachomatis, and a recombinant strain of Salmone...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Brade L,Holst O,Kosma P,Zhang YX,Paulsen H,Krausse R,Brade H

    更新日期:1990-01-01 00:00:00

  • Combined effects of blood and temperature shift on Borrelia burgdorferi gene expression as determined by whole genome DNA array.

    abstract::Borrelia burgdorferi undergoes differential gene expression during transmission from its tick vector to a vertebrate host. The addition of blood to a spirochete culture at 35 degrees C for 48 h had a dramatic effect on gene expression of this organism. Utilizing B. burgdorferi whole genome DNA arrays, we compared the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tokarz R,Anderton JM,Katona LI,Benach JL

    更新日期:2004-09-01 00:00:00

  • Sigma B contributes to Listeria monocytogenes gastrointestinal infection but not to systemic spread in the guinea pig infection model.

    abstract::Contributions of the alternative sigma factor sigmaB to Listeria monocytogenes infection were investigated using strains bearing null mutations in sigB, prfA, or inlA or in selected inlA or prfA promoter regions. The DeltaP4inlA strain, which has a deletion in the sigmaB-dependent P4inlA promoter, and the DeltasigB st...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Garner MR,Njaa BL,Wiedmann M,Boor KJ

    更新日期:2006-02-01 00:00:00

  • High-molecular-weight polysaccharide antigen from Pseudomonas aeruginosa immunotype 2.

    abstract::Previously, we isolated a high-molecular-weight immunogenic polysaccharide (designated PS) from Pseudomonas aeruginosa immunotype 1 (IT-1). The method which we used was modified to permit the isolation of a similar PS from P. aeruginosa IT-2. This antigen was composed primarily of carbohydrate, had a complex monosacch...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pier GB,Sidberry HF,Sadoff JC

    更新日期:1981-11-01 00:00:00

  • Immune responses in infections with coccidia: macrophage activity.

    abstract::Peritoneal exudate cells from chickens immunized with two species of coccidia, Eimeria tenella or Eimeria maxima, were examined for their capacity to phagocytose stages of the parasite in vitro. True phagocytosis of the sporozoite stage is difficult to estimate because of its ability to invade cells, but may be evalua...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rose ME

    更新日期:1974-10-01 00:00:00

  • Analysis of a heme-dependent signal transduction system in Corynebacterium diphtheriae: deletion of the chrAS genes results in heme sensitivity and diminished heme-dependent activation of the hmuO promoter.

    abstract::The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is involved in the utilization of heme as an iron source. Transcription of hmuO is activated by heme or hemoglobin and repressed by iron and DtxR. Previous studies with Escherichia coli showed that heme-dependent transcriptional activation of an h...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bibb LA,King ND,Kunkle CA,Schmitt MP

    更新日期:2005-11-01 00:00:00

  • Interferon administered in the cerebrospinal space and its effect on rabies in rabbits.

    abstract::Because combined administration of intramuscular and intravenous interferon has been partially successful in the incubationary treatment of rabies, the effect of direct interferon administration into the cerebrospinal fluid space was tested. After injecting 1,800 U of interferon into the cisterna magna or the lateral ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ho M,Nash C,Morgan CW,Armstrong JA,Carroll RG,Postic B

    更新日期:1974-02-01 00:00:00