Burkholderia (basonym Pseudomonas) cepacia binding to lipid receptors.


:Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was also identified on cells and appeared to serve as the major cell receptor for poorly piliated or nonpiliated isolates. In the present study in vitro approaches were used to identify putative lipid receptors for B. cepacia and to explore the nature of the binding interaction. As judged by thin-layer chromatography overlay assays, the best receptors were digalactosylceramide and globotriosylceramide (Gb(3)). Both contain and unsubstituted terminal Gal alpha 1-4Gal sequence. B cepacia also bound moderately to galactosylceramide, gangliotriosylceramide, and gangliotetraosylceramide. Binding to glycolipids was not affected by tetramethylurea, a hydrophobic-bond-breaking adhesin for GB(3). Binding to glycolipids was not affected by tetramethylurea, a hydrophobic-bond-breaking agent. Binding was influenced by the structure of the ceramide, which probably affects the presentation of the agent. Binding was influenced by the structure of the ceramide, which probably affects the presentation of the carbohydrate epitope to the bacteria. Gb(3) was also the major receptor in lipid extracts of human erythrocytes, human buccal epithelial cells and HEp-2 laryngeal epithelial cells. In a receptor-based enzyme-linked immunosorbent assay, binding to Gb(3) within a phospholipid-cholesterol mixture (a membrane-like environment) increased and then approached saturation as a direct function of increasing bacterial concentration. The calculated value of K(a) (3.06 X 10(-8) ml/CFU), the affinity constant, was almost identical to the K(a) calculated earlier for B. cepacia binding to a set of lipid receptors in buccal epithelial cells (1.5 X 10(-8) to 2.0 X 10(-8) ml/CFU). Our findings suggest that within cell membranes, galactose-containing glycolipids, particularly Gb(3) are good candidates for receptors for B. cepacia, particularly for isolates in which cable pili are poorly expressed.


Infect Immun


Infection and immunity


Sylvester FA,Sajjan US,Forstner JF




Has Abstract


1996-04-01 00:00:00












  • Evaluation of phase variation of nontypeable Haemophilus influenzae lipooligosaccharide during nasopharyngeal colonization and development of otitis media in the chinchilla model.

    abstract::Nontypeable Haemophilus influenzae (NTHI) has four loci, lic-1 to lic-3 and lgtC, that generate phase-variable lipooligosaccharide (LOS) structures. lic-1, which is required for the expression of phosphorylcholine (ChoP), is the best characterized and is associated with an enhanced ability of H. influenzae to persist ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tong HH,Blue LE,James MA,Chen YP,DeMaria TF

    更新日期:2000-08-01 00:00:00

  • Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via Toll-like receptor 2 activation in the intestinal mucosa.

    abstract::The Toll-like receptor 2 (TLR2)/TLR1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. To determine whether this TLR2/TLR1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, w...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nishimori JH,Newman TN,Oppong GO,Rapsinski GJ,Yen JH,Biesecker SG,Wilson RP,Butler BP,Winter MG,Tsolis RM,Ganea D,Tükel Ç

    更新日期:2012-12-01 00:00:00

  • Regulatory Interactions among adhesin gene systems of uropathogenic Escherichia coli.

    abstract::Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identit...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lindberg S,Xia Y,Sondén B,Göransson M,Hacker J,Uhlin BE

    更新日期:2008-02-01 00:00:00

  • Interaction of Chlamydia trachomatis organisms and HeLa 229 cells.

    abstract::The infection of HeLa 229 cells in monolayer culture with trachoma (B/TW-5/OT) and lymphogranuloma venereum (LGV) (L2/434/Bu) organism was studied in terms of two parameters: radioactivity counts of cell-associated tritium labeled organisms at the initial stage of inoculation for measurement of attachment, and inclusi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kuo CC,Grayston T

    更新日期:1976-04-01 00:00:00

  • The chlamydial plasmid-encoded protein pgp3 is secreted into the cytosol of Chlamydia-infected cells.

    abstract::The chlamydial cryptic plasmid encodes eight putative open reading frames (ORFs), designated pORF1 to -8. Antibodies raised against these ORF proteins were used to localize the endogenous proteins during chlamydial infection. We found that the pORF5 protein (also known as pgp3) was detected mainly in the cytosol of Ch...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Li Z,Chen D,Zhong Y,Wang S,Zhong G

    更新日期:2008-08-01 00:00:00

  • Salmonella enterica Serovar Typhi Lipopolysaccharide O-Antigen Modification Impact on Serum Resistance and Antibody Recognition.

    abstract::Salmonella enterica serovar Typhi is a human-restricted Gram-negative bacterial pathogen responsible for causing an estimated 27 million cases of typhoid fever annually, leading to 217,000 deaths, and current vaccines do not offer full protection. The O-antigen side chain of the lipopolysaccharide is an immunodominant...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kintz E,Heiss C,Black I,Donohue N,Brown N,Davies MR,Azadi P,Baker S,Kaye PM,van der Woude M

    更新日期:2017-03-23 00:00:00

  • Membrane glycoprotein M-2 protects against Leishmania amazonensis infection.

    abstract::Previous passive antibody transfer experiments have indicated that immunity to a 46-kilodalton membrane glycoprotein (M-2) of Leishmania amazonensis may protect against infection with this parasite. In the studies described in this paper, we investigated the ability of the purified M-2 molecule to elicit a protective ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Champsi J,McMahon-Pratt D

    更新日期:1988-12-01 00:00:00

  • YAPI, a new Yersinia pseudotuberculosis pathogenicity island.

    abstract::Pathogenicity islands (PAIs) are chromosomal clusters of pathogen-specific virulence genes often found at tRNA loci. In the Yersinia pseudotuberculosis 32777 chromosome, we characterized a 98-kb segment that has all of the characteristic features of a PAI, including insertion in a (phenylalanine) tRNA gene, the presen...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Collyn F,Billault A,Mullet C,Simonet M,Marceau M

    更新日期:2004-08-01 00:00:00

  • Toll-like receptor 2 mediates alveolar macrophage response to Pneumocystis murina.

    abstract::The innate immune response to Pneumocystis infection is not well understood. In this study, normal C57BL/6 mouse alveolar macrophages were found to respond to Pneumocystis murina organisms through Toll-like receptor 2 (TLR2), leading to the nuclear translocation of NF-kappaB and the production of proinflammatory cytok...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Zhang C,Wang SH,Lasbury ME,Tschang D,Liao CP,Durant PJ,Lee CH

    更新日期:2006-03-01 00:00:00

  • Purification of dermonecrotic toxin from a sonic extract of Pasteurella multocida SP-72 serotype D.

    abstract::A procedure was developed to purify dermonecrotic toxin (DNT) from a sonic extract of a serotype D strain of Pasteurella multocida. Sonic extract containing DNT was applied to a DEAE-Sephacel column and eluted by a linear gradient of NaCl. Upon rechromatographing, fractions with dermonecrotic activity for guinea pigs ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nakai T,Sawata A,Tsuji M,Samejima Y,Kume K

    更新日期:1984-11-01 00:00:00

  • Ultrastructural and immunological aspects of the phagocytosis of Trypanosoma brucei by mouse peritoneal macrophages.

    abstract::Trypanosome-activated mouse peritoneal macrophages phagocytized and digested Trypanosoma brucei in vitro and in vivo, but in the absence of specific antiserum and complement the degree of phagocytosis was minimal. Ultrastructurally, the parasites attached to the macrophage by their flagella, and ingestion proceeded fl...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Stevens DR,Moulton JE

    更新日期:1978-03-01 00:00:00

  • Toll-like receptor 2 recognition of the microsporidia Encephalitozoon spp. induces nuclear translocation of NF-kappaB and subsequent inflammatory responses.

    abstract::Microsporidia are obligate intracellular parasites that are ubiquitous in nature and have been recognized as causing an important emerging disease among immunocompromised individuals. Limited knowledge exists about the immune response against these organisms, and virtually nothing is known about the receptors involved...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fischer J,Suire C,Hale-Donze H

    更新日期:2008-10-01 00:00:00

  • Specificity of Mycobacterium tuberculosis antigen 5 determined with mouse monoclonal antibodies.

    abstract::Four mouse monoclonal antibodies have been developed which react with Mycobacterium tuberculosis antigen 5. Each of these monoclonal antibodies has been used to prepare immunoabsorbents, and antigen has been isolated from unheated M. tuberculosis H37Ra culture filtrate by affinity chromatography with these absorbents....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Daniel TM,Gonchoroff NJ,Katzmann JA,Olds GR

    更新日期:1984-07-01 00:00:00

  • Schistosome egg antigens elicit a proinflammatory response by trophoblast cells of the human placenta.

    abstract::Schistosomiasis affects nearly 40 million women of reproductive age. Many of these women are infected while pregnant and lactating. Several studies have demonstrated transplacental trafficking of schistosome antigens; however, little is known regarding how these antigens affect the developing fetus and placenta. To ev...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McDonald EA,Kurtis JD,Acosta L,Gundogan F,Sharma S,Pond-Tor S,Wu HW,Friedman JF

    更新日期:2013-03-01 00:00:00

  • Binding of Haemophilus influenzae to purified mucins from the human respiratory tract.

    abstract::Mucins are high-molecular-weight glycoproteins and major constituents of the mucus layer which covers the airway surface. We have studied the interactions between bacteria, mucins, and epithelial cells from the human respiratory tract. Nontypeable strains of Haemophilus influenzae were found to bind to purified airway...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Davies J,Carlstedt I,Nilsson AK,Håkansson A,Sabharwal H,van Alphen L,van Ham M,Svanborg C

    更新日期:1995-07-01 00:00:00

  • Role of avian pathogenic Escherichia coli virulence factors in bacterial interaction with chicken heterophils and macrophages.

    abstract::Avian pathogenic Escherichia coli (APEC) cause extraintestinal disease in avian species via respiratory tract infection. Virulence factors associated with APEC include type 1 and P fimbriae, curli, aerobactin, lipopolysaccharide (LPS), K1 capsular antigen, temperature-sensitive hemagglutinin (Tsh), and an uncharacteri...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mellata M,Dho-Moulin M,Dozois CM,Curtiss R 3rd,Lehoux B,Fairbrother JM

    更新日期:2003-01-01 00:00:00

  • The Burkholderia cepacia epidemic strain marker is part of a novel genomic island encoding both virulence and metabolism-associated genes in Burkholderia cenocepacia.

    abstract::The Burkholderia cepacia epidemic strain marker (BCESM) is a useful epidemiological marker for virulent B. cenocepacia strains that infect patients with cystic fibrosis. However, there was no evidence that the original marker, identified by random amplified polymorphic DNA fingerprinting, contributed to pathogenicity....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Baldwin A,Sokol PA,Parkhill J,Mahenthiralingam E

    更新日期:2004-03-01 00:00:00

  • Comparison of the contributions of heat-labile enterotoxin and heat-stable enterotoxin b to the virulence of enterotoxigenic Escherichia coli in F4ac receptor-positive young pigs.

    abstract::In swine, the most common and severe enterotoxigenic Escherichia coli (ETEC) infections are caused by strains that express K88 (F4)(+) fimbriae, heat-labile enterotoxin (LT), heat-stable enterotoxin b (STb), and enteroaggregative E. coli heat-stable toxin 1. Previous studies based on a design that involved enterotoxin...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Erume J,Berberov EM,Kachman SD,Scott MA,Zhou Y,Francis DH,Moxley RA

    更新日期:2008-07-01 00:00:00

  • Chronic arthritis induced in rats by cell wall fragments of Eubacterium species from the human intestinal flora.

    abstract::To investigate arthritis-inducing properties of Eubacterium species, which are major residents of the human intestinal flora, cell wall fragments (CWF) of several Eubacterium strains were prepared and tested in an animal model. After a single intraperitoneal injection in the rat, CWF of E. aerofaciens, E. contortum, a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Severijnen AJ,van Kleef R,Hazenberg MP,van de Merwe JP

    更新日期:1990-02-01 00:00:00

  • Opsonophagocytic killing activity of rabbit antibody to Pseudomonas aeruginosa mucoid exopolysaccharide.

    abstract::We used an in vitro opsonophagocytic killing assay to measure the functional activity of antibody directed at the mucoid exopolysaccharide (MEP) antigen expressed by Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Rabbit antibodies raised to purified MEP were able to mediate phagocytic killing i...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ames P,DesJardins D,Pier GB

    更新日期:1985-08-01 00:00:00

  • Human response to Escherichia coli O157:H7 infection: antibodies to secreted virulence factors.

    abstract::Vaccination has been proposed for the prevention of disease due to enterohemorrhagic Escherichia coli (EHEC), but the immune response following human infection, including the choice of potential antigens, has not been well characterized. To study this, sera were obtained from five pediatric patients with acute diarrhe...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Li Y,Frey E,Mackenzie AM,Finlay BB

    更新日期:2000-09-01 00:00:00

  • Identification and quantitation of capsular antigen in capsulated and noncapsulated strains of Haemophilus influenzae type b by crossed-immunoelectrophoresis.

    abstract::Sonicated preparations of capsulated Haemophilus influenzae type b, two of its spontaneous mutants, one containing patches of capsules (class I variant) and the other noncapsulated (class II variant), and a noncapsulated strain of H. influenzae type d were analyzed by crossed-immunoelectrophoresis using unadsorbed ant...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Buckmire FL

    更新日期:1976-06-01 00:00:00

  • CD40 induces anti-Toxoplasma gondii activity in nonhematopoietic cells dependent on autophagy proteins.

    abstract::Toxoplasma gondii infects both hematopoietic and nonhematopoietic cells and can cause cerebral and ocular toxoplasmosis, as a result of either congenital or postnatally acquired infections. Host protection likely acts at both cellular levels to control the parasite. CD40 is a key factor for protection against cerebral...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Van Grol J,Muniz-Feliciano L,Portillo JA,Bonilha VL,Subauste CS

    更新日期:2013-06-01 00:00:00

  • The Genotoxin Colibactin Is a Determinant of Virulence in Escherichia coli K1 Experimental Neonatal Systemic Infection.

    abstract::Escherichia coli strains expressing the K1 capsule are a major cause of sepsis and meningitis in human neonates. The development of these diseases is dependent on the expression of a range of virulence factors, many of which remain uncharacterized. Here, we show that all but 1 of 34 E. coli K1 neonatal isolates carrie...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McCarthy AJ,Martin P,Cloup E,Stabler RA,Oswald E,Taylor PW

    更新日期:2015-09-01 00:00:00

  • Involvement of fractalkine/CX3CL1 expression by dendritic cells in the enhancement of host immunity against Legionella pneumophila.

    abstract::Legionnaires' disease is clinically manifested as severe pneumonia caused by Legionella pneumophila. However, the dendritic cell (DC)-centered immunological framework of the host defense against L. pneumophila has not been fully delineated. For this study, we focused on a potent chemoattractant for lymphocytes, fracta...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kikuchi T,Andarini S,Xin H,Gomi K,Tokue Y,Saijo Y,Honjo T,Watanabe A,Nukiwa T

    更新日期:2005-09-01 00:00:00

  • Bordetella pertussis adenylate cyclase-hemolysin induces interleukin-6 secretion by human tracheal epithelial cells.

    abstract::After interaction with tracheal epithelial cells, Bordetella pertussis induces the secretion of interleukin-6. This secretion is dependent on the expression of adenylate cyclase-hemolysin by the bacterium but not on the expression of other characterized bacterial toxins or adhesins. This finding confirms the important...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bassinet L,Fitting C,Housset B,Cavaillon JM,Guiso N

    更新日期:2004-09-01 00:00:00

  • Polymorphism in the aerobactin-cloacin DF13 receptor genes from an enteroinvasive strain of Escherichia coli and pColV-K30 is associated only with a decrease in cloacin susceptibility.

    abstract::We have cloned chromosomal genes mediating the aerobactin iron transport system from the enteroinvasive strain Escherichia coli 978-77. The physical map of the region spanning the siderophore biosynthesis genes and the upstream portion of the receptor gene in strain 978-77-derived clones was identical to the correspon...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Marolda CL,Valvano MA,Crosa JH

    更新日期:1991-01-01 00:00:00

  • Modulation of the immune response to pneumococcal type 14 capsular polysaccharide-protein conjugates by the adjuvant Quil A depends on the properties of the conjugates.

    abstract::Streptococcus pneumoniae type 14 capsular polysaccharide-bovine serum albumin (S14PS-BSA) conjugates were prepared by water-soluble-carbodiimide-mediated condensation with or without the use of N-hydroxy-sulfosuccinimide. The immunogenicities of the capsular polysaccharide (S14PS) and of the conjugates were studied in...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Verheul AF,Versteeg AA,De Reuver MJ,Jansze M,Snippe H

    更新日期:1989-04-01 00:00:00

  • Naturally acquired immune responses to Plasmodium falciparum sexual stage antigens Pfs48/45 and Pfs230 in an area of seasonal transmission.

    abstract::Acquisition of immunity to Plasmodium falciparum sexual stages is a key determinant for reducing human-mosquito transmission by preventing the fertilization and the development of the parasite in the mosquito midgut. Naturally acquired immunity against sexual stages may therefore form the basis for the development of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ouédraogo AL,Roeffen W,Luty AJ,de Vlas SJ,Nebie I,Ilboudo-Sanogo E,Cuzin-Ouattara N,Teleen K,Tiono AB,Sirima SB,Verhave JP,Bousema T,Sauerwein R

    更新日期:2011-12-01 00:00:00

  • Role of the Agr-like quorum-sensing system in regulating toxin production by Clostridium perfringens type B strains CN1793 and CN1795.

    abstract::Clostridium perfringens type B causes enteritis and enterotoxemia in domestic animals. By definition, these bacteria must produce alpha toxin (CPA), beta toxin (CPB) and epsilon toxin (ETX) although most type B strains also produce perfringolysin O (PFO) and beta2 toxin (CPB2). A recently identified Agr-like quorum-se...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Chen J,McClane BA

    更新日期:2012-09-01 00:00:00