Abstract:
:Trichomonas vaginalis infected with a double-stranded RNA virus undergoes phenotypic variation on the basis of surface versus cytoplasmic expression of the immunogenic protein P270. Examination of batch cultures by flow cytofluorometry with monoclonal antibody (MAb) to P270 yields both fluorescent and nonfluorescent trichomonads. Greater numbers and intensity of fluorescent organisms with surface P270 reactive with MAb were evident in parasites grown in medium depleted of iron. Placement of iron-limited organisms in medium supplemented with iron gave increased numbers of nonfluorescent trichomonads. Purified subpopulations of trichomonads with and without surface P270 obtained by fluorescence-activated cell sorting reverted to nonfluorescent and fluorescent phenotypes when placed in high- and low-iron media, respectively. No similar regulation by iron of P270 was evident among virus-negative T. vaginalis isolates or virus-negative progeny trichomonads derived from virus-infected isolates. Equal amounts of P270 were detectable by MAb on immunoblots of total proteins from identical numbers of parasites grown in low- and high-iron media. Finally, P270 was found to be highly phosphorylated in high-iron parasites. Iron, therefore, plays a role in modulating surface localization of P270 in virus-harboring parasites.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Alderete JFdoi
10.1128/IAI.67.8.4298-4302.1999keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
4298-302issue
8eissn
0019-9567issn
1098-5522journal_volume
67pub_type
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journal_title:Infection and immunity
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doi:10.1128/IAI.59.1.398-406.1991
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journal_title:Infection and immunity
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doi:10.1128/IAI.25.1.366-375.1979
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journal_title:Infection and immunity
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doi:10.1128/IAI.63.2.724-728.1995
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1032-1040.1982
更新日期:1982-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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更新日期:1998-04-01 00:00:00
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更新日期:2004-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1520-1527.1994
更新日期:1994-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3187-3195.1995
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更新日期:1977-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1989-11-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1990-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4740-4743.1991
更新日期:1991-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1984-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1497-1500.1994
更新日期:1994-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1972-12-01 00:00:00