Abstract:
:The mucosal and systemic immune responses after primary and booster immunizations with two attenuated live oral vaccine strains derived from a noninvasive (Vibrio cholerae) and an invasive (Salmonella typhi) enteric pathogen were comparatively evaluated. Vaccination with S. typhi Ty21a elicited antibody-secreting cell (ASC) responses specific for S. typhi O9, 12 lipopolysaccharide (LPS), as well as significant increases in levels of immunoglobulin G (IgG) and IgA antibodies to the same antigen in serum. A strong systemic CD4(+) T-helper type 1 cell-mediated immune (CMI) response was also induced. In contrast to results with Ty21a, no evidence of a CMI response was obtained after primary immunization with V. cholerae CVD 103-HgR in spite of the good immunogenicity of the vaccine. Volunteers who received a single dose of CVD 103-HgR primarily developed an IgM ASC response against whole vaccine cells and purified V. cholerae Inaba LPS, and seroconversion of serum vibriocidal antibodies occurred in four of five subjects. Serum IgG anti-cholera toxin antibody titers were of lower magnitude. For both live vaccines, the volunteers still presented significant local immunity 14 months after primary immunization, as revealed by the elevated baseline antibody titers at the time of the booster immunization and the lower ASC, serum IgG, and vibriocidal antibody responses after the booster immunization. These results suggest that local immunity may interfere with colonization of the gut by both vaccine strains at least up to 14 months after basis immunization. Interestingly, despite a low secondary ASC response, Ty21a was able to boost both humoral (anti-LPS systemic IgG and IgA) and CMI responses. Evidence of a CMI response was also observed for one of three volunteers given a cholera vaccine booster dose. The direct comparison of results with two attenuated live oral vaccine strains in human volunteers clearly showed that the capacity of the vaccine strain to colonize specific body compartments conditions the pattern of vaccine-induced immune responses.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Viret JF,Favre D,Wegmüller B,Herzog C,Que JU,Cryz SJ Jr,Lang ABdoi
10.1128/IAI.67.7.3680-3685.1999keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
3680-5issue
7eissn
0019-9567issn
1098-5522journal_volume
67pub_type
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journal_title:Infection and immunity
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doi:10.1128/IAI.00041-07
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journal_title:Infection and immunity
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doi:10.1128/IAI.14.4.970-975.1976
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2541-2543.1992
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2851-2852.1991
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.168-171.1991
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journal_title:Infection and immunity
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/iai.68.12.6962-6969.2000
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.2.569-580.1976
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.1.195-203.1976
更新日期:1976-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2629-2639.1997
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.7.2861-2867.1997
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2564-2569.1997
更新日期:1997-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00118-19
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2497-2501.1997
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.4.1460-1467.1993
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journal_title:Infection and immunity
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doi:10.1128/IAI.62.8.3576-3579.1994
更新日期:1994-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.356-358.1982
更新日期:1982-01-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.26.2.402-407.1979
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3791-3802.1993
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1182-1186.1975
更新日期:1975-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.199-207.1981
更新日期:1981-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.698-706.1981
更新日期:1981-05-01 00:00:00