Abstract:
:Species-specific antibody epitopes within several major immunoreactive protein orthologs of Ehrlichia species have recently been identified and molecularly characterized. In this study, dominant B-cell epitopes within the acidic (pI 5.35) ankyrin repeat-containing 200-kDa major immunoreactive protein (gp200) of Ehrlichia canis were defined. The E. canis gp200 gene (4,263 bp; 1,421 amino acids) was cloned and expressed as four (N-terminal, 1,107 bp; N-internal, 910 bp; C-internal, 1,000 bp; and C-terminal, 1,280 bp) overlapping recombinant proteins. The N-terminal, C-internal, and C-terminal polypeptides (369, 332, and 426 amino acids, respectively) were strongly recognized by antibody, and the major epitope(s) in these polypeptides was mapped to four polypeptide regions (40 to 70 amino acids). Smaller overlapping recombinant polypeptides (14 to 15 amino acids) spanning these regions identified five strongly immunoreactive species-specific epitopes that exhibited conformational dependence. The majority of the epitopes (four) were located in two strongly acidic (pI 4 to 4.9) domains in the distal N- and C-terminal regions of the protein flanking the centralized ankyrin domain-containing region. The amino acid content of the epitope-containing domains included a high proportion of strongly acidic amino acids (glutamate and aspartate), and these domains appear to have important biophysical properties that influence the antibody response to gp200.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Nethery KA,Doyle CK,Zhang X,McBride JWdoi
10.1128/IAI.00041-07subject
Has Abstractpub_date
2007-10-01 00:00:00pages
4900-8issue
10eissn
0019-9567issn
1098-5522pii
IAI.00041-07journal_volume
75pub_type
杂志文章abstract::Recruitment of neutrophils to lung tissue and airspaces is a hallmark of inflammatory events following inhalation of endotoxins. We studied the role of different lymphocyte subsets in this inflammation, which is assumed to primarily involve the innate immune system. Inhalation of aerosolized Escherichia coli lipopolys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6962-6969.2000
更新日期:2000-12-01 00:00:00
abstract::Salmonella typhimurium SH5014 porins induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin 1 alpha (IL-1 alpha), and IL-6 by human monocytes and of gamma interferon (IFN-gamma) and IL-4 by human lymphocytes. Porins at 1 microgram/ml induce the greatest release of TNF-alpha, IL-1 alpha, and IL-6 by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.155-161.1993
更新日期:1993-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.2.433-437.1987
更新日期:1987-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00153-16
更新日期:2016-09-19 00:00:00
abstract::B-cell-deficient C57B1/6J mice (suppressed from birth with goat anti-mu) and controls (treated from birth with normal goat serum) were infected with Trypanosoma rhodesiense. There was a significant (P less than 0.01) decrease in duration of survival of the mu-suppressed mice compared with that of controls. Whereas bot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.434-438.1977
更新日期:1977-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7099-7108.2003
更新日期:2003-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00849-17
更新日期:2018-02-20 00:00:00
abstract::ExoU is a potent effector protein that causes rapid host cell death upon injection by the type III secretion system of Pseudomonas aeruginosa. The N-terminal half of ExoU contains a patatin-like phospholipase A(2) (PLA(2)) domain that requires the host cell cofactor superoxide dismutase 1 (SOD1) for activation, while ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00223-10
更新日期:2010-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1842-1845.1996
更新日期:1996-05-01 00:00:00
abstract::Encapsulated Staphylococcus aureus strains are more virulent than unencapsulated staphylococci, and this phenomenon has been associated with decreased opsonization of encapsulated bacteria by normal human serum. Peptidoglycan, a major cell wall component of S. aureus, has been shown to promote opsonization of this bac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1159-1163.1979
更新日期:1979-12-01 00:00:00
abstract::Antigen-specific T-cell responses and histopathological changes were studied in mice experimentally inoculated with Borrelia burgdorferi B31. Inbred mice with different H-2 haplotypes and/or different genetic backgrounds were inoculated with B. burgdorferi organisms and tested for antigen-specific T-cell responses in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.41-47.1989
更新日期:1989-01-01 00:00:00
abstract::The immune response of mice to live, heat-killed, or autoclaved Mycobacterium leprae was investigated. After sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. A selected number of these mice were infected with 5 X 10(3) M. leprae, and the re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.79-83.1984
更新日期:1984-01-01 00:00:00
abstract::Studies of mice suggest that pneumococcal proteins, including PspA, pneumolysin, PsaA, and CbpA, are promising vaccine candidates. To determine whether these proteins are good mucosal immunogens in humans, adenoidal lymphocytes from 20 children who had adenoidectomies were isolated and tested by ELISpot for antigen-sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5363-5369.2002
更新日期:2002-10-01 00:00:00
abstract::Previously, we isolated a high-molecular-weight immunogenic polysaccharide (designated PS) from Pseudomonas aeruginosa immunotype 1 (IT-1). The method which we used was modified to permit the isolation of a similar PS from P. aeruginosa IT-2. This antigen was composed primarily of carbohydrate, had a complex monosacch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.461-468.1981
更新日期:1981-11-01 00:00:00
abstract::The ocular damage in young adult mice produced by purified Pseudomonas aeruginosa exotoxin A was microscopically characterized at 1 and 5 h and at 1, 3, 5, 7, 10, 14, and 21 days after toxin A challenge, using light, transmission, and scanning electron microscopic techniques. Similarly to previously described infectio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.1025-1035.1981
更新日期:1981-12-01 00:00:00
abstract::The relationship between the selective abilities of bacteria to adhere and their predilections for colonizing different mammalian hosts was investigated by using bacteria indigenous to the tongue dorsum of humans and rats as models. Streptococcus salivarius and S. sanguis averaged 22.6 and 2.8%, respectively, of the c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.1.238-246.1976
更新日期:1976-01-01 00:00:00
abstract::Toxoplasma gondii is a protozoan parasite that is able to penetrate human monocytes by either passive uptake during phagocytosis or active penetration. It is expected that immunoglobulin G (IgG) opsonization will target the parasite to macrophage Fc gamma receptors for phagocytic processing and subsequent degradation....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4290-4294.1995
更新日期:1995-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3361-3370.2003
更新日期:2003-06-01 00:00:00
abstract::The ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine WEHI 231 cells, splenic B cells, and human RAJI cells was examined. The culture filtrate of Porphyromonas gingivalis, Prevotella loescheii, and Fusobacterium nucleatum, which contains high a percentag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2587-2594.1998
更新日期:1998-06-01 00:00:00
abstract::Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and chronic lung infections in cystic fibrosis patients. Iron is essential for bacterial growth, and P. aeruginosa expresses multiple iron uptake systems, whose role in lung infection deserves further investigation. P. aeruginosa Fe(3+) uptake sy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00098-16
更新日期:2016-07-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.3793-3802.2004
更新日期:2004-07-01 00:00:00
abstract::The adjuvant action of various lipopolysaccharides on immune responses to syngeneic tissue extract in mice was examined. Only lipopolysaccharides possessing the linear mannose homopolysaccharides as O-specific polysaccharides exhibited definite adjuvant action on immune responses to the autoantigens. The intensity of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4953-4956.1992
更新日期:1992-11-01 00:00:00
abstract::The kinetics of Bacillus anthracis toxin production in culture and its lethal activity in rats, mice, and guinea pigs were investigated. Lethal toxin activity was produced in vitro throughout exponential growth at essentially identical rates in both encapsulated virulent and nonencapsulated avirulent strains. The two ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.761-767.1984
更新日期:1984-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1110-1113.1984
更新日期:1984-03-01 00:00:00
abstract::Monoclonal antibodies were raised against a bactericidal protein fraction that was purified from an extract of bovine neutrophil granules and that was previously shown (A. Savoini, R. Marzari, L. Dolzani, D. Serranò, G. Graziosi, R. Gennaro, and D. Romeo, Antimicrob. Agents Chemother. 26:405-407, 1984) to inhibit bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2193-2197.1988
更新日期:1988-08-01 00:00:00
abstract::The present study was performed to examine whether Porphyromonas gingivalis fimbriae induce specifically a protein kinase-mediated phosphorylated protein that is involved in the mechanism of signal transduction. The fimbriae induced a 68-kDa phosphorylated protein (pp68) in a dose-dependent manner in mouse peritoneal ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5242-5246.1994
更新日期:1994-12-01 00:00:00
abstract::Previous experimentation has highlighted a number of difficulties in the development of carrier-based bivalent vaccines (J.-F. Viret and D. Favre, Biologicals 22:361-372, 1994) In an attempt to obviate these carrier strains. Toward this aim, a series of defined rfbInaba deletion (delta rfbInaba) mutants of the cholera...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.576-584.1996
更新日期:1996-02-01 00:00:00
abstract::Patients with chronic mucocutaneous candidiasis (CMC) are selectively unable to clear the yeast Candida, which results in persistent debilitating infections affecting the skin, nails, and mucous membranes. The underlying defect is unknown. Recent animal studies highlighted the importance of type 1 cytokines in protect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5690-5699.2003
更新日期:2003-10-01 00:00:00
abstract::Purified R-28 antigen from an M-protein-poor, R-antigen-rich strain of group A Streptococcus was prepared by sequential treatment of an acid extract of whole cells with ammonium sulfate fractionation and hydroxylapatite (HA) column chromatography. Purified R-28 antigen was eluted only with 0.10 M sodium phosphate, pH ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.901-909.1975
更新日期:1975-10-01 00:00:00
abstract::Vesicular stomatitis virus (Indiana strain) will only grow in T lymphocytes which have been stimulated to undergo cell division. Evidence is presented that a considerable enrichment of the vesicular stomatitis virus-replicating T cells may be accomplished in the mouse spleen by passing the spleen cells over glass wool...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.169-172.1981
更新日期:1981-04-01 00:00:00