Abstract:
:Purified R-28 antigen from an M-protein-poor, R-antigen-rich strain of group A Streptococcus was prepared by sequential treatment of an acid extract of whole cells with ammonium sulfate fractionation and hydroxylapatite (HA) column chromatography. Purified R-28 antigen was eluted only with 0.10 M sodium phosphate, pH 6.7. Findings on quantitative amino acid composition, polyacrylamide gel electrophoresis pattern, and HA column elution pattern were similar but not identical to those previously reported for streptococcal M-proteins. Rabbits immunized with either HA-purified R-28 antigen or heat-killed cells developed two pepsin-sensitive, trypsin-resistant immunodiffusion lines of identity against HA-purified R-28 antigen but failed to form bactericidal antibody. One of these two lines formed a line of identity with R-28 antigen prepared by trypsinization of whole cells. The other line remained undefined, although it appeared not to be either streptococcal group A carbohydrate, M-protein, T-antigen, polyglycerophosphate, E4 antigen, or M-associated protein; by enzymatic criteria it is an R-antigen. Polyacrylamide gel electrophoresis of HA-purified R-28 antigen revealed multiple serologically active charge and size isomers. These findings suggest possible structural similarities between group A streptococcal M-proteins and R-antigens and also indicate that the same purification techniques may be utilized to study these protein antigens if the proper strain of Streptococcus is chosen.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Johnson RHdoi
10.1128/IAI.12.4.901-909.1975keywords:
subject
Has Abstractpub_date
1975-10-01 00:00:00pages
901-9issue
4eissn
0019-9567issn
1098-5522journal_volume
12pub_type
杂志文章abstract::Major research efforts are directed towards the development of a better antimycobacterial vaccine. But progress in the field of tuberculosis vaccine development has been hampered by the lack of human in vitro models to assess vaccine immunogenicity and efficacy. New candidate vaccines will have to be evaluated against...
journal_title:Infection and immunity
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doi:10.1128/IAI.00027-12
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doi:10.1128/IAI.00778-15
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doi:10.1128/IAI.69.7.4654-4656.2001
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abstract::Isolated Bacteroides gingivalis 2561 vesicles aggregated suspensions of Actinomyces viscosus and Actinomyces naeslundii of all taxonomy clusters. Vesicles bound near A. viscosus cell walls and among its surface fibrils. Tritiated vesicles bound slightly better to saliva-coated hydroxyapatite (SHA) than to SHA coated w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1618-1620.1989
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pub_type: 杂志文章
doi:10.1128/iai.71.2.801-813.2003
更新日期:2003-02-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/iai.72.1.229-237.2004
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.680-687.1977
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pub_type: 杂志文章
doi:10.1128/iai.70.8.4124-4131.2002
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2182-2191.2003
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3703-3712.1996
更新日期:1996-09-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.67.7.3566-3570.1999
更新日期:1999-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1520-1527.1994
更新日期:1994-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4629-4636.1993
更新日期:1993-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1813-1819.1992
更新日期:1992-05-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4714-4718.1996
更新日期:1996-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3708-3714.1991
更新日期:1991-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1383-1390.1974
更新日期:1974-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2019-09-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4085-4086.1994
更新日期:1994-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2010-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01128-05
更新日期:2006-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3097-3105.1989
更新日期:1989-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00069-17
更新日期:2017-09-20 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4298-4302.1999
更新日期:1999-08-01 00:00:00