Abstract:
:A water-soluble acylpolygalactosyl (APG) of 34 kDa was obtained from the Klebsiella pneumoniae membrane by alkaline hydrolysis and delipidation. APG comprises a poly(1,3)galactose chain, a core, and a lipid moiety made of a glucosamine disaccharide with two N-linked beta OH-myristates. The monocyte binding sites for APG were investigated by flow cytometry. Biotin-labelled APG (Biot-APG) bound to monocytes at 4 degrees C in the absence of serum, calcium, and magnesium. The binding was dose dependent, saturable, and displaced by unlabelled APG. Neither the polysaccharide chain present in APG-related molecules nor the PPi group or additional ester-linked myristates and palmitates were required for APG binding. The role of CD11b and CD14 was demonstrated by competitive inhibition with monoclonal antibodies and by the uptake of APG by these solubilized proteins. APG was rapidly internalized into monocytes at 37 degrees C while CD14 and CD11b/CD18 molecules were partially down-modulated. Lipopolysaccharides (LPS) from the same K. pneumoniae strain and from Escherichia coli and Salmonella minnesota partially competed for Biot-APG binding in the absence but not in the presence of serum. When altered by alkaline hydrolysis, those LPS became strong competitors for APG binding. It was concluded that alkaline hydrolysis of the K. pneumoniae membrane yielded molecules structurally related to LPS which bind to LPS membrane receptors in the absence of serum.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Hmama Z,Mey A,Normier G,Binz H,Revillard JPdoi
10.1128/IAI.62.5.1520-1527.1994subject
Has Abstractpub_date
1994-05-01 00:00:00pages
1520-7issue
5eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Mice receiving a single injection of cyclophosphamide (150 mg/kg) 1 to 6 days before inoculation with viable Candida albicans showed an increased susceptibility to the challenge accompanied by a reduction in peripheral blood polymorphonuclear leukocytes and lymphocytes as well as in spleen cellularity. Several immunol...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.46-55.1983
更新日期:1983-04-01 00:00:00
abstract::Brewer thioglycolate medium, a bacterial culture medium which is widely used as a nonspecific in vivo macrophage stimulant, was tested for its effect on mouse resistance to bacterial infection. Mice which did or did not receive thioglycolate medium were challenged with Listeria monocytogenes. Injection of thioglycolat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.455-460.1980
更新日期:1980-02-01 00:00:00
abstract::The gram-negative gastric pathogen Helicobacter pylori is equipped with an extraordinarily large set of outer membrane proteins (OMPs), whose role in the infection process is not well understood. The Hop (Helicobacter outer membrane porins) and Hor (Hop-related proteins) groups constitute a large paralogous family con...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00364-09
更新日期:2009-09-01 00:00:00
abstract::The concept of translational research, which aims to facilitate the application of basic scientific discoveries in clinical and community settings, is currently in vogue. While there are powerful forces driving this trend, support for translational research must be accompanied by a robust investment in basic science, ...
journal_title:Infection and immunity
pub_type: 社论,评审
doi:10.1128/IAI.01318-09
更新日期:2010-02-01 00:00:00
abstract::The T-cell-stimulating activity of staphylococcal enterotoxin B (SEB) is an important factor in the pathogenesis of certain staphylococcal diseases. To investigate the immunologically active domains of the SEB molecule, we have produced truncated fragments of recombinant SEB by C-terminal and N-terminal deletions. The...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2445-2452.1993
更新日期:1993-06-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-borne rickettsial organism, causes the disease human monocytic ehrlichiosis. The pathogen also causes disease in several other vertebrates, including dogs and deer. In this study, we assessed two clonally purified E. chaffeensis mutants with insertions within the genes Ech_0379 and Ech_06...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00487-15
更新日期:2015-07-01 00:00:00
abstract::Campylobacter jejuni is a major cause of human inflammatory enteritis. During the course of human disease numerous proinflammatory cytokines are produced. Little is known, however, about the cytokine responses produced during the interaction of this bacterium with the avian host. Campylobacter has been considered a co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2094-2100.2005
更新日期:2005-04-01 00:00:00
abstract::Suppression of the host defense is one of the hallmarks of Yersinia enterocolitica infection. This enteric pathogen resists phagocytosis and interferes with macrophage functions from an extracellular localization (oxidative-burst generation and tumor necrosis factor alpha production). In this study, we investigated th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4813-4821.1997
更新日期:1997-11-01 00:00:00
abstract::A plaque assay system for pathogenic rickettsiae, which utilizes primary chick embryo tissue cultures, is described. It proved to be a highly reproducible measure of infectiousness for Rickettsia rickettsi and R. typhi, which were employed in most studies; as well as for R. canada, R. prowazeki, R. sibirica, R. akari,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.5.715-722.1972
更新日期:1972-05-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium employs two different type III secretion systems (TTSS) encoded within Salmonella pathogenicity islands 1 and 2 (SPI1 and SPI2) for targeting of effector proteins into the cytosol of eukaryotic cells during different stages of the infection cycle. The SPI1 TTSS translocates viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.334-341.2005
更新日期:2005-01-01 00:00:00
abstract::Hematogenous infections caused by Candida krusei have been noted with increasing frequency, particularly in cancer patients receiving prophylaxis with antifungal triazoles. Progress in understanding the pathogenesis of this emerging infection has been limited by the lack of an animal model. We developed a CF1 mouse in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.4.1268-1271.1993
更新日期:1993-04-01 00:00:00
abstract::Mast cell-deficient W/Wv mice and their mast cell-sufficient littermates were given infections of Trichinella spiralis. W/Wv mice were slower than their littermates to expel adult T. spiralis. Repair of the mast cell deficiency of W/Wv mice by bone marrow grafting was accompanied by accelerated expulsion of T. spirali...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.445-447.1983
更新日期:1983-07-01 00:00:00
abstract::Three antigenic determinants were revealed in H3 hemagglutinin of influenza A viruses isolated from 1968 to 1975. One of them was common for all viruses, and two others specified differences between the viruses possessing H3 hemagglutinin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.804-807.1979
更新日期:1979-06-01 00:00:00
abstract::Inoculation of herpes simplex virus on the forehead and/or snout of hairless mice resulted in a significantly lower mortality rate than inoculation of the skin in the lumbosacral area. Latent herpes simplex virus infections were detected in all forehead-inoculated and in 90% of snout-inoculated mice. Phosphonoacetic a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.130-135.1978
更新日期:1978-04-01 00:00:00
abstract::Transposon Tn916 was used to insertionally inactivate a coaggregation-relevant locus of Streptococcus gordonii DL1 (Challis). One mutant (F11) was isolated that lost the ability to coaggregate with the streptococcal partners of DL1 but retained the ability to coaggregate with partners belonging to other genera. A prob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.10.4137-4142.1996
更新日期:1996-10-01 00:00:00
abstract::Previous studies have demonstrated that both Ixodes scapularis saliva and Borrelia burgdorferi antigens modulated lymphokines and monokines in vitro. The studies presented here were designed to delineate the role of I. scapularis and B. burgdorferi in modulation of the host immune response in vivo. Infestation of C3H/...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3100-3106.1997
更新日期:1997-08-01 00:00:00
abstract::Purified beta-toxin from Clostridium perfringens type C was found to be a single polypeptide chain protein with a molecular weight of approximately 30,000. The toxin was heat labile, with 75% of its activity being inactivated by incubation at 50 degrees C for 5 min. Biological activity of the purified toxin was comple...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.678-680.1978
更新日期:1978-08-01 00:00:00
abstract::Oophorectomized, estrogen-treated rats were immunized by the intravaginal or intranasal route with a mannoprotein extract (MP) or secreted aspartyl proteinases (Sap) of Candida albicans, with or without cholera toxin as a mucosal adjuvant. Both routes of immunization were equally effective in (i) inducing anti-MP and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2725-2729.2002
更新日期:2002-05-01 00:00:00
abstract::Pathogenic biofilms have been associated with persistent infections due to their high resistance to antimicrobial agents, while commensal biofilms often fortify the host's immune system. Hence, controlling biofilm formation of both pathogenic bacteria and commensal bacteria is important in bacterium-related diseases. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05580-11
更新日期:2011-12-01 00:00:00
abstract::In order to ascertain if Cryptococcus neoformans components can induce interleukin-6 (IL-6) production, we stimulated human whole blood with purified capsular products. Their potencies in stimulating IL-6 release were mannoproteins > galactoxylomannan = glucuronoxylomannan > alpha(1-3)glucan. IL-6 production was tumor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2454-2456.1997
更新日期:1997-06-01 00:00:00
abstract::A receptor moiety and blood group substance recognized by the O75X adhesin was studied. Well-defined erythrocytes representing different blood group systems and bacterial derivatives carrying plasmid pBJN406 encoding the adhesin were used in a direct hemagglutination assay. We showed that Dr blood group antigen, a com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1057-1060.1988
更新日期:1988-05-01 00:00:00
abstract::The invasion of red blood cells (RBCs) by Plasmodium falciparum is dependent on multiple molecular interactions between erythrocyte receptors and parasite ligands. Invasion studies using culture-adapted parasite strains have indicated significant receptor heterogeneity. It is not known whether this heterogeneity refle...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5886-5891.2004
更新日期:2004-10-01 00:00:00
abstract::The effects of heat and chemical inactivation on the antigenicity and immunogenicity of Vibrio cholerae 1418 in rabbits were studied. V. cholerae 1418 was inactivated with heat and chemical inactivants (phenol or Formalin) alone or in combination. Enzyme-linked immunoassay systems employing whole cells of V. cholerae ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.21-26.1982
更新日期:1982-10-01 00:00:00
abstract::Transgenic tobacco plants stably expressing recombinant FaeG, which is the major subunit and adhesin of K88ad fimbriae, were obtained. Analysis of sera from immunized mice indicates that in mice, the immunogenicity induced by plant-derived FaeG protein is comparable to that generated with traditional approaches. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5436-5439.2003
更新日期:2003-09-01 00:00:00
abstract::At least 10 of 11 demonstrable antigens in unheated culture filtrates of 12 vaccine strains of BCG were shared as determined in a reference antigen-antibody immunoelectrophoresis system. Filtrates from each of the BCG strains gave equivalent skin test reactions in homologously and heterologously sensitized guinea pigs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.5.777-780.1973
更新日期:1973-05-01 00:00:00
abstract::Analysis of the specificity of bactericidal antibodies in normal, convalescent, and postvaccination human sera is important in understanding human immunity to meningococcal infections and can aid in the design of an effective group B vaccine. A collection of human sera, including group C and group B convalescent-phase...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05125-11
更新日期:2011-10-01 00:00:00
abstract::The antigenic cross-reactivity between Treponema pallidum and several pathogenic members of the family Spirochaetaceae was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques. Blots of T. pallidum antigens were incubated with antiserum from rabbits infected or immunize...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.116-121.1984
更新日期:1984-10-01 00:00:00
abstract::Wild-type isolates of Shigella flexneri bind the dye Congo red from solid media, thus producing red (Crb+) colonies. Mutants which fail to bind the dye produce white colonies (Crb-) and are avirulent in a variety of systems. In S. flexneri the ability to bind Congo red correlates with the ability to bind hemin and pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1393-1398.1987
更新日期:1987-06-01 00:00:00
abstract::Microbial survival in a host is usually dependent on the ability of a pathogen to undergo changes that promote escape from host defense mechanisms. The human-pathogenic fungus Cryptococcus neoformans undergoes phenotypic switching in vivo that promotes persistence in tissue. By microarray and real-time PCR analyses, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01079-08
更新日期:2009-01-01 00:00:00
abstract::The Staphylococcus aureus repeat (STAR) element is a sequence identified in two intergenic regions in S. aureus. The element is found in 13 to 21 copies in individual S. aureus strains, and elements in the homologous intergenic location are variable in length. The element sequence consists of several small and unusual...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2344-2348.2000
更新日期:2000-04-01 00:00:00