Abstract:
:Transposon Tn916 was used to insertionally inactivate a coaggregation-relevant locus of Streptococcus gordonii DL1 (Challis). One mutant (F11) was isolated that lost the ability to coaggregate with the streptococcal partners of DL1 but retained the ability to coaggregate with partners belonging to other genera. A probe specific for the region flanking the Tn916 insertion was used to isolate a locus-specific fragment from a chromosomal lambda library. Southern analysis of the resulting phagemids revealed that a 0.5-kb EcoRI fragment hybridized with the F11 probe. Cloning of the 0.5-kb EcoRI fragment into the E. coli-streptococcal insertion vector p(omega) yielded pCW4, which was used to insertionally inactivate the putative coaggregation-relevant gene in DL1. Insertion mutants showed altered coaggregation with streptococci but retained wild-type coaggregation properties with other genera of bacteria. Comparison of immunoblots of cell surface proteins showed a 100-kDa protein in DL1 which was not detected in the Tn916 and pCW4 insertion mutants. These results indicate that the 0.5-kb EcoRI fragment is part of an adhesin-relevant locus that is involved in the production of a 100-kDa protein at the cell surface.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Whittaker CJ,Clemans DL,Kolenbrander PEdoi
10.1128/IAI.64.10.4137-4142.1996subject
Has Abstractpub_date
1996-10-01 00:00:00pages
4137-42issue
10eissn
0019-9567issn
1098-5522journal_volume
64pub_type
杂志文章abstract::The adherence of Trichomonas vaginalis NYH 286 to host cells was evaluated by using monolayer cultures of HeLa and HEp-2 epithelial cells and human fibroblast cell lines. Saturation of sites on HeLa cells was achieved, yielding a maximal T. vaginalis NYH 286-to-cell ratio of two. The ability of radiolabeled NYH 286 to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.701-708.1985
更新日期:1985-12-01 00:00:00
abstract::The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development of optimal therapeutic strategies for invasive infections. CpsY is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00027-12
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abstract::Amastigotes obtained from spleens of mice infected with different strains of Trypanosoma cruzi were examined for their ability to invade macrophages and L929 cells and to initiate infection in mice. Both types of cells were readily invaded by organisms of the strains Y, MR, and Tulahuen. Organisms of the CL strain wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.372-376.1984
更新日期:1984-11-01 00:00:00
abstract::We used an in vitro opsonophagocytic killing assay to measure the functional activity of antibody directed at the mucoid exopolysaccharide (MEP) antigen expressed by Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Rabbit antibodies raised to purified MEP were able to mediate phagocytic killing i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.2.281-285.1985
更新日期:1985-08-01 00:00:00
abstract::The extracellular cysteine protease from Streptococcus pyogenes is a virulence factor that plays a significant role in host-pathogen interaction. Streptococcal protease is expressed as an inactive 40-kDa precursor that is autocatalytically converted into a 28-kDa mature (active) enzyme. Replacement of the single cyste...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4326-4333.1999
更新日期:1999-09-01 00:00:00
abstract::Rabbit endogenous pyrogens occurred in two forms. One was an apparently single protein with a pI of 7.3; the other was a family of proteins with pI values of 4.5 to 5.0. We selected two of the latter, with pI values of 4.6 and 4.72, as representative of the group and compared them with the pI 7.3 pyrogen. Antisera rai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.177-183.1981
更新日期:1981-10-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) is a prominent, food-borne cause of diarrhea, bloody diarrhea, and the hemolytic uremic syndrome in industrialized countries. Most strains of EHEC carry the locus for enterocyte effacement (LEE) pathogenicity island, but a proportion of isolates from patients with severe disea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6307-6319.2003
更新日期:2003-11-01 00:00:00
abstract::The effect of the secondary infection with Toxoplasma gondii on antibody responses to unrelated antigens was examined in mice. A reinfection with 5 X 10(3) organisms did not affect either primary anti-sheep erythrocyte antibody responses, or anti-dinitrophenol antibody responses to dinitrophenylated keyhole limpet hem...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.686-689.1985
更新日期:1985-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1848-1854.1998
更新日期:1998-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.03075-14
更新日期:2015-06-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium employs two different type III secretion systems (TTSS) encoded within Salmonella pathogenicity islands 1 and 2 (SPI1 and SPI2) for targeting of effector proteins into the cytosol of eukaryotic cells during different stages of the infection cycle. The SPI1 TTSS translocates viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.334-341.2005
更新日期:2005-01-01 00:00:00
abstract::The results show that Listeria monocytogenes, Francisella tularensis, and Salmonella typhimurium are facultative intracellular bacteria with a capacity to invade and grow in nonphagocytic cells in vivo. In the liver, all of these pathogens were seen to invade and to multiply extensively in hepatocytes. In all three ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5164-5171.1992
更新日期:1992-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00173-17
更新日期:2017-05-23 00:00:00
abstract::Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from hors...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2776-2782.1999
更新日期:1999-06-01 00:00:00
abstract::An enzyme-linked immunosorbent assay was developed to detect rabbit immunoglobulin G antibodies against purified lipopolysaccharide of the rough Re mutant of Salmonella minnesota. The time necessary for each step was investigated, and this resulted in a test that could be completed in 1 working day. Serial dilutions o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.721-728.1978
更新日期:1978-09-01 00:00:00
abstract::We examined the immunobiological responses to Histoplasma capsulatum in lungs of gamma interferon (IFN-gamma) knockout mice (GKO mice). Naive GKO mice succumbed by day 9 to intranasal challenge with 2.5 x 10(6) yeasts, whereas all wild-type (WT) mice survived for 45 days. Compared to lungs of WT mice, the lungs of acu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2564-2569.1997
更新日期:1997-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.4943-4950.1997
更新日期:1997-12-01 00:00:00
abstract::We have investigated the role of lipoteichoic acid in mediating the adherence of different serotypes of group B streptococci to human adult and neonatal epithelial cells. Pretreatment of neonatal buccal and vaginal epithelial cells with lipoteichoic acid, but not with deacylated lipoteichoic acid, induced a marked inh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.12.3057-3064.1987
更新日期:1987-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2725-2729.2002
更新日期:2002-05-01 00:00:00
abstract::Mycoplasma arthritidis induces an acute to chronic arthritis in rodents. Arthritis induced in mice histologically resembles human rheumatoid arthritis and can be associated with lethal toxicity following systemic injection. The M. arthritidis mitogen (MAM) superantigen has long been implicated as having a role in path...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00667-08
更新日期:2008-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.10.5001-5007.1998
更新日期:1998-10-01 00:00:00
abstract::Escherichia coli O157:H7 causes food and waterborne enteric infections that can result in hemorrhagic colitis and life-threatening hemolytic uremic syndrome. Intimate adherence of the bacteria to intestinal epithelial cells is mediated by intimin, but E. coli O157:H7 also possess several other putative adhesins, inclu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05945-11
更新日期:2012-03-01 00:00:00
abstract::Biofilm formation by the periodontal pathogen Aggregatibacter actinomycetemcomitans is dependent upon autoinducer-2 (AI-2)-mediated quorum sensing. However, the components that link the detection of the AI-2 signal to downstream gene expression have not been determined. One potential regulator is the QseBC two-compone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01376-09
更新日期:2010-07-01 00:00:00
abstract::Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Staphylococcus aureus experimental endocarditis relies on sequential fibrinogen binding (for valve colonization) and fibronectin binding (for endothelial invasion) conferred by peptidoglycan-attached adhesins. Fibronectin-binding protein A (FnBPA) reconciles these two properties--as well as elastin binding--and promot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00405-08
更新日期:2008-08-01 00:00:00
abstract::EspG is a conserved protein encoded by the locus of enterocyte effacement (LEE) of attaching and effacing (A/E) pathogens, including enteropathogenic and enterohemorrhagic Escherichia coli and Citrobacter rodentium. EspG is delivered into infected host cells by a type III secretion system. The role of EspG in virulenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2586-2594.2005
更新日期:2005-05-01 00:00:00
abstract::We have isolated three genes, ARG57, SER57, and LYS1, on the basis of their function in Candida albicans. A C. albicans transformation vector containing the C. albicans URA3 gene, a Candida ARS sequence, and a portion of the Saccharomyces cerevisiae 2 microns circle containing the replication origin was constructed. C...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.876-884.1992
更新日期:1992-03-01 00:00:00
abstract::The symptoms of Lyme disease are caused by inflammation induced by species of the Borrelia burgdorferisensu lato complex. The various presentations of Lyme disease in the population suggest that differences exist in the intensity and regulation of the host response to the spirochete. Previous work has described correl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00160-20
更新日期:2020-10-19 00:00:00
abstract::Tuberculosis remains the leading cause of death among infectious diseases, accounting for more than two million deaths annually. The incidence of the disease is increasing globally, partially because of the resurgence of drug-resistant strains of Mycobacterium tuberculosis. Calixarenes are macrocyclic oligomers, some ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6318-6323.2004
更新日期:2004-11-01 00:00:00
abstract::Alpha-toxin, the major cytolysin of Staphylococcus aureus, preferentially attacks human platelets and cultured monocytes, thereby promoting coagulation and the release of interleukin-1 and tumor necrosis factor. Titers of naturally occurring antibodies in human blood are not high enough to substantially inhibit these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3214-3220.1989
更新日期:1989-10-01 00:00:00