Abstract:
:We have isolated three genes, ARG57, SER57, and LYS1, on the basis of their function in Candida albicans. A C. albicans transformation vector containing the C. albicans URA3 gene, a Candida ARS sequence, and a portion of the Saccharomyces cerevisiae 2 microns circle containing the replication origin was constructed. Clones from genomic libraries in this vector were isolated by direct complementation of the auxotrophies in strain 1006 (arg57 ser57 lys1 ura3 MPA1). Transformants typically contain two to four plasmids in a mixed tandem multimer. A scheme to resolve mixed multimers into monomers in vivo by transformation of S. cerevisiae with Candida transformant DNA selecting Ura+ transformants was devised. Monomeric plasmids were then isolated by transformation of Escherichia coli with the S. cerevisiae transformant DNA. These were retested by transformation of strain 1006 to identify the specific plasmid that complemented the auxotrophy. The chromosomal locations of the genes were determined by hybridization to C. albicans chromosomes separated on contour-clamped homogenous electric field gels. We used these locations to assess the stability of individual C. albicans chromosomes in parasexual genetic analysis. The Lys(+)-complementing clone was shown to be LYS1 by complementation of S. cerevisiae lys1 mutants. These cloned genes help to align the Candida physical and genetic maps and provide additional markers for the transformation system.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Goshorn AK,Grindle SM,Scherer Sdoi
10.1128/IAI.60.3.876-884.1992keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
876-84issue
3eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::Macrophages are among the most sensitive targets of bacterial endotoxin (LPS), responding to minute amounts of LPS by releasing a battery of inflammatory mediators. Transfection of macrophages with secretory leukocyte protease inhibitor (SLPI) renders these cells refractory to LPS stimulation. Here we show that uptake...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4485-4489.1999
更新日期:1999-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01783-05
更新日期:2006-07-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.56.2.370-375.1988
更新日期:1988-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00417-12
更新日期:2012-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4629-4636.1993
更新日期:1993-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.666-673.1982
更新日期:1982-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/iai.71.4.1672-1679.2003
更新日期:2003-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2401-2403.1990
更新日期:1990-07-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2003-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.263-265.1987
更新日期:1987-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2514-2520.1998
更新日期:1998-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05593-11
更新日期:2011-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.5.705-706.1971
更新日期:1971-05-01 00:00:00
abstract::Neospora caninum is a recently identified coccidian parasite which shares many features with, but is clearly distinct from, Toxoplasma gondii. N. caninum tachyzoites infect a wide range of mammalian cells both in vivo and in vitro. The mechanisms by which infection is achieved are largely unknown. Recent evidence has ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.10.4279-4287.1996
更新日期:1996-10-01 00:00:00
abstract::While the pathological events evoked by infection are commonly described, effective host responses to bacteria and their products should primarily be protective. Heat shock protein (Hsp) expression is upregulated by many stimuli and serves to maintain intracellular protein integrity. The ability of the prototypic supe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3187-3194.2004
更新日期:2004-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2758-2765.2003
更新日期:2003-05-01 00:00:00
abstract::In this communication, we examine the specificity of anti-herpes simplex virus (HSV) cytotoxic T lymphocytes (CTL). Serological studies of the two related HSV serotypes (HSV-1 and HSV-2) have revealed both type-specific and cross-reactive antigenic determinants in the viral envelope and on the surface of infected cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.795-803.1981
更新日期:1981-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2715-2721.1994
更新日期:1994-07-01 00:00:00
abstract::Children are more resistant to gingivitis than are adults. To determine possible differences in their periodontal floras, an experimental gingivitis study, identical in design to one reported earlier with young adults, was conducted with four 4- to 6-year-old children. The incidence of sites that developed gingival in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.1-6.1984
更新日期:1984-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.347-353.1977
更新日期:1977-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1895-1899.1987
更新日期:1987-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2602-2607.2000
更新日期:2000-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.2.116-125.1971
更新日期:1971-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.437-441.1985
更新日期:1985-11-01 00:00:00
abstract::Toxin B from Clostridium difficile is cytopathic in vitro for various types of cells, including polymorphonuclear cells, lymphocytes, and monocytes. Since intestine lamina propria is rich in macrophages, we studied the effect of toxin B on human monocytes and on human macrophages generated in vitro by long-term cultur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.1082-1090.1993
更新日期:1993-03-01 00:00:00
abstract::The cytotoxic effects of bacterial lipopolysaccharides (LPS) on mouse leukocytes have been examined in vivo and in vitro. Intraperitoneal injection of LPS into C57BL/6 mice greatly reduced the recovery of mononuclear cells; LPS was cytotoxic for macrophages, but had a mitogenic effect on lymphocytes. Similar effects o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.310-319.1978
更新日期:1978-07-01 00:00:00
abstract::Thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in HeLa 229 cells pretreated with diethylaminoethyl-dextran to determine the relative sensitivity of the two culture systems for detection of the infection. The strains tested were both yolk sac- and HeLa cell-esta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.5.1078-1082.1975
更新日期:1975-11-01 00:00:00
abstract::Hybridoma cell lines which produced monoclonal antibodies against cholera toxin were isolated. These cell lines were detected with an enzyme-linked immunosorbent assay screening procedure with purified cholera toxin or subunit A of cholera toxin. Seven cell lines were characterized with respect to their reactivity wit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.267-272.1982
更新日期:1982-10-01 00:00:00
abstract::Bacteria have evolved strategies to resist killing by antimicrobial peptides (APs), important effectors of innate immunity. The sap (sensitivity to antimicrobial peptides) operon confers resistance to AP-mediated killing of Salmonella. We have recently shown that sapA gene expression is upregulated in the middle ear i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.599-608.2005
更新日期:2005-01-01 00:00:00