Abstract:
:Oral administration of polymyxin to specific-pathogen-free C3H/Law mice which with previously contaminated with gram-negative bacteria resulted in complete suppression of cecal gram-negative bacteria. Suppression of cecal gram-negative bacteria was accompanied by reduction of the cecal endotoxin concentration from 10 to 1 microgram/g of cecal content as measured with a microtechnique for the Limulus amebocyte lysate assay. Endotoxin determination by this assay appeared to be unaffected by the amount of polymyxin present in cecal preparations after oral administration of this antibiotic. In experimentally contaminated specific-pathogen-free mice, the femoral concentration of progenitor cells forming granulocyte-macrophage colonies in vitro (CFU-GM) decreased significantly (P less than 0.001) to 66% of the initial control after 4 days of polymyxin treatment. However, the femoral CFU-GM concentration in germfree mice and splenic CFU-GM concentration in experimentally contaminated specific-pathogen-free and germfree mice was not affected by polymyxin treatment. The kinetic behavior of femoral and splenic CFU-GM in experimentally contaminated specific-pathogen-free and germfree mice was expressed as the in vivo sensitivity to the S-phase-specific cytostatic drug hydroxyurea, i.e., the hydroxyurea kill. Administration of polymyxin to experimentally contaminated specific-pathogen-free mice significantly diminished the hydroxyurea kill of femoral CFU-GM from 29 to 13% (P less than 0.02) and of splenic CFU-GM from 53 to 27% (P less than 0.005). The hydroxyurea kill of femoral CFU-GM in germfree mice was not significantly affected by polymyxin treatment. On basis of these results we conclude that the effect of polymyxin treatment on myelopoiesis is most likely due to elimination of intestinal gram-negative bacteria and may indicate a significant role of intestinal gram-negative bacteria in the regulation of myelopoiesis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Goris H,de Boer F,van der Waaij Ddoi
10.1128/IAI.50.2.437-441.1985subject
Has Abstractpub_date
1985-11-01 00:00:00pages
437-41issue
2eissn
0019-9567issn
1098-5522journal_volume
50pub_type
杂志文章abstract::Intestinal segmented filamentous bacteria (SFB) protect from ameba infection, and protection is transferable with bone marrow dendritic cells (BMDCs). SFB cause an increase in serum amyloid A (SAA), suggesting that SAA might mediate SFB's effects on BMDCs. Here we further explored the role of bone marrow in SFB-mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-16
更新日期:2016-09-19 00:00:00
abstract::Virulent and avirulent clones of Leishmania donovani promastigotes were examined for their acid phosphatase activity. The acid phosphatase activity of whole-cell lysates of virulent clones was 1.5 to 2.0 times higher than that of avirulent clones. Pellet fractions (260,000 x g, 30 min) from sonicated promastigotes of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.11.2856-2860.1988
更新日期:1988-11-01 00:00:00
abstract::As an opportunistic bacterial pathogen, Pseudomonas aeruginosa mainly affects immunocompromised individuals as well as patients with cystic fibrosis. In a previous study, we showed that ExoS of P. aeruginosa, when injected into host cells through a type III secretion apparatus, functions as an effector molecule to tri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3361-3370.2003
更新日期:2003-06-01 00:00:00
abstract::For pathogens to survive in the human oral cavity, they must identify a suitable niche in the complex multispecies biofilm that exists on oral tissues. The periodontal pathogen Porphyromonas gingivalis adheres to Streptococcus gordonii by interacting with a specific region of the streptococcal SspB polypeptide, design...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5736-5741.2001
更新日期:2001-09-01 00:00:00
abstract::Mutant cultures that lacked the ability to produce filamentous hemagglutinin were obtained by UV irradiation of Bordetella pertussis strain 18-323. Various biological activities of these mutants were compared with those of the parent culture. It was found that the leukocytosis-promoting, histamine-sensitizing, and mou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1018-1023.1982
更新日期:1982-03-01 00:00:00
abstract::The hallmarks of pulmonary Mycobacterium tuberculosis infection are lung granulomas. These organized structures are composed of host immune cells whose purpose is to contain or clear infection, creating a complex hub of immune and bacterial cell activity, as well as limiting pathology in the lungs. Yet, given cellular...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00426-18
更新日期:2018-08-22 00:00:00
abstract::Athymic nude mice (nu/nu), heterozygous litter mates (nu/+), and thymic reconstituted homozygous animals (nu/nu TxR) were infected with Trypanosoma rhodesiense. A reduced parasitemia and prolonged survival were observed in the nu/nu animals after infection and cure or after immunization with irradiated organisms. Thes...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.714-720.1978
更新日期:1978-06-01 00:00:00
abstract::Cathepsin G (CatG) is a serine protease found in the azurophilic granules of monocytes that is known to have antimicrobial properties, but its role in Mycobacterium tuberculosis infection is unknown. We found that M. tuberculosis infection of human THP-1 monocytic cells induced the down-regulation of CatG mRNA express...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5712-5721.2004
更新日期:2004-10-01 00:00:00
abstract::Babesiosis has long been recognized as an economically important disease of cattle, but only in the last 30 years has Babesia been recognized as an important pathogen in humans. Invasion of erythrocytes is an integral part of the Babesia life cycle. However, very little information is available on the molecules involv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.649-651.2005
更新日期:2005-01-01 00:00:00
abstract::The mammalian host response to infection includes the production and secretion of antimicrobial peptides from phagocytes and epithelial cells. Protegrins, a group of broadly microbicidal peptides isolated originally from porcine neutrophil lysates, were found to be stored as inactive proforms in porcine neutrophil gra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3611-3617.1998
更新日期:1998-08-01 00:00:00
abstract::Patent filarial infection is associated with the downregulation of parasite-specific immune reactivity. In the present study, the relationship between in vitro parasite antigen-specific humoral and cellular immune responsiveness was investigated in Brugia pahangi-infected jirds and in jirds immunized with soluble anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3052-3057.1988
更新日期:1988-12-01 00:00:00
abstract::Brucella abortus injected into CBA mice replicated primarily in the spleen and liver, reaching a peak bacterial count in both organs about 7 days postinfection. The organism was eliminated from the liver but declined to a chronic phase in the spleen. The infection caused hepatosplenomegaly. An influx of macrophages in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1465-1472.1992
更新日期:1992-04-01 00:00:00
abstract::Attachment of Fusobacterium nucleatum to various oral surfaces is mediated by several adhesins anchored on its outer surface. Monoclonal antibodies (MAbs) were prepared and used to identify the putative galactose-binding adhesin of F. nucleatum PK1594. Four unique MAbs, 8G7, 26B9, 28G11, and 29D4, were isolated on the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5231-5237.1997
更新日期:1997-12-01 00:00:00
abstract::A crude complex containing group C polysaccharide, outer membrane proteins, and lipopolysaccharide (LPS) was isolated from the cell-free culture liquid of Neisseria meningitidis serogroup C, serotype 2a. Group C polysaccharide and LPS were removed from this complex, resulting in an outer membrane complex and a purifie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.369-380.1983
更新日期:1983-04-01 00:00:00
abstract::A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1055-1060.1992
更新日期:1992-03-01 00:00:00
abstract::Exposure of hamster tracheal rings in organ culture to virulent Mycoplasma pneumoniae organisms leads to alterations in macromolecular biosynthesis and metabolic activity of the respiratory epithelial cells. Avirulent organisms derived from the same parent strain do not produce these effects. During the course of infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.704-710.1975
更新日期:1975-04-01 00:00:00
abstract::The Lyme disease spirochete, Borrelia burgdorferi, is introduced into human hosts via tick bites. Among the cell types present in the skin which may initially contact spirochetes are mast cells. Since spirochetes are known to activate a variety of cell types in vitro, we tested whether B. burgdorferi spirochetes could...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1107-1115.1999
更新日期:1999-03-01 00:00:00
abstract::The adherence of Enterococcus faecalis strains to human T24 cells was examined by scanning electron microscopy. Five highly adhesive strains were identified from 30 strains isolated from the urine of patients with urinary tract infections. No efficiently adhesive strains were found among the 30 strains isolated from t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::CD8(+) T lymphocytes often play a primary role in adaptive immunity to cytosolic microbial pathogens. Surprisingly, CD8(+) T cells are not required for protective immunity to the enteric pathogen Shigella flexneri, despite the ability of Shigella to actively secrete proteins into the host cytoplasm, a location from wh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00939-10
更新日期:2011-05-01 00:00:00
abstract::A monoclonal immunoglobulin M antibody, HP15/36, was produced by a hybridoma cell line prepared by fusion of mouse myeloma cell line Sp2/O with spleen cells of mice immunized with Helicobacter pylori D273 (French strain). Immunoelectron microscopy of whole bacteria and ultrathin sections showed that the determinant wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2732-2736.1993
更新日期:1993-06-01 00:00:00
abstract::The type III secretion system (T3SS) plays a crucial role in the pathogenesis of many Gram-negative bacteria, including Edwardsiella tarda, an important fish pathogen. Within the E. tarda T3SS, there are three proteins (EsaB/EsaL/EsaM) that are homologous to proteins present in many other bacteria, including SpiC/SsaL...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00322-17
更新日期:2017-08-18 00:00:00
abstract::CD8 T cells are effective mediators of specific immunity to infection by Listeria monocytogenes, a bacterial pathogen that initially infects macrophages in the spleen and liver and subsequently spreads to hepatocytes and unidentified parenchymal cells in the spleen. To identify the in vivo target cells of L. monocytog...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3632-3640.1996
更新日期:1996-09-01 00:00:00
abstract::The study of how bacteria respond to and obtain divalent metal ions provides insight into the regulation of virulence factors in the host environment. Regulation of metal permease operons in gram-positive bacteria may involve the binding of metal-responsive repressors to palindromic domains in their control regions. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5706-5714.2002
更新日期:2002-10-01 00:00:00
abstract::Stimulated polymorphonuclear leukocytes have been shown by this laboratory to release a reactive-oxygen species (ROS) which is detectable in the supernatant and is capable of oxidizing reduced glutathione and reacting with methionine (Sagone et al., Blood 63:96-104, 1984). This ROS is dependent on H2O2 and heme for it...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.846-849.1984
更新日期:1984-03-01 00:00:00
abstract::Previous experimentation has highlighted a number of difficulties in the development of carrier-based bivalent vaccines (J.-F. Viret and D. Favre, Biologicals 22:361-372, 1994) In an attempt to obviate these carrier strains. Toward this aim, a series of defined rfbInaba deletion (delta rfbInaba) mutants of the cholera...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.576-584.1996
更新日期:1996-02-01 00:00:00
abstract::Bacteria utilize type III secretion systems (T3SS) to deliver effectors directly into host cells. Hence, it is very important to identify the functions of bacterial (T3SS) effectors to understand host-pathogen interactions. Edwardsiella piscicida encodes a functional T3SS effector, EseK, which can be translocated into...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00233-18
更新日期:2018-08-22 00:00:00
abstract::Escherichia coli heat-stable enterotoxin (ST) appears to cause intestinal fluid secretion by activating intestinal particulate guanylate cyclase. Recent studies suggest that chlorpromazine and quinacrine reduce the intestinal secretory response to ST and activation of guanylate cyclase by ST. We have examined the effe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.483-488.1982
更新日期:1982-02-01 00:00:00
abstract::Numerous investigations have shown that 70-kDa heat shock protein (Hsp70) homologs interact tightly with hydrophobic proteins and functionally assist proteins in membranous organelles and environments. One such protein is the Chlamydia trachomatis Hsp70 that is associated with isolated outer membrane complexes of infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.535-543.2002
更新日期:2002-02-01 00:00:00
abstract::Helicobacter pylori maintains colonization in its human host using a limited set of taxis sensors. TlpD is a proposed energy taxis sensor of H. pylori and dominant under environmental conditions of low bacterial energy yield. We studied the impact of H. pylori TlpD on colonization in vivo using a gerbil infection mode...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00750-13
更新日期:2013-10-01 00:00:00
abstract::To explore the molecular basis of the T-cell-mediated immune response to Listeria monocytogenes, we cloned and expressed listerial antigens in Escherichia coli using the lambda-ZAP bacteriophage and Bluescript plasmid vectors. A two-stage screening strategy was implemented to identify T-cell-reactive antigens; the fir...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2792-2803.1990
更新日期:1990-09-01 00:00:00