Partial characterization of an external polysaccharide of Helicobacter pylori by using an immunoglobulin M monoclonal antibody.

Abstract:

:A monoclonal immunoglobulin M antibody, HP15/36, was produced by a hybridoma cell line prepared by fusion of mouse myeloma cell line Sp2/O with spleen cells of mice immunized with Helicobacter pylori D273 (French strain). Immunoelectron microscopy of whole bacteria and ultrathin sections showed that the determinant was located outside the bacterial cell, possibly in the outermost areas. This external reactivity was observed by immunofluorescence and immunoperoxidase assays and was confirmed by immunogold study at the ultrastructural level. The reactive epitope was formol and picric acid resistant and allowed the detection of the bacterium on fixed tissue biopsy specimens. The reactive component was extracted with phenol-water. Immunoblotting with such an antigen exhibited a clearly positive reactivity at a molecular mass between 50 and 120 kDa. This reactivity was suppressed by periodate oxidation, suggesting a carbohydrate epitope. The diagnostic value and significance of this polysaccharide in microbe-host interactions remain to be determined.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Drouet EB,De Montclos HP,Andujar M,Boude M,Grimaud JA,Denoyel GA

doi

10.1128/IAI.61.6.2732-2736.1993

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

2732-6

issue

6

eissn

0019-9567

issn

1098-5522

journal_volume

61

pub_type

杂志文章
  • Influence of preimmunization with tetanus toxoid on immune responses to tetanus toxin fragment C-guest antigen fusions in a Salmonella vaccine carrier.

    abstract::We have previously described a new system for the delivery of recombinant antigens in live Salmonella vaccines as genetic fusions to the C terminus of fragment C of tetanus toxin (TetC) driven by the anaerobically inducible nirB promoter. It has been reported that preimmunization with tetanus toxoid (TT) can suppress ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.7.2564-2569.1995

    authors: Chabalgoity JA,Villareal-Ramos B,Khan CM,Chatfield SN,de Hormaeche RD,Hormaeche CE

    更新日期:1995-07-01 00:00:00

  • Proteomics reveals open reading frames in Mycobacterium tuberculosis H37Rv not predicted by genomics.

    abstract::Genomics revealed the sequence of 3924 genes of the H37Rv strain of Mycobacterium tuberculosis. Proteomics complements genomics in showing which genes are really expressed, and here we show the expression of six genes not predicted by genomics, as proved by two-dimensional electrophoresis and matrix-assisted laser des...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.69.9.5905-5907.2001

    authors: Jungblut PR,Müller EC,Mattow J,Kaufmann SH

    更新日期:2001-09-01 00:00:00

  • Detoxified exoantigens and phosphatidylinositol derivatives inhibit tumor necrosis factor induction by malarial exoantigens.

    abstract::We have previously shown that malaria parasites liberate exoantigens which, through a phospholipid component, stimulate mouse macrophages to secrete tumor necrosis factor (TNF), which are toxic to D-galactosamine-sensitized mice, and which therefore might be involved in pathology. Plasmodium yoelii exoantigens detoxif...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.5.1894-1901.1992

    authors: Bate CA,Taverne J,Playfair JH

    更新日期:1992-05-01 00:00:00

  • A model of sustained gastrointestinal colonization by Candida albicans in healthy adult mice.

    abstract::Three-month-old male Crl:CD1(ICR)BR and C3H/HeJ mice were fed chow containing Candida albicans for 14 days, while similar control mice were fed regular food. Stool cultures were done for all mice before and after administration of the special diet. Stool cultures were repeated 48 h, 1 week, and 1 and 3 months after st...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.6.1514-1517.1990

    authors: Samonis G,Anaissie EJ,Rosenbaum B,Bodey GP

    更新日期:1990-06-01 00:00:00

  • Humoral immune response in acquired immunity to chlamydial genital infection of female guinea pigs.

    abstract::Immunity to reinfection in the genital tract of female guinea pigs with the agent of guinea pig inclusion conjunctivitis was found to be dependent upon an intact humoral immune response. Cell-mediated immunity in the absence of humoral immunity had no apparent role in resistance to challenge infection. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.39.1.463-465.1983

    authors: Rank RG,Barron AL

    更新日期:1983-01-01 00:00:00

  • Energy is required for maximal adherence of Neisseria gonorrhoeae to phagocytic and nonphagocytic cells.

    abstract::The possibility that gonococcal energy might play a role in the interaction of Neisseria gonorrhoeae with both phagocytic and nonphagocytic cells was examined. Respiratory chain inhibitors including KCN and amobarbital resulted in reduction in gonococcal association with human neutrophils. Similar results were seen wi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.3.785-790.1989

    authors: Weber RD,Britigan BE,Svendsen T,Cohen MS

    更新日期:1989-03-01 00:00:00

  • Induction of protective T cells against Listeria monocytogenes in mice by immunization with a listeriolysin O-negative avirulent strain of bacteria and liposome-encapsulated listeriolysin O.

    abstract::Only listeriolysin O (LLO)-producing strains of Listeria monocytogenes generate protective immunity in mice. Based on the findings that endogenous gamma interferon (IFN-gamma) production was induced only by such strains and that purified LLO could induce IFN-gamma from NK cells, we have postulated that LLO may play a ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.2.568-575.1999

    authors: Tanabe Y,Xiong H,Nomura T,Arakawa M,Mitsuyama M

    更新日期:1999-02-01 00:00:00

  • Toll-like receptor 2 activation by Chlamydia trachomatis is plasmid dependent, and plasmid-responsive chromosomal loci are coordinately regulated in response to glucose limitation by C. trachomatis but not by C. muridarum.

    abstract::We previously demonstrated that plasmid-deficient Chlamydia muridarum retains the ability to infect the murine genital tract but does not elicit oviduct pathology because it fails to activate Toll-like receptor 2 (TLR2). We derived a plasmid-cured derivative of the human genital isolate Chlamydia trachomatis D/UW-3/Cx...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01118-10

    authors: O'Connell CM,AbdelRahman YM,Green E,Darville HK,Saira K,Smith B,Darville T,Scurlock AM,Meyer CR,Belland RJ

    更新日期:2011-03-01 00:00:00

  • Heterophil antigen in bovine sera detectable by immune adherence hemagglutination with infectious mononucleosis sera.

    abstract::Bovine sera for tissue culture use were shown to contain heterophil antigen of the Paul-Bunnell-Davidsohn type by immune adherence hemagglutination tests with sera from patients with infectious mononucleosis. The antibody titers observed were comparable to those determined by two other methods; i.e., the immune adhere...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.19.3.923-927.1978

    authors: Lennette ET,Henle G,Henle W,Horwitz CA

    更新日期:1978-03-01 00:00:00

  • Genome-wide identification of Francisella tularensis virulence determinants.

    abstract::Francisella tularensis is a gram-negative pathogen that causes life-threatening infections in humans and has potential for use as a biological weapon. The genetic basis of the F. tularensis virulence is poorly understood. This study screened a total of 3,936 transposon mutants of the live vaccine strain for infection ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01865-06

    authors: Su J,Yang J,Zhao D,Kawula TH,Banas JA,Zhang JR

    更新日期:2007-06-01 00:00:00

  • Response of mice to injection of ribosomal fraction from group B Neisseria meningitidis.

    abstract::Ribosomes of strain NOR-7 of group B Neisseria meningitidis were isolated by a procedure that included treatment of the cells with sodium dodecyl sulfate, disruption in a French pressure cell, and differential centrifugation. These preparations consisted of 66% ribonucleic acid and 24% protein and sedimented as a sing...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.6.3.355-363.1972

    authors: Thomas DW,Weiss E

    更新日期:1972-09-01 00:00:00

  • Elevated Levels of Interleukin-27 in Early Life Compromise Protective Immunity in a Mouse Model of Gram-Negative Neonatal Sepsis.

    abstract::Neonates are at increased risk for bacterial sepsis. We established that the immune-suppressive cytokine interleukin-27 (IL-27) is elevated in neonatal mice. Similarly, human cord blood-derived macrophages express IL-27 genes and secrete more cytokine than macrophages from adults. In the present work, we hypothesized ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00828-19

    authors: Seman BG,Vance JK,Rawson TW,Witt MR,Huckaby AB,Povroznik JM,Bradford SD,Barbier M,Robinson CM

    更新日期:2020-02-20 00:00:00

  • Association of Escherichia coli with the small intestinal epithelium. I. Comparison of enteropathogenic and nonenteropathogenic porcine strains in pigs.

    abstract::Two enteropathogenic strains of Escherichia coli (EEC) differed from a nonenteropathogenic strain of E. coli (NEEC) in their association with porcine small intestinal epithelium. The EEC characteristically were found along villi from tip to base and contiguous to the brush border. They were not in crypts. In contrast,...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.4.595-605.1972

    authors: Bertschinger HU,Moon HW,Whipp SC

    更新日期:1972-04-01 00:00:00

  • Recognition of immunoglobulin A1 by oral actinomyces and streptococcal lectins.

    abstract::Actinomyces naeslundii and Streptococcus gordonii, oral bacteria that possess Gal/GalNAc- and sialic acid-reactive lectins, respectively, were adherent to immobilized secretory immunoglobulin A (IgA) and two IgA1 myeloma proteins but not to two IgA2 myeloma proteins. Apparently, O-linked oligosaccharides at the hinge ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.12.5421-5424.1996

    authors: Ruhl S,Sandberg AL,Cole MF,Cisar JO

    更新日期:1996-12-01 00:00:00

  • Combination Immunotherapy with Passive Antibody and Sulfasalazine Accelerates Fungal Clearance and Promotes the Resolution of Pneumocystis-Associated Immunopathogenesis.

    abstract::The pulmonary immune response protects healthy individuals against Pneumocystis pneumonia (PcP). However, the immune response also drives immunopathogenesis in patients who develop severe PcP, and it is generally accepted that optimal treatment requires combination strategies that promote fungal killing and also provi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00640-19

    authors: Hoy Z,Wright TW,Elliott M,Malone J,Bhagwat S,Wang J,Gigliotti F

    更新日期:2020-01-22 00:00:00

  • Identification of the active precipitin components in a purified preparation of the A antigen of Blastomyces dermatitidis.

    abstract::A purified A-antigen preparation of Blastomyces dermatitidis was determined to be composed of five major glycoprotein bands, visible with Coomassie blue and periodic acid-Schiff staining of polyacrylamide gels. At least 20 additional protein bands were detected by using a silver stain, which was 100 times more sensiti...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.33.1.171-177.1981

    authors: Young KD,Larsh HW

    更新日期:1981-07-01 00:00:00

  • Susceptibility of epithelial cells to Pseudomonas aeruginosa invasion and cytotoxicity is upregulated by hepatocyte growth factor.

    abstract::Normal cell polarity protects epithelial cells against Pseudomonas aeruginosa invasion and cytotoxicity. Using epithelial cell clones with selective defects in sorting of membrane constituents, and using hepatocyte growth factor pretreatment, we found that polarized susceptibility to P. aeruginosa can be altered witho...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.7.3443-3446.1998

    authors: Fleiszig SM,Vallas V,Jun CH,Mok L,Balkovetz DF,Roth MG,Mostov KE

    更新日期:1998-07-01 00:00:00

  • Characterization of a methyl-accepting chemotaxis protein gene, dmcA, from the oral spirochete Treponema denticola.

    abstract::A gene, dmcA, expressing a methyl-accepting chemotaxis protein (MCP) from the oral spirochete Treponema denticola has been characterized. The gene was initially identified as an open reading frame immediately upstream from the previously characterized prtB protease gene of strain ATCC 35405. Nucleotide sequencing of t...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.10.4011-4016.1997

    authors: Kataoka M,Li H,Arakawa S,Kuramitsu H

    更新日期:1997-10-01 00:00:00

  • Construction and characterization of a Vi-positive variant of the Salmonella typhi live oral vaccine strain Ty21a.

    abstract::The viaB locus coding for the Vi antigen of Salmonella typhi Ty2 was cloned on a 40.6-kilobase fragment into the cosmid vector pHC79. The live, oral, attenuated Vi-negative S. typhi Ty21a vaccine strain was transformed with the recombinant cosmid encoding the viaB locus. Homologous recombination of the viaB locus into...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.12.3863-3868.1989

    authors: Cryz SJ Jr,Fürer E,Baron LS,Noon KF,Rubin FA,Kopecko DJ

    更新日期:1989-12-01 00:00:00

  • Inhibition of endotoxin-induced activation of human monocytes by human lipoproteins.

    abstract::Toxicity of lipopolysaccharide (LPS) (endotoxin) is, to a large extent, mediated by the activation of monocytes/macrophages and subsequent release of monokines, such as interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha). It is known that LPS binds readily to serum lipoproteins and that LPS-lipoprotein co...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.7.2237-2245.1989

    authors: Flegel WA,Wölpl A,Männel DN,Northoff H

    更新日期:1989-07-01 00:00:00

  • Antigenic and biological characteristics of mutant strains of Cryptococcus neoformans lacking capsular O acetylation or xylosyl side chains.

    abstract::Cryptococcus neoformans is surrounded by an antiphagocytic polysaccharide capsule whose primary constituent is glucuronoxylomannan (GXM). Three prominent structural features of GXM are single xylosyl and glucuronosyl side chains and O acetylation of the mannose backbone. Isogenic pairs of O-acetyl-positive and O-acety...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.5.2868-2875.2003

    authors: Kozel TR,Levitz SM,Dromer F,Gates MA,Thorkildson P,Janbon G

    更新日期:2003-05-01 00:00:00

  • Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.

    abstract::Performing patch-clamp experiments on human macrophages, we show that the K(+) channel MaxiK is activated by lipopolysaccharide, peptidoglycan, and interleukin-1. Cytokine production initiated by several Toll-like receptor (TLR) ligands and by interleukin-1 is inhibited by MaxiK blockade. This provides evidence for fu...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01783-05

    authors: Scheel O,Papavlassopoulos M,Blunck R,Gebert A,Hartung T,Zähringer U,Seydel U,Schromm AB

    更新日期:2006-07-01 00:00:00

  • Altered Toll-like receptor 9 signaling in Mycobacterium avium subsp. paratuberculosis-infected bovine monocytes reveals potential therapeutic targets.

    abstract::Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease in cattle. The complex, multifaceted interaction of M. avium subsp. paratuberculosis with its host includes dampening the ability of infected cells to respond to stimuli that promote M. avium subsp. paratuberculosis clearance. By dis...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00785-12

    authors: Arsenault RJ,Li Y,Maattanen P,Scruten E,Doig K,Potter A,Griebel P,Kusalik A,Napper S

    更新日期:2013-01-01 00:00:00

  • Micronemal transport of Plasmodium ookinete chitinases to the electron-dense area of the apical complex for extracellular secretion.

    abstract::Plasmodium ookinetes secrete chitinases to penetrate the acellular, chitin-containing peritrophic matrix of the mosquito midgut en route to invasion of the epithelium. Chitinases are potentially targets that can be used to block malaria transmission. We demonstrate here that chitinases of Plasmodium falciparum and P. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.11.6461-6465.2000

    authors: Langer RC,Hayward RE,Tsuboi T,Tachibana M,Torii M,Vinetz JM

    更新日期:2000-11-01 00:00:00

  • Clindamycin-induced enterocolitis in hamsters as a model of pseudomembranous colitis in patients.

    abstract::Stools from a patient with antibiotic-associated colitis and cecal contents from a hamster with clindamycin-induced enterocolitis were compared in a cytotoxicity assay to determine common properties. Both specimens produced actinomorphic changes in human amnion cells at 10(-7) dilutions. The toxin was acid labile, hea...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.20.2.526-529.1978

    authors: Chang TW,Bartlett JG,Gorbach SL,Onderdonk AB

    更新日期:1978-05-01 00:00:00

  • Contribution of cloned virulence factors from uropathogenic Escherichia coli strains to nephropathogenicity in an experimental rat pyelonephritis model.

    abstract::Escherichia coli 536 (O6:K15:H31), which was isolated from a case of urinary tract infection, determines high nephropathogenicity in a rat pyelonephritis system as measured by renal bacterial counts 7 days after infection. The loss of S fimbrial adhesin formation (Sfa-) (mannose-resistant hemagglutination [Mrh-] and f...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.54.3.761-767.1986

    authors: Marre R,Hacker J,Henkel W,Goebel W

    更新日期:1986-12-01 00:00:00

  • An anti-CD3 monoclonal antibody protects mice against a lethal infection with Listeria monocytogenes through induction of endogenous cytokines.

    abstract::Mice were protected against a lethal infection with Listeria monocytogenes when treated with low doses of an anti-CD3 monoclonal antibody (MAb). Injection of anti-CD3 MAb induced rapid production of endogenous tumor necrosis factor (TNF) in the spleens and endogenous gamma interferon (IFN-gamma) in the bloodstreams an...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.7.2786-2792.1993

    authors: Nakane A,Okamoto M,Asano M,Kohanawa M,Minagawa T

    更新日期:1993-07-01 00:00:00

  • Heat-labile enterotoxin can be released from Escherichia coli cells by host intestinal factors.

    abstract::The heat-labile enterotoxin (LT) of Escherichia coli is localized in the periplasm of the bacterial cell. Growth of an LT-producing strain of E. coli in the presence of bile salts, in concentrations within the range found in the human small intestine, caused leakage of LT into the culture medium. This leakage could be...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.1.168-171.1991

    authors: Hunt PD,Hardy SJ

    更新日期:1991-01-01 00:00:00

  • Activity of Lipid Extracts from a Group A Streptococcus against Ehrlich Ascites Carcinoma in Mice.

    abstract::Lipid extracts derived from a group A Streptococcus, when preincubated with tumor cells before inoculation, were shown to prevent completely the development of Ehrlich ascites tumor in mice. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.3.5.705-706.1971

    authors: Kigoshi S

    更新日期:1971-05-01 00:00:00

  • Synthesis, characterization, and clinical evaluation of conjugate vaccines composed of the O-specific polysaccharides of Shigella dysenteriae type 1, Shigella flexneri type 2a, and Shigella sonnei (Plesiomonas shigelloides) bound to bacterial toxoids.

    abstract::The theoretic basis for developing conjugate vaccines, to induce immunoglobulin G (IgG) lipopolysaccharide (LPS) antibodies for the prevention of shigellosis, has been described (J. B. Robbins, C.-Y. Chu, and R. Schneerson, Clin. Infect. Dis. 15:346-361, 1992). The O-specific polysaccharides (O-SPs) of Shigella dysent...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.9.3678-3687.1993

    authors: Taylor DN,Trofa AC,Sadoff J,Chu C,Bryla D,Shiloach J,Cohen D,Ashkenazi S,Lerman Y,Egan W

    更新日期:1993-09-01 00:00:00