Abstract:
:Numerous investigations have shown that 70-kDa heat shock protein (Hsp70) homologs interact tightly with hydrophobic proteins and functionally assist proteins in membranous organelles and environments. One such protein is the Chlamydia trachomatis Hsp70 that is associated with isolated outer membrane complexes of infectious elementary bodies (EB). Previous observations have indicated that chlamydial Hsp70 plays a role in EB attachment to, or entry into, endometrial epithelial cells. In this study, immunofluorescence microscopy and transmission electron microscopy observations showed that chlamydial Hsp70 is not a surface-displayed ligand on purified EB. However, brief exposure of EB to the thiol reducing agent dithiothreitol (DTT) led to surface accessibility of the Hsp70 substrate-binding domain. Reduction of the highly disulfide-cross-linked EB outer membrane proteins with DTT resulted in a decrease in EB attachment and infectivity. Interestingly, exposure of EB to the membrane-impermeable thiol-alkylating reagent 5,5'-dithiobis(2-nitrobenzoic acid) enhanced attachment but compromised infectivity, suggesting that EB outer membrane proteins must be reduced for entry and productive infection. Together, our data suggest that (i) the structural integrity of the EB outer membrane, maintained by protein disulfide bonds, is important during the initial stages of attachment; (ii) reduction occurs within the localized microenvironment of host cell surfaces once intimate contact is established between EB and host cells; and (iii) subsequent conformational changes in EB ultrastructure allow productive infection in host cells. The accessibility of the Hsp70 substrate-binding domain may support the hypothesis that this protein plays a role in events following the initial stage of attachment instead of serving as a primary, surface-displayed adhesin.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Raulston JE,Davis CH,Paul TR,Hobbs JD,Wyrick PBdoi
10.1128/iai.70.2.535-543.2002keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
535-43issue
2eissn
0019-9567issn
1098-5522journal_volume
70pub_type
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journal_title:Infection and immunity
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/IAI.26.1.90-98.1979
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journal_title:Infection and immunity
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更新日期:1998-08-01 00:00:00
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更新日期:1982-01-01 00:00:00
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更新日期:1974-10-01 00:00:00