Abstract:
:Salmonella species utilize type III secretion systems (T3SSs) to translocate effectors into the cytosol of mammalian host cells, subverting cell signaling and facilitating the onset of gastroenteritis. In this study, we compared a draft genome assembly of Salmonella enterica subsp. salamae strain 3588/07 against the genomes of S. enterica subsp. enterica serovar Typhimurium strain LT2 and Salmonella bongori strain 12419. S. enterica subsp. salamae encodes the Salmonella pathogenicity island 1 (SPI-1), SPI-2, and the locus of enterocyte effacement (LEE) T3SSs. Though several key S Typhimurium effector genes are missing (e.g., avrA, sopB, and sseL), S. enterica subsp. salamae invades HeLa cells and contains homologues of S. bongori sboK and sboC, which we named seoC SboC and SeoC are homologues of EspJ from enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), which inhibit Src kinase-dependent phagocytosis by ADP-ribosylation. By screening 73 clinical and environmental Salmonella isolates, we identified EspJ homologues in S. bongori, S. enterica subsp. salamae, and Salmonella enterica subsp. arizonae The β-lactamase TEM-1 reporter system showed that SeoC is translocated by the SPI-1 T3SS. All the Salmonella SeoC/SboC homologues ADP-ribosylate Src E310 in vitro Ectopic expression of SeoC/SboC inhibited phagocytosis of IgG-opsonized beads into Cos-7 cells stably expressing green fluorescent protein (GFP)-FcγRIIa. Concurrently, S. enterica subsp. salamae infection of J774.A1 macrophages inhibited phagocytosis of beads, in a seoC-dependent manner. These results show that S. bongori, S. enterica subsp. salamae, and S. enterica subsp. arizonae share features of the infection strategy of extracellular pathogens EPEC and EHEC and shed light on the complexities of the T3SS effector repertoires of Enterobacteriaceae.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Pollard DJ,Young JC,Covarelli V,Herrera-León S,Connor TR,Fookes M,Walker D,Echeita A,Thomson NR,Berger CN,Frankel Gdoi
10.1128/IAI.00704-16subject
Has Abstractpub_date
2016-11-18 00:00:00pages
3618-3628issue
12eissn
0019-9567issn
1098-5522pii
IAI.00704-16journal_volume
84pub_type
杂志文章abstract::In assessing the host cell range of bovine parvoviruses, these viruses were found to replicate optimally in actively dividing bovine fetal lung and spleen cells. Other primary bovine fetal cells supported growth to a lesser extent, but bovine line cells and line cells of other animal species tested did not. Minimal in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.3.398-402.1973
更新日期:1973-03-01 00:00:00
abstract::Agglutinogen 1 of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen 1 migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.243-248.1971
更新日期:1971-02-01 00:00:00
abstract::Helicobacter mustelae is a urease-rich bacterium associated with gastritis in ferrets. The ureases of H. mustelae and Helicobacter pylori, a bacterium implicated in human gastritis, share many characteristics. Helicobacter sp. ureases appear to be unique among bacterial enzymes in exhibiting submillimolar Km values an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3343-3345.1991
更新日期:1991-09-01 00:00:00
abstract::Our previous work suggests that one mechanism through which connective tissue breakdown might occur in periodontal diseases is the production of metalloproteinases, including collagenase, by gingival fibroblasts. In this study we investigated whether highly purified preparations of lipopolysaccharide (LPS) and lipotei...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2148-2154.1987
更新日期:1987-09-01 00:00:00
abstract::Recent preclinical and epidemiologic studies have suggested that certain Mycobacterium tuberculosis genotypes (in particular, Beijing lineage strains) may be resistant to Mycobacterium bovis BCG vaccine-induced antituberculosis protective immunity. To investigate the strain specificity of BCG-induced protective respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00019-08
更新日期:2008-11-01 00:00:00
abstract::Female mice infected at birth with 10(7) 50% tissue culture infective doses of polyoma virus were mated when at least 6 weeks old. Polyoma was not detected in any tissues of 27 female mice before mating except for trace amounts in the kidneys of 2 mice, but late in gestation polyoma virus could be found in the kidneys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.998-1002.1979
更新日期:1979-09-01 00:00:00
abstract::Depressed Th1 responses are a prominent feature of human tuberculosis, but an enhanced Th2 response has not been detected in peripheral blood T cells stimulated in vitro with Mycobacterium tuberculosis. In disease due to Mycobacterium leprae, Th2 cells predominate in tissue lesions of patients with extensive disease b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1351-1356.1996
更新日期:1996-04-01 00:00:00
abstract::Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis deliver different Yop (Yersinia outer proteins) effector proteins into mammalian cells by a type III secretion mechanism. Recently, it was shown that Yersinia producing YopT leads to disruption of the actin cytoskeleton of HeLa cells (M. Iriarte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7535-7543.2001
更新日期:2001-12-01 00:00:00
abstract::gamma delta T cells have variously been implicated in the protection against, and the pathogenesis of, malaria, but few studies have examined the gamma delta T-cell response to malaria in African children, who suffer the large majority of malaria-associated morbidity and mortality. This is unfortunate, since available...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3190-3196.2001
更新日期:2001-05-01 00:00:00
abstract::Depletion of dendritic cells (DC) in secondary lymphoid organs is a hallmark of sepsis-induced immune dysfunction. In this setting, we investigated if Toll-like receptor (TLR)-dependent signaling might modulate the maturation process and the survival of DC. Using a model of sublethal polymicrobial sepsis induced by ce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00238-09
更新日期:2009-12-01 00:00:00
abstract::Specific proteolytic destruction of the human chemotaxin, C5a, is a property of group A and B streptococcal pathogens. Here we show that virulent group G streptococci from human sources also express C5a peptidase activity. The enzyme responsible for this activity is approximately the same size as and is antigenically ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2305-2310.1991
更新日期:1991-07-01 00:00:00
abstract::Merozoite surface protein 2 (MSP2) is an abundant glycosylphosphatidylinositol (GPI)-anchored protein of Plasmodium falciparum, which is a potential component of a malaria vaccine. As all forms of MSP2 can be categorized into two allelic families, a vaccine containing two representative forms of MSP2 may overcome the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00665-12
更新日期:2012-12-01 00:00:00
abstract::Factors in the queous humor capable of increasing the random migration and chemotaxis of monocytes were demonstrated during experimental uveitis in the rabbit. Injection of endotoxin-free human serum albumin into the vitreous produced a severe inflammation detectable after 7 days and maximal between 12 to 21 days. Aqu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.4.1110-1116.1976
更新日期:1976-04-01 00:00:00
abstract::A group of insertion mutants was used to define the genes of plasmid pDC5 required for the expression of mannose-resistant fimbriae. Minicell experiments identified four polypeptides (71,000, 45,000, 27,000, and 17,000 daltons) concerned with fimbrial production, the smallest of these being the fimbrial subunit. The a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.900-906.1983
更新日期:1983-12-01 00:00:00
abstract::Alveolar macrophages are the effector cells largely responsible for clearance of Pneumocystis carinii from the lungs. Binding of organisms to beta-glucan and mannose receptors has been shown to stimulate phagocytosis of the organisms. To further define the mechanisms used by alveolar macrophages for clearance of P. ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00393-07
更新日期:2007-08-01 00:00:00
abstract::To study the immunological activity of proteins secreted by Mycobacterium tuberculosis, we carried out comparative studies in guinea pigs infected intravenously with 2.5 x 10(3) CFU of this organism or with 2.5 x 10(4) CFU of Mycobacterium bovis BCG. Groups of infected guinea pigs were skin tested with fractions of se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.804-810.1995
更新日期:1995-03-01 00:00:00
abstract::Streptococcus pneumoniae is a major pathogen in humans. The pathogenicity of this organism is related to its many virulence factors, the most important of which is the thick pneumococcal capsule that minimizes phagocytosis. Another virulence-associated trait is the tendency of this bacterium to undergo autolysis in st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00290-09
更新日期:2009-09-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHI) has four loci, lic-1 to lic-3 and lgtC, that generate phase-variable lipooligosaccharide (LOS) structures. lic-1, which is required for the expression of phosphorylcholine (ChoP), is the best characterized and is associated with an enhanced ability of H. influenzae to persist ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.8.4593-4597.2000
更新日期:2000-08-01 00:00:00
abstract::We report the cloning and expression of Ac-GST-1, a novel glutathione S-transferase from the adult hookworm Ancylostoma caninum, and its possible role in parasite blood feeding and as a vaccine target. The predicted Ac-GST-1 open reading frame contains 207 amino acids (mass, 24 kDa) and exhibited up to 65% amino acid ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6903-6911.2005
更新日期:2005-10-01 00:00:00
abstract::Cattle are an important reservoir of Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains, foodborne pathogens that cause hemorrhagic colitis and hemolytic uremic syndrome in humans. EHEC O157:H7 strains are not pathogenic in calves >3 weeks old. Our objective was to determine if EHEC O157:H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1842-1848.1997
更新日期:1997-05-01 00:00:00
abstract::Streptococcus pyogenes secretes a specific immunoglobulin G (IgG)-protease, SpeB, as well as the IgG glycan-hydrolyzing enzyme EndoS. Here we show that SpeB also degrades IgA, IgM, IgD, and IgE. We also show that EndoS only hydrolyzes the glycan moiety on native but not denatured IgG. Thus, SpeB has a broad immunoglob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.7187-7189.2001
更新日期:2001-11-01 00:00:00
abstract::Yersinia enterocolitica serotype 9 contained an antigenic component giving a reaction of total identity with Brucella native hapten and polysaccharide B. This component was present in a phenol-water extract (fraction 5; M. Redfearn, Ph.D. Thesis, University of Wisconsin, Madison, 1960) along with the smooth lipopolysa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.778-780.1982
更新日期:1982-11-01 00:00:00
abstract::Previous studies have shown that tumor necrosis factor alpha (TNF-alpha) plays a pathophysiologic role in sepsis induced in rat pups by group B streptococci (GBS). In this model, TNF-alpha is also partially responsible for the induction of interleukin-6 (IL-6). The present study was undertaken to investigate the role ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4997-5002.1994
更新日期:1994-11-01 00:00:00
abstract::N-Acetyl-muramyl-L-alanyl-D-isoglutamine (MDP), a structurally defined fragment of bacterial peptidoglycan, stimulated significant release of previously incorporated 45Ca from fetal rat bones in tissue culture over the concentration range of 0.1 to 10.0 micrograms/ml. MDP-Stimulated bone resorption was not inhibited b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.133-137.1982
更新日期:1982-01-01 00:00:00
abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2262-2272.2005
更新日期:2005-04-01 00:00:00
abstract::The toxicity of Chlamydia trachomatis was studied with mouse peritoneal macrophage culture. Inoculation of 30 inclusion-forming units of trachoma B/TW-5/OT organisms and 250 inclusion-forming units of lymphogranuloma venereum L2/434/Bu organisms per cell caused immediated toxicity, with the killing of 40 to 90% of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.613-618.1978
更新日期:1978-06-01 00:00:00
abstract::Formation of compact colonies of Staphylococcus aureus in serum-soft agar is mainly a result of a reaction between protein A and the Fc-part of immunoglobulin G and not a clumping factor-fibrinogen reaction. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.583-586.1972
更新日期:1972-10-01 00:00:00
abstract::Although certain helminth infections preferentially induce type 2 T-cell responses, the immunological mechanisms responsible for type 2 T-cell polarization remain unclear. In the present study, we investigated the effects of excretory-secretory (ES) antigen from the nematode Nippostrongylus brasiliensis on cytokine pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.11.6233-6239.2000
更新日期:2000-11-01 00:00:00
abstract::Burkholderia pseudomallei is a CDC tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common, and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02785-14
更新日期:2015-05-01 00:00:00
abstract::Inbred mouse strains differ in their susceptibility to infection with the facultative intracellular bacterium Listeria monocytogenes, largely due to delayed or deficient innate immune responses. Previous antibody depletion studies suggested that neutrophils (polymorphonuclear leukocytes [PMN]) were particularly import...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00085-18
更新日期:2018-03-22 00:00:00