Abstract:
:Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread during neutropenia. Mice received 2 mg of streptomycin/ml of drinking water and 1,500 U of penicillin G/ml for 4 days and then ingested 10(7) CFU of P. aeruginosa per ml of drinking water for 5 days. After GI colonization levels were determined, cyclophosphamide (Cy) was then injected intraperitoneally (i.p.) three times every other day or an antineutrophil monoclonal antibody, RB6-8C5, was injected i.p. once. Dissemination was defined by the presence of P. aeruginosa in spleens of moribund or dead mice. In this mouse model, P. aeruginosa colonizes the GI tract and then disseminates systemically once Cy or RB6-8C5 is administered. The duration and intensity of neutropenia, related to Cy dose, was found to be a means to compare the virulence of different P. aeruginosa strains, as exhibited by comparisons of strains lacking or producing the virulence-enhancing ExoU cytotoxin. The lipopolysaccharide outer core polysaccharide and O side chains were critical in establishing GI colonization, and P. aeruginosa mutants lacking the aroA gene (necessary for synthesizing aromatic amino acids) were able to establish GI colonization but unable to disseminate. Both the colonization and dissemination phases of P. aeruginosa pathogenesis can be studied in this model, which should prove useful for evaluating pathogenesis, therapies, and associated means to control P. aeruginosa nosocomial infections.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Koh AY,Priebe GP,Pier GBdoi
10.1128/IAI.73.4.2262-2272.2005keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
2262-72issue
4eissn
0019-9567issn
1098-5522pii
73/4/2262journal_volume
73pub_type
杂志文章abstract::Stimulated polymorphonuclear leukocytes have been shown by this laboratory to release a reactive-oxygen species (ROS) which is detectable in the supernatant and is capable of oxidizing reduced glutathione and reacting with methionine (Sagone et al., Blood 63:96-104, 1984). This ROS is dependent on H2O2 and heme for it...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.846-849.1984
更新日期:1984-03-01 00:00:00
abstract::Immunization of New Zealand White rabbits with purified visna virus elicited antibody activity demonstrated by passive hemagglutination (PHA), complement fixation (CF), and indirect immunofluorescent tests. The antibody activities of hyperimmune sera and Sephadex G-200 fractions of the sera were studied. It was found ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.6.715-719.1971
更新日期:1971-12-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00718-08
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1055-1060.1992
更新日期:1992-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2290-2299.1998
更新日期:1998-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2624-2627.1989
更新日期:1989-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3097-3105.2004
更新日期:2004-06-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.57.4.1271-1275.1989
更新日期:1989-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.263-267.1977
更新日期:1977-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5329-5336.1998
更新日期:1998-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.3894-3899.2000
更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.4.851-861.1974
更新日期:1974-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.755-763.1982
更新日期:1982-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.7.2861-2867.1997
更新日期:1997-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2962-2965.1990
更新日期:1990-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4963-4967.1999
更新日期:1999-09-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2001-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1209-1214.1988
更新日期:1988-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.589-596.1980
更新日期:1980-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1374-1379.1989
更新日期:1989-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.254-257.1980
更新日期:1980-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2685-2691.2000
更新日期:2000-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00287-10
更新日期:2010-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.8.2301-2305.1989
更新日期:1989-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.1028-1035.1982
更新日期:1982-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.6978-6986.2004
更新日期:2004-12-01 00:00:00