Abstract:
:(DBA/N[female] X CBA/2[male])F1 males have been reported to be deficient in producing antibodies against a number of antigens, including carbohydrates (I. Scher, Adv. Immunol. 35:1-71, 1982). We show that F1 male mice, in contrast to females, made less lipopolysaccharide (LPS)-specific antibodies after immunization with heat-inactivated Pseudomonas aeruginosa and had significantly less naturally occurring LPS-specific antibodies. Furthermore, neutropenic males were 50 to 1,000 times more sensitive to challenge with representative isolates belonging to the seven Fisher immunotypes. Administration to neutropenic F1 males of a human monoclonal antibody specific for the O carbohydrates of P. aeruginosa immunotype 2 LPS or administration of serum from rabbits immunized with heat-inactivated P. aeruginosa immunotype 1 raised the level of resistance to bacterial challenge close to that of females. The results show that the X-linked immunodeficient mouse is an excellent model with which to test the protective efficacy of P. aeruginosa-specific monoclonal antibodies.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Zweerink HJ,Gammon MC,Hutchison CF,Jackson JJ,Pier GB,Puckett JM,Sewell TJ,Sigal NHdoi
10.1128/IAI.56.5.1209-1214.1988subject
Has Abstractpub_date
1988-05-01 00:00:00pages
1209-14issue
5eissn
0019-9567issn
1098-5522journal_volume
56pub_type
杂志文章abstract::Visceral leishmaniasis is a severe and lethal disease caused by the protozoan parasites of the genus Leishmania. In areas where leishmaniasis is endemic, most infected individuals control the infection and remain asymptomatic; chemotherapy of visceral leishmaniasis restores some immunity which protects against relapse...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5559-5566.1999
更新日期:1999-11-01 00:00:00
abstract::Since melanin is considered a virulence factor in Cryptococcus neoformans, its suppression at 37 degrees C has been perplexing. We now show an opposite thermal regulation of superoxide dismutase (SOD), consistent with a compensatory mechanism. Moreover, we demonstrate normal SOD and catalase levels in albino, oxygen-s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4085-4086.1994
更新日期:1994-09-01 00:00:00
abstract::An experimental model of coxsackievirus B3 infection in newborn mice was utilized to examine the protective role of antiviral antibodies and an interferon inducer, polyinosinic acid-polycytidylic acid [poly(I:C)]. Subcutaneous administration to the infected mice of specific antiviral antibodies resulted in significant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.720-727.1982
更新日期:1982-08-01 00:00:00
abstract::A new strategy for rapidly selecting and testing genetic vaccines has been developed, in which a whole genome library is cloned into a bacteriophage lambda ZAP Express vector which contains both prokaryotic (P(lac)) and eukaryotic (P(CMV)) promoters upstream of the insertion site. The phage library is plated on Escher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.167-174.2006
更新日期:2006-01-01 00:00:00
abstract::Toxicity of lipopolysaccharide (LPS) (endotoxin) is, to a large extent, mediated by the activation of monocytes/macrophages and subsequent release of monokines, such as interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha). It is known that LPS binds readily to serum lipoproteins and that LPS-lipoprotein co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.2237-2245.1989
更新日期:1989-07-01 00:00:00
abstract::Female LSH hamsters infected with Treponema pallidum subsp, endemicum before pregnancy or during early pregnancy transmit a form of syphilis to the fetus that is similar to human congenital syphilis. The offspring develops rhinitis, skin rash, failure to thrive, and hepatosplenomegaly. T. pallidum is detectable in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3559-3561.1993
更新日期:1993-08-01 00:00:00
abstract::We have mapped a group of virulence genes of Porphyromonas gingivalis to a single large fragment of the genome. These genes (rgpA, kgp, and hagA) all contain a consensus repeat sequence (HArep). rgpA and kgp encode cysteine proteases with Arg-X and Lys-X specificity, respectively, and hagA encodes a hemagglutinin. Gen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2619-2623.1999
更新日期:1999-05-01 00:00:00
abstract::The role of the spleen in hepatic amoebiasis in hamsters was studied. In hamsters receiving an intrahepatic inoculation of 10(5) trophozoites of axenic Entamoeba histolytica at 7 or 14 days postsplenectomy, the mean weight of metastatic foci increased significantly when compared with sham-splenectomized or intact cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.571-573.1981
更新日期:1981-02-01 00:00:00
abstract::The ability of Toxoplasma gondii tachyzoites to differentiate into latent bradyzoite forms is essential for pathogenesis of clinical disease. We examined the effects of cyclic nucleotides on T. gondii bradyzoite differentiation in vitro. Differentiation of tachyzoites to bradyzoites was measured in an immunofluorescen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.148-153.2001
更新日期:2001-01-01 00:00:00
abstract::Vaccine adjuvants exert critical and unique influences on the quality of immune responses induced during active immunizations. We investigated the mechanisms of action of immunological adjuvants in terms of their requirements for cytokine-mediated pathways for adjuvanticity. Antibody responses potentiated by several a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5329-5336.1998
更新日期:1998-11-01 00:00:00
abstract::We have previously identified two K88ac adhesion receptors (210 and 240 kDa) which are present in membrane preparations from adhesive but not nonadhesive porcine intestinal brush border cells; these adhesin receptors are postulated to be important determinants of the susceptibility of pigs to K88ac+ enterotoxigenic Es...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5404-5410.1994
更新日期:1994-12-01 00:00:00
abstract::Potential vaccine components for protection against atrophic rhinitis in pigs were developed. This was achieved by deletion mutagenesis of the gene encoding the Pasteurella multocida toxin. Four purified toxin derivatives lacking different and widely separated regions in the amino acid sequence were characterized by a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1387-1393.1991
更新日期:1991-04-01 00:00:00
abstract::Cell walls from Streptococcus mutans were prepared by conventional technique and subjected to a series of extraction procedures involving classical protein solvents. The extracted walls contained several non-peptidoglycan amino acids and were also amenable to radiolabeling with [125I]sodium iodide and chloramine T. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.118-126.1980
更新日期:1980-04-01 00:00:00
abstract::Listeria monocytogenes is an intracellular bacterial pathogen which causes bacteremia and has a tropism for the central nervous system and a propensity to cause maternofetal infection. L. monocytogenes has been shown to be an effective prophylactic and a therapeutic vaccine vector for viral and tumor antigens in anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3592-3601.2002
更新日期:2002-07-01 00:00:00
abstract::The American Heart Association supports an association between periodontal diseases and atherosclerosis but not a causal association. This study explores the use of the integrin β6(-/-) mouse model to study the causality. We investigated the ability of a polymicrobial consortium of Porphyromonas gingivalis, Treponema ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01077-15
更新日期:2015-12-01 00:00:00
abstract::Four glycoproteins of Treponema pallidum were identified by intrinsic [14C]glucosamine labeling. Only two glycoproteins were demonstrated in T. phagedenis biotype Reiter with the same technique. Glycoproteins of both treponemes were characterized as antigens and shown to be localized within the outer membranes of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.867-869.1984
更新日期:1984-12-01 00:00:00
abstract::Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli responsible for >80% of all cases. One extreme of UTI is asymptomatic bacteriuria (ABU), which occurs as an asymptomatic carrier state that resembles commensalism. To understand the evolution and molecular me...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02810-14
更新日期:2015-05-01 00:00:00
abstract::A rapid and reproducible method of bacteriocin typing is described based on leakage of ultraviolet light-absorbing material (UVAM), detectable in supernatants of bacteriocin-sensitive cultures, by means of a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard err...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.12-19.1977
更新日期:1977-04-01 00:00:00
abstract::The adipocytokine leptin links nutritional status to immune function. Leptin signaling protects from amebiasis, but the molecular mechanism is not understood. We developed an in vitro model of ameba-host cell interaction to test the hypothesis that leptin prevents ameba-induced apoptosis in host epithelial cells. We d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06140-11
更新日期:2012-05-01 00:00:00
abstract::Rabbit lymphoid cells from spleen, peripheral blood, and peritoneal cavity lacked killer (K)-cell activity against cell lines of rabbit and human origin, including virus-infected human tumor cells. This lack of activity was not affected by antibody concentration, source of antibodies, effector/target cell ratio, or le...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.489-496.1982
更新日期:1982-02-01 00:00:00
abstract::The 23-membered Esx protein family is involved in the host-pathogen interactions of Mycobacterium tuberculosis. These secreted proteins are among the most immunodominant antigens recognized by the human immune system and have thus been used to develop vaccines and immunodiagnostic tests for tuberculosis (TB). Gene pai...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05344-11
更新日期:2011-10-01 00:00:00
abstract::When phagocytosis of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) takes place on the surface of cultured human endothelial cells, the endothelial monolayers are damaged by lysosomal enzymes that are released by the PMN. Because PMN can phagocytose opsonized as well as unopsonized staphylococci on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1455-1460.1987
更新日期:1987-06-01 00:00:00
abstract::Scrub typhus is a potentially lethal infection that is caused by the obligate intracellular bacterium Orientia tsutsugamushi The roles of Toll-like receptor 2 (TLR2) and TLR4 in innate recognition of O. tsutsugamushi have not been elucidated. By overexpression of TLR2 or TLR4 in HEK293 cells, we demonstrated that TLR2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00185-16
更新日期:2016-11-18 00:00:00
abstract::One major outer membrane protein (P1) of Haemophilus influenzae type b (Hib), with an apparent molecular weight of 34,000 (34K) as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), has been shown to be heat modifiable. After heating at 100 degrees C for 5 min in 2% SDS, the P1 protein...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.12.2993-3000.1987
更新日期:1987-12-01 00:00:00
abstract::We report the cloning of the gene encoding the 32-kDa lipoprotein, designated LipL32, the most prominent protein in the leptospiral protein profile. We obtained the N-terminal amino acid sequence of a staphylococcal V8 proteolytic-digest fragment to design an oligonucleotide probe. A Lambda-Zap II library containing E...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2276-2285.2000
更新日期:2000-04-01 00:00:00
abstract::A GroEL-like protein from Bordetella pertussis was purified. This protein was found to have the tetradecameric subunit structure typical of the GroEL family of proteins and to contain epitopes similar to those of other members of this family, including a human GroEL-like protein. Active immunization of neonatal mice w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1417-1422.1991
更新日期:1991-04-01 00:00:00
abstract::Cryptosporidial infections in severe combined immune deficient (SCID) mice produce a chronic disease state which in the later stages leads to extraintestinal involvement and hepatic dysfunction. To further characterize the infection dynamics in this model and monitor the changes in the hepatic system, a dose titration...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1691-1695.1994
更新日期:1994-05-01 00:00:00
abstract::We studied seven strains of group G streptococci isolated from clinically severe bacteremic infections in six intravenous drug abusers. These group G strains multiplied luxuriantly in fresh human blood. On electron microscopy, they exhibited surface fibrillae similar to those observed in M-protein-rich group A strepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.753-757.1987
更新日期:1987-03-01 00:00:00
abstract::A2G mice are genetically resistant to lethal infection with neurotropic and pneumotropic influenza viruses. A possible immunological explanation for this resistance was sought by assessing the effect of cyclophosphamide and X irradiation immunosuppression on the infection of A2G mice with lethal doses of neurovirulent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.576-587.1975
更新日期:1975-03-01 00:00:00
abstract::Variable subregions within the variant surface glycoprotein (VSG) coat displayed by African trypanosomes are predicted sites for T- and B-cell recognition. Hypervariable subregion 1 (HV-1) is localized to an internal amphipathic alpha helix in VSG monomers and may have evolved due to selective pressure by host T-cell ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00729-08
更新日期:2009-01-01 00:00:00