Purification and immunological characterization of a GroEL-like protein from Bordetella pertussis.

Abstract:

:A GroEL-like protein from Bordetella pertussis was purified. This protein was found to have the tetradecameric subunit structure typical of the GroEL family of proteins and to contain epitopes similar to those of other members of this family, including a human GroEL-like protein. Active immunization of neonatal mice with the B. pertussis GroEL-like protein provided little protection against an aerosol challenge with B. pertussis. Antibodies to this protein were elicited in mice by a standard diphtheria-tetanus-pertussis (DTP) vaccine but not by an experimental acellular pertussis vaccine. Since the Bordetella GroEL-like protein was found to contain epitopes similar to those on the mammalian analog, the potential exists that vaccination with standard DTP vaccines may induce antibodies which react with the mammalian GroEL analog.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Burns DL,Gould-Kostka JL,Kessel M,Arciniega JL

doi

10.1128/IAI.59.4.1417-1422.1991

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

1417-22

issue

4

eissn

0019-9567

issn

1098-5522

journal_volume

59

pub_type

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