Abstract:
:Expression of the Vwa+ phenotype of Yersinia pestis in vitro is known to reflect maximum induction of virulence (or V and W antigens) at 37 degrees C with concomitant restriction of cell division. Both phenomena are potentiated by 20 mM Mg2+ and prevented by cultivation at 26 or 37 degrees C with 2.5 mM Ca2+. We have now compared this classic plasmid-mediated phenotype with those of Vwa+ Yersinia pseudotuberculosis and Yersinia enterocolitica which, unlike Y. pestis, produce ancillary outer membrane peptides unrelated to the V and W antigens. All of 10 wild-type strains of Y. enterocolitica (serotypes O:3, O:4,32, O:8, O:9, O:15, and O:21) exhibited a nutritional requirement for Ca2+ at 37 degrees C and produced significant V antigen. Like Y. pseudotuberculosis, autoagglutination of Vwa+ Y. enterocolitica was dependent upon prior growth at 37 degrees C but was not influenced by Ca2+. Autoagglutination of Y. pestis was never observed. Resistance of Y. enterocolitica to 10% human serum was typically dependent upon prior growth at 37 degrees C, either with or without added Ca2+, and carriage of a Vwa plasmid. In contrast, serum resistance of Y. pseudotuberculosis was temperature but not plasmid dependent and that of Y. pestis was constitutive.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Perry RD,Brubaker RRdoi
10.1128/IAI.40.1.166-171.1983subject
Has Abstractpub_date
1983-04-01 00:00:00pages
166-71issue
1eissn
0019-9567issn
1098-5522journal_volume
40pub_type
杂志文章abstract::Virulent Streptococcus suis serotype 2 strains are invasive extracellular bacteria causing septicemia and meningitis in piglets and humans. One objective of this study was to elucidate the function of complement in innate immune defense against S. suis. Experimental infection of wild-type (WT) and C3(-/-) mice demonst...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-14
更新日期:2014-06-01 00:00:00
abstract::Carbon storage regulator A (CsrA) is an RNA binding protein that has been characterized in many bacterial species to play a central regulatory role by modulating several metabolic processes. We recently showed that a homolog of CsrA in Borrelia burgdorferi (CsrA(Bb), BB0184) was upregulated in response to propagation ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00882-10
更新日期:2011-02-01 00:00:00
abstract::Cells of the laboratory strain 3153A of Candida albicans can be stimulated to undergo high-frequency phenotypic switching by a low dose of UV. We have compared the adhesive properties of cells exhibiting the basic original smooth (o-smooth) phenotype and three switch phenotypes (star, irregular wrinkle, and revertant ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1328-1335.1994
更新日期:1994-04-01 00:00:00
abstract::The bacterium Neisseria meningitidis is the causative agent of meningitis and sepsis. A generally effective vaccine against N. meningitidis serogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion of N. meningitidis wild-ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00846-07
更新日期:2007-12-01 00:00:00
abstract::Ehrlichia chaffeensis is an obligately intracellular gram-negative bacterium and is the etiologic agent of human monocytic ehrlichiosis (HME). Although E. chaffeensis induces the generation of several cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglycan. Bioinfomatic analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00056-08
更新日期:2008-08-01 00:00:00
abstract::Expression of ace (adhesin to collagen of Enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions, such as in the presence of serum, bile salts, urine, and collagen and at 46 °C. However, the mechanism of ace/Ace regula...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02587-14
更新日期:2015-01-01 00:00:00
abstract::Pneumolysin, the cholesterol-dependent cytolysin of Streptococcus pneumoniae, induces inflammatory and apoptotic events in mammalian cells. Toll-like receptor 4 (TLR4) confers resistance to pneumococcal infection via its interaction with pneumolysin, but the underlying mechanisms remain to be identified. In the presen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6479-6487.2005
更新日期:2005-10-01 00:00:00
abstract::CD8 T cells are effective mediators of specific immunity to infection by Listeria monocytogenes, a bacterial pathogen that initially infects macrophages in the spleen and liver and subsequently spreads to hepatocytes and unidentified parenchymal cells in the spleen. To identify the in vivo target cells of L. monocytog...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3632-3640.1996
更新日期:1996-09-01 00:00:00
abstract::Active immunization with Porphyromonas gingivalis whole-cell preparations has been shown to prevent P. gingivalis infection and oral bone loss. Employing passive antibody transfer and opsonization, we demonstrate with this study that immunization-elicited P. gingivalis-specific immunoglobulin G facilitates clearance o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2408-2411.2004
更新日期:2004-04-01 00:00:00
abstract::Although there appears to be little if any role for specific antibodies in protection against intracellular bacteria, such as the model pathogen F. tularensis live vaccine strain (LVS), the role of B cells themselves in primary and secondary infection with such bacteria has not been examined directly. We show here tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6002-6007.1999
更新日期:1999-11-01 00:00:00
abstract::Classical and El Tor strains of Vibrio cholerae were examined for production of bacteriophage or bacteriophage-related material by electron microscopy of mitomycin C-induced cultures. The strains were also tested for pathogenicity by using the ligated ileal loops of adult rabbits. Of the 27 strains tested, 25 showed a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.1.4.417-420.1970
更新日期:1970-04-01 00:00:00
abstract::Naturally occurring antibodies against [Gal alpha-1,3-Gal] structures (anti-Gal antibodies) are the primary effectors of human hyperacute rejection (HAR) of nonhuman tissue. Unlike most mammals, humans lack a functional alpha-1,3-galactosyltransferase (GalT) gene and produce abundant anti-Gal antibodies, putatively in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6215-6222.2002
更新日期:2002-11-01 00:00:00
abstract::The growth of Eimeria vermiformis within cultured murine fibroblastlike (L-929) or rat epithelial-like (RATEC) cells was inhibited by treatment of the cells with the appropriate recombinant gamma interferon. The effect was apparent as a reduction in both the initial numbers of intracellular sporozoites and, to a much ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.2.580-586.1991
更新日期:1991-02-01 00:00:00
abstract::The type III secretion system (T3SS) plays a crucial role in the pathogenesis of many Gram-negative bacteria, including Edwardsiella tarda, an important fish pathogen. Within the E. tarda T3SS, there are three proteins (EsaB/EsaL/EsaM) that are homologous to proteins present in many other bacteria, including SpiC/SsaL...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00322-17
更新日期:2017-08-18 00:00:00
abstract::Bacterial infection with group B Streptococcus (GBS) represents a prominent threat to neonates and fetuses in the Western world, causing severe organ damage and even death. To improve current therapeutic strategies and to investigate new approaches, an appropriate in vivo model to study the immune response of a human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01235-12
更新日期:2013-05-01 00:00:00
abstract::In the progression of the life cycle of Plasmodium falciparum, a small proportion of asexual parasites differentiate into male or female sexual forms called gametocytes. Just like their asexual counterparts, gametocytes are contained within the infected host's erythrocytes (RBCs). However, unlike their asexual partner...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00775-18
更新日期:2019-05-21 00:00:00
abstract::Several previous findings have suggested that the cationic nature of lysozyme is a major factor in its bactericidal activity. Since a number of cationic proteins or peptides have been reported to cause membrane damage in bacteria, we investigated the effect of lysozyme on glucose fermentation and intracellular pH and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.2.638-644.1991
更新日期:1991-02-01 00:00:00
abstract::Synthetic lipid A analogs which have two amide-bound and two ester-bound (R)-3-hydroxytetradecanoyl groups at the C-2 and -2' and C-3 and -3' positions of beta(1-6)glucosamine disaccharide mono- or diphosphates showed high activities in most in vitro assays, and the lethality of a diphosphate derivative to galactosami...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.293-296.1984
更新日期:1984-07-01 00:00:00
abstract::Proteins were extracted at pH 2.5 from carefully washed brain homogenates of patients with subacute sclerosing panencephalitis. Immunoglobulin G was isolated from the extracts by gel filtration and immunoadsorbent chromatography and was 125I radioiodinated. The labeled immunoglobulin G was reacted with purified measle...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.202-210.1979
更新日期:1979-04-01 00:00:00
abstract::Purified and concentrated Venezuelan equine encephalitis (VEE) virus derived from tissue cultures, rendered noninfectious by ionizing radiation with retention of in vitro serological activity, also retained a high level of immunogenicity. In mice, fluid vaccines afforded excellent protection against lethal challenge w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.4.574-579.1971
更新日期:1971-04-01 00:00:00
abstract::Proteins from human polymorphonuclear leukocyte granules were extracted with 0.2 M acetate, pH 4.0, and fractionated by Sephadex G-100 column chromatography. The fractions demonstrated selective bactericidal action against a deep rough cell wall mutant of Escherichia coli O111:B4 with rough lipopolysacharide and cell ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.3.587-591.1979
更新日期:1979-03-01 00:00:00
abstract::No effective therapy exists for Cryptosporidium parvum, a coccidial protozoan parasite that causes severe diarrhea in patients with AIDS. The role of microtubules in parasite invasion of host cells was investigated by incubating 10(7) oocysts with a HT 29.74 cell line for 24 h in the presence of microtubule-disrupting...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4888-4890.1993
更新日期:1993-11-01 00:00:00
abstract::Specific proteolytic destruction of the human chemotaxin, C5a, is a property of group A and B streptococcal pathogens. Here we show that virulent group G streptococci from human sources also express C5a peptidase activity. The enzyme responsible for this activity is approximately the same size as and is antigenically ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2305-2310.1991
更新日期:1991-07-01 00:00:00
abstract::Toxin preparations were obtained by growing Clostridium difficile VPI strain 10463 in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days. The initial step of the purification scheme involved ultrafiltration through an XM-100 membrane filter. Two toxic activities, designated toxins A and B, were se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1032-1040.1982
更新日期:1982-03-01 00:00:00
abstract::Previous studies have shown that tumor necrosis factor alpha (TNF-alpha) plays a pathophysiologic role in sepsis induced in rat pups by group B streptococci (GBS). In this model, TNF-alpha is also partially responsible for the induction of interleukin-6 (IL-6). The present study was undertaken to investigate the role ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4997-5002.1994
更新日期:1994-11-01 00:00:00
abstract::Alveolar bone destruction is a characteristic feature of periodontitis. Treponema denticola is known to be involved in periodontitis. To elucidate the role of T. denticola in alveolar bone destruction in periodontitis, the effects of lipooligosaccharide (LOS) from T. denticola on osteoclast formation and on expression...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.226-233.2003
更新日期:2003-01-01 00:00:00
abstract::Escherichia coli is the most common cause of urinary tract infection. Elevated blood and urine interleukin-6 (IL-6) levels have been shown in inflammatory urinary tract diseases. The role of IL-6 in mediating the urodynamic dysfunction in response to E. coli-induced urinary tract infection has not yet been fully eluci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00013-09
更新日期:2009-08-01 00:00:00
abstract::The effects of 1,25-dihydroxy vitamin D3 and gamma interferon on the phenotypic changes associated with monocyte maturation in vitro were investigated. Human monocytes separated from peripheral blood mononuclear cell populations by adherence to plastic were cultured for 7 days on glass. Immunocytological analysis was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2017-2020.1987
更新日期:1987-09-01 00:00:00
abstract::We previously reported that infection by Fusobacterium nucleatum strongly induced the expression of both human beta-defensin 2 (HBD-2) and HBD-3 by gingival epithelial cells. The aim of this study was to characterize the pattern recognition receptors (PRRs) and regulatory mechanisms involved in the induction of HBD-2 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00449-08
更新日期:2009-03-01 00:00:00
abstract::An antigen recognized by monoclonal antibody specific for Bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. The purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.366-371.1986
更新日期:1986-08-01 00:00:00