Abstract:
:Virulent Streptococcus suis serotype 2 strains are invasive extracellular bacteria causing septicemia and meningitis in piglets and humans. One objective of this study was to elucidate the function of complement in innate immune defense against S. suis. Experimental infection of wild-type (WT) and C3(-/-) mice demonstrated for the first time that the complement system protects naive mice against invasive mucosal S. suis infection. S. suis WT but not an unencapsulated mutant caused mortality associated with meningitis and other pathologies in C3(-/-) mice. The capsule contributed also substantially to colonization of the upper respiratory tract. Experimental infection of C3(-/-) mice with a suilysin mutant indicated that suilysin expression facilitated an early disease onset and the pathogenesis of meningitis. Flow cytometric analysis revealed C3 antigen deposition on the surface of ca. 40% of S. suis WT bacteria after opsonization with naive WT mouse serum, although to a significantly lower intensity than on the unencapsulated mutant. Ex vivo multiplication in murine WT and C3(-/-) blood depended on capsule but not suilysin expression. Interestingly, S. suis invasion of inner organs was also detectable in C5aR(-/-) mice, suggesting that chemotaxis and activation of immune cells via the anaphylatoxin receptor C5aR is, in addition to opsonization, a further important function of the complement system in defense against mucosal S. suis infection. In conclusion, we unequivocally demonstrate here the importance of complement against mucosal S. suis serotype 2 infection and that the capsule of this pathogen is also involved in escape from complement-independent immunity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Seitz M,Beineke A,Singpiel A,Willenborg J,Dutow P,Goethe R,Valentin-Weigand P,Klos A,Baums CGdoi
10.1128/IAI.00080-14subject
Has Abstractpub_date
2014-06-01 00:00:00pages
2460-71issue
6eissn
0019-9567issn
1098-5522pii
IAI.00080-14journal_volume
82pub_type
杂志文章abstract::Cholera enterotoxin and the related heat-labile enterotoxins of Escherichia coli enter their target cells through noncoated vesicles, but how the toxins are processed intracellularly and how they get to their targeted enzyme, adenylate cyclase, remain to be defined. Brefeldin A, an inhibitor of the trans-Golgi network...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3282-3286.1993
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abstract::Internalization of Staphylococcus aureus in bovine endothelial cells (BEC) is increased by tumor necrosis factor alpha stimulation and NF-κB activation. Because the phosphoinositide-3-kinase (PI3K)-Akt signaling pathway also modulates NF-κB activity, we considered whether the internalization of S. aureus by BEC is ass...
journal_title:Infection and immunity
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doi:10.1128/IAI.05303-11
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1110-1113.1984
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abstract::Malaria toxin causes hypoglycemia and induction of tumor necrosis factor. Extracts of parasitized erythrocytes which were coeluted and copurified with one of the two subtypes of mammalian insulin-mimetic inositolphosphoglycans similarly induced fibroblast proliferation in the absence of serum. In addition, induction o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3438-3441.1996
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1360-1368.1990
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5231-5242.1999
更新日期:1999-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02621-14
更新日期:2015-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4868-4873.2004
更新日期:2004-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.363-365.1995
更新日期:1995-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3330-3335.2002
更新日期:2002-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5399-5405.1998
更新日期:1998-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.501-506.1984
更新日期:1984-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00386-05
更新日期:2006-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1520-1527.1994
更新日期:1994-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6839-6845.2002
更新日期:2002-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00022-06
更新日期:2006-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1092-1101.1983
更新日期:1983-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.887-894.1982
更新日期:1982-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3343-3345.1991
更新日期:1991-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4626-4631.1994
更新日期:1994-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00646-18
更新日期:2019-03-25 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.649-654.1981
更新日期:1981-05-01 00:00:00
abstract::Development of hemolytic uremic syndrome (HUS) after infection by Shigella dysenteriae 1 or enterohemorrhagic Escherichia coli has been associated with the production of Shiga toxins (verotoxins). The putative target of Shiga toxins in HUS is the renal microvascular endothelium. This report shows that preincubation of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3886-3891.1993
更新日期:1993-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.873-879.1979
更新日期:1979-09-01 00:00:00
abstract::Macrophage recognition of Salmonella enterica serovar Typhimurium leads to a cascade of signaling events, including the activation of Src family and Syk kinases and the production of reactive oxygen species (ROS), which are critical for host innate defense during early stages of bacterial infection. ROS production dep...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00758-17
更新日期:2018-03-22 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01388-08
更新日期:2009-05-01 00:00:00
abstract::The vast majority of cases of gram-negative meningitis in neonates are caused by K1-encapsulated Escherichia coli. The role of the K1 capsule in the pathogenesis of E. coli meningitis was examined with an in vivo model of experimental hematogenous E. coli K1 meningitis and an in vitro model of the blood-brain barrier....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3566-3570.1999
更新日期:1999-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.333-336.1978
更新日期:1978-07-01 00:00:00