Abstract:
:We demonstrate that a mucoid, alginate-producing strain of Pseudomonas aeruginosa isolated from the lungs of a cystic fibrosis (CF) patient secretes multiple enzymes with nucleoside diphosphate kinase (Ndk), ATPase, adenylate kinase, 5'-nucleotidase, and ATP-modifying enzymatic activities. The secretion is triggered at high cell density and in complex media but is greatly reduced when the mucoid cells are grown in mineral salts media or in presence of 5.0 mM Ca2+ or Mg2+. Interestingly, the secretion is triggered primarily in the mucoid CF isolate of strain 8821M (or in strain FRD1) but not in a nonmucoid laboratory strain, PAO1. The purified secreted Ndk shows 100% match in its N-terminal amino acid sequence with that of purified intracellular Ndk and demonstrates similar enzymatic properties. The N-terminal sequence of the purified ATPase isolated from an ndk knockout mutant shows its identity with that of the heat shock chaperonin Hsp60. During fractionation, the flowthrough fraction from a Mono Q column demonstrates the presence of 5'-nucleotidase, adenylate kinase, and a putative ATP reductase activity. These fractions demonstrate high cytotoxic activities for murine peritoneal primary macrophages which can be further stimulated in the presence of ATP or inhibited by pretreatment of macrophages with oxidized ATP (oATP). The cytotoxicity associated with ATP-induced stimulation is believed to be due to activation of macrophage surface-associated P2Z (P2X7) receptors, which are one of the purinergic receptors responsible for pore formation on macrophage membrane. Blocking of these receptors by pretreatment with oATP blocks ATP-induced macrophage cell death. Thus mucoid P. aeruginosa cells elaborate enzymes that modulate the external ATP levels of macrophages, thereby modulating macrophage cell death through P2Z receptor activation. Evidence for the presence of secreted cytotoxic agents that act independently of P2Z receptor activation is also presented.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Zaborina O,Misra N,Kostal J,Kamath S,Kapatral V,El-Idrissi ME,Prabhakar BS,Chakrabarty AMdoi
10.1128/IAI.67.10.5231-5242.1999keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
5231-42issue
10eissn
0019-9567issn
1098-5522journal_volume
67pub_type
杂志文章abstract::Ultrastructural immunoperoxidase studies were done in spinal cords of mice infected with wild type vesicular stomatitis virus or its temperature-sensitive (ts) mutant G31. Infected neurons showed subplasmalemmal staining of viral antigen and staining of viral particles budding from the neuronal membrane in wild-type v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.276-281.1979
更新日期:1979-04-01 00:00:00
abstract::Serotypes A and B of the relapsing fever spirochete Borrelia turicatae produce different disease manifestations in infected mice. Whereas serotype B causes more severe arthritis and reaches higher densities in the blood of mice than serotype A, serotype A invades the central nervous system earlier than serotype B duri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4637-4645.1999
更新日期:1999-09-01 00:00:00
abstract::Listeria monocytogenes, isolated from outbreaks in either human or nonhuman primate populations, was administered orally at doses ranging from 10(6) to 10(10) CFU. Four of 10 treated animals delivered stillborn infants. L. monocytogenes was isolated from fetal tissue, and the pathology was consistent with L. monocytog...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1574-1579.2003
更新日期:2003-03-01 00:00:00
abstract::Strain superinfection occurs when a second pathogen strain infects a host already carrying a primary strain. Anaplasma marginale superinfection occurs when the second strain carries a variant repertoire different from that of the primary strain, and the epidemiologic consequences depend on the relative efficiencies of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01518-08
更新日期:2009-04-01 00:00:00
abstract::Henle 407 and HeLa cells were compared as hosts for Shigella dysenteriae at a low multiplicity of infection. Efficiency of attachment and invasion without centrifugation, as well as selectivity for pathogenic over nonpathogenic S. dysenteriae without Congo red, were much greater for Henle 407 cells than for HeLa cells...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2401-2403.1990
更新日期:1990-07-01 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for large numbers of postsurgical nosocomial infections across the United States and worldwide. Propofol anesthesia is widely used in surgery and in intensive care units, and recent evidence indicates that even brief exposure to propofol can substantial...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00097-17
更新日期:2017-06-20 00:00:00
abstract::Bordetella pertussis was grown in iron (Fe)-free defined medium to limit the growth of the organism. Doubling times of the Fe-starved organism increased by approximately 1 h, and a 40% reduction in the final extent of growth in Fe-depleted medium was observed. Under these conditions, a hydroxamate siderophore named bo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.117-123.1992
更新日期:1992-01-01 00:00:00
abstract::Chronic helminth infections are known to be associated with the modulation of antigen-specific T-cell responses. Strongyloides stercoralis infection is characterized by the downmodulation of antigen-specific Th1 and Th17 responses and the upregulation of Th2 and Th9 responses. Immune homeostasis is partially maintaine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00469-19
更新日期:2019-11-18 00:00:00
abstract::Cells of Yersinia pestis strain EV76 are known to cease growth after a shift from 26 to 37 degrees C in neutral Ca2+-deficient medium; this effect is potentiated by Mg2+. With 2.5 mM Mg2+ and no added Ca2+, restriction was relaxed at elevated pH at which maximum cell yields occurred in the presence of 20 mM exogenous ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.953-959.1982
更新日期:1982-12-01 00:00:00
abstract::Coxiella burnetii, the agent of Q fever, enters human monocytes through alpha(v)beta(3) integrin and survives inside host cells. In addition, C. burnetii stimulates the synthesis of inflammatory cytokines including tumor necrosis factor (TNF) by monocytes. We studied the role of the interaction of C. burnetii with THP...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5673-5678.2000
更新日期:2000-10-01 00:00:00
abstract::Vibrio cholerae accessory colonization factor genes (acfA, B, C, and D) are required for efficient intestinal colonization. Expression of acf genes is under the control of a regulatory cascade that also directs the synthesis of cholera toxin and proteins involved in the biogenesis of the toxin-coregulated pilus. The g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3289-3298.1994
更新日期:1994-08-01 00:00:00
abstract::Salivary gland lysates of the sand fly Lutzomyia longipalpis markedly enhance the course of infection with Leishmania major in mice. Here we examine various parameters of this phenomenon. The exacerbative effect of L. longipalpis salivary gland lysates occurred in five different mouse strains; however, the character o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1592-1598.1991
更新日期:1991-05-01 00:00:00
abstract::We previously showed that cytotoxic necrotizing factor 1 (CNF1) contributes to Escherichia coli K1 invasion of human brain microvascular endothelial cells (HBMEC) and interacts with the receptor on the surface of HBMEC. CNF1 is the cytoplasmic protein, and it remains incompletely understood how CNF1 is secreted across...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00674-09
更新日期:2010-02-01 00:00:00
abstract::The presence of choriogonadotropin- and alpha-subunit-like materials in two species of bacteria identified as Staphylococcus simulans and Streptococcus faecalis have been demonstrated by the indirect fluorescein-labeled and the indirect peroxidase-labeled immunocytochemical techniques, utilizing antiserum for human ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.487-494.1981
更新日期:1981-01-01 00:00:00
abstract::Group A streptococcal cell walls inhibited phagocytosis of polystyrene latex particles by rat peritoneal macrophages in vitro. This inhibition was not accompanied by a measurable loss of cell viability. Group D streptococcal cell walls were relatively ineffective in altering phagocytosis. The effectiveness of group A ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.258-261.1978
更新日期:1978-04-01 00:00:00
abstract::The pneumocandins are potent antifungal agents of the echinocandin class which are under development for use as broad-spectrum antimycotic therapy. One important consideration for any new therapeutic class for treating serious fungal infections is the potential for drug resistance development. In this study we have is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3244-3251.1996
更新日期:1996-08-01 00:00:00
abstract::Analyses of chemical composition in whole cells of Rickettsia tsutsugamushi were performed and compared with those of the other rickettsiae and gram-negative bacteria. The results indicated that R. tsutsugamushi does not contain detectable amounts of 3-deoxy-D-mannooctulosonic acid, heptose, muramic acid, or glucosami...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2290-2292.1987
更新日期:1987-09-01 00:00:00
abstract::The type-specific antigens of group B type Ic (old designation type Ii) streptococci were extracted, purified, and characterized by serological and chemical methods. The Ia antigen, shared by types Ia and Ic, is a polysaccharide composed of 69% galactose and 25% glucosamine (i.e., 31% N-acetyl-glucosamine). However, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.596-604.1971
更新日期:1971-11-01 00:00:00
abstract::Bacteria exhibit extensive genetic heterogeneity within species. In many cases, these differences account for virulence properties unique to specific strains. Several such loci have been discovered in the genome of the type b serotype of Haemophilus influenzae, a human pathogen able to cause meningitis, pneumonia, and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1098-1108.2003
更新日期:2003-03-01 00:00:00
abstract::Pertussis toxin is secreted from Bordetella pertussis with the assistance of the Ptl transport system, a member of the type IV family of macromolecular transporters. The S1 subunit and the B oligomer combine to form the holotoxin prior to export from the bacterial cell, although the site of assembly is not known. To b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1193-1201.2002
更新日期:2002-03-01 00:00:00
abstract::Staphylococcus aureus Newman with an insertion mutation in clfB, the gene encoding clumping factor B, only marginally decreased infection rate (P>0.05) in rats with experimental endocarditis. In contrast, clfB complementation on a multicopy plasmid significantly increased infectivity (P<0.05) over the deleted mutants....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5443-5446.2000
更新日期:2000-09-01 00:00:00
abstract::The P2 porin protein is the most abundant protein in the outer membrane of Haemophilus influenzae type b (Hib). Biochemical and immunochemical techniques were used to characterize the P2 proteins from a number of different Hib strains. P2 proteins from Hib outer membrane vesicles were resolved by sodium dodecyl sulfat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3270-3275.1989
更新日期:1989-11-01 00:00:00
abstract::Mycoplasma gallisepticum is an avian respiratory and reproductive tract pathogen that has a significant economic impact on the poultry industry worldwide. Although membrane proteins of Mycoplasma spp. are thought to play crucial roles in host interactions, very few have had their biochemical function defined. In this ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-19
更新日期:2019-08-21 00:00:00
abstract::We evaluated sexual recombination in the apicomplexan parasite Theileria parva using genome-wide marker analysis of haploid sporozoite populations obtained from infected Rhipicephalus appendiculatus ticks. Analysis of 231 parasite clones derived by in vitro infection of bovine lymphocytes revealed 48 distinct combinat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00472-06
更新日期:2006-10-01 00:00:00
abstract::A rapid and reproducible method of bacteriocin typing is described based on leakage of ultraviolet light-absorbing material (UVAM), detectable in supernatants of bacteriocin-sensitive cultures, by means of a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard err...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.12-19.1977
更新日期:1977-04-01 00:00:00
abstract::The expression of the Shigella flexneri chromosomal aerobactin genes during growth of the bacterium within tissue culture cells was assayed. During intracellular growth, aerobactin promoter activity was repressed relative to the level observed in bacteria grown extracellularly, even when the bacteria had been starved ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.818-821.1997
更新日期:1997-02-01 00:00:00
abstract::The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli. CpxR, the cytoplasmic response regulator, was shown previously to be involved in repression of the expression of the lspB-lspA2 operon (M. Labandeira-Re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00678-10
更新日期:2010-11-01 00:00:00
abstract::Attachment of Fusobacterium nucleatum to various oral surfaces is mediated by several adhesins anchored on its outer surface. Monoclonal antibodies (MAbs) were prepared and used to identify the putative galactose-binding adhesin of F. nucleatum PK1594. Four unique MAbs, 8G7, 26B9, 28G11, and 29D4, were isolated on the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.5231-5237.1997
更新日期:1997-12-01 00:00:00
abstract::Escherichia coli strain RDEC-1 causes a diarrheagenic infection in rabbits with AF/R1 fimbriae, which have been identified as an important colonization factor in RDEC-1 adherence leading to disease. The AF/R1-mediated RDEC-1 adherence model has been used as a model systems for E. coli diarrheal diseases. In this study...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.640-649.2001
更新日期:2001-02-01 00:00:00
abstract::The she gene of Shigella flexneri 2a, which also harbors the internal enterotoxin genes set1A and set1B (F. R. Noriega, GenBank accession no. U35656, 1995) encodes a homolog of the virulence-related immunoglobulin A (IgA) protease-like family of secreted proteins, Tsh, EspC, SepA, and Hap, from an avian pathogenic Esc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.11.4606-4614.1997
更新日期:1997-11-01 00:00:00